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  1. Article: In Silico and In Vitro Investigation of the Distribution and Expression of Key Genes in the Fucose Operon of

    Saif, Nehal A / Hashem, Yomna A / Amin, Heba M / Aziz, Ramy K

    Microorganisms

    2023  Volume 11, Issue 5

    Abstract: Many gut bacteria degrade polysaccharides, providing nutritional advantages to their hosts. Fucose, a mucin degradation product, was suggested as a communication molecule between the resident microbiota and external pathogens. However, the precise role ... ...

    Abstract Many gut bacteria degrade polysaccharides, providing nutritional advantages to their hosts. Fucose, a mucin degradation product, was suggested as a communication molecule between the resident microbiota and external pathogens. However, the precise role and variants of the fucose utilization pathway remain to be elucidated. Here, we computationally and experimentally investigated the fucose utilization operon of
    Language English
    Publishing date 2023-05-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11051265
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Antibiofilm activity of green synthesized silver nanoparticles against biofilm associated enterococcal urinary pathogens.

    Swidan, Nada S / Hashem, Yomna A / Elkhatib, Walid F / Yassien, Mahmoud A

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 3869

    Abstract: Biofilm-formed enterococcal urinary tract clinical isolates (n = 92) were used for studying the antibiofilm activity of cinnamon, ginger, and chemical AgNPs. The average particle sizes of cinnamon, ginger, and chemical AgNPs were 8.7, 41.98, and 55.7 nm, ...

    Abstract Biofilm-formed enterococcal urinary tract clinical isolates (n = 92) were used for studying the antibiofilm activity of cinnamon, ginger, and chemical AgNPs. The average particle sizes of cinnamon, ginger, and chemical AgNPs were 8.7, 41.98, and 55.7 nm, respectively. The results of Fourier transform infrared analysis revealed that phytocompounds, such as cinnamaldehyde and gingerol, were the main compounds incorporated in the synthesis of cinnamon and ginger AgNPs, respectively. The purity and crystalline nature of the AgNPs have been confirmed by energy dispersive X-ray and X-ray Diffraction analysis. The results of antimicrobial activity showed that MIC of ginger, cinnamon, and chemical AgNPs were 37.64, 725.7, and 61.08 μg/ml, respectively. On studying the antibiofilm activity of AgNPs at sub-MIC values (1/2, 1/4, and 1/8 MIC), the results revealed that it was concentration dependent. Therefore, further studies were carried out to evaluate the antibiofilm activity of AgNPs at a concentration of 18 μg/ml. The results showed that ginger and chemical AgNPs reduced the formed biofilm to 39.14% and 65.32% and the number of adherent cells on the urinary catheter surface to 42.73% and 69.84%, respectively, as compared to that of the control, while cinnamon AgNPs showed no significant activity. Accordingly, ginger AgNPs had the most potent antibacterial and antiadherent activity against biofilm-associated enterococcal isolates.
    MeSH term(s) Anti-Bacterial Agents/chemistry ; Biofilms ; Cinnamomum zeylanicum ; Enterococcus ; Zingiber officinale ; Metal Nanoparticles/chemistry ; Microbial Sensitivity Tests ; Plant Extracts/chemistry ; Silver/chemistry ; Silver/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Plant Extracts ; Silver (3M4G523W1G)
    Language English
    Publishing date 2022-03-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-07831-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Phenotype-Genotype Correlations and Distribution of Key Virulence Factors in

    Hashem, Yomna A / Abdelrahman, Khaled A / Aziz, Ramy K

    Infection and drug resistance

    2021  Volume 14, Page(s) 1713–1723

    Abstract: Background and objective: Enterococcus faecalis: Methods: We phenotypically analyzed 60 urinary isolates, identified as : Results: All isolates formed biofilms, mostly with strong to moderate ability. Although : Conclusion: A clear variability ... ...

    Abstract Background and objective: Enterococcus faecalis
    Methods: We phenotypically analyzed 60 urinary isolates, identified as
    Results: All isolates formed biofilms, mostly with strong to moderate ability. Although
    Conclusion: A clear variability in virulence gene distribution among the isolates was observed, and no particular pattern was associated with UTI. Whereas all isolates carried at least
    Language English
    Publishing date 2021-05-10
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2494856-1
    ISSN 1178-6973
    ISSN 1178-6973
    DOI 10.2147/IDR.S305167
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Rosemary and neem: an insight into their combined anti-dandruff and anti-hair loss efficacy.

    Hashem, Mona M / Attia, Dalia / Hashem, Yomna A / Hendy, Moataz S / AbdelBasset, Safa / Adel, Farah / Salama, Maha M

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 7780

    Abstract: Dandruff, a common scalp disorder characterized by flaking dead skin, is often treated with conventional topical products. However, limitations exist due to potential side effects and high costs. Therefore, searching for natural, cost-effective solutions ...

    Abstract Dandruff, a common scalp disorder characterized by flaking dead skin, is often treated with conventional topical products. However, limitations exist due to potential side effects and high costs. Therefore, searching for natural, cost-effective solutions for dandruff and hair loss is crucial. Rosemary herb and neem tree, both cultivated in Egypt, possess well-documented anti-inflammatory properties derived from their rich phenolic phytoconstituents. This study formulated a standardized combined extract of rosemary and neem (RN-E 2:1) into hair gel and leave-in tonic formats. This extract demonstrated superior efficacy against Malassezia furfur (a causative agent of dandruff) and Trichophyton rubrum (associated with scalp disorders) compared to the conventional antifungal agent, ketoconazole. The combined extract (RN-E 2:1) also exhibited potent anti-inflammatory activity. Additionally, the suppression of iNOS expression is considered concentration-dependent. Quality control verified formulation stability, and ex-vivo studies confirmed effective ingredient penetration into the epidermis, the primary site of fungal presence. Remarkably, both formulations outperformed the standard treatment, minoxidil in hair growth trials. These findings highlight the potential of natural extracts for scalp and hair health.
    MeSH term(s) Dandruff/drug therapy ; Dandruff/microbiology ; Rosmarinus ; Azadirachta ; Alopecia/drug therapy ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use
    Chemical Substances Plant Extracts ; Anti-Inflammatory Agents
    Language English
    Publishing date 2024-04-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-57838-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Comparative metabolite profiling of four polyphenol rich Morus leaves extracts in relation to their antibiofilm activity against Enterococcus faecalis.

    Salem, Mohamed A / Salama, Maha M / Ezzat, Shahira M / Hashem, Yomna A

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 20168

    Abstract: Enterococci are a common cause of urinary tract infections. The severity of enterococcal infections is associated with their ability to form biofilms. Morus leaves are known as a natural antibacterial, however, their antibiofilm activity against ... ...

    Abstract Enterococci are a common cause of urinary tract infections. The severity of enterococcal infections is associated with their ability to form biofilms. Morus leaves are known as a natural antibacterial, however, their antibiofilm activity against Enterococcus remains unveiled. This study aimed to evaluate the ability of four polyphenol-rich Morus leaves extracts (Morus nigra, M. rubra, M. macroura, and M. alba) to inhibit biofilm formed by enterococcal clinical isolates in relation to their metabolic profiling. Results revealed that 48% of the isolates formed strong biofilm, 28% formed moderate biofilm, 20% formed weak biofilm, and only 4% did not form a biofilm. The strong biofilm-forming isolates were E. faecalis, and hence were chosen for this study. The antibiofilm activity of the four polyphenol-rich Morus leaves extracts revealed that the M. nigra extract exhibited the highest percentage of biofilm inhibition followed by M. rubra then M. macroura and the least inhibition was detected in M. alba, and these results were in accordance with the phenolic and flavonoid contents of each extract. UPLC-ESI-MS/MS identified 61 polyphenolic compounds in the four extracts. Further, multivariate analysis confirmed clear segregation of M. nigra from the other species suggesting disparity in its metabolome, with accumulation of flavonoids, anthocyanidins, phenolic acids and coumarin derivatives. Quercetin and kaempferol glycosides were found to be positively and significantly correlated to the antibiofilm activity. In conclusion, M. nigra ethanolic extracts showed the highest phenolic content and antibiofilm activity and they could be developed as a complementary treatment for the development of antimicrobial agents.
    MeSH term(s) Morus ; Polyphenols/pharmacology ; Enterococcus faecalis ; Tandem Mass Spectrometry ; Plant Extracts/pharmacology ; Flavonoids/pharmacology ; Flavonoids/analysis ; Phenols/pharmacology ; Biofilms
    Chemical Substances Polyphenols ; Plant Extracts ; Flavonoids ; Phenols
    Language English
    Publishing date 2022-11-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-24382-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Biofilm formation in enterococci: genotype-phenotype correlations and inhibition by vancomycin.

    Hashem, Yomna A / Amin, Heba M / Essam, Tamer M / Yassin, Aymen S / Aziz, Ramy K

    Scientific reports

    2017  Volume 7, Issue 1, Page(s) 5733

    Abstract: Enterococci are nosocomial pathogens that can form biofilms, which contribute to their virulence and antibiotic resistance. Although many genes involved in biofilm formation have been defined, their distribution among enterococci has not been ... ...

    Abstract Enterococci are nosocomial pathogens that can form biofilms, which contribute to their virulence and antibiotic resistance. Although many genes involved in biofilm formation have been defined, their distribution among enterococci has not been comprehensively studied on a genome scale, and their diagnostic ability to predict biofilm phenotypes is not fully established. Here, we assessed the biofilm-forming ability of 90 enterococcal clinical isolates. Major patterns of virulence gene distribution in enterococcal genomes were identified, and the differentiating virulence genes were screened by polymerase chain reaction (PCR) in 31 of the clinical isolates. We found that detection of gelE in Enterococcus faecalis is not sufficient to predict gelatinase activity unless fsrAB, or fsrB alone, is PCR-positive (P = 0.0026 and 0.0012, respectively). We also found that agg is significantly enriched in isolates with medium and strong biofilm formation ability (P = 0.0026). Additionally, vancomycin, applied at sub minimal inhibitory concentrations, inhibited biofilm in four out of five strong biofilm-forming isolates. In conclusion, we suggest using agg and fsrB genes, together with the previously established gelE, for better prediction of biofilm strength and gelatinase activity, respectively. Future studies should explore the mechanism of biofilm inhibition by vancomycin and its possible use for antivirulence therapy.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Biofilms/drug effects ; Biofilms/growth & development ; Egypt ; Enterococcus/drug effects ; Enterococcus/genetics ; Enterococcus/isolation & purification ; Enterococcus/physiology ; Genes, Bacterial ; Genetic Association Studies ; Genotype ; Gram-Positive Bacterial Infections/microbiology ; Hospitals ; Humans ; Polymerase Chain Reaction ; Vancomycin/pharmacology ; Virulence Factors/genetics
    Chemical Substances Anti-Bacterial Agents ; Virulence Factors ; Vancomycin (6Q205EH1VU)
    Language English
    Publishing date 2017-07-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-05901-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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