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  1. Article: Extracellular Vesicles as Potential Biomarkers for Early Detection and Diagnosis of Pancreatic Cancer.

    Yee, Nelson S / Zhang, Sheng / He, Hong-Zhang / Zheng, Si-Yang

    Biomedicines

    2020  Volume 8, Issue 12

    Abstract: Pancreatic carcinoma (PC) is highly metastatic, and it tends to be detected at advanced stages. Identifying and developing biomarkers for early detection of PC is crucial for a potentially curative treatment. Extracellular vesicles (EVs) are bilayer ... ...

    Abstract Pancreatic carcinoma (PC) is highly metastatic, and it tends to be detected at advanced stages. Identifying and developing biomarkers for early detection of PC is crucial for a potentially curative treatment. Extracellular vesicles (EVs) are bilayer lipid membrane-structured nanovesicles found in various human bodily fluids, and they play important roles in tumor biogenesis and metastasis. Cancer-derived EVs are enriched with DNA, RNA, protein, and lipid, and they have emerged as attractive diagnostic biomarkers for early detection of PC. In this article, we provided an overview of the cell biology of EVs and their isolation and analysis, and their roles in cancer pathogenesis and progression. Multiplatform analyses of plasma-based exosomes for genomic DNA, micro RNA, mRNA, circular RNA, and protein for diagnosis of PC were critically reviewed. Numerous lines of evidence demonstrate that liquid biopsy with analysis of EV-based biomarkers has variable performance for diagnosis of PC. Future investigation is indicated to optimize the methodology for isolating and analyzing EVs and to identify the combination of EV-based biomarkers and other clinical datasets, with the goal of improving the predictive value, sensitivity, and specificity of screening tests for early detection and diagnosis of PC.
    Language English
    Publishing date 2020-12-07
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines8120581
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: An Ultrafast One-Step Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Detection of SARS-CoV-2.

    Milosevic, Jadranka / Lu, Mengrou / Greene, Wallace / He, Hong-Zhang / Zheng, Si-Yang

    Frontiers in microbiology

    2021  Volume 12, Page(s) 749783

    Abstract: We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on ... ...

    Abstract We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop
    Language English
    Publishing date 2021-11-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2021.749783
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  3. Article ; Online: Nanoscale coordination polymers enabling antioxidants inhibition for enhanced chemodynamic therapy.

    Zhang, Yan / Wang, Faming / Shi, Lai / Lu, Mengrou / Lee, Keng-Jung / Ditty, Mackenzie Maurer / Xing, Yunhui / He, Hong-Zhang / Ren, Xi / Zheng, Si-Yang

    Journal of controlled release : official journal of the Controlled Release Society

    2023  Volume 354, Page(s) 196–206

    Abstract: Reactive oxygen species (ROS) generation to induce cell death is an effective strategy for cancer therapy. In particular, chemodynamic therapy (CDT), using Fenton-type reactions to generate highly cytotoxic hydroxyl radical (•OH), is a promising ... ...

    Abstract Reactive oxygen species (ROS) generation to induce cell death is an effective strategy for cancer therapy. In particular, chemodynamic therapy (CDT), using Fenton-type reactions to generate highly cytotoxic hydroxyl radical (•OH), is a promising treatment modality. However, the therapeutic efficacy of ROS-based cancer treatment is still limited by some critical challenges, such as overexpression of enzymatic and non-enzymatic antioxidants by tumor cells, as well as the low tumor targeting efficiency of therapeutic agents. To address those problems, biomimetic CuZn protoporphyrin IX nanoscale coordination polymers have been developed, which significantly amplify oxidative stress against tumors by simultaneously inhibiting enzymatic and non-enzymatic antioxidants and initiating the CDT. In this design, cancer cell membrane camouflaged nanoparticle exhibits an excellent homotypic targeting effect. After being endocytosed into tumor cells, the nanoparticles induce depletion of the main non-enzymatic antioxidant glutathione (GSH) by undergoing a redox reaction with GSH. Afterward, the redox reaction generated cuprous ion (Cu
    MeSH term(s) Humans ; Antioxidants ; Reactive Oxygen Species ; Glutathione ; Oxidative Stress ; Biomimetics ; Nanoparticles ; Cell Line, Tumor ; Neoplasms ; Hydrogen Peroxide ; Tumor Microenvironment
    Chemical Substances Antioxidants ; Reactive Oxygen Species ; Glutathione (GAN16C9B8O) ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2023-01-10
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 632533-6
    ISSN 1873-4995 ; 0168-3659
    ISSN (online) 1873-4995
    ISSN 0168-3659
    DOI 10.1016/j.jconrel.2023.01.004
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2055: Show issues Location:
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  4. Article ; Online: Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica.

    Wan, Yuan / Maurer, Mackenzie / He, Hong-Zhang / Xia, Yi-Qiu / Hao, Si-Jie / Zhang, Wen-Long / Yee, Nelson S / Zheng, Si-Yang

    Lab on a chip

    2019  Volume 19, Issue 14, Page(s) 2346–2355

    Abstract: Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation ...

    Abstract Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation platform that utilizes nEV size-matched silica nanostructures and a surface-conjugated lipid nanoprobe with an integrated microfluidic mixer. The reported platform has 28.8% capture efficiency from pancreatic cancer plasma and can sufficiently enrich nEVs for simpler positive identification of point mutations, particularly KRAS, in nEV DNA from the plasma of pancreatic cancer patients.
    MeSH term(s) Cell Line, Tumor ; Extracellular Vesicles/chemistry ; Extracellular Vesicles/pathology ; Feasibility Studies ; Humans ; Lab-On-A-Chip Devices ; Lipids/chemistry ; Mutation ; Nanostructures/chemistry ; Pancreatic Neoplasms/blood ; Pancreatic Neoplasms/genetics ; Pancreatic Neoplasms/pathology ; Proto-Oncogene Proteins p21(ras)/genetics ; Silicon Dioxide/chemistry
    Chemical Substances KRAS protein, human ; Lipids ; Silicon Dioxide (7631-86-9) ; Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2)
    Language English
    Publishing date 2019-07-01
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/c8lc01359d
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A colorimetric and luminescent dual-modal assay for Cu(II) ion detection using an iridium(III) complex.

    Ma, Dik-Lung / He, Hong-Zhang / Chan, Daniel Shiu-Hin / Wong, Chun-Yuen / Leung, Chung-Hang

    PloS one

    2014  Volume 9, Issue 6, Page(s) e99930

    Abstract: A novel iridium(III) complex-based chemosensor bearing the 5,6-bis(salicylideneimino)-1,10-phenanthroline ligand receptor was developed, which exhibited a highly sensitive and selective color change from colorless to yellow and a visible turn-off ... ...

    Abstract A novel iridium(III) complex-based chemosensor bearing the 5,6-bis(salicylideneimino)-1,10-phenanthroline ligand receptor was developed, which exhibited a highly sensitive and selective color change from colorless to yellow and a visible turn-off luminescence response upon the addition of Cu(II) ions. The interactions of this iridium(III) complex with Cu2+ ions and thirteen other cations have been investigated by UV-Vis absorption titration, emission titration, and 1H NMR titration.
    MeSH term(s) Colorimetry/methods ; Copper/analysis ; Copper/chemistry ; Iridium/chemistry ; Luminescent Measurements/methods
    Chemical Substances Iridium (44448S9773) ; Copper (789U1901C5)
    Language English
    Publishing date 2014-06-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0099930
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  6. Article: A highly selective G-quadruplex-based luminescent switch-on probe for the detection of gene deletion

    He, Hong-Zhang / Chan, Daniel Shiu-Hin / Leung, Chung-Hang / Ma, Dik-Lung

    Chemical communications. 2012 Aug. 28, v. 48, no. 76

    2012  

    Abstract: A G-quadruplex-based switch-on luminescence assay has been developed for the detection of gene deletion using a cyclometallated iridium(iii) complex as a G-quadruplex-selective probe. Upon hybridization with the target DNA, the two split G-quadruplex- ... ...

    Abstract A G-quadruplex-based switch-on luminescence assay has been developed for the detection of gene deletion using a cyclometallated iridium(iii) complex as a G-quadruplex-selective probe. Upon hybridization with the target DNA, the two split G-quadruplex-forming sequences assemble into a split G-quadruplex, which greatly enhances the luminescence emission of the iridium(iii) probe. The assay is simple and highly selective.
    Keywords DNA ; chemical communication ; chemical reactions ; gene deletion ; iridium ; luminescence
    Language English
    Dates of publication 2012-0828
    Size p. 9462-9464.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/c2cc32253f
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  7. Article: Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

    He, Hong-Zhang / Chan, Daniel Shiu-Hin / Leung, Chung-Hang / Leung, Ka-Ho / Ma, Dik-Lung / Wang, Wei

    Chemical communications. 2014 Apr. 22, v. 50, no. 40

    2014  

    Abstract: A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening ... ...

    Abstract A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(iii) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.
    Keywords chemical compounds ; chemical reactions ; enzyme activity ; luminescence ; screening
    Language English
    Dates of publication 2014-0422
    Size p. 5313-5315.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/c3cc47444e
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  8. Article ; Online: G-quadruplexes for luminescent sensing and logic gates.

    He, Hong-Zhang / Chan, Daniel Shiu-Hin / Leung, Chung-Hang / Ma, Dik-Lung

    Nucleic acids research

    2013  Volume 41, Issue 8, Page(s) 4345–4359

    Abstract: G-quadruplexes represent a versatile sensing platform for the construction of label-free molecular detection assays owing to their diverse structures that can be selectively recognized by G-quadruplex-specific luminescent probes. In this Survey and ... ...

    Abstract G-quadruplexes represent a versatile sensing platform for the construction of label-free molecular detection assays owing to their diverse structures that can be selectively recognized by G-quadruplex-specific luminescent probes. In this Survey and Summary, we highlight recent examples of the application of the label-free strategy for the development of G-quadruplex-based luminescent detection platforms with a view towards the potential application of tetraplex structures in the design of DNA logic gates.
    MeSH term(s) Aptamers, Nucleotide ; Computers, Molecular ; DNA/analysis ; Enzymes/analysis ; G-Quadruplexes ; Luminescent Agents/chemistry ; Metals/analysis ; Sulfhydryl Compounds/analysis
    Chemical Substances Aptamers, Nucleotide ; Enzymes ; Luminescent Agents ; Metals ; Sulfhydryl Compounds ; DNA (9007-49-2)
    Language English
    Publishing date 2013-02-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkt108
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    Zs.A 1067: Show issues Location:
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  9. Article ; Online: Bioactive luminescent transition-metal complexes for biomedical applications.

    Ma, Dik-Lung / He, Hong-Zhang / Leung, Ka-Ho / Chan, Daniel Shiu-Hin / Leung, Chung-Hang

    Angewandte Chemie (International ed. in English)

    2013  Volume 52, Issue 30, Page(s) 7666–7682

    Abstract: The serendipitous discovery of the anticancer drug cisplatin cemented medicinal inorganic chemistry as an independent discipline in the 1960s. Luminescent metal complexes have subsequently been widely applied for sensing, bio-imaging, and in organic ... ...

    Abstract The serendipitous discovery of the anticancer drug cisplatin cemented medicinal inorganic chemistry as an independent discipline in the 1960s. Luminescent metal complexes have subsequently been widely applied for sensing, bio-imaging, and in organic light-emitting diode applications. Transition-metal complexes possess a variety of advantages that make them suitable as therapeutics and as luminescent probes for biomolecules. It is thus highly desirable to develop new luminescent metal complexes that either interact with DNA through different binding modes or target alternative cellular machinery such as proteins as well as to provide a more effective means of monitoring disease progression. In this Review, we highlight recent examples of biologically active luminescent metal complexes that can target and probe a specific biomolecule, and offer insights into the future potential of these compounds for the investigation and treatment of human diseases.
    MeSH term(s) Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Coordination Complexes/chemistry ; Drug Design ; Humans ; Luminescent Agents/chemistry ; Luminescent Agents/therapeutic use ; Luminescent Measurements/methods ; Metals/chemistry ; Metals/metabolism
    Chemical Substances Antineoplastic Agents ; Coordination Complexes ; Luminescent Agents ; Metals
    Language English
    Publishing date 2013-07-22
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.201208414
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  10. Article: A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine

    He, Hong-Zhang / Wang, Modi / Chan, Daniel Shiu-Hin / Leung, Chung-Hang / Qiu, Jian-Wen / Ma, Dik-Lung

    Methods. 2013 Dec. 15, v. 64, no. 3

    2013  

    Abstract: A label-free G-quadruplex-based luminescent switch-on assay has been developed for the selective detection of micromolar histidine in aqueous solution. In this study, an iridium(III) complex was employed as a G-quadruplex-specific luminescent probe while ...

    Abstract A label-free G-quadruplex-based luminescent switch-on assay has been developed for the selective detection of micromolar histidine in aqueous solution. In this study, an iridium(III) complex was employed as a G-quadruplex-specific luminescent probe while a guanine-rich oligonucleotide (Pu27, 5′-TG₄AG₃TG₄AG₃TG₄A₂G₂-3′)/cupric ion (Cu²⁺) ensemble was employed as a recognition unit for histidine. The initial luminescence of the iridium(III) complex in the presence of G-quadruplex DNA is effectively quenched by Cu²⁺ ions due to the Cu²⁺-mediated unfolding of the G-quadruplex motif. The addition of histidine sequesters Cu²⁺ ions from the ensemble, thereby restoring the luminescence of the system. The assay could detect down to 1μM of histidine in aqueous media, and also exhibited good selectivity for histidine over other amino acids with the use of the cysteine, masking agent N-ethylmaleimide. Furthermore, the application of the assay for the detection of histidine in diluted urine samples was demonstrated.
    Keywords DNA ; aqueous solutions ; copper ; cysteine ; histidine ; ions ; iridium ; luminescence ; oligonucleotides ; urine
    Language English
    Dates of publication 2013-1215
    Size p. 205-211.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 1066584-5
    ISSN 1095-9130 ; 1046-2023
    ISSN (online) 1095-9130
    ISSN 1046-2023
    DOI 10.1016/j.ymeth.2013.07.023
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