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  1. Article ; Online: Electrochemical aptasensor based on DNA-templated copper nanoparticles and RecJf exonuclease-assisted target recycling for lipopolysaccharide detection.

    Xie, Shunbi / Liang, Shuting / Tian, Liangliang / Ding, Ge / He, Meiting / Li, Haojie / Yang, Heshan

    Analytical methods : advancing methods and applications

    2024  Volume 16, Issue 3, Page(s) 396–402

    Abstract: An electrochemical aptasensor for detecting lipopolysaccharides (LPS) was fabricated based on DNA-templated copper nanoparticles (DNA-CuNPs) and RecJf exonuclease-assisted target recycling. The DNA-CuNPs were synthesized on a double-stranded DNA template ...

    Abstract An electrochemical aptasensor for detecting lipopolysaccharides (LPS) was fabricated based on DNA-templated copper nanoparticles (DNA-CuNPs) and RecJf exonuclease-assisted target recycling. The DNA-CuNPs were synthesized on a double-stranded DNA template generated through the hybridization of the LPS aptamer and its complementary chain (cDNA). In the absence of LPS, the CuNPs were synthesized on DNA double-strands, and a strong readout corresponding to the CuNPs was achieved at 0.10 V (
    MeSH term(s) Humans ; Exonucleases/chemistry ; Exonucleases/metabolism ; Lipopolysaccharides ; Copper/chemistry ; DNA, Complementary ; Reproducibility of Results ; Aptamers, Nucleotide/chemistry ; Aptamers, Nucleotide/metabolism ; Electrochemical Techniques ; Biosensing Techniques ; DNA/chemistry ; Metal Nanoparticles/chemistry
    Chemical Substances Exonucleases (EC 3.1.-) ; Lipopolysaccharides ; Copper (789U1901C5) ; DNA, Complementary ; Aptamers, Nucleotide ; DNA (9007-49-2)
    Language English
    Publishing date 2024-01-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2515210-5
    ISSN 1759-9679 ; 1759-9660
    ISSN (online) 1759-9679
    ISSN 1759-9660
    DOI 10.1039/d3ay01638b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Uncovering the Mechanism of Chinese Hawthorn Leaf on Myocardial Ischemia Based on Network Pharmacology, Molecular Docking Verification, and In Vitro Studies.

    Gao, Jingyun / Wang, Yueyue / Xiong, Hui / Zhao, Shengnan / He, Mingmei / He, Meiting / Pan, Haifeng

    Cardiovascular toxicology

    2024  Volume 24, Issue 2, Page(s) 171–183

    Abstract: Hawthorn leaf has shown therapeutic effects in the patients with myocardial ischemia. Our study combines network pharmacology, molecular docking techniques, and in vitro experiment with the aim of revealing the mechanism of hawthorn leaves in the ... ...

    Abstract Hawthorn leaf has shown therapeutic effects in the patients with myocardial ischemia. Our study combines network pharmacology, molecular docking techniques, and in vitro experiment with the aim of revealing the mechanism of hawthorn leaves in the treatment of myocardial ischemia. The active ingredients and corresponding targets of hawthorn leaf through Traditional Chinese Medicine System Pharmacology and Swiss Target Prediction databases. Targets related to myocardial ischemia were retrieved by Gene Card, Online Mendelian Inheritance in Man, Disgenet, and Therapeutic Targets Database databases. Cytoscape software was used to construct an ingredient-target-organ network and enrichment analysis of common targets was analyzed. Molecular docking verification of the core compound and target interactions was performed using MOE software. In vitro cell experiment was performed to verify the findings from bioinformatics analysis. Six active components and 107 potential therapeutic targets were screened. The protein-protein interaction network analysis indicated that 10 targets, including AKT1 and EGFR, were hub genes. Quercetin, kaempferol and isorhamnetin were taken as core active components. Through pathway enrichment analysis, nearly 455 Gene Ontology entries and 77 Kyoto Encyclopedia of Genes and Genomes pathways were obtained, mainly including PI3K/Akt, estrogen and other signaling pathways. Molecular docking prediction showed that three main active ingredients were firmly combined with the core targets. Cellular experiments showed that quercetin alleviated oxidative damage in cells and regulated the expression of PI3K, P-AKT/AKT and Bax/Bcl-2 proteins. This study identified the potential targets of Hawthorn leaf against myocardial ischemia using network pharmacology and in vitro verification, which provided a new understanding of the pharmacological mechanisms of Hawthorn leaf in treatment of myocardial ischemia.
    MeSH term(s) Humans ; Molecular Docking Simulation ; Crataegus ; Network Pharmacology ; Phosphatidylinositol 3-Kinases ; Proto-Oncogene Proteins c-akt ; Quercetin/pharmacology ; Coronary Artery Disease ; Myocardial Ischemia/drug therapy ; Myocardial Ischemia/genetics ; Databases, Genetic ; Drugs, Chinese Herbal/pharmacology
    Chemical Substances Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Quercetin (9IKM0I5T1E) ; Drugs, Chinese Herbal
    Language English
    Publishing date 2024-02-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2036765-X
    ISSN 1559-0259 ; 1530-7905
    ISSN (online) 1559-0259
    ISSN 1530-7905
    DOI 10.1007/s12012-024-09825-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5817: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Correction: Uncovering the Mechanism of Chinese Hawthorn Leaf on Myocardial Ischemia Based on Network Pharmacology, Molecular Docking Verification, and In Vitro Studies.

    Gao, Jingyun / Wang, Yueyue / Xiong, Hui / Zhao, Shengnan / He, Mingmei / He, Meiting / Pan, Haifeng

    Cardiovascular toxicology

    2024  Volume 24, Issue 6, Page(s) 622–623

    Language English
    Publishing date 2024-04-25
    Publishing country United States
    Document type Published Erratum
    ZDB-ID 2036765-X
    ISSN 1559-0259 ; 1530-7905
    ISSN (online) 1559-0259
    ISSN 1530-7905
    DOI 10.1007/s12012-024-09851-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5817: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Form Follows Environmental Energy

    HE Meiting / LI Linxue

    E3S Web of Conferences, Vol 101, p

    Ecological Heat In Contemporary Vernacular Architecture

    2019  Volume 02003

    Abstract: The aesthetic of architecture changes with the history, and its evolution is a dynamic, humane and regional process. The contemporary China is in the double transition periods of traditional and modern, modern and postmodern, therefore the value of ... ...

    Abstract The aesthetic of architecture changes with the history, and its evolution is a dynamic, humane and regional process. The contemporary China is in the double transition periods of traditional and modern, modern and postmodern, therefore the value of architecture lost its order caused by the overlapping and conflicting of different values in different periods, either external “image” or intrinsic “meaning”, are in a state of disorder. With the advent of modernization, traditional architecture seems to be gradually forgotten and abandoned. However, many traditional villages still circulate the environmental wisdom that contemporary architects still use in their designs. Most of the traditional houses all over the world use local building material, make full use of renewable energy, using the natural energy of natural climate actively such as light, heat, wind to adapt to the climate environment, with good adaptability to the local climate, topography and, is a model of passive building technology. This article attempts from Three angles to explore, which are the diagram and theory, simulation software, and environment measurement of Chinese traditional village. Through the above research, we try to find the coupling between traditional local-style dwelling houses and modern residence in the design.
    Keywords Environmental sciences ; GE1-350
    Subject code 720
    Language English
    Publishing date 2019-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Intranasal delivery of BDNF-loaded small extracellular vesicles for cerebral ischemia therapy.

    Zhou, Xin / Deng, Xiaohui / Liu, Mengfan / He, Meiting / Long, Wenhui / Xu, Zhibin / Zhang, Kun / Liu, Tao / So, Kwok-Fai / Fu, Qing-Ling / Zhou, Libing

    Journal of controlled release : official journal of the Controlled Release Society

    2023  Volume 357, Page(s) 1–19

    Abstract: Mesenchymal stem cells (MSCs) have shown promise for the therapy of cerebral ischemia in animal studies and clinical trials, yet their clinical application still faces many challenges. Utilizing small extracellular vesicles (sEVs) may overcome these ... ...

    Abstract Mesenchymal stem cells (MSCs) have shown promise for the therapy of cerebral ischemia in animal studies and clinical trials, yet their clinical application still faces many challenges. Utilizing small extracellular vesicles (sEVs) may overcome these challenges. In the study, we overexpressed brain-derived neurotrophic factor (BDNF) in cultured MSCs and purified sEVs using anion exchange chromatography. In an ischemic stroke mouse model, sEVs selectively targeted the peri-infarct region after intranasal administration, and BDNF loading enhanced the efficacy of sEVs in improved functional behavior, neural repair indicated by infarct volume reduction, increased neurogenesis, angiogenesis, synaptic plasticity, and fiber preservation, as well as decreased inflammatory-cytokine expression and glial response. Intranasal administration of sEVs and BDNF-sEVs resulted in upregulation of neuroprotection-related genes and downregulation of inflammation-related genes, and BDNF-sEVs treatment activated the BDNF/TrkB signaling in the ischemic brain. Transcriptomic and proteomic analysis of sEVs and BDNF-sEVs disclosed abundant proteins and miRNAs involved in neuroprotection and anti-inflammation, and BDNF-sEVs showed different characteristics from sEVs. In conclusion, intranasal delivery of sEVs-loaded BDNF is a promising alternative strategy for the therapy of cerebral ischemia.
    MeSH term(s) Mice ; Animals ; Brain-Derived Neurotrophic Factor/genetics ; Administration, Intranasal ; Proteomics ; Brain Ischemia/drug therapy ; Brain Ischemia/metabolism ; Infarction/drug therapy ; Extracellular Vesicles/metabolism
    Chemical Substances Brain-Derived Neurotrophic Factor
    Language English
    Publishing date 2023-03-28
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 632533-6
    ISSN 1873-4995 ; 0168-3659
    ISSN (online) 1873-4995
    ISSN 0168-3659
    DOI 10.1016/j.jconrel.2023.03.033
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2055: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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