Article ; Online: Aspergillus fumigatus-Specific T Cells in Patients with Chronic Rhinosinusitis.
International archives of allergy and immunology
2023 Volume 184, Issue 5, Page(s) 502–512
Abstract: Introduction: Aspergillus fumigatus belongs to the saprophytic fungi, and its spores form a significant part of the daily load of fungal spores inhaled as particles in aerosols. A. fumigatus is a possible T-cell activator. Its contribution to the ... ...
| Abstract | Introduction: Aspergillus fumigatus belongs to the saprophytic fungi, and its spores form a significant part of the daily load of fungal spores inhaled as particles in aerosols. A. fumigatus is a possible T-cell activator. Its contribution to the pathogenesis of chronic rhinosinusitis (CRS) is controversially discussed. The aim of this study was to detect and characterize A. fumigatus-specific CD4+ and CD8+ T cells in patients with CRS with (CRSwNP) and without (CRSsNP) nasal polyps. Methods: Tissue and blood samples were collected from patients who underwent paranasal sinus surgery due to CRSwNP or CRSsNP. Afterward, purified CD4+ and CD8+ cells were cultured together with antigen-presenting cells. A peptide mix derived from A. fumigatus antigen was added to the cultures. After 6 days, multicolor flow cytometry was performed, and proliferation was measured using the marker Ki-67. Cytokine secretion was quantified from the supernatant of the cell culture. Results: Significant differences in the proliferation of nasal CD4+ T cells to A. fumigatus antigen were observed for cells from patients with CRSwNP in comparison to CRSsNP, while no differences were found between nasal and peripheral blood T cells. The activation of tissue-derived CD4+ T cells was associated with significantly higher concentrations of IL-4, IL-5, and IL-17a in the cell culture from patients with CRSwNP in comparison to CRSsNP and/or healthy controls. Conclusion: Our findings indicate that patients with CRSwNP harbor a higher proportion of A. fumigatus-reactive CD4+ T cells in the nasal mucosa than patients with CRSsNP. A. fumigatus-reactive CD4+ T cells of CRSwNP patients secreted TH2 cytokines and IL-17. Our findings suggest a role for A. fumigatus in the pathogenesis of CRSwNP and provide a rationale for targeted therapies. |
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| MeSH term(s) | Humans ; Aspergillus fumigatus ; Rhinitis ; Sinusitis ; Nasal Mucosa ; CD4-Positive T-Lymphocytes ; Chronic Disease ; Nasal Polyps |
| Language | English |
| Publishing date | 2023-01-18 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 1108932-5 |
| ISSN | 1423-0097 ; 1018-2438 |
| ISSN (online) | 1423-0097 |
| ISSN | 1018-2438 |
| DOI | 10.1159/000528394 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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