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  1. Book: Pu tao xue

    He, Pu-chao

    (Dang dai ke ji zhong yao zhu zuo.)

    1999  

    Author's details He Pu-chao zhu bian
    Series title Dang dai ke ji zhong yao zhu zuo.
    Keywords Viticulture. ; Grape products. ; Wine and wine making.
    Language Chinese
    Size 4, 15, 563, [16] p. of plates :, ill. (some col.) ;, 27 cm.
    Edition Di 1 ban.
    Publisher Zhongguo nong ye chu ban she ; Xin hua shu dian Beijing fa xing suo fa xing
    Publishing place Beijing shi
    Document type Book
    ISBN 7109053199 ; 9787109053199
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: The technic parameters of slicing *Vitis* berries by freezing-microtome

    Wan, Y. / He Pu-chao

    Xibei Zhiwu Xuebao

    2001  Volume 21, Issue 2, Page(s) 382–386

    Abstract: After exploring the technic parameters of slicing *Vitis* berries by freezing-microtome, we founded an operation program: Harvesting the berry; putting the berry in the ultra-low temperature fridge to pre-freeze it at -28 °C to -30 °C for more than 8 ... ...

    Institution Northwest Sci-Tech Univ. of Agriculture and Forestry, College of Horticulture, Yangling, Shaanxi, 71200, People's Rep. of China
    Abstract After exploring the technic parameters of slicing *Vitis* berries by freezing-microtome, we founded an operation program: Harvesting the berry; putting the berry in the ultra-low temperature fridge to pre-freeze it at -28 °C to -30 °C for more than 8 hours; cutting the berry to make it have a smooth section for fixing; fixing the berry onto the freezing table of the microtome; freezing the berry quickly (at -35 °C ,10-20 seconds); sectioning the berry while the temperature kept at -20 °C (finishing it within 30 seconds); encapsulating the sections; observing the sections in the microscope. It was convenient if the section-thickness was 25-30 µm. Water-free glycerin as the section encapsulating agent was helpful for the observation of the berry pigment cells. [15638] (Auth. abstract)
    Keywords BERRY ; ANALYSIS ; TECHNIQUE ; HISTOLOGY ; CYTOLOGY
    Language Chamorro ; English
    Document type Article
    ISSN 1000-4025
    Database Viticulture and Oenology Abstracts

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