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  1. Article ; Online: Purification, structural identification, antioxidant activity, and inhibitory effects on digestive enzymes of α-glucan from Chuanminshen violaceum

    Gao, Tao / Liu, Shuang / Luo, Huang-yang / Wu, Ren / Yan, Rui / He, Ling-zhi / Nie, Qing-yu / Tang, Hua-li / Sun, Guiju

    Journal of Functional Foods. 2023 May, v. 104 p.105536-

    2023  

    Abstract: Chuanminshen violaceum, as a food and folk medicine, is cultivated and grown only in China. Its polysaccharides play an important role in medicinal benefits. Herein, crude C. violaceum polysaccharides (CVPs) were purified to yield two polysaccharide ... ...

    Abstract Chuanminshen violaceum, as a food and folk medicine, is cultivated and grown only in China. Its polysaccharides play an important role in medicinal benefits. Herein, crude C. violaceum polysaccharides (CVPs) were purified to yield two polysaccharide fractions (CVPs-1-G and CVPs-2-G) using DEAE Cellulose-52 and Sephadex G-100 column chromatography. Structural analyses indicated that both CVPs-1-G and CVPs-2-G were glucans with average molecular weights of 8.1 kDa and 187.5 kDa, respectively. The main chain of the major component (CVPs-1-G) might be composed of (1 → 4)-linked α-Glcp, and the branched-chain might be an α-Glcp → 4)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 4)-α-D-Glcp, which connected to the main chain through the O-6 position of → 3,6)-α-D-Glcp-(1 →. In addition, CVPs-1-G displayed ABTS, DPPH, and hydroxyl radical scavenging activity, as well as competitive inhibitory activities of α-amylase and non-competitive inhibitory activities of lipase and pepsin. According to molecular docking, its inhibitory activity on digestive enzyme might be caused by hydrogen bond and hydrophobic interaction between them. Thus, CVPs exhibit strong potential for use in functional foods and pharmaceuticals.
    Keywords alpha-amylase ; antioxidant activity ; carboxylic ester hydrolases ; chromatography ; drugs ; glucans ; hydrogen bonding ; hydrophobic bonding ; hydroxyl radicals ; pepsin ; traditional medicine ; China ; Chuanminshen violaceum ; α-glucan ; Structural identification ; Digestive enzyme activity ; Kinetics of digestive enzyme
    Language English
    Dates of publication 2023-05
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 2511964-3
    ISSN 1756-4646
    ISSN 1756-4646
    DOI 10.1016/j.jff.2023.105536
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 7830: Show issues

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  2. Article ; Online: Optimization of Ultrasonic-Assisted Extraction of Total Flavonoids from Abrus Cantoniensis (

    Wu, En-Yun / Sun, Wen-Jing / Wang, Ying / Zhang, Ge-Yin / Xu, Bai-Chang / Chen, Xiao-Gang / Hao, Kai-Yuan / He, Ling-Zhi / Si, Hong-Bin

    Molecules (Basel, Switzerland)

    2022  Volume 27, Issue 7

    Abstract: Abrus cantoniensis is a Chinese herbal medicine with efficacy in clearing heat and detoxification, as well as relieving liver pain. The whole plant, except the seeds, can be used and consumed. Flavonoids have been found in modern pharmacological studies ... ...

    Abstract Abrus cantoniensis is a Chinese herbal medicine with efficacy in clearing heat and detoxification, as well as relieving liver pain. The whole plant, except the seeds, can be used and consumed. Flavonoids have been found in modern pharmacological studies to have important biological activities, such as anti-inflammatory, antibacterial and antioxidant properties. The antibacterial and antioxidant bioactivities of the total flavonoids of Abrus cantoniensis (ATF) have been widely reported in national and international journals, but there are fewer studies on their anti-inflammatory effects. The present study focused on the optimization of the ultrasonic extraction process of ATF by response surface methodology and the study of its anti-inflammatory effects in vitro and in vivo. The results showed that the factors that had a great impact on the ATF extraction were the material-to-liquid ratio, ultrasonic extraction cycles and ethanol concentration. The best extraction process used a material-to-liquid ratio of 1:47, ultrasonic extraction cycles of 4 times, an ethanol concentration of 50%, an ultrasonic extraction time of 40 min and an ultrasonic power of 125 W. Under these conditions, the actual extraction rate of total flavonoids was 3.68%, which was not significantly different from the predicted value of 3.71%. In an in vitro anti-inflammatory assay, ATF was found to be effective in alleviating LPS (lipopolysaccharide)-induced inflammation in mouse peritoneal macrophages. In an in vivo anti-inflammatory assay, ATF was found to have a significant inhibitory effect on xylene-induced ear swelling in mice and cotton ball granuloma in mice, and the inhibitory effect was close to that of the positive control drug dexamethasone. This may provide a theoretical basis for the further development of the medicinal value of Abrus cantoniensis.
    MeSH term(s) Abrus ; Animals ; Anti-Bacterial Agents ; Anti-Inflammatory Agents/pharmacology ; Antioxidants/pharmacology ; Ethanol ; Flavonoids/pharmacology ; Mice ; Plant Extracts/pharmacology ; Ultrasonics
    Chemical Substances Anti-Bacterial Agents ; Anti-Inflammatory Agents ; Antioxidants ; Flavonoids ; Plant Extracts ; Ethanol (3K9958V90M)
    Language English
    Publishing date 2022-03-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules27072036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 81: Show issues

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  3. Article: Total flavonoids of

    Sun, Wen-Jing / Wu, En-Yun / Zhang, Ge-Yin / Xu, Bai-Chang / Chen, Xiao-Gang / Hao, Kai-Yuan / Wang, Ying / He, Ling-Zhi / Lv, Qi-Zhuang

    Frontiers in microbiology

    2022  Volume 13, Page(s) 985529

    Abstract: Established a model of lipopolysaccharide (LPS)-induced mastitis in mice, pathological sections and myeloperoxidase were used to detect the degree of tissue damage, enzyme-linked immunosorbent assay (ELISA) was performed to detect the expression of pro- ... ...

    Abstract Established a model of lipopolysaccharide (LPS)-induced mastitis in mice, pathological sections and myeloperoxidase were used to detect the degree of tissue damage, enzyme-linked immunosorbent assay (ELISA) was performed to detect the expression of pro-inflammatory cytokines, meanwhile fluorescence quantitative PCR experiments were performed to detect the mRNA expression of CD14/TLR4/NF-κB/MAPK signalling pathway, and the faeces of mice were collected for 16S measurement of flora. The results showed that
    Language English
    Publishing date 2022-08-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.985529
    Database MEDical Literature Analysis and Retrieval System OnLINE

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