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  1. Article ; Online: Multielectrode Arrays.

    Burley, Russell / Harvey, Jenna R M

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2188, Page(s) 109–132

    Abstract: Multielectrode arrays (MEAs) are grids of substrate-integrated microelectrodes that allow for electrophysiological interrogation of dissociated cell cultures or tissue slices. Here we discuss the use of nonimplantable electrodes for studies. The methods ... ...

    Abstract Multielectrode arrays (MEAs) are grids of substrate-integrated microelectrodes that allow for electrophysiological interrogation of dissociated cell cultures or tissue slices. Here we discuss the use of nonimplantable electrodes for studies. The methods described attempt to provide a starting point for researchers new to the field who wish to begin to utilize this powerful, but daunting technology and quickly apply the basic principles to their own research interests.
    MeSH term(s) Action Potentials ; Animals ; Cell Culture Techniques/instrumentation ; Cell Culture Techniques/methods ; Electrophysiology/instrumentation ; Electrophysiology/methods ; Equipment Design ; Hippocampus/cytology ; Hippocampus/physiology ; Mice ; Microelectrodes ; Nerve Net/cytology ; Nerve Net/physiology ; Neurons/cytology ; Neurons/physiology ; Organ Culture Techniques/instrumentation ; Organ Culture Techniques/methods ; Rats ; Tissue Array Analysis/instrumentation ; Tissue Array Analysis/methods
    Language English
    Publishing date 2020-10-29
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0818-0_6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Ion Channels Controlling Circadian Rhythms in Suprachiasmatic Nucleus Excitability.

    Harvey, Jenna R M / Plante, Amber E / Meredith, Andrea L

    Physiological reviews

    2020  Volume 100, Issue 4, Page(s) 1415–1454

    Abstract: Animals synchronize to the environmental day-night cycle by means of an internal circadian clock in the brain. In mammals, this timekeeping mechanism is housed in the suprachiasmatic nucleus (SCN) of the hypothalamus and is entrained by light input from ... ...

    Abstract Animals synchronize to the environmental day-night cycle by means of an internal circadian clock in the brain. In mammals, this timekeeping mechanism is housed in the suprachiasmatic nucleus (SCN) of the hypothalamus and is entrained by light input from the retina. One output of the SCN is a neural code for circadian time, which arises from the collective activity of neurons within the SCN circuit and comprises two fundamental components:
    MeSH term(s) Animals ; CLOCK Proteins/genetics ; CLOCK Proteins/metabolism ; Circadian Rhythm/physiology ; Gene Expression Regulation ; Ion Channels/metabolism ; Neurons/physiology ; Suprachiasmatic Nucleus/physiology
    Chemical Substances Ion Channels ; CLOCK Proteins (EC 2.3.1.48)
    Language English
    Publishing date 2020-03-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 209902-0
    ISSN 1522-1210 ; 0031-9333
    ISSN (online) 1522-1210
    ISSN 0031-9333
    DOI 10.1152/physrev.00027.2019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Tau isoform-specific enhancement of L-type calcium current and augmentation of afterhyperpolarization in rat hippocampal neurons.

    Stan, Georgiana F / Church, Timothy W / Randall, Ellie / Harvey, Jenna R M / Brown, Jon T / Wilkinson, Kevin A / Hanley, Jonathan G / Marrion, Neil V

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 15231

    Abstract: Accumulation of tau is observed in dementia, with human tau displaying 6 isoforms grouped by whether they display either 3 or 4 C-terminal repeat domains (3R or 4R) and exhibit no (0N), one (1N) or two (2N) N terminal repeats. Overexpression of 4R0N-tau ... ...

    Abstract Accumulation of tau is observed in dementia, with human tau displaying 6 isoforms grouped by whether they display either 3 or 4 C-terminal repeat domains (3R or 4R) and exhibit no (0N), one (1N) or two (2N) N terminal repeats. Overexpression of 4R0N-tau in rat hippocampal slices enhanced the L-type calcium (Ca<sup>2+</sup>) current-dependent components of the medium and slow afterhyperpolarizations (AHPs). Overexpression of both 4R0N-tau and 4R2N-tau augmented Ca<sub>V</sub>1.2-mediated L-type currents when expressed in tsA-201 cells, an effect not observed with the third 4R isoform, 4R1N-tau. Current enhancement was only observed when the pore-forming subunit was co-expressed with Ca<sub>V</sub>β3 and not Ca<sub>V</sub>β2a subunits. Non-stationary noise analysis indicated that enhanced Ca<sup>2+</sup> channel current arose from a larger number of functional channels. 4R0N-tau and Ca<sub>V</sub>β3 were found to be physically associated by co-immunoprecipitation. In contrast, the 4R1N-tau isoform that did not augment expressed macroscopic L-type Ca<sup>2+</sup> current exhibited greatly reduced binding to Ca<sub>V</sub>β3. These data suggest that physical association between tau and the Ca<sub>V</sub>β3 subunit stabilises functional L-type channels in the membrane, increasing channel number and Ca<sup>2+</sup> influx. Enhancing the Ca<sup>2+</sup>-dependent component of AHPs would produce cognitive impairment that underlie those seen in the early phases of tauopathies.
    MeSH term(s) Animals ; Calcium/metabolism ; Calcium Channels, L-Type/chemistry ; Calcium Channels, L-Type/genetics ; Calcium, Dietary/metabolism ; Hippocampus/metabolism ; Humans ; Neurons/metabolism ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Rats ; Tauopathies/metabolism ; tau Proteins/genetics ; tau Proteins/metabolism
    Chemical Substances Calcium Channels, L-Type ; Calcium, Dietary ; Protein Isoforms ; tau Proteins ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2022-09-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-18648-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Discovery of

    Christie, Louisa A / Brice, Nicola L / Rowland, Anna / Dickson, Louise / Anand, Rishi / Teall, Martin / Doyle, Kevin J / Narayana, Lakshminarayana / Mitchell, Christine / Harvey, Jenna R M / Mulligan, Victoria / Dawson, Lee A / Cragg, Stephanie J / Carlton, Mark / Bürli, Roland W

    Journal of medicinal chemistry

    2023  Volume 66, Issue 17, Page(s) 11718–11731

    Abstract: Nicotinic acetylcholine receptor (nAChR) α6 subunit RNA expression is relatively restricted to midbrain regions and is located presynaptically on dopaminergic neurons projecting to the striatum. This subunit modulates dopamine neurotransmission and may ... ...

    Abstract Nicotinic acetylcholine receptor (nAChR) α6 subunit RNA expression is relatively restricted to midbrain regions and is located presynaptically on dopaminergic neurons projecting to the striatum. This subunit modulates dopamine neurotransmission and may have therapeutic potential in movement disorders. We aimed to develop potent and selective α6-containing nAChR antagonists to explore modulation of dopamine release and regulation of motor function in vivo. High-throughput screening (HTS) identified novel α6-containing nAChR antagonists and led to the development of
    MeSH term(s) Dopamine ; Brain ; Cell Membrane ; Corpus Striatum ; Nicotinic Antagonists/pharmacology ; Receptors, Nicotinic
    Chemical Substances Dopamine (VTD58H1Z2X) ; Nicotinic Antagonists ; Receptors, Nicotinic
    Language English
    Publishing date 2023-08-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218133-2
    ISSN 1520-4804 ; 0022-2623
    ISSN (online) 1520-4804
    ISSN 0022-2623
    DOI 10.1021/acs.jmedchem.3c00630
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Characterisation of C101248: A novel selective THIK-1 channel inhibitor for the modulation of microglial NLRP3-inflammasome.

    Ossola, Bernardino / Rifat, Ali / Rowland, Anna / Hunter, Helen / Drinkall, Samuel / Bender, Clare / Hamlischer, Mayida / Teall, Martin / Burley, Russell / Barker, Daneil F / Cadwalladr, David / Dickson, Louise / Lawrence, Jason M K / Harvey, Jenna R M / Lizio, Marina / Xu, Xiao / Kavanagh, Edel / Cheung, Toni / Sheardown, Steve /
    Lawrence, Catherine B / Harte, Michael / Brough, David / Madry, Christian / Matthews, Kim / Doyle, Kevin / Page, Keith / Powell, Justin / Brice, Nicola L / Bürli, Roland W / Carlton, Mark B / Dawson, Lee A

    Neuropharmacology

    2022  Volume 224, Page(s) 109330

    Abstract: Neuroinflammation, specifically the NLRP3 inflammasome cascade, is a common underlying pathological feature of many neurodegenerative diseases. Evidence suggests that NLRP3 activation involves changes in intracellular ... ...

    Abstract Neuroinflammation, specifically the NLRP3 inflammasome cascade, is a common underlying pathological feature of many neurodegenerative diseases. Evidence suggests that NLRP3 activation involves changes in intracellular K
    MeSH term(s) Animals ; Humans ; Mice ; Alzheimer Disease/metabolism ; Brain/metabolism ; Inflammasomes/metabolism ; Microglia ; Neuroinflammatory Diseases ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Potassium Channels, Tandem Pore Domain/antagonists & inhibitors
    Chemical Substances Inflammasomes ; NLR Family, Pyrin Domain-Containing 3 Protein ; Nlrp3 protein, mouse ; KCNK13 protein, human ; Kcnk13 protein, mouse ; Potassium Channels, Tandem Pore Domain
    Language English
    Publishing date 2022-11-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218272-5
    ISSN 1873-7064 ; 0028-3908
    ISSN (online) 1873-7064
    ISSN 0028-3908
    DOI 10.1016/j.neuropharm.2022.109330
    Database MEDical Literature Analysis and Retrieval System OnLINE

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