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Article ; Online: Late Occurrence of the Tubo-Ovarian Abscess after Appendectomy for Perforated Appendicitis in a Virginal Adolescent Girl.

Nishida, Nanako / Shono, Takeshi / Shono, Kumiko / Hashimoto, Yoshiko / Kawakami, Kosuke

Journal of pediatric and adolescent gynecology

2022 Volume 35, Issue 4, Page(s) 509–511

Abstract: Background: Tubo-ovarian abscess (TOA) is generally seen in sexually active women. It is rarely reported in virginal adolescent girls.: Case: A 12-year-old virginal girl was referred to us for repeated fever and right lower abdominal pain. She had ... ...

Abstract	Background: Tubo-ovarian abscess (TOA) is generally seen in sexually active women. It is rarely reported in virginal adolescent girls. Case: A 12-year-old virginal girl was referred to us for repeated fever and right lower abdominal pain. She had undergone an appendectomy for a perforated appendix with abscess 5 years previously. Laparoscopic surgery revealed pelvic adhesions associated with TOA in the right pelvis. At 2 months after laparoscopic drainage, she underwent resection of the affected tube with wedge resection of the ipsilateral ovary due to the recurrence of TOA. Summary and conclusion: Late occurrence of TOA should be considered in the differential diagnosis of repeated abdominal pain and fever in virginal adolescent girls with a history of appendectomy for complicated appendicitis, even if the history is remote.
MeSH term(s)	Abdominal Abscess/etiology ; Abdominal Abscess/surgery ; Abdominal Pain/etiology ; Abscess/complications ; Abscess/surgery ; Adolescent ; Appendectomy ; Appendicitis/complications ; Appendicitis/surgery ; Child ; Female ; Humans ; Salpingitis
Language	English
Publishing date	2022-01-14
Publishing country	United States
Document type	Case Reports
ZDB-ID	1325079-6
ISSN	1873-4332 ; 1083-3188
ISSN (online)	1873-4332
ISSN	1083-3188
DOI	10.1016/j.jpag.2022.01.001
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Zs.A 2337: Show issues

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Article ; Online: Infantile urethrovaginal reflux in three cases.

Sakakura, Hikari / Shono, Kumiko / Hashimoto, Yoshiko / Shono, Takeshi

Pediatrics international : official journal of the Japan Pediatric Society

2021 Volume 64, Issue 1, Page(s) e14736

MeSH term(s)	Humans ; Infant ; Male ; Ultrasonography ; Urethra/diagnostic imaging ; Urinary Tract Infections ; Vesico-Ureteral Reflux/diagnosis
Language	English
Publishing date	2021-10-17
Publishing country	Australia
Document type	Journal Article
ZDB-ID	1470376-2
ISSN	1442-200X ; 1328-8067
ISSN (online)	1442-200X
ISSN	1328-8067
DOI	10.1111/ped.14736
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Zs.A 848: Show issues

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Article: Inflammatory Cytokine-Induced HIF-1 Activation Promotes Epithelial-Mesenchymal Transition in Endometrial Epithelial Cells.

Hashimoto, Yoshiko / Tsuzuki-Nakao, Tomoko / Kida, Naoko / Matsuo, Yoshiyuki / Maruyama, Tetsuo / Okada, Hidetaka / Hirota, Kiichi

Biomedicines

2023 Volume 11, Issue 1

Abstract: The endometrium undergoes repeated proliferation and shedding during the menstrual cycle. Significant changes to this environment include fluctuations in the partial pressure of oxygen, exposure to a high-cytokine environment associated with intrauterine ...

Abstract	The endometrium undergoes repeated proliferation and shedding during the menstrual cycle. Significant changes to this environment include fluctuations in the partial pressure of oxygen, exposure to a high-cytokine environment associated with intrauterine infection, and inflammation. Chronic endometritis is a condition wherein mild inflammation persists in the endometrium and is one of the causes of implantation failure and miscarriage in early pregnancy. It is thought that the invasion of embryos into the endometrium requires epithelial-mesenchymal transition (EMT)-associated changes in the endometrial epithelium. However, the effects of inflammation on the endometrium remain poorly understood. In this study, we investigated the effects of the intrauterine oxygen environment, hypoxia-inducible factor (HIF), and inflammation on the differentiation and function of endometrial epithelial cells. We elucidated the ways in which inflammatory cytokines affect HIF activity and EMT in an immortalized cell line (EM-E6/E7/TERT) derived from endometrial epithelium. Pro-inflammatory cytokines caused significant accumulation of HIF-1α protein, increased HIF-1α mRNA levels, and enhanced hypoxia-induced accumulation of HIF-1α protein. The combined effect of inflammatory cytokines and hypoxia increased the expression of EMT-inducing factors and upregulated cell migration. Our findings indicate that pro-inflammatory factors, including cytokines and LPS, work synergistically with hypoxia to activate HIF-1 and promote EMT in endometrial epithelial cells.
Language	English
Publishing date	2023-01-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines11010210
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Article: Emergent laparoscopic dome resection and omental suturing to the splenic parenchymal edge for a spontaneously ruptured non-parasitic large splenic cyst in a pediatric patient: a case report.

Shono, Kumiko / Hashimoto, Yoshiko / Shono, Takeshi

Surgical case reports

2019 Volume 5, Issue 1, Page(s) 201

Abstract: Background: Spontaneously ruptured large splenic cyst is a rare in children, and traditionally total or partial splenectomy has been performed for treating an emergent case. We herein present a first case with spontaneously ruptured pediatric splenic ... ...

Abstract	Background: Spontaneously ruptured large splenic cyst is a rare in children, and traditionally total or partial splenectomy has been performed for treating an emergent case. We herein present a first case with spontaneously ruptured pediatric splenic cyst treated with emergent laparoscopic dome resection with omental suturing to the parenchymal edge of the spleen. Case presentation: A 12-year-old girl with a spontaneously ruptured large non-parasitic splenic cyst (SC) was successfully treated by emergent laparoscopic dome resection with omental suturing to the edge of the splenic parenchyma. The patient presented with acute abdominal pain and was diagnosed with a ruptured non-parasitic SC and peritonitis by contrast-enhanced computed tomography (CT). Emergent laparoscopic dome resection of the SC and omental suturing to the splenic parenchymal edge were then performed. The protruding part of the cyst wall was completely resected using an ultrasonically activated device (USAD), and the greater omentum was then sutured to the anterior edge of the splenic parenchyma under a laparoscopic view. No complications were observed during the operation. A histological examination revealed a congenital splenic cyst lined by epithelial cells. The postoperative course was uneventful, and an ultrasound scan showed no evidence of cyst recurrence at 3 years after the operation. Conclusions: This minimally invasive laparoscopic procedure was feasible and effective for treating a ruptured large splenic cyst in an emergent pediatric patient.
Language	English
Publishing date	2019-12-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2809613-7
ISSN	2198-7793
ISSN	2198-7793
DOI	10.1186/s40792-019-0750-2
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Article ; Online: Laparoscopic Abdominoscrortal Hydrocele: A Case Series.

Funatsu, Yasutaka / Shono, Kumiko / Hashimoto, Yoshiko / Shirai, Takeshi / Shono, Takeshi

Urology

2020 Volume 145, Page(s) 236–242

Abstract: Objective: To evaluate the effect of laparoscopic percutaneous extraperitoneal closure (LPEC) of the internal inguinal ring for the treatment in pediatric abdominoscrotal hydrocele (ASH) and to assess the feasibility and safety of the procedures.: ... ...

Abstract	Objective: To evaluate the effect of laparoscopic percutaneous extraperitoneal closure (LPEC) of the internal inguinal ring for the treatment in pediatric abdominoscrotal hydrocele (ASH) and to assess the feasibility and safety of the procedures. Patients and methods: Data were collected from the charts of patients with ASH who underwent surgery in Kokura Medical Center from April 2014 to December 2019. The patients' characteristics, preoperative diagnosis, forms of abdominal components, presence of patent processus vaginalis (PPV), associated pathologies, and postoperative results were evaluated. Results: The study population included 10 patients (4.3% of all 230 hydroceles). The mean age of 10 patients was 3.5 years (range, 7 months to 7 years). A preoperative diagnosis of ASH was made in 3 patients. In the other 7 patients, ASH was detected during laparoscopic repair of the scrotal hydrocele. The abdominal forms of hydrocele were monolocular cysts (n = 6) and multilocular cysts (n = 4). PPV was detected by laparoscopy in all cases. Six patients had contralateral pathologies, including PPV (n = 4), inguinal hernia (n = 1), and scrotal hydrocele (n = 1). One patient had ipsilateral undescended testis. Preoperative ultrasonography showed some degree of testicular dysmorphism on the affected side in 4 cases. In all cases, treatment was accomplished by closing the PPV at the internal inguinal ring by LPEC procedures. No patients had postoperative complications, including recurrent ASH or hydrocele after ASH repair (mean follow-up, 2.6 years). Conclusion: LPEC may be an adequate and minimally invasive method for the treatment of the pediatric ASH.
MeSH term(s)	Child ; Child, Preschool ; Feasibility Studies ; Humans ; Infant ; Laparoscopy/adverse effects ; Male ; Testicular Hydrocele/surgery ; Urologic Surgical Procedures, Male/adverse effects ; Urologic Surgical Procedures, Male/methods
Language	English
Publishing date	2020-07-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	192062-5
ISSN	1527-9995 ; 0090-4295
ISSN (online)	1527-9995
ISSN	0090-4295
DOI	10.1016/j.urology.2020.07.032
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Zs.A 1142: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Transcriptional regulation of LGALS9 by HAND2 and FOXO1 in human endometrial stromal cells in women with regular cycles.

Murata, Hiromi / Tanaka, Susumu / Hisamatsu, Yoji / Tsubokura, Hiroaki / Hashimoto, Yoshiko / Kitada, Masaaki / Okada, Hidetaka

Molecular human reproduction

2021 Volume 27, Issue 11

Abstract: Uterine natural killer cells are regulated via surface inhibitory receptors for IL15 and galectin-9 (LGALS9) secreted by endometrial stromal cells (ESCs). However, the mechanism that regulates LGALS9 mRNA levels in ESCs is unclear. The aim of this study ... ...

Abstract	Uterine natural killer cells are regulated via surface inhibitory receptors for IL15 and galectin-9 (LGALS9) secreted by endometrial stromal cells (ESCs). However, the mechanism that regulates LGALS9 mRNA levels in ESCs is unclear. The aim of this study is to clarify the transcriptional regulation of LGALS9 in ESCs. Here, LGALS9 mRNA expression levels significantly decreased in the endometrial tissue in the early- to mid-secretory phase, and recovered in the mid- to late-secretory phase, compared to that in the proliferative phase. In ESCs, LGALS9 mRNA expression significantly decreased following estradiol + medroxyprogesterone acetate treatment for 1 day and increased after 12 days compared to that in the control. The transcriptional activity of the LGALS9 upstream region was upregulated by heart and neural crest derivatives expressed 2 (HAND2) and downregulated by forkhead box O1 (FOXO1). In ESCs, HAND2 expression significantly increased throughout the 12 days treatment with steroid hormones, whereas FOXO1 expression significantly increased on Day 1, reached a plateau, and significantly increased again after 6 days of treatment. Levels of FOXO1 phosphorylation (pFOXO1) remained unchanged after a 3-day treatment of ESCs with steroid hormones, but significantly increased following a 12-day treatment. pFOXO1 could not bind to the DNA and was thus unable to directly suppress LGALS9 transcription. Therefore, expression level of HAND2 and phosphorylation status of FOXO1 may determine LGALS9 mRNA expression. This study provides a novel molecular mechanism underlying the transcriptional regulation of LGALS9 mRNA in ESCs, which could be valuable in the treatment of diseases associated with decidualization failure.
MeSH term(s)	Adult ; Basic Helix-Loop-Helix Transcription Factors/genetics ; Basic Helix-Loop-Helix Transcription Factors/metabolism ; Endometrium/drug effects ; Endometrium/metabolism ; Estradiol/pharmacology ; Female ; Forkhead Box Protein O1/genetics ; Forkhead Box Protein O1/metabolism ; Galectins/genetics ; Galectins/metabolism ; Humans ; Medroxyprogesterone Acetate/pharmacology ; Menstrual Cycle/drug effects ; Menstrual Cycle/genetics ; Menstrual Cycle/metabolism ; Middle Aged ; Phosphorylation ; Stromal Cells/drug effects ; Stromal Cells/metabolism ; Transcription, Genetic/drug effects
Chemical Substances	Basic Helix-Loop-Helix Transcription Factors ; FOXO1 protein, human ; Forkhead Box Protein O1 ; Galectins ; HAND2 protein, human ; LGALS9 protein, human ; Estradiol (4TI98Z838E) ; Medroxyprogesterone Acetate (C2QI4IOI2G)
Language	English
Publishing date	2021-09-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1324348-2
ISSN	1460-2407 ; 1360-9947
ISSN (online)	1460-2407
ISSN	1360-9947
DOI	10.1093/molehr/gaab063
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Zs.A 4482: Show issues

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Article ; Online: Matrix Metalloproteinases in Human Decidualized Endometrial Stromal Cells.

Hisamatsu, Yoji / Murata, Hiromi / Tsubokura, Hiroaki / Hashimoto, Yoshiko / Kitada, Masaaki / Tanaka, Susumu / Okada, Hidetaka

Current issues in molecular biology

2021 Volume 43, Issue 3, Page(s) 2111–2123

Abstract: Cyclic changes, such as growth, decidualization, shedding, and regeneration, in the human endometrium are regulated by the reciprocal action of female hormones, such as estradiol ( ... ...

Abstract	Cyclic changes, such as growth, decidualization, shedding, and regeneration, in the human endometrium are regulated by the reciprocal action of female hormones, such as estradiol (E
MeSH term(s)	Adult ; Biomarkers ; Cells, Cultured ; Decidua/cytology ; Decidua/metabolism ; Endometrium/cytology ; Endometrium/metabolism ; Female ; Gene Expression Regulation/drug effects ; Humans ; Matrix Metalloproteinases/genetics ; Matrix Metalloproteinases/metabolism ; Middle Aged ; Steroids/metabolism ; Steroids/pharmacology ; Stromal Cells/drug effects ; Stromal Cells/metabolism ; Tissue Inhibitor of Metalloproteinases/metabolism ; Young Adult
Chemical Substances	Biomarkers ; Steroids ; Tissue Inhibitor of Metalloproteinases ; Matrix Metalloproteinases (EC 3.4.24.-)
Language	English
Publishing date	2021-11-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2000024-8
ISSN	1467-3045 ; 1467-3037
ISSN (online)	1467-3045
ISSN	1467-3037
DOI	10.3390/cimb43030146
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Zs.A 5122: Show issues

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Article: NDRG1 is expressed in human granulosa cells: An implicative role of NDRG1 in the ovary.

Nishigaki, Akemi / Tsubokura, Hiroaki / Ishida, Mitsuaki / Hashimoto, Yoshiko / Yoshida, Aya / Hisamatsu, Yoji / Tsuzuki-Nakao, Tomoko / Murata, Hiromi / Okada, Hidetaka

Reproductive medicine and biology

2022 Volume 21, Issue 1, Page(s) e12437

Abstract: Purpose: N-myc downstream-regulated gene 1 (NDRG1) is expressed in various human tissues and plays a role in regulating cellular proliferation, angiogenesis, and hypoxia sensing. However, the role of NDRG1 in the ovary remains poorly understood. ... ...

Abstract	Purpose: N-myc downstream-regulated gene 1 (NDRG1) is expressed in various human tissues and plays a role in regulating cellular proliferation, angiogenesis, and hypoxia sensing. However, the role of NDRG1 in the ovary remains poorly understood. Therefore, we investigated NDRG1 expression and the role of NDRG1 in the human ovary. Methods: Follicular fluid (FF) and luteinized granulosa cells were collected from follicles during oocyte retrieval. KGN cells were cultured with cobalt chloride (CoCl Results: NDRG1 protein was expressed in luteinized granulosa cells. NDRG1 concentration was positively correlated with vascular endothelial growth factor (VEGF) and progesterone concentrations in FF. CoCl Conclusions: These results indicate that ovarian NDRG1 may play important roles in follicular development, especially in the early luteinization of pre-ovulatory follicles.
Language	English
Publishing date	2022-01-05
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2185775-1
ISSN	1447-0578 ; 1445-5781
ISSN (online)	1447-0578
ISSN	1445-5781
DOI	10.1002/rmb2.12437
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Zs.A 6075: Show issues

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Article: Congenital hyperinsulinism associated with Hirschsprung's disease-a report of an extremely rare case.

Shono, Takeshi / Shono, Kumiko / Hashimoto, Yoshiko / Taguchi, Shohei / Masuda, Masanori / Muramori, Kastumi / Taguchi, Tomoaki

Surgical case reports

2020 Volume 6, Issue 1, Page(s) 4

Abstract: Background: Congenital hyperinsulinism (CH) is a rare disease, characterized by severe hypoglycemia induced by inappropriate insulin secretion from pancreatic beta-cells in neonate and infant. Hirschsprung's disease (HD) is also a rare disease in which ... ...

Abstract	Background: Congenital hyperinsulinism (CH) is a rare disease, characterized by severe hypoglycemia induced by inappropriate insulin secretion from pancreatic beta-cells in neonate and infant. Hirschsprung's disease (HD) is also a rare disease in which infants show severe bowel movement disorder. We herein report an extremely rare case of combined CH and HD. Case presentation: The patient was a full-term male infant who showed poor feeding, vomiting, and hypotonia with lethargy on the day of birth. He was transferred to tertiary hospital after a laboratory analysis revealed hyperinsulinemic hypoglycemia. The patient showed remarkable abdominal distension without meconium defecation. An abdominal X-ray showed marked dilatation of the large bowel. He was diagnosed with CH (nesidioblastosis) associated with suspected HD. He was initially treated with an intravenous infusion of high-dose glucose with the intermittent injection of glucagon. This was successfully followed by treatment with diazoxide and octreotide (a somatostatin analog). At 8 months of age, HD was confirmed by the acetylcholinesterase staining of a rectal mucosal biopsy specimen, and a transanal pull-through operation was performed to treat HD. At 14 months of age, subtotal pancreatectomy was performed for the treatment of focal CH located in the pancreatic body. His postoperative course over the past 12 years has been uneventful without any neurologic or bowel movement disorders. Conclusions: Although it is extremely rare for CH to be associated with HD, associated HD should be considered when a patient with CH presents severe constipation.
Language	English
Publishing date	2020-01-08
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2809613-7
ISSN	2198-7793
ISSN	2198-7793
DOI	10.1186/s40792-020-0778-3
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Article: Utility of Positional Instillation of Contrast Cystography for Diagnosing Occult Vesicoureteral Reflux in Children: A Report of Two Cases.

Sato, Rie / Shono, Takeshi / Nakashima, Yasutaka / Shono, Kumiko / Hashimoto, Yoshiko / Mori, Sayo / Yasunaga, Yukie / Ogata, Reina / Yamaguchi, Kenichiro / Watanabe, Kyoko / Yamashita, Hironori

Journal of UOEH

2023 Volume 45, Issue 1, Page(s) 9–14

Abstract: Positional instillation of contrast (PIC) cystography is effective for detecting occult vesicoureteral reflux (VUR), which can not be revealed by standard voiding cystourethrography (VCUG). We experienced two cases of young female patients; one had ... ...

Abstract	Positional instillation of contrast (PIC) cystography is effective for detecting occult vesicoureteral reflux (VUR), which can not be revealed by standard voiding cystourethrography (VCUG). We experienced two cases of young female patients; one had repeated urinary tract infection with a negative VUR on standard VCUG, and the other had findings suggestive of reflux hydronephrosis and intolerance of standard VCUG. They underwent PIC cystography, and occult VUR was detected in both cases. Both were successfully treated with simultaneous endoscopic injection therapy with dextranomer/hyaluronic acid. PIC cystography is useful for detecting occult VUR in children with negative VUR findings on standard VCUG or who are unable to tolerate standard VCUG.
MeSH term(s)	Humans ; Child ; Female ; Cystography ; Vesico-Ureteral Reflux/diagnostic imaging ; Combined Modality Therapy
Language	English
Publishing date	2023-03-06
Publishing country	Japan
Document type	Case Reports ; Journal Article
ZDB-ID	632724-2
ISSN	2187-2864 ; 0387-821X
ISSN (online)	2187-2864
ISSN	0387-821X
DOI	10.7888/juoeh.45.9
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