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  1. Article ; Online: Neutrophil antigen antibodies affect engraftment and secondary graft failure in hematopoietic progenitor cell transplantation.

    Alswied, Abdullah / Hassan, Sajjad / Rai, Herleen / Flegel, Willy Albert

    Transfusion

    2024  

    Abstract: Background: Research is limited on the role of antibodies against human neutrophil antigen (HNA) in hematopoietic progenitor cell (HPC) transplantation outcomes.: Study design and methods: A retrospective review was conducted on medical records of ... ...

    Abstract Background: Research is limited on the role of antibodies against human neutrophil antigen (HNA) in hematopoietic progenitor cell (HPC) transplantation outcomes.
    Study design and methods: A retrospective review was conducted on medical records of patients at the NIH Clinical Center enrolled in six research protocols. This case-control study included 21 patients tested for HNA antibodies from January 2010 to March 2022 who underwent HPC transplantation. In addition, 42 patients following the same research protocols were randomly selected as a control group.
    Results: The cumulative incidence of time to neutrophil engraftment was significantly impacted by the patients' anti-HNA status (p = .042), with the patients with anti-HNA experiencing delayed engraftment. Secondary graft failure occurred in 4 out of 42 patients (9.52%; 95% confidence interval [CI]: 3.7-22.1) of the control group, while 5 out of 9 patients (55.5%; 95% CI: 26.7-81.1) with anti-HNA experienced secondary graft failure (p = .005). Furthermore, patients with anti-HNA had a lower proportion (p = .008 for full and p = .002 for partial chimerism) and cumulative incidence (p = .016 for full and p = .010 for partial chimerism) of achieving donor chimerism compared to the control group.
    Discussion: The study reveals a potential link between anti-HNA and HPC transplantation outcomes not previously reported. Patients with anti-HNA had a lower proportion and cumulative incidence of achieving donor chimerism. Additionally, anti-HNA status affected the time for neutrophil engraftment, with a slower rate of neutrophil engraftment and increased risk of secondary failure in patients with anti-HNA.
    Language English
    Publishing date 2024-04-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208417-x
    ISSN 1537-2995 ; 0041-1132
    ISSN (online) 1537-2995
    ISSN 0041-1132
    DOI 10.1111/trf.17830
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Performance of two modified two-tier algorithms for the serologic diagnosis of Lyme disease.

    Landry, Marie L / Hassan, Sajjad / Rottmann, Bruce G / Pesak, Stacey J / Ordazzo, Michael / Skrzyniarz, Michael / Deponte, Steven / Peaper, David R

    Journal of clinical microbiology

    2024  Volume 62, Issue 5, Page(s) e0013924

    Abstract: We compared the performance of a new modified two-tier testing (MTTT) platform, the Diasorin Liaison chemiluminescent immunoassay (CLIA), to the Zeus enzyme-linked immunoassay (ELISA) MTTT and to Zeus ELISA/Viramed immunoblot standard two-tier testing ( ... ...

    Abstract We compared the performance of a new modified two-tier testing (MTTT) platform, the Diasorin Liaison chemiluminescent immunoassay (CLIA), to the Zeus enzyme-linked immunoassay (ELISA) MTTT and to Zeus ELISA/Viramed immunoblot standard two-tier testing (STTT) algorithm. Of 537 samples included in this study, 91 (16.9%) were positive or equivocal by one or more screening tests. Among these 91 samples, only 57 samples were concordant positive by first-tier screening tests, and only 19 of 57 were concordant by the three second-tier methods. For IgM results, positive percent agreement (PPA) was 68.1% for Diasorin versus 89.4% for Zeus compared to immunoblot. By contrast, the PPA for IgG for both Diasorin and Zeus was 100%. Using a 2-out-of-3 consensus reference standard, the PPAs for IgM were 75.6%, 97.8%, and 95.6% for Diasorin, Zeus, and immunoblot, respectively. The difference between Zeus MTTT and Diasorin MTTT for IgM detection was significant (
    Importance: The laboratory diagnosis of Lyme disease relies upon the detection of antibodies to Borrelia species. Standard two tier testing (STTT) methods rely upon immunoblots which have clinical and technical limitations. Modified two-tier testing (MTTT) methods have recently become available and are being widely adopted. There are limited independent data available assessing the performance of MTTT and STTT methods.
    MeSH term(s) Humans ; Lyme Disease/diagnosis ; Lyme Disease/immunology ; Lyme Disease/blood ; Algorithms ; Immunoglobulin M/blood ; Immunoglobulin G/blood ; Serologic Tests/methods ; Serologic Tests/standards ; Antibodies, Bacterial/blood ; Sensitivity and Specificity ; Luminescent Measurements/methods ; Immunoblotting/methods
    Chemical Substances Immunoglobulin M ; Immunoglobulin G ; Antibodies, Bacterial
    Language English
    Publishing date 2024-04-10
    Publishing country United States
    Document type Journal Article ; Comparative Study ; Evaluation Study
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/jcm.00139-24
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: APPLICATION OF AUTOLOGOUS SERUM EYE DROPS AFTER PTERYGIUM SURGERY

    Hassan Sajjad Rathore / Ahsan Mukhtar / Shahzad Saeed / Umar Ijaz / Mamoona Javaid / Iqra Ghaus

    Pakistan Armed Forces Medical Journal, Vol 71, Iss 5, Pp 1563-

    2021  Volume 1566

    Abstract: Objective: To compare the corneal epithelium healing time and post-operative pain after pterygium surgery with and without he use of autologous serum drops. Study Design: Comparative cross-sectional study. Place and Duration of Study: Eye Department, ... ...

    Abstract Objective: To compare the corneal epithelium healing time and post-operative pain after pterygium surgery with and without he use of autologous serum drops. Study Design: Comparative cross-sectional study. Place and Duration of Study: Eye Department, Combined Military Hospital Quetta, from Jan to Aug 2019. Methodology: Twenty patients were included in this study and divided into two groups. All patients underwent pterygium excision with conjunctival autograft. Fourteen of them were instilled with autologous serum drops while 14 were given lubricants postoperatively until corneal epithelium healed completely. Corneal epithelium healing time, visual analog scale for postoperative pain assessment, conjunctival inflammation and recurrences were evaluated. Patients were followed up for 6 months. Results: For corneal epithelial healing, 1 (7.14%) patient showed complete healing on day 2 while 7 (50%) showed healing on day 3 and 6 (42.86%) on day 4. While in non-autologous serum drops group 7 (50%) showed complete healing on day 4 while 4 (28.57%) showed healing on day 5 and 3 (21.43%) on day 6. The mean corneal healing time of autologous serum drops group was 3.36 ± 0.63 days and that of non-autologous serum drops group was 4.71 ± 0.83 days. Patients of autologous serum drops group also experienced significantly less pain as compared to the non-autologous serum drops group. While the recurrence rate and conjunctival inflammation in the two groups was found to be of equal degree. Conclusion: Patients who used autologous serum eye drops post operatively experienced less pain and their corneal epithelium healed earlier than the.
    Keywords autologous serum ; corneal epithelium ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 616
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: IDENTIFICATION OF TORCH PATHOGENS IN CONGENITAL CATARACT PATIENTS VISITING ARMED FORCES INSTITUTE OF OPHTHALMOLOGY

    Mamoona Javaid / Hannan Masud / Hassan Sajjad Rathore / Dilshad Ali / Haroon Javaid

    Pakistan Armed Forces Medical Journal, Vol 71, Iss 5, Pp 1515-

    2021  Volume 1518

    Abstract: Objective: To assess the rate of positivity of Toxoplasma gondii, Rubella, Cytomegalovirus, Herpes simplex (TORCH) pathogens in patients suffering from congenital cataract in Pakistan. Study Design: Cross sectional comparative study. Place and Duration ... ...

    Abstract Objective: To assess the rate of positivity of Toxoplasma gondii, Rubella, Cytomegalovirus, Herpes simplex (TORCH) pathogens in patients suffering from congenital cataract in Pakistan. Study Design: Cross sectional comparative study. Place and Duration of Study: Armed Forces Institute of Ophthalmology, Rawalpindi, from Jun 2017 to Mar 2018. Methodology: Cataract group included sixty children already diagnosed with congenital cataract and control group included 60 children not suffering from congenital cataract. Serum samples of all children were collected to test against IgG and IgM antibodies of Toxoplasma gondii, Rubella, Cytomegalo, Herpes simplex pathogens using enzyme linked immune essay (ELISA) technique. Data were analyzed observe in difference between unilateral and bilateral cataract and between cataract and control group. Results: Out of 60 children in cataract group, 29 were tested positive for Toxoplasma gondii, Rubella, Cytomegalo (CMV), Herpes simplex infections. CMV IgG antibodies were identified in 21 (35%) children followed by Rubella IgG 13 (21.7%), Toxoplasmosis IgG 8 (13.3%), CMV IgM 7 (11.7%), HSV I IgG 5 (8.3%), HSV II IgG 2 (3.3%) and HSV I IgM 1 (1.7%) antibodies. None of the children was positive for Toxoplasmosis, Rubella and HSV II IgM antibodies. There was no statistical difference in rate of positivity of Toxoplasma gondii, Rubella, Cytomegalovirus, Herpes simplex pathogens between unilateral and bilateral cataract and between cataract and control group. Conclusion: Toxoplasma gondii, Rubella, Cytomegalovirus, Herpes simplex pathogens are an important cause of congenital cataract with Cytomegalovirus and Rubella virus being most common respectively. In order to prevent paediatric complications, we need.
    Keywords congenital cataract ; congenital infections ; cytomegalovirus ; herpes simplex virus ; rubella virus ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 616
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: SPECTRUM OF OPTIC NEUROPATHIES PRESENTING IN NEUROOPHTHALMOLOGY CLINIC AT AFIO

    Umer Ijaz / Asad Habib / Hassan Sajjad Rathore / Muhammad Imran Sarwar Khan

    Pakistan Armed Forces Medical Journal, Vol 71, Iss 1, Pp 112-

    2021  Volume 16

    Abstract: Objective: To study the spectrum of optic neuropathies in patient’s presenting at AFIO Rawalpindi. Study Design: Descriptive study/retrospective study. Place and Duration of Study: Armed Forces Institute of Ophthalmology, Rawalpindi, from Jan 2017 to Dec ...

    Abstract Objective: To study the spectrum of optic neuropathies in patient’s presenting at AFIO Rawalpindi. Study Design: Descriptive study/retrospective study. Place and Duration of Study: Armed Forces Institute of Ophthalmology, Rawalpindi, from Jan 2017 to Dec 2017. Methodology: Retrospective analysis of hospital record of patients coming to neuro-ophthalmic clinic of Armed Forces Institute of Ophthalmology (AFIO), Rawalpindi, from Jan to Dec 2017 was done. Only patients with clear final diagnosis of neurophthalmic disease were included. Those with vague diagnosis were excluded. Data was entered in SPSS version 22 and grouped into variables before analysis. Chi-square test was used for statistical analysis. The p<0.05 was considered significant. Results: Forty-five patients were included in study including 33 (73.3%) males and 12 (26.6%) females. Mean age of patients was 41.64 ± 17.74 years. Patients were grouped into ischemic optic neuropathy 20%, toxic optic neuropathies 20%, compressive optic neuropathies 17.7%, inflammatory optic neuropathies 11%, neuroretinitis 6.7%, hereditary optic neuropathies 6.7%, multiple sclerosis (MS) related 4.4%, neuromylitis optics spectrum (NMO-SD) related 4.4%, traumatic optic neuropathies 4.4% and nutritional optic neuropathies 4.4%. Deficiency of vitamin B12 and vitamin D was strongly associated with nutritional optic neuropathies while raised TLC was most common association with inflammatory optic neuropathies (57.14%) and of neuroretinitis (66%). Anti-tuberculosis drugs were most common cause of toxic optic neuropathies (88.88%). Chi square test was used for statistical analysis and p<0.05 was considered significant. Conclusion: Ischemic optic neuropathy is the most common presentation in our population according to our study. However.
    Keywords ischemic optic neuropathy ; neuropathy ; optic neuritis ; pituitary tumor ; traumatic optic neuropathy ; Medicine ; R ; Medicine (General) ; R5-920
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: COMPARATIVE EVALUATION OF TACROLIMUS 0.03% OINTMENT VS OLOPATADINE 0.2% EYE DROPS IN THE TREATMENT OF VERNAL KERATOCONJUNCTIVITIS

    Hassan Sajjad Rathore / Shahzad Saeed / M. Ahsan Mukhtar / Umar Ijaz / Asad Habib / Iqra Ghaus

    Pakistan Armed Forces Medical Journal, Vol 71, Iss 1, Pp 34-

    2021  Volume 39

    Abstract: Objective: To compare the efficacy of tacrolimus 0.03% ointment to olopatadine 0.2% eye drops in the treatment of vernal keratoconjunctivitis. Study Design: Prospective cross-sectional study. Place and Duration of Study: Eye Department Combined Military ... ...

    Abstract Objective: To compare the efficacy of tacrolimus 0.03% ointment to olopatadine 0.2% eye drops in the treatment of vernal keratoconjunctivitis. Study Design: Prospective cross-sectional study. Place and Duration of Study: Eye Department Combined Military Hospital Quetta, from Feb to Jul 2019. Methodology: A total of 69 patients with active vernal keratoconjunctivitis (VKC) were included in this study. Thirty six (52.17%) patients were randomized in tacrolimus group and 33 (47.83%) in olopatadine group B. Baseline values of the subjective symptom score (SSS) and the objective sign score (OSS) were noted. Patients were reviewed on weeks 2, 4, 8 and 12 and the scores at each visit were summed. These scores were used for comparison between groups. Results: At the start of the study, the mean subjective symptoms score and objective sign score of group A was 9.0 ± 2.04 and 3.93 ± 1.93 respectively, while that of group B was 8.88 ± 2.18 and 4.36 ± 1.90 respectively. At the end of 12-weeks, the mean subjective symptoms score and objective sign score of group A reduced to 0.11 ± 0.32 and 0.08 ± 0.28 respectively, while that of group B reduced to 1.70 ± 0.77 and 0.64 ± 0.55 respectively. Total improvement of scores (as a percentage of baselines) among tacrolimus group was 98.3% and olopatadine group was 83%. Conclusion: Although both 0.03% tacrolimus and 0.2% olopatadine were effective in improving the signs and symptoms of VKC, 0.03% tacrolimus was significantly superior.
    Keywords vernal keratoconjunctivitis ; tacrolimus ; olopatadine ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 616
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Regulatory challenges of convalescent plasma collection during the evolving stages of COVID-19 pandemic in the United States.

    Hassan, Sajjad / West, Kamille A / Conry-Cantilena, Kathleen / De Giorgi, Valeria

    Transfusion

    2021  Volume 62, Issue 2, Page(s) 483–492

    MeSH term(s) Antibodies, Viral ; Blood Donors/legislation & jurisprudence ; COVID-19/therapy ; Humans ; Immunization, Passive ; Pandemics ; SARS-CoV-2 ; United States/epidemiology
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2021-12-03
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural ; Review
    ZDB-ID 208417-x
    ISSN 1537-2995 ; 0041-1132
    ISSN (online) 1537-2995
    ISSN 0041-1132
    DOI 10.1111/trf.16751
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Rebound and overshoot of donor-specific antibodies to human leukocyte antigens (HLA) during desensitization with plasma exchanges in hematopoietic progenitor cell transplantation: A case report.

    Hassan, Sajjad / West, Kamille A / Ward, William W / Kanakry, Jennifer A / Flegel, Willy A

    Transfusion

    2021  Volume 61, Issue 6, Page(s) 1980–1986

    Abstract: Background: Donor-specific antibodies (DSA) to HLA have been associated with graft loss in hematopoietic progenitor cell (HPC) transplantation. Limited data associate therapeutic plasma exchange (TPE) with desensitization and successful engraftment. We ... ...

    Abstract Background: Donor-specific antibodies (DSA) to HLA have been associated with graft loss in hematopoietic progenitor cell (HPC) transplantation. Limited data associate therapeutic plasma exchange (TPE) with desensitization and successful engraftment. We report an attempt of desensitization and observed overshooting of DSA during transplantation.
    Case report and results: A 27-year-old female with cutaneous T cell lymphoma was scheduled for HPC transplantation from her HLA-haploidentical half-sister, who carried the HLA-DRB1*13:03:01 allele. The patient had the corresponding DSA. Lacking an alternative donor option at the time, we attempted a desensitization approach by immunosuppression with tacrolimus and mycophenolate mofetil (MMF). Unexpectedly, DSA increased from a mean fluorescence intensity (MFI) of 1835 on day -63 to 9008 on day -7. The MFI increased further during 3 TPE procedures and intravenous immunoglobulin (IVIG) until day -1. After transplantation, the DSA remained elevated despite 2 more TPE/IVIG procedures and graft-versus-host disease prophylaxis with high-dose cyclophosphamide, sirolimus, and MMF. Flow cytometric crossmatch, initially negative, turned positive after transplantation. Primary graft failure occurred and was attributed to antibody-mediated rejection. A second transplantation from a 7/8 HLA-matched unrelated donor, not carrying DRB1*13:03 allele, resulted in successful engraftment.
    Conclusion: Unexpected and rapid increases of a DSA can occur despite the use of current desensitization approaches. This is problematic when conditioning has already started, as such increases are unlikely to be overcome by TPE or other interventions for desensitization. Overshoot of DSA in HPC transplantation has rarely been reported. Its cause remains unclear and can include underlying disease, immunotherapy, chemotherapy, or TPE.
    MeSH term(s) Adult ; Antibodies/blood ; Antibodies/immunology ; Female ; HLA Antigens/blood ; HLA Antigens/immunology ; Hematopoietic Stem Cell Transplantation ; Humans ; Immunosuppression Therapy ; Lymphoma, T-Cell, Cutaneous/blood ; Lymphoma, T-Cell, Cutaneous/immunology ; Lymphoma, T-Cell, Cutaneous/therapy ; Plasma Exchange ; Tissue Donors
    Chemical Substances Antibodies ; HLA Antigens
    Language English
    Publishing date 2021-04-26
    Publishing country United States
    Document type Case Reports ; Research Support, N.I.H., Extramural
    ZDB-ID 208417-x
    ISSN 1537-2995 ; 0041-1132
    ISSN (online) 1537-2995
    ISSN 0041-1132
    DOI 10.1111/trf.16411
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: A Rare Presentation of Cyclitis Induced Myopia.

    Ijaz, Umar / Habib, Asad / Rathore, Hassan Sajjad

    Journal of the College of Physicians and Surgeons--Pakistan : JCPSP

    2018  Volume 28, Issue 3, Page(s) S56–S57

    Abstract: Unilateral cyclitis leading to myopia is a rare and clinical relevant entity. In clinical settings, pseudomyopia is generally encountered in the form of accommodative spasm, which is always bilateral. Cyclitis due to inflammation, on the other hand, can ... ...

    Abstract Unilateral cyclitis leading to myopia is a rare and clinical relevant entity. In clinical settings, pseudomyopia is generally encountered in the form of accommodative spasm, which is always bilateral. Cyclitis due to inflammation, on the other hand, can cause pseudomyopia unilaterally and it is a very rare presentation. A young male with acute anterior uveitis, presented with acute episode of unilateral myopia. When patient was examined on first visit, there were no cells in anterior chamber; so he was started on cycloplegic eye drops, but his condition didn't improve. Examination on subsequent visit revealed cellular reaction in anterior chamber and narrowing of anterior chamber angles on anterior segment optical coherence tomography (OCT). Treatment for uveitis was started and patient's visual acuity and refractive error improved. Pseudomyopia is a known complication of several drugs and certain medical conditions. The possible mechanism is supraciliary exudation causing relaxation of zonular fibers and increased convexity of the crystalline lens. Myopia in the setting of a mild cellular reaction can easily be missed and has not been reported yet to the best of authors' literature search.
    MeSH term(s) Adult ; Anterior Chamber/pathology ; Humans ; Male ; Muscle Spasticity/drug therapy ; Muscle Spasticity/etiology ; Mydriatics/therapeutic use ; Myopia/etiology ; Refractive Errors ; Therapeutics ; Tomography, Optical Coherence ; Uveitis/diagnosis ; Uveitis/drug therapy ; Visual Acuity/physiology
    Chemical Substances Mydriatics
    Language English
    Publishing date 2018-02-26
    Publishing country Pakistan
    Document type Case Reports ; Journal Article
    ZDB-ID 2276646-7
    ISSN 1681-7168 ; 1022-386X
    ISSN (online) 1681-7168
    ISSN 1022-386X
    DOI 10.29271/jcpsp.2018.03.S56
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: COMPARISON OF PAIN PERCEIVED BY PATIENTS UNDERGOING INTRA ORAL LOCAL ANESTHESIA USING DIFFERENT NEEDLE GAUGES

    NoorUlSabah Hussain / Shoaib Younus / Uzair Bin Akhtar / Malik Ali Hassan Sajjad / Muhammad Salman Chishty / Saud Iqbal

    Pakistan Armed Forces Medical Journal, Vol 70, Iss 6, Pp 1702-

    2020  Volume 1706

    Abstract: Objective: To compare the difference in pain perceived by patients undergoing intra-oral local anesthesia with different gauge needles. Study Design: Qausi experimental study. Place and Duration of Study: Oral and Maxillofacial surgery department of ... ...

    Abstract Objective: To compare the difference in pain perceived by patients undergoing intra-oral local anesthesia with different gauge needles. Study Design: Qausi experimental study. Place and Duration of Study: Oral and Maxillofacial surgery department of Institute of Dentistry, Combined Military Hospital, Lahore Medical College, Lahore, from July 2019 to August 2019. Methodology: One hundred patients were selected by consecutive sampling from the oral surgery OPD clinic. They were divided into two groups A and B randomly. Twenty three gauge needle on a 3cc disposable syringe was used for inferior alveolar nerve block and buccal nerve block for group A and 27 gauge needle on a metal dental syringe was used for the same in group B. Patients gave a verbal pain score, from 0-10 for each injection. Results: One Hundred participants were included in study, 37 (37%) males and 63 (63%) females. Mean pain score for group A for the inferior alveolar nerve block was 4.50 ± 2.1 and group B was 3.86 ± 2. The mean pain score for the buccal nerve block in group A was 4.02 ± 1.7, while that of group B was 3.94 ± 1.8. There was no significant difference (p=0.167 & 0.855) in pain perceived by patients undergoing intra oral local anesthesia using needles of different gauges. Conclusion: There is no difference in pain perceived by patients undergoing intra oral local anesthesia using needles of different gauges.
    Keywords oral sugery ; local anesthesia ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 616
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher Army Medical College Rawalpindi
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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