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  1. Book: Flow cytometry protocols

    Hawley, Teresa S. / Hawley, Robert G.

    (Methods in molecular biology ; 2779 ; Springer protocols)

    2024  

    Author's details edited by Teresa S. Hawley, Robert G. Hawley
    Series title Methods in molecular biology ; 2779
    Springer protocols
    Collection
    Language English
    Size xv, 458 Seiten, Illustrationen
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT030726877
    ISBN 9781071637371 ; 1071637371
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
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  2. Book: Flow cytometry protocols

    Hawley, Robert G. / Hawley, Teresa S.

    (Methods in molecular biology ; 1678 ; Springer protocols ; Chemistry)

    2018  

    Author's details edited by Teresa S. Hawley (Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA), Robert G. Hawley (Department of Anatomy and Regenerative Biology, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA)
    Series title Methods in molecular biology ; 1678
    Springer protocols
    Chemistry
    Collection
    Keywords intracellular protein biomarkers ; cytokine staining ; apoptosis analysis ; cell cycle ; FRET ; antibodies ; proteins
    Subject code 570
    Language English
    Size xiv, 492 Seiten, Illustrationen, Diagramme, 25.4 cm x 17.8 cm
    Edition Fourth edition
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT019480557
    ISBN 978-1-4939-7344-6 ; 1-4939-7344-4 ; 9781493973460 ; 1493973460
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

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    Zs A 7042 -1678- verfügbar in Königswinter Königswinter

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  3. Article ; Online: CRISPR-Cas9-Mediated Bioluminescent Tagging of Endogenous Proteins by Fluorescent Protein-Assisted Cell Sorting.

    Hawley, Robert G / Hawley, Teresa S

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2779, Page(s) 273–286

    Abstract: Oncogenic fusion genes are attractive therapeutic targets because of their tumor-specific expression and central "driver" roles in various human cancers. However, oncogenic fusions involving transcription factors such as PAX3-FOXO1 in alveolar fusion ... ...

    Abstract Oncogenic fusion genes are attractive therapeutic targets because of their tumor-specific expression and central "driver" roles in various human cancers. However, oncogenic fusions involving transcription factors such as PAX3-FOXO1 in alveolar fusion gene-positive rhabdomyosarcoma (FP-RMS) have been difficult to inhibit due to the apparent lack of tractable drug-like binding sites comparable to that recognized by Gleevec (imatinib mesylate) on the BCR-ABL1 tyrosine kinase fusion protein. Toward the identification of novel small molecules that selectively target PAX3-FOXO1, we used CRISPR-Cas9-mediated knock-in to append the pro-luminescent HiBiT tag onto the carboxy terminus of the endogenous PAX3-FOXO1 fusion protein in two human FP-RMS cell lines (RH4 and SCMC). HiBiT is an 11-amino acid peptide derived from the NanoLuc luciferase that produces a luminescence signal which is ~100-fold brighter than firefly or Renilla luciferases through high-affinity binding to a complementary NanoLuc peptide fragment called LgBiT. To facilitate single-cell clonal isolation of knock-ins, the homology-directed repair template encoding HiBiT was followed by a P2A self-cleaving peptide for coexpression of an mCherry fluorescent protein as a fluorescence-activated cell sorter (FACS)-selectable marker. HiBiT tagging thus allows highly sensitive luminescence detection of endogenous PAX3-FOXO1 levels permitting quantitative high-throughput screening of large compound libraries for the discovery of PAX3-FOXO1 inhibitors and degraders.
    MeSH term(s) Humans ; Paired Box Transcription Factors/genetics ; Paired Box Transcription Factors/metabolism ; CRISPR-Cas Systems ; Rhabdomyosarcoma/genetics ; Peptides/metabolism ; Oncogene Proteins, Fusion/genetics ; Oncogene Proteins, Fusion/metabolism ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Red Fluorescent Protein
    Chemical Substances Paired Box Transcription Factors ; mCherry fluorescent protein ; Peptides ; Oncogene Proteins, Fusion ; Red Fluorescent Protein
    Language English
    Publishing date 2024-04-01
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3738-8_12
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book: Flow cytometry protocols

    Hawley, Teresa S. / Hawley, Robert G.

    (Methods in molecular biology ; 699 ; Springer protocols ; Life sciences)

    2011  

    Author's details ed. by Teresa S. Hawley ; Robert G. Hawley
    Series title Methods in molecular biology ; 699
    Springer protocols
    Life sciences
    Collection
    Keywords Flow Cytometry / methods
    Language English
    Size XII, 485 S. : Ill., graph. Darst.
    Edition 3. ed.
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    HBZ-ID HT016570518
    ISBN 978-1-61737-949-9 ; 9781617379505 ; 1-61737-949-2 ; 1617379506
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2011 A 94 order for loan Magazin

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  5. Article: Correlating Chemical Sensitivity with Low Level Activation of Mechanotransduction Pathways in Hematologic Malignancies.

    Hawley, Robert G

    Exploratory research and hypothesis in medicine

    2017  Volume 2, Issue 3, Page(s) 63–67

    Abstract: Large-scale screening has revealed that human hematopoietic cancer cell lines are generally more sensitive to various classes of drugs than cell lines established from solid tumors. A detailed examination of data in the Cancer Therapeutics Response ... ...

    Abstract Large-scale screening has revealed that human hematopoietic cancer cell lines are generally more sensitive to various classes of drugs than cell lines established from solid tumors. A detailed examination of data in the Cancer Therapeutics Response Portal (http://portals.broadinstitute.org/ctrp/) suggests that this enhanced sensitivity is due to lower basal levels of activation of TAZ-TEAD mechanotransduction pathways in hematopoietic versus non-hematopoietic cells. Translation inhibitors such as omacetaxine mepesuccinate (homoharringtonine) fall into this category of hematopoietic-selective compounds. Moreover, additional molecular determinants of sensitivity suggest that homoharringtonine might show therapeutic efficacy in certain patients with advanced hematologic malignancies despite activation of these pathways.
    Language English
    Publishing date 2017-09-11
    Publishing country United States
    Document type Journal Article ; Comment
    ISSN 2472-0712
    ISSN 2472-0712
    DOI 10.14218/ERHM.2017.00022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Increased expression of the tight junction protein TJP1/ZO-1 is associated with upregulation of TAZ-TEAD activity and an adult tissue stem cell signature in carfilzomib-resistant multiple myeloma cells and high-risk multiple myeloma patients.

    Riz, Irene / Hawley, Robert G

    Oncoscience

    2017  Volume 4, Issue 7-8, Page(s) 79–94

    Abstract: Tight junction protein 1 (TJP1) has recently been proposed as a biomarker to identify multiple myeloma (MM) patients most likely to respond to bortezomib- and carfilzomib-based proteasome inhibitor regimens. Herein we report increased expression ... ...

    Abstract Tight junction protein 1 (TJP1) has recently been proposed as a biomarker to identify multiple myeloma (MM) patients most likely to respond to bortezomib- and carfilzomib-based proteasome inhibitor regimens. Herein we report increased expression of
    Language English
    Publishing date 2017-08-01
    Publishing country United States
    Document type Journal Article
    ISSN 2331-4737
    ISSN 2331-4737
    DOI 10.18632/oncoscience.356
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Nrf2: not "lost in translation".

    Hawley, Robert G / Riz, Irene

    Aging

    2016  Volume 8, Issue 12, Page(s) 3153–3154

    Language English
    Publishing date 2016--28
    Publishing country United States
    Document type Journal Article
    ISSN 1945-4589
    ISSN (online) 1945-4589
    DOI 10.18632/aging.101152
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Neopetrotaurines A-C, Isoquinoline Alkaloids with an Unprecedented Taurine Bridge from the Sponge

    Wang, Dongdong / Jiang, Wei / Churiwal, Mehal / Jia, Katrina / Senadeera, Sarath P D / Bokesch, Heidi R / Woldemichael, Girma M / Kim, Yong / Hawley, Robert G / Wei, Jun S / Khan, Javed / O'Keefe, Barry R / Beutler, John A / Gustafson, Kirk R

    Journal of natural products

    2024  Volume 87, Issue 2, Page(s) 332–339

    Abstract: Neopetrotaurines A-C ( ...

    Abstract Neopetrotaurines A-C (
    MeSH term(s) Animals ; Porifera ; Rhabdomyosarcoma, Alveolar/metabolism ; Cell Line ; Alkaloids/pharmacology ; Isoquinolines/pharmacology
    Chemical Substances Alkaloids ; Isoquinolines
    Language English
    Publishing date 2024-01-31
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Intramural
    ZDB-ID 304325-3
    ISSN 1520-6025 ; 0163-3864
    ISSN (online) 1520-6025
    ISSN 0163-3864
    DOI 10.1021/acs.jnatprod.3c01041
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1144: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Does retroviral insertional mutagenesis play a role in the generation of induced pluripotent stem cells?

    Hawley, Robert G

    Molecular therapy : the journal of the American Society of Gene Therapy

    2008  Volume 16, Issue 8, Page(s) 1354–1355

    MeSH term(s) Animals ; Host-Pathogen Interactions ; Mice ; Mutagenesis, Insertional ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism ; Pluripotent Stem Cells/virology ; Retroviridae/genetics ; Retroviridae/physiology
    Language English
    Publishing date 2008-07-25
    Publishing country United States
    Document type Letter ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2010592-7
    ISSN 1525-0024 ; 1525-0016
    ISSN (online) 1525-0024
    ISSN 1525-0016
    DOI 10.1038/mt.2008.142
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5640: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Fluorescent Proteins for Flow Cytometry.

    Hawley, Teresa S / Hawley, Robert G / Telford, William G

    Current protocols in cytometry

    2017  Volume 80, Page(s) 9.12.1–9.12.20

    Abstract: Fluorescent proteins have become standard tools for cell and molecular biologists. The color palette of fluorescent proteins spans the ultraviolet, visible, and near-infrared spectrum. Utility of fluorescent proteins has been greatly facilitated by the ... ...

    Abstract Fluorescent proteins have become standard tools for cell and molecular biologists. The color palette of fluorescent proteins spans the ultraviolet, visible, and near-infrared spectrum. Utility of fluorescent proteins has been greatly facilitated by the availability of compact and affordable solid state lasers capable of providing various excitation wavelengths. In theory, the plethora of fluorescent proteins and lasers make it easy to detect multiple fluorescent proteins simultaneously. However, in practice, heavy spectral overlap due to broad excitation and emission spectra presents a challenge. In conventional flow cytometry, careful selection of excitation wavelengths and detection filters is necessary. Spectral flow cytometry, an emerging methodology that is not confined by the "one color, one detector" paradigm, shows promise in the facile detection of multiple fluorescent proteins. This chapter provides a synopsis of fluorescent protein development, a list of commonly used fluorescent proteins, some practical considerations and strategies for detection, and examples of applications. © 2017 by John Wiley & Sons, Inc.
    MeSH term(s) Biosensing Techniques ; Biotinylation ; Flow Cytometry/methods ; Genetic Vectors/metabolism ; Lasers ; Luminescent Proteins/metabolism ; Protein Interaction Mapping
    Chemical Substances Luminescent Proteins
    Language English
    Publishing date 2017-04-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2179050-4
    ISSN 1934-9300 ; 1934-9297
    ISSN (online) 1934-9300
    ISSN 1934-9297
    DOI 10.1002/cpcy.17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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