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  1. Article ; Online: Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureus

    Vishu Chaudhary / Priya Katyal / Harsh Panwar / Anil Kumar Puniya / Anuj Kumar Poonia

    Environment Conservation Journal, Vol 23, Iss 1&

    2022  Volume 2

    Abstract: In the present study, Monascus red biopigment produced by solid-state fermentation was evaluated for its anti-microbial and antioxidative potential. The antibacterial activity through Scanning Electron Microscopy against Bacillus cereus, Escherichia coli, ...

    Abstract In the present study, Monascus red biopigment produced by solid-state fermentation was evaluated for its anti-microbial and antioxidative potential. The antibacterial activity through Scanning Electron Microscopy against Bacillus cereus, Escherichia coli, and Staphylococcus aureus was found to show morphological damage in some cells, as evidenced by the outflow of cell contents, deep craters, burst cells, and cell death at concentration of 200 µg/ml of biopigment. Moreover, antibacterial activity through agar well diffusion method against Bacillus cereus, Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa was in range of 2-6 mm by varying concentration of biopigment from 1 to 20 mg/ml.Next, the antifungal activity of the extracted biopigment was in the range of 2-9 mm for Aspergillus flavus, Fusarium oxysporum and Alternaria alternata. In addition, antioxidant efficacy of red biopigment through DPPH, ABTS and FRAP assay results was found to show 59.69 %, 91.1 %, and 15.22 % free radical scavenging activity. The results of this study revealed that red biopigment has potential to modulate the antimicrobial and antioxidative activity.
    Keywords Antimicrobial ; Antioxidative ; Food colorants ; Monascus purpureus ; Red Biopigment ; Environmental sciences ; GE1-350
    Subject code 571
    Language English
    Publishing date 2022-02-01T00:00:00Z
    Publisher Action for Sustainable Efficacious Development and Awareness
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Effect of β-glucan fortification on physico-chemical, rheological, textural, colour and organoleptic characteristics of low fat dahi

    Bhaskar, Dadi / Sunil Kumar Khatkar / Rekha Chawla / Harsh Panwar / Swati Kapoor

    Journal of food science and technology. 2017 Aug., v. 54, no. 9

    2017  

    Abstract: Fortified low fat functional dahi prepared by supplementing skimmed buffalo milk with β-glucan (0.25, 0.50, 0.75 and 1.0%) was assessed for physico-chemical, rheological, textural, colour and sensorial characteristics. Total solids in dahi increased ... ...

    Abstract Fortified low fat functional dahi prepared by supplementing skimmed buffalo milk with β-glucan (0.25, 0.50, 0.75 and 1.0%) was assessed for physico-chemical, rheological, textural, colour and sensorial characteristics. Total solids in dahi increased significantly with the increase in β-glucan concentration, however, values of fat, protein and ash varied non-significantly upon β-glucan addition in dahi. β-glucan at 0.5% solids levels, produced low fat dahi with superior quality, less whey separation and good textural properties than the samples containing other levels. Syneresis and viscosity was positively affected with the addition of β-glucan till 0.5% level and higher concentration caused destabilization of the product. Fortified dahi showed greater firmness and consistent than control dahi sample. The addition of 0.5% level of β-glucan also imparted significantly better instrumental color values and sensory scores with attractive or natural dahi color when compared to control dahi samples and other dahi samples prepared with different levels of β-glucan.
    Keywords beta-glucans ; buffalo milk ; color ; firmness ; texture ; total solids ; viscosity ; whey
    Language English
    Dates of publication 2017-08
    Size p. 2684-2693.
    Publishing place Springer India
    Document type Article
    ZDB-ID 242498-8
    ISSN 0975-8402 ; 0022-1155
    ISSN (online) 0975-8402
    ISSN 0022-1155
    DOI 10.1007/s13197-017-2705-6
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2636: Show issues

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  3. Article: Assessment of the antibiofilm and antiquorum sensing activities of Eucalyptus globulus essential oil and its main component 1,8-cineole against methicillin-resistant Staphylococcus aureus strains

    Merghni, Abderrahmen / Emira Noumi / Harsh Panwar / Maha Mastouri / Mejdi Snoussi / Neyla Dridi / Ons Hadded / Ozgur Ceylan

    Microbial pathogenesis. 2018 May, v. 118

    2018  

    Abstract: Antibacterial resistance is an increasingly serious threat to global public health. The search for new anti-infection agents from natural resources, with different mode of actions and competitive effects became a necessity. In this study, twenty height ... ...

    Abstract Antibacterial resistance is an increasingly serious threat to global public health. The search for new anti-infection agents from natural resources, with different mode of actions and competitive effects became a necessity. In this study, twenty height methicillin-resistant Staphylococcus aureus (MRSA) strains were investigated for their biofilm formation ability. Subsequently, the antibiofilm effects of Eucalyptus globulus essential oil and its main component 1,8-cineole, against MRSA, as well as their antiquorum sensing potential, were evaluated. Our results displayed the potent efficacy of both E. globulus essential oil and 1,8-cineole against the development of biofilms formed by the methicillin-resistant strains. Additionally, E. globulus essential oil showed more potent of anti-QS activity, even at a low concentration, when compared to 1,8-cineole. All these property of tested agents may pave the way to prevent the development of biofilm formation by MRSA and subsequently the spreading of nosocomial infection.
    Keywords antibiotic resistance ; biofilm ; cineole ; cross infection ; essential oils ; Eucalyptus globulus ; mechanism of action ; methicillin ; methicillin-resistant Staphylococcus aureus ; natural resources ; public health
    Language English
    Dates of publication 2018-05
    Size p. 74-80.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 632772-2
    ISSN 1096-1208 ; 0882-4010
    ISSN (online) 1096-1208
    ISSN 0882-4010
    DOI 10.1016/j.micpath.2018.03.006
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2136: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article: Inhibitory effects of lactobacilli of goat's milk origin against growth and biofilm formation by pathogens: an in vitro study

    Singh, Niharika / Anil Kumar Puniya / Brij Pal Singh / Chetan Sharma / Gunjan Goel / Harsh Panwar / Namita Rokana / Rohini Devidas Gulhane / Sampan Attri

    Food bioscience. 2018 Apr., v. 22

    2018  

    Abstract: This study investigated the effects of lactic acid bacteria (LAB) of goat's milk origin on growth and biofilm formation of two pathogenic strains viz. Pseudomonas aeruginosa and Staphylococcus aureus. Different LAB preparations such as untreated cell ... ...

    Abstract This study investigated the effects of lactic acid bacteria (LAB) of goat's milk origin on growth and biofilm formation of two pathogenic strains viz. Pseudomonas aeruginosa and Staphylococcus aureus. Different LAB preparations such as untreated cell free supernatants (CFSs), pH neutralized CFS (N-CFS) and heat treated CFS (H-CFS) of lactobacilli were screened for their antimicrobial and biofilm inhibitory properties. The antagonistic activities were compared to L. acidophilus, a reference probiotic strain. In addition, metabolic activity of bacterial pathogens upon CFSs challenge was also determined by MTT assay. LAB CFSs inhibited pathogen growth to a varied degree. A decrease in the number of sessile pathogen cells was observed upon exposure with untreated CFS, under both post and co-incubation strategy. The CFS of GM12, GM13, GM15 and GM17 could effectively inhibit the biofilm formation of both the pathogens to a significant level (p < 0.05) varying between 72% and 86%. Upon principal component analysis of recorded data, untreated CFS from GM12 emerged to be most promising against both the test biofilm formers. N-CFS and H-CFS preparations also limited the biofilm formation, however to a lower extent than untreated form. As also determined by MTT assay, the metabolic activity of P. aeruginosa and S. aureus were significantly reduced upon CFS exposure. The light microscopic images depicted major disruption in the biofilm matrix of pathogenic strains upon CFS challenge. Findings from this study indicate that the Lactobacillus strains of goat milk origin screened in this study may serve as bio-control agents for prophylactic use to prevent infections.
    Keywords biochemical pathways ; biofilm ; goat milk ; heat treatment ; in vitro studies ; lactic acid bacteria ; Lactobacillus ; pathogens ; pH ; principal component analysis ; probiotics ; Pseudomonas aeruginosa ; Staphylococcus aureus ; virulent strains
    Language English
    Dates of publication 2018-04
    Size p. 129-138.
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 2212-4292
    DOI 10.1016/j.fbio.2018.02.001
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Antimicrobial Resistance

    Chetan Sharma / Namita Rokana / Mudit Chandra / Brij Pal Singh / Rohini Devidas Gulhane / Jatinder Paul Singh Gill / Pallab Ray / Anil Kumar Puniya / Harsh Panwar

    Frontiers in Veterinary Science, Vol

    Its Surveillance, Impact, and Alternative Management Strategies in Dairy Animals

    2018  Volume 4

    Abstract: Antimicrobial resistance (AMR), one among the most common priority areas identified by both national and international agencies, is mushrooming as a silent pandemic. The advancement in public health care through introduction of antibiotics against ... ...

    Abstract Antimicrobial resistance (AMR), one among the most common priority areas identified by both national and international agencies, is mushrooming as a silent pandemic. The advancement in public health care through introduction of antibiotics against infectious agents is now being threatened by global development of multidrug-resistant strains. These strains are product of both continuous evolution and un-checked antimicrobial usage (AMU). Though antibiotic application in livestock has largely contributed toward health and productivity, it has also played significant role in evolution of resistant strains. Although, a significant emphasis has been given to AMR in humans, trends in animals, on other hand, are not much emphasized. Dairy farming involves surplus use of antibiotics as prophylactic and growth promoting agents. This non-therapeutic application of antibiotics, their dosage, and withdrawal period needs to be re-evaluated and rationally defined. A dairy animal also poses a serious risk of transmission of resistant strains to humans and environment. Outlining the scope of the problem is necessary for formulating and monitoring an active response to AMR. Effective and commendably connected surveillance programs at multidisciplinary level can contribute to better understand and minimize the emergence of resistance. Besides, it requires a renewed emphasis on investments into research for finding alternate, safe, cost effective, and innovative strategies, parallel to discovery of new antibiotics. Nevertheless, numerous direct or indirect novel approaches based on host–microbial interaction and molecular mechanisms of pathogens are also being developed and corroborated by researchers to combat the threat of resistance. This review places a concerted effort to club the current outline of AMU and AMR in dairy animals; ongoing global surveillance and monitoring programs; its impact at animal human interface; and strategies for combating resistance with an extensive overview on possible alternates to current day ...
    Keywords antimicrobial resistance ; antimicrobial usage ; multidrug resistance ; dairy farming ; surveillance ; alternative treatment strategies ; Veterinary medicine ; SF600-1100
    Subject code 630
    Language English
    Publishing date 2018-01-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Amelioration of Colitis in Mouse Model by Exploring Antioxidative Potentials of an Indigenous Probiotic Strain of Lactobacillus fermentum Lf1

    Ritu Chauhan / Aparna Sudhakaran Vasanthakumari / Harsh Panwar / Rashmi H. Mallapa / Raj Kumar Duary / Virender Kumar Batish / Sunita Grover

    BioMed Research International, Vol

    2014  Volume 2014

    Abstract: Based on the preliminary screening of eight indigenous putative probiotic Lactobacilli, Lactobacillus fermentum Lf1 was selected for assessing its antioxidative efficacy in DSS colitis mouse model based on its ability to enhance the expression of “Nrf2” ... ...

    Abstract Based on the preliminary screening of eight indigenous putative probiotic Lactobacilli, Lactobacillus fermentum Lf1 was selected for assessing its antioxidative efficacy in DSS colitis mouse model based on its ability to enhance the expression of “Nrf2” by 6.43-fold and malondialdehyde (MDA) inhibition by 78.1 ± 0.24% in HT-29 cells under H2O2 stress. The Disease Activity Index and histological scores of Lf1-treated mice were lower than the control group. However, expression of “Nrf2” was not observed in Lf1-treated mice. A significant increase in the expression of antioxidative enzymes such as SOD2 and TrxR-1 was recorded in both of the groups. The expression of SOD2 was significantly downregulated in colitis-induced mice by −100.00-fold relative to control group, and the downregulation was considerably reduced to −37.04-fold in colitis Lf1 treatment group. Almost, a similar trend was recorded in case of “thioredoxin” expression, though “CAT” was refractile to expression. The Lf1-treated group had decreased malondialdehyde level as compared to colitis control (37.92 ± 6.31 versus 91.13 ± 5.76 μM/g). These results point towards Lf1-induced activation of the antioxidant enzyme system in the mouse model and its prospects to be explored as a new strategy for IBD management.
    Keywords Medicine ; R
    Subject code 570
    Language English
    Publishing date 2014-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Management of metabolic syndrome through probiotic and prebiotic interventions

    Rashmi H Mallappa / Namita Rokana / Raj Kumar Duary / Harsh Panwar / Virender Kumar Batish / Sunita Grover

    Indian Journal of Endocrinology and Metabolism, Vol 16, Iss 1, Pp 20-

    2012  Volume 27

    Abstract: Metabolic syndrome is a complex disorder caused by a cluster of interrelated factors that increases the risk of cardiovascular diseases and type 2 diabetes. Obesity is the main precursor for metabolic syndrome that can be targeted in developing various ... ...

    Abstract Metabolic syndrome is a complex disorder caused by a cluster of interrelated factors that increases the risk of cardiovascular diseases and type 2 diabetes. Obesity is the main precursor for metabolic syndrome that can be targeted in developing various therapies. With this view, several physical, psychological, pharmaceutical and dietary therapies have been proposed for the management of obesity. However, dietary strategies found more appropriate without any adverse health effects. Application of probiotics and prebiotics as biotherapeutics is the new emerging area in developing dietary strategies and many people are interested in learning the facts behind these health claims. Recent studies established the role of probiotics and prebiotics in weight management with possible mechanisms of improved microbial balance, decreased food intake, decreased abdominal adiposity and increased mucosal integrity with decreased inflammatory tone. Hence, the above "Pharmaco-nutritional" approach has been selected and extensively reviewed to gain thorough knowledge on putative mechanisms of probiotic and prebiotic action in order to develop dietary strategies for the management of metabolic syndrome.
    Keywords Gut microbiota ; metabolic syndrome ; prebiotics ; probiotics ; Diseases of the endocrine glands. Clinical endocrinology ; RC648-665 ; Specialties of internal medicine ; RC581-951 ; Internal medicine ; RC31-1245 ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2012-01-01T00:00:00Z
    Publisher Endocrine Society of India
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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