LIVIVO - Search results -

Search results

Result 1 - 10 of total 13

Search options

Article: Metastatic Canine Phaeochromocytoma with Unusual Manifestation

Gregor, Katharina M. / Knebel, Anna / Haverkamp, Ann-Kathrin / Baumgärtner, Wolfgang / Volk, Holger

Journal of comparative pathology. 2022 Apr., v. 192

2022

Abstract: We report the clinical, pathological and immunohistochemical characteristics of a phaeochromocytoma (PCC) in a 9-year-old male neutered Golden Retriever dog. The dog presented with acute onset of deteriorating cervical pain but was otherwise normal on ... ...

Abstract	We report the clinical, pathological and immunohistochemical characteristics of a phaeochromocytoma (PCC) in a 9-year-old male neutered Golden Retriever dog. The dog presented with acute onset of deteriorating cervical pain but was otherwise normal on general physical and neurological examinations. Magnetic resonance imaging of the cervical spine revealed a focal, extramedullary, infiltrative, poorly demarcated, heterogeneous mass with moderate contrast enhancement at the left cranial articular process of C2, associated with osteolysis and pathological fractures of C2, and marked soft tissue trauma. Due to the severe lesions and grave prognosis, the dog was euthanized. Post-mortem examination revealed severe enlargement of the right adrenal gland due to a neoplasm of the adrenal medulla. C2 was lytic and there was a white, well-demarcated, firm neoplastic mass in the surrounding musculature. Neoplasms were also present in, and adjacent to, the prostate gland and in pulmonary lymph nodes. Histologically, the neoplasms were composed of dense sheets and nests of small, round to polyhedral cells with frequent palisading along fine connective tissue septa, karyomegaly, multinucleated cells and frequent mitotic figures. Immunohistochemically, neoplastic cells expressed chromogranin A and synaptophysin. Gross, microscopic and immunohistochemical findings support the diagnosis of PCC, originating from the right adrenal gland, with multiple metastases in the cervical spine, prostate gland and pulmonary lymph nodes. This case highlights the difficulty of intra-vitam diagnosis of PCC as its manifestation can be highly variable. PCC should be considered as a rare but possible differential diagnosis for painful vertebral masses in elderly dogs.
Keywords	Golden Retriever ; adrenal medulla ; bone resorption ; cervical spine ; dogs ; elderly ; immunohistochemistry ; lymph ; magnetism ; males ; metastasis ; mitosis ; necropsy ; pain ; prognosis ; prostate gland
Language	English
Dates of publication	2022-04
Size	p. 33-40.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	390920-7
ISSN	1532-3129 ; 0021-9975
ISSN (online)	1532-3129
ISSN	0021-9975
DOI	10.1016/j.jcpa.2022.01.006
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Ud II Zs.195: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: Metastatic Canine Phaeochromocytoma with Unusual Manifestation.

Gregor, Katharina M / Knebel, Anna / Haverkamp, Ann-Kathrin / Baumgärtner, Wolfgang / Volk, Holger

Journal of comparative pathology

2022 Volume 192, Page(s) 33–40

Abstract	We report the clinical, pathological and immunohistochemical characteristics of a phaeochromocytoma (PCC) in a 9-year-old male neutered Golden Retriever dog. The dog presented with acute onset of deteriorating cervical pain but was otherwise normal on general physical and neurological examinations. Magnetic resonance imaging of the cervical spine revealed a focal, extramedullary, infiltrative, poorly demarcated, heterogeneous mass with moderate contrast enhancement at the left cranial articular process of C2, associated with osteolysis and pathological fractures of C2, and marked soft tissue trauma. Due to the severe lesions and grave prognosis, the dog was euthanized. Post-mortem examination revealed severe enlargement of the right adrenal gland due to a neoplasm of the adrenal medulla. C2 was lytic and there was a white, well-demarcated, firm neoplastic mass in the surrounding musculature. Neoplasms were also present in, and adjacent to, the prostate gland and in pulmonary lymph nodes. Histologically, the neoplasms were composed of dense sheets and nests of small, round to polyhedral cells with frequent palisading along fine connective tissue septa, karyomegaly, multinucleated cells and frequent mitotic figures. Immunohistochemically, neoplastic cells expressed chromogranin A and synaptophysin. Gross, microscopic and immunohistochemical findings support the diagnosis of PCC, originating from the right adrenal gland, with multiple metastases in the cervical spine, prostate gland and pulmonary lymph nodes. This case highlights the difficulty of intra-vitam diagnosis of PCC as its manifestation can be highly variable. PCC should be considered as a rare but possible differential diagnosis for painful vertebral masses in elderly dogs.
MeSH term(s)	Adrenal Gland Neoplasms/diagnosis ; Adrenal Gland Neoplasms/veterinary ; Animals ; Diagnosis, Differential ; Dog Diseases/pathology ; Dogs ; Male ; Peripheral Nervous System Neoplasms/veterinary ; Pheochromocytoma/diagnosis ; Pheochromocytoma/veterinary
Language	English
Publishing date	2022-02-23
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	390920-7
ISSN	1532-3129 ; 0021-9975
ISSN (online)	1532-3129
ISSN	0021-9975
DOI	10.1016/j.jcpa.2022.01.006
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Ud II Zs.195: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Middle East respiratory syndrome coronavirus infection in camelids

Te, Nigeer / Ciurkiewicz, Malgorzata / van den Brand, Judith M. A. / Rodon, Jordi / Haverkamp, Ann-Kathrin / Vergara-Alert, Júlia / Bensaid, Albert / Haagmans, Bart L. / Baumgartner, Wolfgang / Segalés, Joaquim

Veterinary pathology. 2022 July, v. 59, no. 4

2022

Abstract: Middle East respiratory syndrome coronavirus (MERS-CoV) is the cause of a severe respiratory disease with a high case fatality rate in humans. Since its emergence in mid-2012, 2578 laboratory-confirmed cases in 27 countries have been reported by the ... ...

Abstract	Middle East respiratory syndrome coronavirus (MERS-CoV) is the cause of a severe respiratory disease with a high case fatality rate in humans. Since its emergence in mid-2012, 2578 laboratory-confirmed cases in 27 countries have been reported by the World Health Organization, leading to 888 known deaths due to the disease and related complications. Dromedary camels are considered the major reservoir host for this virus leading to zoonotic infection in humans. Dromedary camels, llamas, and alpacas are susceptible to MERS-CoV, developing a mild-to-moderate upper respiratory tract infection characterized by epithelial hyperplasia as well as infiltration of neutrophils, lymphocytes, and some macrophages within epithelium, lamina propria, in association with abundant viral antigen. The very mild lesions in the lower respiratory tract of these camelids correlate with absence of overt illness following MERS-CoV infection. Unfortunately, there is no approved antiviral treatment or vaccine for MERS-CoV infection in humans. Thus, there is an urgent need to develop intervention strategies in camelids, such as vaccination, to minimize virus spillover to humans. Therefore, the development of camelid models of MERS-CoV infection is key not only to assess vaccine prototypes but also to understand the biologic mechanisms by which the infection can be naturally controlled in these reservoir species. This review summarizes information on virus-induced pathological changes, pathogenesis, viral epidemiology, and control strategies in camelids, as the intermediate hosts and primary source of MERS-CoV infection in humans.
Keywords	Camelus dromedarius ; Coronavirus infections ; Middle East respiratory syndrome coronavirus ; World Health Organization ; animal pathology ; epidemiology ; epithelium ; hyperplasia ; macrophages ; mortality ; neutrophils ; pathogenesis ; respiratory system ; respiratory tract diseases ; vaccination ; vaccines ; viral antigens ; viruses ; zoonoses
Language	English
Dates of publication	2022-07
Size	p. 546-555.
Publishing place	SAGE Publications
Document type	Article
ZDB-ID	188012-3
ISSN	1544-2217 ; 0300-9858
ISSN (online)	1544-2217
ISSN	0300-9858
DOI	10.1177/03009858211069120
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 1959: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: A new genotype of hepatitis A virus causing transient liver enzyme elevations in Mauritius-origin laboratory-housed

Mecklenburg, Lars / Ducore, Rebecca / Boyle, Molly / Newell, Andrew / Boone, Laura / Luft, Joerg / Romeike, Annette / Haverkamp, Ann-Kathrin / Mansfield, Keith / Penraat, Kelley A / Baczenas, J J / Minor, Nick / O'Connor, Shelby L / O'Connor, David H

Veterinary pathology

2023 Volume 61, Issue 3, Page(s) 488–496

Abstract: Hepatitis A virus (HAV) infects humans and nonhuman primates, typically causing an acute self-limited illness. Three HAV genotypes have been described so far for humans, and three genotypes have been described for nonhuman primates. We observed ... ...

Abstract	Hepatitis A virus (HAV) infects humans and nonhuman primates, typically causing an acute self-limited illness. Three HAV genotypes have been described so far for humans, and three genotypes have been described for nonhuman primates. We observed transiently elevated liver enzymes in Mauritius-origin laboratory-housed macaques in Germany and were not able to demonstrate an etiology including HAV by serology and polymerase chain reaction (PCR). HAV is a rare pathogen in cynomolgus macaques, and since all employees were routinely vaccinated against HAV, it was not a part of the routine vaccination and screening program. A deep sequencing approach identified a new HAV genotype (referred to as Simian_HAV_Macaca/Germany/Mue-1/2022) in blood samples from affected animals. This HAV was demonstrated by reverse transcription PCR in blood and liver and by
MeSH term(s)	Animals ; Macaca fascicularis/virology ; Hepatitis A/veterinary ; Hepatitis A/virology ; Genotype ; Liver/virology ; Hepatitis A virus/genetics ; Monkey Diseases/virology ; Mauritius ; Phylogeny ; Germany ; Male ; Female ; Animals, Laboratory/virology
Language	English
Publishing date	2023-11-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	188012-3
ISSN	1544-2217 ; 0300-9858
ISSN (online)	1544-2217
ISSN	0300-9858
DOI	10.1177/03009858231209691
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 629: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein

Haverkamp, Ann-Kathrin / Bosch, Berend J. / Spitzbarth, Ingo / Lehmbecker, Annika / Te, Nigeer / Bensaid, Albert / Segalés, Joaquim / Baumgärtner, Wolfgang

Veterinary immunology and immunopathology. 2019 Dec., v. 218

2019

Abstract: Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in ...

Abstract	Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.
Keywords	Coronavirus infections ; cytoplasm ; disease prevention ; epitopes ; humans ; immune response ; immunohistochemistry ; immunopathology ; membrane fusion ; nose ; respiratory mucosa ; respiratory tract diseases
Language	English
Dates of publication	2019-12
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	754160-0
ISSN	1873-2534 ; 0165-2427
ISSN (online)	1873-2534
ISSN	0165-2427
DOI	10.1016/j.vetimm.2019.109939
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2177: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Middle East respiratory syndrome coronavirus infection in camelids.

Te, Nigeer / Ciurkiewicz, Malgorzata / van den Brand, Judith M A / Rodon, Jordi / Haverkamp, Ann-Kathrin / Vergara-Alert, Júlia / Bensaid, Albert / Haagmans, Bart L / Baumgartner, Wolfgang / Segalés, Joaquim

Veterinary pathology

2022 Volume 59, Issue 4, Page(s) 546–555

Abstract	Middle East respiratory syndrome coronavirus (MERS-CoV) is the cause of a severe respiratory disease with a high case fatality rate in humans. Since its emergence in mid-2012, 2578 laboratory-confirmed cases in 27 countries have been reported by the World Health Organization, leading to 888 known deaths due to the disease and related complications. Dromedary camels are considered the major reservoir host for this virus leading to zoonotic infection in humans. Dromedary camels, llamas, and alpacas are susceptible to MERS-CoV, developing a mild-to-moderate upper respiratory tract infection characterized by epithelial hyperplasia as well as infiltration of neutrophils, lymphocytes, and some macrophages within epithelium, lamina propria, in association with abundant viral antigen. The very mild lesions in the lower respiratory tract of these camelids correlate with absence of overt illness following MERS-CoV infection. Unfortunately, there is no approved antiviral treatment or vaccine for MERS-CoV infection in humans. Thus, there is an urgent need to develop intervention strategies in camelids, such as vaccination, to minimize virus spillover to humans. Therefore, the development of camelid models of MERS-CoV infection is key not only to assess vaccine prototypes but also to understand the biologic mechanisms by which the infection can be naturally controlled in these reservoir species. This review summarizes information on virus-induced pathological changes, pathogenesis, viral epidemiology, and control strategies in camelids, as the intermediate hosts and primary source of MERS-CoV infection in humans.
MeSH term(s)	Animals ; Camelids, New World ; Camelus ; Coronavirus Infections/epidemiology ; Coronavirus Infections/veterinary ; Middle East Respiratory Syndrome Coronavirus ; Zoonoses
Language	English
Publishing date	2022-01-08
Publishing country	United States
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	188012-3
ISSN	1544-2217 ; 0300-9858
ISSN (online)	1544-2217
ISSN	0300-9858
DOI	10.1177/03009858211069120
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 629: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein.

Haverkamp, Ann-Kathrin / Bosch, Berend J / Spitzbarth, Ingo / Lehmbecker, Annika / Te, Nigeer / Bensaid, Albert / Segalés, Joaquim / Baumgärtner, Wolfgang

Veterinary immunology and immunopathology

2019 Volume 218, Page(s) 109939

Abstract	Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.
MeSH term(s)	Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Viral/immunology ; Antigens, Viral/immunology ; Camelids, New World/virology ; Epitopes/immunology ; Formaldehyde ; Humans ; Immunohistochemistry ; Middle East Respiratory Syndrome Coronavirus/chemistry ; Middle East Respiratory Syndrome Coronavirus/immunology ; Nose/virology ; Paraffin Embedding ; Prospective Studies ; Spike Glycoprotein, Coronavirus/immunology
Chemical Substances	Antibodies, Monoclonal ; Antibodies, Viral ; Antigens, Viral ; Epitopes ; Spike Glycoprotein, Coronavirus ; Formaldehyde (1HG84L3525)
Keywords	covid19
Language	English
Publishing date	2019-09-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	754160-0
ISSN	1873-2534 ; 0165-2427
ISSN (online)	1873-2534
ISSN	0165-2427
DOI	10.1016/j.vetimm.2019.109939
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2177: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections.

Störk, Theresa / de le Roi, Madeleine / Haverkamp, Ann-Kathrin / Jesse, Sonja T / Peters, Martin / Fast, Christine / Gregor, Katharina M / Könenkamp, Laura / Steffen, Imke / Ludlow, Martin / Beineke, Andreas / Hansmann, Florian / Wohlsein, Peter / Osterhaus, Albert D M E / Baumgärtner, Wolfgang

Scientific reports

2021 Volume 11, Issue 1, Page(s) 24191

Abstract: Usutu virus (USUV) is a zoonotic arbovirus causing avian mass mortalities. The first outbreak in North-Western Germany occurred in 2018. This retrospective analysis focused on combining virological and pathological findings in birds and ... ...

Abstract	Usutu virus (USUV) is a zoonotic arbovirus causing avian mass mortalities. The first outbreak in North-Western Germany occurred in 2018. This retrospective analysis focused on combining virological and pathological findings in birds and immunohistochemistry. 25 common blackbirds, one great grey owl, and one kingfisher collected from 2011 to 2018 and positive for USUV by qRT-PCR were investigated. Macroscopically, most USUV infected birds showed splenomegaly and hepatomegaly. Histopathological lesions included necrosis and lymphohistiocytic inflammation within spleen, Bursa fabricii, liver, heart, brain, lung and intestine. Immunohistochemistry revealed USUV antigen positive cells in heart, spleen, pancreas, lung, brain, proventriculus/gizzard, Bursa fabricii, kidney, intestine, skeletal muscle, and liver. Analysis of viral genome allocated the virus to Europe 3 or Africa 2 lineage. This study investigated whether immunohistochemical detection of double-stranded ribonucleic acid (dsRNA) serves as an alternative tool to detect viral intermediates. Tissue samples of six animals with confirmed USUV infection by qRT-PCR but lacking viral antigen in liver and spleen, were further examined immunohistochemically. Two animals exhibited a positive signal for dsRNA. This could indicate either an early state of infection without sufficient formation of virus translation products, occurrence of another concurrent virus infection or endogenous dsRNA not related to infectious pathogens and should be investigated in more detail in future studies.
MeSH term(s)	Animals ; Bird Diseases/genetics ; Brain ; Disease Outbreaks ; Flavivirus/genetics ; Flavivirus Infections/genetics ; Genome, Viral ; Germany ; Heart ; History, 21st Century ; Immunohistochemistry ; Lung ; Pancreas ; Phylogeny ; Retrospective Studies ; Songbirds/metabolism ; Spleen ; Strigiformes/metabolism
Language	English
Publishing date	2021-12-17
Publishing country	England
Document type	Historical Article ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-03638-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Computer-assisted mitotic count using a deep learning-based algorithm improves interobserver reproducibility and accuracy

Bertram, Christof A. / Aubreville, Marc / Donovan, Taryn A. / Bartel, Alexander / Wilm, Frauke / Marzahl, Christian / Assenmacher, Charles-Antoine / Becker, Kathrin / Bennett, Mark / Corner, Sarah / Cossic, Brieuc / Denk, Daniela / Dettwiler, Martina / Gonzalez, Beatriz Garcia / Gurtner, Corinne / Haverkamp, Ann-Kathrin / Heier, Annabelle / Lehmbecker, Annika / Merz, Sophie /

Noland, Erica L. / Plog, Stephanie / Schmidt, Anja / Sebastian, Franziska / Sledge, Dodd G. / Smedley, Rebecca C. / Tecilla, Marco / Thaiwong, Tuddow / Fuchs-Baumgartinger, Andrea / Meuten, Donald J. / Breininger, Katharina / Kiupel, Matti / Maier, Andreas / Klopfleisch, Robert

Veterinary Pathology. 2022 Mar., v. 59, no. 2 p.211-226

2022

Abstract: The mitotic count (MC) is an important histological parameter for prognostication of malignant neoplasms. However, it has inter- and intraobserver discrepancies due to difficulties in selecting the region of interest (MC-ROI) and in identifying or ... ...

Abstract	The mitotic count (MC) is an important histological parameter for prognostication of malignant neoplasms. However, it has inter- and intraobserver discrepancies due to difficulties in selecting the region of interest (MC-ROI) and in identifying or classifying mitotic figures (MFs). Recent progress in the field of artificial intelligence has allowed the development of high-performance algorithms that may improve standardization of the MC. As algorithmic predictions are not flawless, computer-assisted review by pathologists may ensure reliability. In the present study, we compared partial (MC-ROI preselection) and full (additional visualization of MF candidates and display of algorithmic confidence values) computer-assisted MC analysis to the routine (unaided) MC analysis by 23 pathologists for whole-slide images of 50 canine cutaneous mast cell tumors (ccMCTs). Algorithmic predictions aimed to assist pathologists in detecting mitotic hotspot locations, reducing omission of MFs, and improving classification against imposters. The interobserver consistency for the MC significantly increased with computer assistance (interobserver correlation coefficient, ICC = 0.92) compared to the unaided approach (ICC = 0.70). Classification into prognostic stratifications had a higher accuracy with computer assistance. The algorithmically preselected hotspot MC-ROIs had a consistently higher MCs than the manually selected MC-ROIs. Compared to a ground truth (developed with immunohistochemistry for phosphohistone H3), pathologist performance in detecting individual MF was augmented when using computer assistance (F1-score of 0.68 increased to 0.79) with a reduction in false negatives by 38%. The results of this study demonstrate that computer assistance may lead to more reproducible and accurate MCs in ccMCTs.
Keywords	algorithms ; animal pathology ; artificial intelligence ; computers ; dogs ; immunohistochemistry ; mast cells ; mitosis ; canine cutaneous mast cell tumors ; digital pathology ; deep learning ; mitotic figures ; mitotic count ; automated image analysis ; computer assistance
Language	English
Dates of publication	2022-03
Size	p. 211-226.
Publishing place	SAGE Publications
Document type	Article ; Online
ZDB-ID	188012-3
ISSN	1544-2217 ; 0300-9858
ISSN (online)	1544-2217
ISSN	0300-9858
DOI	10.1177/03009858211067478
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 1959: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Comparison of Different

Pfankuche, Vanessa M / Hahn, Kerstin / Bodewes, Rogier / Hansmann, Florian / Habierski, André / Haverkamp, Ann-Kathrin / Pfaender, Stephanie / Walter, Stephanie / Baechlein, Christine / Postel, Alexander / Steinmann, Eike / Becher, Paul / Osterhaus, Albert / Baumgärtner, Wolfgang / Puff, Christina

Viruses

2018 Volume 10, Issue 7

Abstract: ... In ... ...

Abstract	In situ
MeSH term(s)	Animals ; Cattle/virology ; DNA Viruses/genetics ; DNA Viruses/isolation & purification ; DNA, Viral/genetics ; Dogs/virology ; Horses/virology ; In Situ Hybridization, Fluorescence/methods ; Liver/virology ; Lung/virology ; Lymph Nodes/virology ; RNA Probes ; RNA Viruses/genetics ; RNA Viruses/isolation & purification ; RNA, Viral/genetics ; Swine/virology
Chemical Substances	DNA, Viral ; RNA Probes ; RNA, Viral
Language	English
Publishing date	2018-07-20
Publishing country	Switzerland
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v10070384
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito