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Book ; Online ; Thesis: Humorale Immunantwort nach monovalenter, adjuvantierter Impfung gegen die pandemische Influenza H1N1 (2009)

Hauka, Sandra [Verfasser] / Adams, Ortwin [Gutachter] / MacKenzie, Colin [Gutachter]

2023

Author's details	Sandra Hauka ; Gutachter: Ortwin Adams, Colin MacKenzie
Keywords	Medizin, Gesundheit ; Medicine, Health
Subject code	sg610
Language	German
Publisher	Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf
Publishing place	Düsseldorf
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

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Article ; Online: Real-world performance of the NeuMoDx™ HCV Quant Test for quantification of hepatitis C virus (HCV)-RNA.

Lübke, Nadine / Walker, Andreas / Obermeier, Martin / Camdereli, Jennifer / Paluschinski, Martha / Walotka, Lara / Schupp, Anna-Kathrin / Tometten, Inga / Hauka, Sandra / Heger, Eva / Timm, Jörg

Journal of virological methods

2024 Volume 327, Page(s) 114937

Abstract: Quantification of hepatitis C virus (HCV)-RNA in serum or plasma samples is an essential parameter in HCV diagnostics. Here, the NeuMoDx™Molecular System (Qiagen) was tested for the most common HCV genotypes and compared to the cobas c6800 system (Roche). ...

Abstract	Quantification of hepatitis C virus (HCV)-RNA in serum or plasma samples is an essential parameter in HCV diagnostics. Here, the NeuMoDx™Molecular System (Qiagen) was tested for the most common HCV genotypes and compared to the cobas c6800 system (Roche). HCV-RNA from 131 plasma/serum samples from chronically infected patients was determined in parallel on the NeuMoDx and c6800 systems. Linearity was analysed using the four most common HCV genotypes (1-4) in our cohort. The coefficient of variation (CV) within (intra-assay) and between (inter-assay) runs was calculated based on HCV-RNA concentration. Quantitative HCV-RNA results were highly correlated on both test systems (R
Language	English
Publishing date	2024-04-11
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2024.114937
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Article ; Online: Adjusted COVID-19 booster schedules balance age-dependent differences in antibody titers benefitting risk populations.

Müller, Lisa / Andrée, Marcel / Moskorz, Wiebke / Drexler, Ingo / Hauka, Sandra / Ptok, Johannes / Walotka, Lara / Grothmann, Ramona / Hillebrandt, Jonas / Ritchie, Anastasia / Peter, Laura / Walker, Andreas / Timm, Jörg / Adams, Ortwin / Schaal, Heiner

Frontiers in aging

2022 Volume 3, Page(s) 1027885

Abstract: We provide follow-up data on the humoral immune response after COVID-19 vaccinations of two distinct cohorts aged below 60 and over 80 years to screen for age-related differences in the longevity and magnitude of the induction of the antibody responses ... ...

Abstract	We provide follow-up data on the humoral immune response after COVID-19 vaccinations of two distinct cohorts aged below 60 and over 80 years to screen for age-related differences in the longevity and magnitude of the induction of the antibody responses post booster-vaccinations. While anti-SARS-CoV-2 spike-specific IgG and neutralization capacity waned rapidly after the initial vaccination schedule, additional boosters highly benefitted the humoral immune responses especially in the elderly cohort, including the neutralization of Omikron variants. Thus, adjusted COVID-19 booster vaccination schedules are an appropriate tool to overcome limitations in the success of vaccinations.
Language	English
Publishing date	2022-10-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	3076785-4
ISSN	2673-6217 ; 2673-6217
ISSN (online)	2673-6217
ISSN	2673-6217
DOI	10.3389/fragi.2022.1027885
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Article ; Online: Extraction-free SARS-CoV-2 detection by rapid RT-qPCR universal for all primary respiratory materials.

Lübke, Nadine / Senff, Tina / Scherger, Sara / Hauka, Sandra / Andrée, Marcel / Adams, Ortwin / Timm, Jörg / Walker, Andreas

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

2020 Volume 130, Page(s) 104579

Abstract: Background: Fast and reliable detection of SARS-CoV-2 is crucial for efficient control of the COVID-19 pandemic. Due to the high demand for SARS-CoV-2 testing there is a worldwide shortage of RNA extraction reagents. Therefore, extraction-free RT-qPCR ... ...

Abstract	Background: Fast and reliable detection of SARS-CoV-2 is crucial for efficient control of the COVID-19 pandemic. Due to the high demand for SARS-CoV-2 testing there is a worldwide shortage of RNA extraction reagents. Therefore, extraction-free RT-qPCR protocols are urgently needed. Objectives: To establish a rapid RT-qPCR protocol for the detection of SARS-CoV-2 without the need of RNA extraction suitable for all respiratory materials. Material and methods: Different SARS-CoV-2 positive respiratory materials from our routine laboratory were used as crude material after heat inactivation in direct RT-qPCR with the PrimeDirect™ Probe RT-qPCR Mix (TaKaRa). SARS-CoV-2 was detected using novel primers targeted to the E-gene. Results: The protocol for the detection of SARS-CoV-2 in crude material used a prepared frozen-PCR mix with optimized primers and 5 μl of fresh, undiluted and pre-analytically heat inactivated respiratory material. For validation, 91 respiratory samples were analyzed in direct comparison to classical RNA-based RT-qPCR. Overall 81.3 % of the samples were detected in both assays with a strong correlation between both Ct values (r = 0.8492, p < 0.0001). The SARS-CoV-2 detection rate by direct RT-qPCR was 95.8 % for Ct values <35. All negative samples were characterized by low viral loads (Ct >35) and/or long storage times before sample processing. Conclusion: Direct RT-qPCR is a suitable alternative to classical RNA RT-qPCR, provided that only fresh samples (storage <1 week) are used. RNA extraction should be considered if samples have longer storage times or if PCR inhibition is observed. In summary, this protocol is fast, inexpensive and suitable for all respiratory materials.
MeSH term(s)	Betacoronavirus ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Coronavirus Infections/diagnosis ; Coronavirus Infections/virology ; DNA Primers/genetics ; Humans ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/virology ; RNA, Viral/analysis ; Real-Time Polymerase Chain Reaction/methods ; Respiratory System/virology ; SARS-CoV-2 ; Sensitivity and Specificity ; Specimen Handling/methods ; Time Factors
Chemical Substances	DNA Primers ; RNA, Viral
Keywords	covid19
Language	English
Publishing date	2020-08-05
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1446080-4
ISSN	1873-5967 ; 1386-6532
ISSN (online)	1873-5967
ISSN	1386-6532
DOI	10.1016/j.jcv.2020.104579
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Article ; Online: Adjusted booster schedules disperse age-dependent differences in antibody titers benefitting risk populations - Update to: Age-dependent Immune Response to the BioNTech/Pfizer BNT162b2 Coronavirus Disease 2019 Vaccination

medRxiv

Abstract: We provide follow-up data on the humoral immune response after COVID-19 vaccinations of a cohort aged below 60 and over 80 years. While anti-SARS-CoV-2 spike-specific IgG and neutralization capacity waned rapidly after initial vaccination, additional ... ...

Abstract	We provide follow-up data on the humoral immune response after COVID-19 vaccinations of a cohort aged below 60 and over 80 years. While anti-SARS-CoV-2 spike-specific IgG and neutralization capacity waned rapidly after initial vaccination, additional boosters highly benefitted humoral immune responses including neutralization of Omikron variants in the elderly cohort.
Keywords	covid19
Language	English
Publishing date	2022-08-09
Publisher	Cold Spring Harbor Laboratory Press
Document type	Article ; Online
DOI	10.1101/2022.08.08.22278545
Database	COVID19

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Article ; Online: Extraction-free SARS-CoV-2 detection by rapid RT-qPCR universal for all primary respiratory materials

Lübke, Nadine / Senff, Tina / Scherger, Sara / Hauka, Sandra / Andrée, Marcel / Adams, Ortwin / Timm, Jörg / Walker, Andreas

Journal of Clinical Virology

2020 Volume 130, Page(s) 104579

Keywords	Virology ; Infectious Diseases ; covid19
Language	English
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
ZDB-ID	1446080-4
ISSN	1873-5967 ; 1386-6532
ISSN (online)	1873-5967
ISSN	1386-6532
DOI	10.1016/j.jcv.2020.104579
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Extraction-free SARS-CoV-2 detection by rapid RT-qPCR universal for all primary respiratory materials

Lübke, Nadine / Senff, Tina / Scherger, Sara / Hauka, Sandra / Andrée, Marcel / Adams, Ortwin / Timm, Jörg / Walker, Andreas

J Clin Virol

Abstract: BACKGROUND: Fast and reliable detection of SARS-CoV-2 is crucial for efficient control of the COVID-19 pandemic. Due to the high demand for SARS-CoV-2 testing there is a worldwide shortage of RNA extraction reagents. Therefore, extraction-free RT-qPCR ... ...

Abstract	BACKGROUND: Fast and reliable detection of SARS-CoV-2 is crucial for efficient control of the COVID-19 pandemic. Due to the high demand for SARS-CoV-2 testing there is a worldwide shortage of RNA extraction reagents. Therefore, extraction-free RT-qPCR protocols are urgently needed. OBJECTIVES: To establish a rapid RT-qPCR protocol for the detection of SARS-CoV-2 without the need of RNA extraction suitable for all respiratory materials. MATERIAL AND METHODS: Different SARS-CoV-2 positive respiratory materials from our routine laboratory were used as crude material after heat inactivation in direct RT-qPCR with the PrimeDirect™ Probe RT-qPCR Mix (TaKaRa). SARS-CoV-2 was detected using novel primers targeted to the E-gene. RESULTS: The protocol for the detection of SARS-CoV-2 in crude material used a prepared frozen-PCR mix with optimized primers and 5 µl of fresh, undiluted and pre-analytically heat inactivated respiratory material. For validation, 91 respiratory samples were analyzed in direct comparison to classical RNA-based RT-qPCR. Overall 81.3 % of the samples were detected in both assays with a strong correlation between both Ct values (r = 0.8492, p < 0.0001). The SARS-CoV-2 detection rate by direct RT-qPCR was 95.8 % for Ct values <35. All negative samples were characterized by low viral loads (Ct >35) and/or long storage times before sample processing. CONCLUSION: Direct RT-qPCR is a suitable alternative to classical RNA RT-qPCR, provided that only fresh samples (storage <1 week) are used. RNA extraction should be considered if samples have longer storage times or if PCR inhibition is observed. In summary, this protocol is fast, inexpensive and suitable for all respiratory materials.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #694331
Database	COVID19

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Article: Secondary sclerosing cholangitis as a complication of severe COVID-19: A case report and review of the literature.

Klindt, Caroline / Jensen, Björn-Erik / Brandenburger, Timo / Feldt, Torsten / Killer, Alexander / Schimmöller, Lars / Antoch, Gerald / Senff, Tina / Hauka, Sandra / Timm, Jörg / Bahners, Bahne Hendrik / Seidl, Maximilian / Esposito, Irene / Luedde, Tom / Bode, Johannes G / Keitel, Verena

Clinical case reports

2021 Volume 9, Issue 5, Page(s) e04068

Abstract: This case of secondary sclerosing cholangitis (SSC-CIP) emphasizes the need to provide follow-up care for patients that have recovered from COVID-19 in order to understand the complexity of SARS-CoV-2 associated sequela. ...

Abstract	This case of secondary sclerosing cholangitis (SSC-CIP) emphasizes the need to provide follow-up care for patients that have recovered from COVID-19 in order to understand the complexity of SARS-CoV-2 associated sequela.
Language	English
Publishing date	2021-05-24
Publishing country	England
Document type	Case Reports
ZDB-ID	2740234-4
ISSN	2050-0904
ISSN	2050-0904
DOI	10.1002/ccr3.4068
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Article ; Online: Age-dependent Immune Response to the Biontech/Pfizer BNT162b2 Coronavirus Disease 2019 Vaccination.

Müller, Lisa / Andrée, Marcel / Moskorz, Wiebke / Drexler, Ingo / Walotka, Lara / Grothmann, Ramona / Ptok, Johannes / Hillebrandt, Jonas / Ritchie, Anastasia / Rabl, Denise / Ostermann, Philipp Niklas / Robitzsch, Rebekka / Hauka, Sandra / Walker, Andreas / Menne, Christopher / Grutza, Ralf / Timm, Jörg / Adams, Ortwin / Schaal, Heiner

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

2021 Volume 73, Issue 11, Page(s) 2065–2072

Abstract: Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has led to the development of various vaccines. Real-life data on immune responses elicited in the most vulnerable group of vaccinees older than age 80 years old are ... ...

Abstract	Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has led to the development of various vaccines. Real-life data on immune responses elicited in the most vulnerable group of vaccinees older than age 80 years old are still underrepresented despite the prioritization of the elderly in vaccination campaigns. Methods: We conducted a cohort study with 2 age groups, young vaccinees below the age of 60 years and elderly vaccinees over the age of 80 years, to compare their antibody responses to the first and second dose of the BNT162b2 coronavirus disease 2019 vaccination. Results: Although the majority of participants in both groups produced specific immunoglobulin G antibody titers against SARS-CoV-2 spike protein, titers were significantly lower in elderly participants. Although the increment of antibody levels after the second immunization was higher in elderly participants, the absolute mean titer of this group remained lower than the <60 years of age group. After the second vaccination, 31.3% of the elderly had no detectable neutralizing antibodies in contrast to the younger group, in which only 2.2% had no detectable neutralizing antibodies. Conclusions: Our data showed differences between the antibody responses raised after the first and second BNT162b2 vaccination, in particular lower frequencies of neutralizing antibodies in the elderly group. This suggests that this population needs to be closely monitored and may require earlier revaccination and/or an increased vaccine dose to ensure stronger long-lasting immunity and protection against infection.
MeSH term(s)	Age Factors ; Aged ; Aged, 80 and over ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; BNT162 Vaccine/immunology ; COVID-19/prevention & control ; Cohort Studies ; Female ; Humans ; Immunity ; Immunoglobulin G/blood ; Male ; Middle Aged ; Spike Glycoprotein, Coronavirus/immunology ; Vaccination
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; BNT162 Vaccine (N38TVC63NU)
Language	English
Publishing date	2021-04-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1099781-7
ISSN	1537-6591 ; 1058-4838
ISSN (online)	1537-6591
ISSN	1058-4838
DOI	10.1093/cid/ciab381
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Article ; Online: Sensitivity of anti-SARS-CoV-2 serological assays in a high-prevalence setting.

Müller, Lisa / Ostermann, Philipp Niklas / Walker, Andreas / Wienemann, Tobias / Mertens, Alexander / Adams, Ortwin / Andree, Marcel / Hauka, Sandra / Lübke, Nadine / Keitel, Verena / Drexler, Ingo / Di Cristanziano, Veronica / Hermsen, Derik Franz / Kaiser, Rolf / Boege, Friedrich / Klein, Florian / Schaal, Heiner / Timm, Jörg / Senff, Tina

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

2021 Volume 40, Issue 5, Page(s) 1063–1071

Abstract: Evaluation and power of seroprevalence studies depend on the performed serological assays. The aim of this study was to assess four commercial serological tests from EUROIMMUN, DiaSorin, Abbott, and Roche as well as an in-house immunofluorescence and ... ...

Abstract	Evaluation and power of seroprevalence studies depend on the performed serological assays. The aim of this study was to assess four commercial serological tests from EUROIMMUN, DiaSorin, Abbott, and Roche as well as an in-house immunofluorescence and neutralization test for their capability to identify SARS-CoV-2 seropositive individuals in a high-prevalence setting. Therefore, 42 social and working contacts of a German super-spreader were tested. Consistent with a high-prevalence setting, 26 of 42 were SARS-CoV-2 seropositive by neutralization test (NT), and immunofluorescence test (IFT) confirmed 23 of these 26 positive test results (NT 61.9% and IFT 54.8% seroprevalence). Four commercial assays detected anti-SARS-CoV-2 antibodies in 33.3-40.5% individuals. Besides an overall discrepancy between the NT and the commercial assays regarding their sensitivity, this study revealed that commercial SARS-CoV-2 spike-based assays are better to predict the neutralization titer than nucleoprotein-based assays are.
MeSH term(s)	Adolescent ; Adult ; Aged ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; COVID-19/blood ; COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19 Serological Testing/methods ; COVID-19 Serological Testing/standards ; Contact Tracing ; Female ; Humans ; Immunoassay ; Male ; Middle Aged ; Neutralization Tests ; Prevalence ; SARS-CoV-2/immunology ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Young Adult
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral
Language	English
Publishing date	2021-02-03
Publishing country	Germany
Document type	Comparative Study ; Journal Article
ZDB-ID	603155-9
ISSN	1435-4373 ; 0934-9723 ; 0722-2211
ISSN (online)	1435-4373
ISSN	0934-9723 ; 0722-2211
DOI	10.1007/s10096-021-04169-7
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