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  1. Article ; Online: Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.

    Sprink, Thorben / Hartung, Frank

    International journal of molecular sciences

    2021  Volume 22, Issue 17

    Abstract: In the past, major findings in meiosis have been achieved, but questions towards the global understanding of meiosis remain concealed. In plants, one of these questions covers the need for two diverse meiotic active SPO11 proteins. ... ...

    Abstract In the past, major findings in meiosis have been achieved, but questions towards the global understanding of meiosis remain concealed. In plants, one of these questions covers the need for two diverse meiotic active SPO11 proteins. In
    MeSH term(s) Arabidopsis/chemistry ; Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Arabidopsis Proteins/physiology ; DNA Breaks, Double-Stranded ; DNA-Binding Proteins/metabolism ; Endodeoxyribonucleases/chemistry ; Endodeoxyribonucleases/genetics ; Endodeoxyribonucleases/metabolism ; Endodeoxyribonucleases/physiology ; Meiosis/physiology ; RNA Splicing/genetics ; Rad51 Recombinase/metabolism ; Recombination, Genetic ; Species Specificity
    Chemical Substances ASY1 protein, Arabidopsis ; Arabidopsis Proteins ; DNA-Binding Proteins ; ATRAD51 protein, Arabidopsis (EC 2.7.7.-) ; Rad51 Recombinase (EC 2.7.7.-) ; Endodeoxyribonucleases (EC 3.1.-) ; meiotic recombination protein SPO11 (EC 3.1.-)
    Language English
    Publishing date 2021-08-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22179346
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Genome editing around the globe: An update on policies and perceptions.

    Sprink, Thorben / Wilhelm, Ralf / Hartung, Frank

    Plant physiology

    2022  Volume 190, Issue 3, Page(s) 1579–1587

    Abstract: A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have been altered using genome editing and discussions were raised on the regulatory status of genome edited organisms esp. crops. To date, many ... ...

    Abstract A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have been altered using genome editing and discussions were raised on the regulatory status of genome edited organisms esp. crops. To date, many countries have made decisions on the regulatory status of products of genome editing, by exempting some kinds of edits from the classical GMO regulation. However, the guidance differs between countries even in the same region. Several countries are still debating the issue or are in the progress of updating guidance and regulatory systems to cover products of genome editing. The current global situation of different regulatory systems is putting a harmonized framework on genome-edited crops in the far future. In this update, we summarize the current developments in the field of regulation concerning edited crops and present a short insight into perception of genome editing in the society.
    MeSH term(s) Gene Editing ; Genome, Plant/genetics ; CRISPR-Cas Systems/genetics ; Crops, Agricultural/genetics ; Policy
    Language English
    Publishing date 2022-11-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208914-2
    ISSN 1532-2548 ; 0032-0889
    ISSN (online) 1532-2548
    ISSN 0032-0889
    DOI 10.1093/plphys/kiac359
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 1193: Show issues

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  3. Book ; Audio / Video ; Online: CRISPR/Cas9 und andere Genome Editing Techniken

    Hartung, Frank

    2017  

    Keywords ddc:630
    Language German
    Publishing date 2017-03-13
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: CRISPR/Cas9 und andere Genome Editing Techniken

    Hartung, Frank

    2017  

    Keywords Text ; abstract_or_summary ; ddc:630
    Language German
    Publishing date 2017-03-13
    Publisher Julius Kühn-Institut
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern

    Sprink, Thorben / Hartung, Frank

    2021  

    Abstract: In the past, major findings in meiosis have been achieved, but questions towards the global understanding of meiosis remain concealed. In plants, one of these questions covers the need for two diverse meiotic active SPO11 proteins. In Arabidopsis and ... ...

    Abstract In the past, major findings in meiosis have been achieved, but questions towards the global understanding of meiosis remain concealed. In plants, one of these questions covers the need for two diverse meiotic active SPO11 proteins. In Arabidopsis and other plants, both meiotic SPO11 are indispensable in a functional form for double strand break induction during meiotic prophase I. This stands in contrast to mammals and fungi, where a single SPO11 is present and sufficient. We aimed to investigate the specific function and evolution of both meiotic SPO11 paralogs in land plants. By performing immunostaining of both SPO11-1 and -2, an investigation of the spatiotemporal localization of each SPO11 during meiosis was achieved. We further exchanged SPO11-1 and -2 in Arabidopsis and could show a species-specific function of the respective SPO11. By additional changes of regions between SPO11-1 and -2, a sequence-specific function for both the SPO11 proteins was revealed. Furthermore, the previous findings about the aberrant splicing of each SPO11 were refined by narrowing them down to a specific developmental phase. These findings let us suggest that the function of both SPO11 paralogs is highly sequence specific and that the orthologs are species specific.
    Keywords Text ; ddc:630 ; meiosis -- SPO11 -- double strand breaks -- DSB -- plant -- splicing -- immunostaining
    Subject code 580
    Language English
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  6. Article ; Online: Genome editing around the globe

    Sprink, Thorben / Wilhelm, Ralf / Hartung, Frank

    An update on policies and perceptions

    2022  

    Abstract: A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have been altered using genome editing and discussions were raised on the regulatory status of genome edited organisms esp. crops. To date, many ... ...

    Abstract A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have been altered using genome editing and discussions were raised on the regulatory status of genome edited organisms esp. crops. To date, many countries have made decisions on the regulatory status of products of genome editing, by exempting some kinds of edits from the classical GMO regulation. However, the guidance differs between countries even in the same region. Several countries are still debating the issue or are in the progress of updating guidance and regulatory systems to cover products of genome editing. The current global situation of different regulatory systems is putting a harmonized framework on genome-edited crops in the far future. In this update, we summarize the current developments in the field of regulation concerning edited crops and present a short insight into perception of genome editing in the society.
    Keywords Text ; ddc:630
    Subject code 320
    Language English
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

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  7. Book ; Audio / Video ; Online: Wie CRISPR ist die Zukunft der Pflanzenzüchtung?

    Wilhelm, Ralf / Hartung, Frank / Sprink, Thorben

    2022  

    Keywords Text ; ddc:630
    Language German
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

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  8. Article ; Online: Transgene-Free Genome Editing in Plants

    Sprink, Thorben / Hartung, Frank / Metje-Sprink, Janina

    2022  

    Abstract: Genome editing has revolutionized genetics and breeding likewise. Especially in plant breeding, it opened new ways to address traits with never known specificity. In many cases genome editing tools are provided by classical transgenic methods, i.e., by ... ...

    Abstract Genome editing has revolutionized genetics and breeding likewise. Especially in plant breeding, it opened new ways to address traits with never known specificity. In many cases genome editing tools are provided by classical transgenic methods, i.e., by Agrobacterium-based delivery, but it is also possible to perform genome editing without the use of a transgene by providing proteins, or nucleic acid protein complexes. These methods have the big advantage that transgene organisms can be avoided at any time; even transgenic intermediates are not needed. However, transgene-free methods are technically challenging, and editing rates are often lower compared to classical methods. Nevertheless, it offers great opportunities to produce plants without the need of any transgene, simplifying the regulatory processes in many jurisdictions around the globe. In this chapter, we present methods and delivery methods that can be used for transgene-free editing and present first promising examples.
    Keywords Text ; ddc:630 ; Genome editing -- Mito TALEs -- Meganucleases -- Plastid editing -- Transgene-free methods
    Language English
    Publisher Springer International Publishing
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article: Genome-edited plants in the field

    Metje-Sprink, Janina / Sprink, Thorben / Hartung, Frank

    Current opinion in biotechnology. 2020 Feb., v. 61

    2020  

    Abstract: The application of site directed nucleases (SDN) for Genome Editing (GE) in plant breeding and research increases exponentially in the last few years. The main research so far was on ‘proof of concept’ studies or improvement of the precision and delivery ...

    Abstract The application of site directed nucleases (SDN) for Genome Editing (GE) in plant breeding and research increases exponentially in the last few years. The main research so far was on ‘proof of concept’ studies or improvement of the precision and delivery of the SDN. Nevertheless, a reasonable amount of research is present on market-oriented applications for cash crops such as rice but also for commercially lesser interesting crops and vegetables. Reported field trials involving GE plants are scarce around the world and almost not existing in Europe. This is due to the regulatory landscape for GE plants, which is quite distinct and especially in the European Union very demanding. By far the most field trials involve GE rice varieties in the Asian area, followed up by tomato and other vegetables and crops.
    Keywords European Union ; cash crops ; field experimentation ; gene editing ; genetically modified plants ; nucleases ; plant breeding ; rice ; tomatoes ; Europe
    Language English
    Dates of publication 2020-02
    Size p. 1-6.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2019.08.007
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3071: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Genome-edited plants in the field.

    Metje-Sprink, Janina / Sprink, Thorben / Hartung, Frank

    Current opinion in biotechnology

    2019  Volume 61, Page(s) 1–6

    Abstract: The application of site directed nucleases (SDN) for Genome Editing (GE) in plant breeding and research increases exponentially in the last few years. The main research so far was on 'proof of concept' studies or improvement of the precision and delivery ...

    Abstract The application of site directed nucleases (SDN) for Genome Editing (GE) in plant breeding and research increases exponentially in the last few years. The main research so far was on 'proof of concept' studies or improvement of the precision and delivery of the SDN. Nevertheless, a reasonable amount of research is present on market-oriented applications for cash crops such as rice but also for commercially lesser interesting crops and vegetables. Reported field trials involving GE plants are scarce around the world and almost not existing in Europe. This is due to the regulatory landscape for GE plants, which is quite distinct and especially in the European Union very demanding. By far the most field trials involve GE rice varieties in the Asian area, followed up by tomato and other vegetables and crops.
    MeSH term(s) Crops, Agricultural/genetics ; Europe ; Gene Editing ; Genome, Plant ; Plants, Genetically Modified/genetics
    Language English
    Publishing date 2019-09-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2019.08.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3071: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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