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  1. Article: A Rapid Seedling Resistance Assay Identifies Wild Tomato Lines That Are Resistant to Pseudomonas syringae pv. tomato Race 1.

    Hassan, J A / Zhou, Y J / Lewis, J D

    Molecular plant-microbe interactions : MPMI

    2017  Volume 30, Issue 9, Page(s) 701–709

    Abstract: Bacterial speck caused by Pseudomonas syringae has historically been controlled by the Pto/Prf gene cluster. Emerging strains like P. syringae pv. tomato race 1 overcome resistance conferred by Pto/Prf, and can cause serious crop loss under appropriate ... ...

    Abstract Bacterial speck caused by Pseudomonas syringae has historically been controlled by the Pto/Prf gene cluster. Emerging strains like P. syringae pv. tomato race 1 overcome resistance conferred by Pto/Prf, and can cause serious crop loss under appropriate environmental conditions. We developed a rapid assay to screen wild tomato seedlings for resistance to P. syringae pv. tomato race 1. We established the seedling resistance assay using the well-characterized P. syringae pv. tomato race 0 strain, DC3000, which is recognized in tomato cultivars carrying Pto/Prf (PtoR) and causes disease in isogenic lines lacking this cluster (PtoS). We optimized infectious conditions for P. syringae on tomato seedlings and demonstrated that tomato seedlings respond like adult tomato plants in critical measures of susceptibility and immunity, including the hypersensitive response, rapid ion leakage, restricted bacterial proliferation, and phenotypic resistance. After establishing infectious conditions for P. syringae pv. tomato race 1 on tomato seedlings, we screened 96 wild accessions and identified two accessions with strong P. syringae pv. tomato race 1 resistance, Solanum neorickii LA1329 and S. habrochaites LA1253, which are also resistant to bacterial infection as adult plants. This rapid high throughput seedling assay has many advantages, including reduced plant growth time and large sample sizes, and will allow for large-scale screening of resistance in tomato.
    Language English
    Publishing date 2017-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 743331-1
    ISSN 1943-7706 ; 0894-0282
    ISSN (online) 1943-7706
    ISSN 0894-0282
    DOI 10.1094/MPMI-11-16-0247-R
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A Rapid Seedling Resistance Assay Identifies Wild Tomato Lines That Are Resistant to Pseudomonas syringae pv. tomato Race 1

    Hassan, J. A. / Zhou, Y. J. / Lewis, J. D.

    Molecular Plant-Microbe Interactions. 2017 Sept., v. 30, no. 9 p.701-709

    2017  

    Abstract: Bacterial speck caused by Pseudomonas syringae has historically been controlled by the Pto/Prf gene cluster. Emerging strains like P. syringae pv. tomato race 1 overcome resistance conferred by Pto/Prf, and can cause serious crop loss under appropriate ... ...

    Abstract Bacterial speck caused by Pseudomonas syringae has historically been controlled by the Pto/Prf gene cluster. Emerging strains like P. syringae pv. tomato race 1 overcome resistance conferred by Pto/Prf, and can cause serious crop loss under appropriate environmental conditions. We developed a rapid assay to screen wild tomato seedlings for resistance to P. syringae pv. tomato race 1. We established the seedling resistance assay using the well-characterized P. syringae pv. tomato race 0 strain, DC3000, which is recognized in tomato cultivars carrying Pto/Prf (PtoR) and causes disease in isogenic lines lacking this cluster (PtoS). We optimized infectious conditions for P. syringae on tomato seedlings and demonstrated that tomato seedlings respond like adult tomato plants in critical measures of susceptibility and immunity, including the hypersensitive response, rapid ion leakage, restricted bacterial proliferation, and phenotypic resistance. After establishing infectious conditions for P. syringae pv. tomato race 1 on tomato seedlings, we screened 96 wild accessions and identified two accessions with strong P. syringae pv. tomato race 1 resistance, Solanum neorickii LA1329 and S. habrochaites LA1253, which are also resistant to bacterial infection as adult plants. This rapid high throughput seedling assay has many advantages, including reduced plant growth time and large sample sizes, and will allow for large-scale screening of resistance in tomato.
    Keywords Pseudomonas syringae pv. tomato ; Solanum neorickii ; adults ; bacterial infections ; bacterial speck ; crop losses ; cultivars ; hypersensitive response ; immunity ; multigene family ; phenotype ; plant growth ; seedlings ; tomatoes
    Language English
    Dates of publication 2017-09
    Size p. 701-709.
    Publishing place Molecular Plant-Microbe Interactions
    Document type Article ; Online
    ZDB-ID 743331-1
    ISSN 1943-7706 ; 0894-0282
    ISSN (online) 1943-7706
    ISSN 0894-0282
    DOI 10.1094/MPMI-11-16-0247-R
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: The triad of lichen planus, thymoma and liver cirrhosis-hepatoma. First reported case.

    Hassan, J A / Saadiah, S / Roslina, A M / Atan, M / Masir, N / Hussein, S / Ganesapillai, T

    The Malaysian journal of medical sciences : MJMS

    2012  Volume 7, Issue 2, Page(s) 38–42

    Abstract: We describe a patient with liver cirrhosis who presented with erosive oral and cutaneous lichen planus (LP) and incidentally was found simultaneously to have thymoma and hepatoma. We support the notion forwarded earlier that LP and chronic liver disease ... ...

    Abstract We describe a patient with liver cirrhosis who presented with erosive oral and cutaneous lichen planus (LP) and incidentally was found simultaneously to have thymoma and hepatoma. We support the notion forwarded earlier that LP and chronic liver disease is more than a mere coincidence and that there is a non-coincidental association between LP and thymoma. We believe this is also the first reported case in the English Literature of coexistence of the three condition LP, thymoma and hepatoma complicating liver disease.
    Language English
    Publishing date 2012-06-13
    Publishing country Malaysia
    Document type Case Reports
    ZDB-ID 2197205-9
    ISSN 2180-4303 ; 1394-195X
    ISSN (online) 2180-4303
    ISSN 1394-195X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Henna causes life threatening haemolysis in glucose-6-phosphate dehydrogenase deficiency.

    Raupp, P / Hassan, J A / Varughese, M / Kristiansson, B

    Archives of disease in childhood

    2001  Volume 85, Issue 5, Page(s) 411–412

    Abstract: Haemolytic crisis in glucose-6-phosphate dehydrogenase deficient individuals following topical application of henna occurred in four children: a female neonate (haemoglobin 50 g/l, serum bilirubin 700 micromol/l), who recovered after exchange transfusion; ...

    Abstract Haemolytic crisis in glucose-6-phosphate dehydrogenase deficient individuals following topical application of henna occurred in four children: a female neonate (haemoglobin 50 g/l, serum bilirubin 700 micromol/l), who recovered after exchange transfusion; a male infant (haemoglobin 28 g/l) who died despite transfusion; and two preschool children (haemoglobin 40 and 41 g/l respectively).
    MeSH term(s) Acute Disease ; Child, Preschool ; Coloring Agents/adverse effects ; Fatal Outcome ; Female ; Glycogen Storage Disease Type I/blood ; Hemolysis/drug effects ; Humans ; Infant ; Infant, Newborn ; Male ; Naphthoquinones/adverse effects
    Chemical Substances Coloring Agents ; Naphthoquinones ; lawsone (TLH4A6LV1W)
    Language English
    Publishing date 2001-02-06
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 524-1
    ISSN 1468-2044 ; 0003-9888 ; 1359-2998
    ISSN (online) 1468-2044
    ISSN 0003-9888 ; 1359-2998
    DOI 10.1136/adc.85.5.411
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Bronchodilator response to inhaled beta-2 agonist and anticholinergic drugs in patients with bronchiectasis.

    Hassan, J A / Saadiah, S / Roslan, H / Zainudin, B M

    Respirology (Carlton, Vic.)

    1999  Volume 4, Issue 4, Page(s) 423–426

    Abstract: Objective: An increase in incidence of reversible airflow obstruction and bronchial hyperresponsiveness occurs in patients with bronchiectasis. We conducted a study to assess the efficacy of bronchodilators in the treatment of bronchiectasis.: ... ...

    Abstract Objective: An increase in incidence of reversible airflow obstruction and bronchial hyperresponsiveness occurs in patients with bronchiectasis. We conducted a study to assess the efficacy of bronchodilators in the treatment of bronchiectasis.
    Methodology: Twenty-four patients with confirmed bronchiectasis were studied. Each patient inhaled fenoterol 400 microg administered by metered dose inhaler via a spacer after a baseline lung function and a lung function test was repeated 30 min later. This was followed by a second dose of fenoterol 5 mg via nebulizer and another lung function test 30 min later. A repeat study was done at least 24 h later with ipratropium bromide 40 microg by metered dose inhaler and 500 microg by a nebulizer.
    Results: The results showed a significant improvement from baselines (mean percentage change +/- SD) of peak expiratory flow rate (PEF) by 8.5 +/- 8.72% and 15.3 +/- 11.63%, forced expiratory volume in 1 s (FEV1) by 8.77 +/- 9.69% and 10.2 +/- 12.2% and forced vital capacity (FVC) by 10.25 +/- 11.61% and 10.09 +/- 10.88% after low- and high-dose fenoterol, respectively. The improvements after low- and high-dose ipratropium bromide for PEE FEV1 and FVC were 9.89 +/- 9.35% and 14.39 +/- 12.82%, 9.38 +/- 10.41% and 13.52 +/- 17.09%, and 8.03 +/- 10.85% and 9.63 +/- 13.85%, respectively. Eleven patients (45.8%) responded to one or both bronchodilators significantly (> 15% improvement in FEV1). Five patients (20%) responded to both, three (12%) to fenoterol alone and another three (12%) to ipratropium bromide alone.
    Conclusion: There is significant bronchodilator response in a subset of patients with bronchiectasis and patients with bronchiectasis should therefore undergo bronchodilator testing. Skin prick testing against a panel of nine allergens done on each individual yielded a positive result in 13 patients (54.2%).
    MeSH term(s) Administration, Inhalation ; Adrenergic beta-Agonists/administration & dosage ; Adult ; Aged ; Bronchiectasis/diagnosis ; Bronchiectasis/drug therapy ; Bronchodilator Agents ; Cholinergic Antagonists/administration & dosage ; Dose-Response Relationship, Drug ; Female ; Fenoterol/administration & dosage ; Humans ; Ipratropium ; Malaysia ; Male ; Middle Aged ; Prognosis ; Respiratory Function Tests ; Treatment Outcome
    Chemical Substances Adrenergic beta-Agonists ; Bronchodilator Agents ; Cholinergic Antagonists ; Fenoterol (22M9P70OQ9) ; Ipratropium (GR88G0I6UL)
    Language English
    Publishing date 1999-12
    Publishing country Australia
    Document type Clinical Trial ; Comparative Study ; Journal Article ; Lecture
    ZDB-ID 1435849-9
    ISSN 1440-1843 ; 1323-7799
    ISSN (online) 1440-1843
    ISSN 1323-7799
    DOI 10.1046/j.1440-1843.1999.00215.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Gestational diabetes mellitus--screening in a developing country.

    Samad, N / Hassan, J A / Shera, A S / Maqsood, A

    JPMA. The Journal of the Pakistan Medical Association

    1996  Volume 46, Issue 11, Page(s) 249–252

    Abstract: All gravid women attending the antenatal clinic of the department of Gynaecology Unit-II, Civil Hospital Karachi and unbooked cases presenting in labour from October, 1986 to December, 1993, were included in the study. On the basis of risk factors for ... ...

    Abstract All gravid women attending the antenatal clinic of the department of Gynaecology Unit-II, Civil Hospital Karachi and unbooked cases presenting in labour from October, 1986 to December, 1993, were included in the study. On the basis of risk factors for diabetes mellitus (DM), they were divided into 3 groups. Women in Group-I had family history of DM, bad obstetric history, congenitally malformed babies, polyhydramnios, obesity and macrosomia. Group-II included women with pregnancy induced hypertension (PIH) abruptio placentae, age > 30 years and multiple pregnancy. Group-III comprised of the pregnant women with no risk factors for DM. Screening of 4497 subjects from the antenatal clinic was performed and DM was diagnosed in 211 (12%) in Group-I, 11 (3%) in Group-II and 13 (0.6%) in Group III. Chi-square analysis demonstrated that frequency of DM was significantly higher (P < 0.001) in Group I and Group II as compared to Group III. Non-booked admissions numbered 7600, of these, DM was detected in 166 (20%) belonging to Groups-I and II, whereas, 38 (0.6%) were from Group-III. Screening was done by the O'Sullivan criteria. It was concluded that all pregnant women should be screened for DM. However, if financial constraints prevail, then women with high risk factors only should be subjected to screening.
    MeSH term(s) Adult ; Chi-Square Distribution ; Developing Countries ; Diabetes, Gestational/diagnosis ; Female ; Humans ; Mass Screening ; Pregnancy ; Risk Factors
    Language English
    Publishing date 1996-11
    Publishing country Pakistan
    Document type Journal Article
    ZDB-ID 603873-6
    ISSN 0030-9982
    ISSN 0030-9982
    Database MEDical Literature Analysis and Retrieval System OnLINE

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