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2024
Abstract: ... ...
| Abstract | Most |
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| Language | English |
| Publishing date | 2024-02-13 |
| Publishing country | United States |
| Document type | Preprint |
| DOI | 10.21203/rs.3.rs-3888175/v1 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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PLoS neglected tropical diseases
2022 Volume 16, Issue 12, Page(s) e0010991
Abstract: Plasmodium vivax infections often consist of heterogenous populations of parasites at different developmental stages and with distinct transcriptional profiles, which complicates gene expression analyses. The advent of single cell RNA sequencing (scRNA- ... ...
| Abstract | Plasmodium vivax infections often consist of heterogenous populations of parasites at different developmental stages and with distinct transcriptional profiles, which complicates gene expression analyses. The advent of single cell RNA sequencing (scRNA-seq) enabled disentangling this complexity and has provided robust and stage-specific characterization of Plasmodium gene expression. However, scRNA-seq information is typically derived from the end of each mRNA molecule (usually the 3'-end) and therefore fails to capture the diversity in transcript isoforms documented in bulk RNA-seq data. Here, we describe the sequencing of scRNA-seq libraries using Pacific Biosciences (PacBio) chemistry to characterize full-length Plasmodium vivax transcripts from single cell parasites. Our results show that many P. vivax genes are transcribed into multiple isoforms, primarily through variations in untranslated region (UTR) length or splicing, and that the expression of many isoforms is developmentally regulated. Our findings demonstrate that long read sequencing can be used to characterize mRNA molecules at the single cell level and provides an additional resource to better understand the regulation of gene expression throughout the Plasmodium life cycle. |
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| MeSH term(s) | Humans ; Plasmodium vivax/genetics ; Protein Isoforms/genetics ; Gene Expression Profiling ; RNA-Seq ; Malaria, Vivax ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Transcriptome ; Sequence Analysis, RNA/methods ; High-Throughput Nucleotide Sequencing |
| Chemical Substances | Protein Isoforms ; RNA, Messenger |
| Language | English |
| Publishing date | 2022-12-16 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural |
| ZDB-ID | 2429704-5 |
| ISSN | 1935-2735 ; 1935-2735 |
| ISSN (online) | 1935-2735 |
| ISSN | 1935-2735 |
| DOI | 10.1371/journal.pntd.0010991 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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Medical sciences (Basel, Switzerland)
2020 Volume 8, Issue 4
Abstract: Pre-hospital treatment of traumatic brain injury (TBI) with co-existing polytrauma is complicated by requirements for intravenous fluid volume vs. hypotensive resuscitation. A low volume, small particle-size-oxygen-carrier perfluorocarbon emulsion NVX- ... ...
| Abstract | Pre-hospital treatment of traumatic brain injury (TBI) with co-existing polytrauma is complicated by requirements for intravenous fluid volume vs. hypotensive resuscitation. A low volume, small particle-size-oxygen-carrier perfluorocarbon emulsion NVX-428 (dodecafluoropentane emulsion; 2% |
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| Language | English |
| Publishing date | 2020-09-27 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2754473-4 |
| ISSN | 2076-3271 ; 2076-3271 |
| ISSN (online) | 2076-3271 |
| ISSN | 2076-3271 |
| DOI | 10.3390/medsci8040041 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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The journal of trauma and acute care surgery
2018 Volume 86, Issue 1, Page(s) 116–122
Abstract: Background: Aeromedical evacuation to definitive care is standard in current military conflicts. However, there is minimal knowledge on the effects of hypobaria (HYPO) on either the flight crew or patients. The effects of HYPO were investigated using ... ...
| Abstract | Background: Aeromedical evacuation to definitive care is standard in current military conflicts. However, there is minimal knowledge on the effects of hypobaria (HYPO) on either the flight crew or patients. The effects of HYPO were investigated using healthy swine. Methods: Anesthetized Yorkshire swine underwent a simulated 4 h "transport" to an altitude of 2,441 m (8,000 feet.; HYPO, N = 6) or at normobaric conditions (NORMO, N = 6). Physiologic and biochemical data were collected. Organ damage was assessed for hemorrhage, inflammation, edema, necrosis, and for lungs only, microatelectasis. Results: All parameters were similar prior to and after "transport" with no significant effects of HYPO on hemodynamic, neurologic, or oxygen transport parameters, nor on blood gas, chemistry, or complete blood count data. However, the overall Lung Injury Score was significantly worse in the HYPO than the NORMO group (10.78 ± 1.22 vs. 2.31 ± 0.71, respectively) with more edema/fibrin/hemorrhage in the subpleural, interlobular and alveolar space, more congestion in alveolar septa, and evidence of microatelectasis (vs. no microatelectasis in the NORMO group). There was also increased severity of pulmonary neutrophilic (1.69 ± 0.20 vs. 0.19 ± 0.13) and histiocytic inflammation (1.83 ± 0.23 vs. 0.47 ± 0.17) for HYPO versus NORMO, respectively. On the other hand, there was increased renal inflammation in NORMO compared with HYPO (1.00 ± 0.13 vs. 0.33 ± 0.17, respectively). There were no histopathological differences in brain (whole or individual regions), liver, pancreas, or adrenals. Conclusion: Hypobaria, itself, may have an adverse effect on the respiratory system, even in healthy individuals, and this may be superimposed on combat casualties where there may be preexisting lung injury. The additional effects of anesthesia and controlled ventilation on these results are unknown, and further studies are indicated using awake models to better characterize the mechanisms for this pathology and the factors that influence its severity. |
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| MeSH term(s) | Air Ambulances/statistics & numerical data ; Altitude ; Animals ; Atmospheric Pressure ; Barotrauma/complications ; Blood Gas Analysis/methods ; Brain/pathology ; Brain Injuries/etiology ; Disease Models, Animal ; Edema/pathology ; Female ; Hemodynamics/physiology ; Hemorrhage/pathology ; Inflammation/immunology ; Inflammation/pathology ; Lung/pathology ; Lung Injury/etiology ; Male ; Necrosis/pathology ; Pulmonary Atelectasis/pathology ; Swine |
| Language | English |
| Publishing date | 2018-07-06 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. |
| ZDB-ID | 2651070-4 |
| ISSN | 2163-0763 ; 2163-0755 |
| ISSN (online) | 2163-0763 |
| ISSN | 2163-0755 |
| DOI | 10.1097/TA.0000000000002014 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 127: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
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The journal of trauma and acute care surgery
2016 Volume 81, Issue 1, Page(s) 101–107
Abstract: Background: There is inadequate information on the physiologic effects of aeromedical evacuation on wounded war fighters with traumatic brain injury (TBI). At altitudes of 8,000 ft, the inspired oxygen is lower than standard sea level values. In troops ... ...
| Abstract | Background: There is inadequate information on the physiologic effects of aeromedical evacuation on wounded war fighters with traumatic brain injury (TBI). At altitudes of 8,000 ft, the inspired oxygen is lower than standard sea level values. In troops experiencing TBI, this reduced oxygen may worsen or cause secondary brain injury. We tested the hypothesis that the effects of prolonged aeromedical evacuation on critical neurophysiologic parameters (i.e., brain oxygenation [PbtO2]) of swine with a fluid percussion injury/TBI would be detrimental compared with ground (normobaric) transport. Methods: Yorkshire swine underwent fluid percussion injury/TBI with pretransport stabilization before being randomized to a 4-hour aeromedical transport at simulated flight altitude of 8,000 ft (HYPO, n = 8) or normobaric ground transport (NORMO, n = 8). Physiologic measurements (i.e., PbtO2, cerebral perfusion pressure, intracranial pressure, regional cerebral blood flow, mean arterial blood pressure, and oxygen transport variables) were analyzed. Results: Survival was equivalent between groups. Measurements were similar in both groups at all phases up to and including onset of flight. During the flight, PbtO2, cerebral perfusion pressure, and mean arterial blood pressure were significantly lower in the HYPO than in the NORMO group. At the end of flight, regional cerebral blood flow was lower in the HYPO than in the NORMO group. Other parameters such as intracranial pressure, cardiac output, and mean pulmonary artery pressure were not significantly different between the two groups. Conclusion: A 4-hour aeromedical evacuation at a simulated flight altitude of 8,000 ft caused a notable reduction in neurophysiologic parameters compared with normobaric conditions in this TBI swine model. Results suggest that hypobaric conditions exacerbate cerebral hypoxia and may worsen TBI in casualties already in critical condition. |
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| MeSH term(s) | Air Ambulances ; Altitude ; Animals ; Brain Injuries, Traumatic/mortality ; Brain Injuries, Traumatic/physiopathology ; Cardiac Output ; Cerebrovascular Circulation ; Disease Models, Animal ; Hypoxia, Brain/mortality ; Hypoxia, Brain/physiopathology ; Intracranial Pressure ; Oxygen/blood ; Random Allocation ; Survival Rate ; Swine |
| Chemical Substances | Oxygen (S88TT14065) |
| Language | English |
| Publishing date | 2016-07 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2651070-4 |
| ISSN | 2163-0763 ; 2163-0755 |
| ISSN (online) | 2163-0763 |
| ISSN | 2163-0755 |
| DOI | 10.1097/TA.0000000000001048 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 127: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
2016 Volume 1634, Page(s) 132–139
Abstract: Background: Hypoxia is a critical secondary injury mechanism in traumatic brain injury (TBI), and early intervention to alleviate post-TBI hypoxia may be beneficial. NVX-108, a dodecafluoropentane perfluorocarbon, was screened for its ability to ... ...
| Abstract | Background: Hypoxia is a critical secondary injury mechanism in traumatic brain injury (TBI), and early intervention to alleviate post-TBI hypoxia may be beneficial. NVX-108, a dodecafluoropentane perfluorocarbon, was screened for its ability to increase brain tissue oxygen tension (PbtO2) when administered soon after TBI. Methods: Ketamine-acepromazine anesthetized rats ventilated with 40% oxygen underwent moderate controlled cortical impact (CCI)-TBI at time 0 (T0). Rats received either no treatment (NON, n=8) or 0.5 ml/kg intravenous (IV) NVX-108 (NVX, n=9) at T15 (15 min after TBI) and T75. Results: Baseline cortical PbtO2 was 28±3 mm Hg and CCI-TBI resulted in a 46±6% reduction in PbtO2 at T15 (P<0.001). Significant differences in time-group interactions (P=0.013) were found when comparing either absolute or percentage change of PbtO2 to post-injury (mixed-model ANOVA) suggesting that administration of NVX-108 increased PbtO2 above injury levels while it remained depressed in the NON group. Specifically in the NVX group, PbtO2 increased to a peak 143% of T15 (P=0.02) 60 min after completion of NVX-108 injection (T135). Systemic blood pressure was not different between the groups. Conclusion: NVX-108 caused an increase in PbtO2 following CCI-TBI in rats and should be evaluated further as a possible immediate treatment for TBI. |
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| MeSH term(s) | Animals ; Blood Pressure/drug effects ; Brain Injuries, Traumatic/complications ; Brain Injuries, Traumatic/metabolism ; Cerebral Cortex/drug effects ; Cerebral Cortex/injuries ; Cerebral Cortex/metabolism ; Fluorocarbons/administration & dosage ; Heart Rate/drug effects ; Hypoxia/etiology ; Hypoxia/metabolism ; Hypoxia/prevention & control ; Male ; Partial Pressure ; Rats ; Rats, Sprague-Dawley |
| Chemical Substances | Fluorocarbons ; perfluoropentane (678-26-2) |
| Language | English |
| Publishing date | 2016-03-01 |
| Publishing country | Netherlands |
| Document type | Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. |
| ZDB-ID | 1200-2 |
| ISSN | 1872-6240 ; 0006-8993 |
| ISSN (online) | 1872-6240 |
| ISSN | 0006-8993 |
| DOI | 10.1016/j.brainres.2016.01.012 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 161: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.