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  1. Article: TSG6 Plays a Role in Improving Orbital Inflammatory Infiltration and Extracellular Matrix Accumulation in TAO Model Mice.

    He, Xiuhui / Chen, Siya / Wang, Xiaohui / Kong, Min / Shi, Fangzheng / Qi, Xiaoxuan / Xu, Yuxin

    Journal of inflammation research

    2023  Volume 16, Page(s) 1937–1948

    Abstract: Introduction: TSG-6 plays a wide anti-inflammatory and therapeutic role in a variety of autoimmune diseases as the key mediator of mesenchymal stem cells (MSCs).: Purpose: We aimed to test whether TSG-6 could exert similar effects as MSCS in TAO via ... ...

    Abstract Introduction: TSG-6 plays a wide anti-inflammatory and therapeutic role in a variety of autoimmune diseases as the key mediator of mesenchymal stem cells (MSCs).
    Purpose: We aimed to test whether TSG-6 could exert similar effects as MSCS in TAO via establishing TAO animal model immunized by hTSHR-A subunit plasmid.
    Material and methods: We tested the expression level of TSG-6 on intraconal orbital fat from controls and patients with TAO. We established a stable thyroid-associated ophthalmopathy animal model by immunizing 6-week-old female Balb/c mice with recombination hTSHR-A subunit plasmid. After four immunizations, TSG-6 or dexamethasone was injected through the tail vein. The effects of the drugs on body weight, thyroid function, orbital inflammation, fibrosis and lipogenesis were observed.
    Results: The expression of TSG-6 in the orbital tissues of TAO patient is lower than that of normal people. In our animal model, mice showed weight loss, higher TT4 and TSHR antibody levels, and ocular symptoms such as inflammation and proptosis. TSG-6 can reduce ocular fibrosis and lipogenesis by inhibiting the infiltration of CD3+ T lymphocytes and macrophages in the mouse model of thyroid associated ophthalmopathy. Compared with dexamethasone, TSG-6 showed comparable anti-inflammatory effect, moreover, it has given a better performance in inhibiting adipogenesis.
    Conclusion: It was demonstrated that TSG-6 has a considerable positive impact on improving eye symptoms of TAO mice, which could be a novel candidate for the early treatment of TAO.
    Language English
    Publishing date 2023-05-04
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2494878-0
    ISSN 1178-7031
    ISSN 1178-7031
    DOI 10.2147/JIR.S409286
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Determination of Iodine in Geochemical Samples by ICP-MS with Sodium Carbonate-Zinc Oxide Semi-melting

    HE Xiuhui / TANG Shuaishuai / CHENG Jiang / SHI Youchang / LU Qianshu / WANG Yinjian / LAN Mingguo

    Yankuang ceshi, Vol 41, Iss 4, Pp 606-

    2022  Volume 613

    Abstract: BACKGROUND The determination of iodine in geochemical samples by inductively coupled plasma-mass spectrometry (ICP-MS) is treated mainly by closed sample melting, mixed acid solution, alkali fusion and semi-melting method. However, due to the complex ... ...

    Abstract BACKGROUND The determination of iodine in geochemical samples by inductively coupled plasma-mass spectrometry (ICP-MS) is treated mainly by closed sample melting, mixed acid solution, alkali fusion and semi-melting method. However, due to the complex existent morphology of iodine in soil and sediment samples, including periodate, iodate and iodide ions, and the first ionization energy of iodine being high as a halogen group element, there are problems such as incomplete dissolution, strong memory effect and poor precision during sample processing and measurement. OBJECTIVES To improve the determination of iodine in geochemical samples by ICP-MS. METHODS The samples were treated by sodium carbonate-zinc oxide semi-melting method, extracted with boiling water-ethanol, and separated by 732 cation exchange resin. Following this, iodine in the solution was determined by ICP-MS using an internal standard method. RESULTS The optimized detection limit of iodine was 0.045μg/g, the lower limit of detection was 0.15μg/g. The precision (RSD, n=12) and the accuracy (△logC) of the method were ≤5.93% and ≤0.01, respectively, which satisfied the analysis standards of geochemical survey sample. CONCLUSIONS This method meets the requirements of sample analysis for geochemical investigation, and can be used for the analysis of iodine in large quantities of soil and sediment samples.
    Keywords inductively coupled plasma-mass spectrometry ; geochemical sample ; na2co3-zno ; cation exchange resin ; iodine ; Geology ; QE1-996.5 ; Ecology ; QH540-549.5
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher Science Press, PR China
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: 35MHz quartz crystal microbalance and surface plasmon resonance studies on the binding of angiotensin converting enzyme with lisinopril

    Su, Zhaohong / Chen, Li / Liu, Ying / He, Xiuhui / Zhou, Yaping / Xie, Qingji / Yao, Shouzhuo

    Biosensors & bioelectronics. 2011 Mar. 15, v. 26, no. 7

    2011  

    Abstract: Angiotensin converting enzyme (ACE) plays a pivotal role in blood pressure regulation, and its interaction with an ACE inhibitor (ACEI) is an important research topic for treatment of hypertension. Herein, a low reagent consumption, multiparameter and ... ...

    Abstract Angiotensin converting enzyme (ACE) plays a pivotal role in blood pressure regulation, and its interaction with an ACE inhibitor (ACEI) is an important research topic for treatment of hypertension. Herein, a low reagent consumption, multiparameter and highly sensitive quartz crystal microbalance (QCM) at 35-MHz fundamental frequency was utilized to monitor in situ the binding process of solution lisinopril (LIS, a carboxylic third-generation ACEI) to ACE adsorbed at a 1-dodecanethiol (C12SH)-modified Au electrode. From the QCM data, the binding molar ratio (r) of LIS to adsorbed ACE was estimated to be 2.3:1, and the binding and dissociation rate constants (k₁ and k₋₁) and the binding equilibrium constant (Kₐ) were estimated to be k₁=4.1×10⁶Lmol⁻¹s⁻¹, k₋₁=7.3×10⁻³s⁻¹ and Kₐ=5.62×10⁸Lmol⁻¹, respectively. Comparable qualitative and quantitative results were also obtained from separate experiments of cyclic voltammetry, electrochemical impedance spectroscopy and surface plasmon resonance measurements.
    Keywords angiotensin-converting enzyme inhibitors ; biosensors ; blood pressure ; dielectric spectroscopy ; dissociation ; electrodes ; gold ; hypertension ; peptidyl-dipeptidase A ; quartz ; surface plasmon resonance
    Language English
    Dates of publication 2011-0315
    Size p. 3240-3245.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2010.12.033
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
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  4. Article ; Online: 35 MHz quartz crystal microbalance and surface plasmon resonance studies on the binding of angiotensin converting enzyme with lisinopril.

    Su, Zhaohong / Chen, Li / Liu, Ying / He, Xiuhui / Zhou, Yaping / Xie, Qingji / Yao, Shouzhuo

    Biosensors & bioelectronics

    2011  Volume 26, Issue 7, Page(s) 3240–3245

    Abstract: Angiotensin converting enzyme (ACE) plays a pivotal role in blood pressure regulation, and its interaction with an ACE inhibitor (ACEI) is an important research topic for treatment of hypertension. Herein, a low reagent consumption, multiparameter and ... ...

    Abstract Angiotensin converting enzyme (ACE) plays a pivotal role in blood pressure regulation, and its interaction with an ACE inhibitor (ACEI) is an important research topic for treatment of hypertension. Herein, a low reagent consumption, multiparameter and highly sensitive quartz crystal microbalance (QCM) at 35-MHz fundamental frequency was utilized to monitor in situ the binding process of solution lisinopril (LIS, a carboxylic third-generation ACEI) to ACE adsorbed at a 1-dodecanethiol (C12SH)-modified Au electrode. From the QCM data, the binding molar ratio (r) of LIS to adsorbed ACE was estimated to be 2.3:1, and the binding and dissociation rate constants (k(1) and k(-1)) and the binding equilibrium constant (K(a)) were estimated to be k(1)=4.1×10(6) L mol(-1) s(-1), k(-1)=7.3×10(-3) s(-1) and K(a)=5.62×10(8) L mol(-1), respectively. Comparable qualitative and quantitative results were also obtained from separate experiments of cyclic voltammetry, electrochemical impedance spectroscopy and surface plasmon resonance measurements.
    MeSH term(s) Angiotensin-Converting Enzyme Inhibitors/pharmacology ; Antihypertensive Agents/pharmacology ; Humans ; Lisinopril/pharmacology ; Peptidyl-Dipeptidase A/metabolism ; Protein Binding ; Quartz Crystal Microbalance Techniques/methods ; Surface Plasmon Resonance/methods
    Chemical Substances Angiotensin-Converting Enzyme Inhibitors ; Antihypertensive Agents ; Lisinopril (E7199S1YWR) ; Peptidyl-Dipeptidase A (EC 3.4.15.1)
    Language English
    Publishing date 2011-03-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2010.12.033
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2275: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Amperometric enzyme electrodes of glucose and lactate based on poly(diallyldimethylammonium)-alginate-metal ion-enzyme biocomposites.

    Qin, Cong / Chen, Chao / Xie, Qingji / Wang, Lihua / He, Xiuhui / Huang, Yi / Zhou, Yaping / Xie, Fangyun / Yang, Dawei / Yao, Shouzhuo

    Analytica chimica acta

    2012  Volume 720, Page(s) 49–56

    Abstract: Sodium alginate (AlgNa) and poly(diallyldimethylammonium chloride) (PDDA) were mixed to obtain an interpenetrating polymer composite via electrostatic interaction and then cast on an Au electrode surface, followed by incorporation of metal ions (e.g. Fe( ... ...

    Abstract Sodium alginate (AlgNa) and poly(diallyldimethylammonium chloride) (PDDA) were mixed to obtain an interpenetrating polymer composite via electrostatic interaction and then cast on an Au electrode surface, followed by incorporation of metal ions (e.g. Fe(3+) or Ca(2+), to form AlgFe or AlgCa hydrogel) and glucose oxidase (GOx) (or lactate oxidase (LOx)), to prepare amperometric enzyme electrodes. The interactions of PDDA, Alg, and Fe(3+) are studied by visual inspection as well as microscopic and electrochemical methods. Under optimized conditions, the PDDA-AlgFe-enzyme/Au and PDDA-AlgCa-enzyme/Au electrodes can give good analytical performance (e.g. nM-scale limit of detection of glucose or lactate, and sensitivities > 50 μA cm(-2) mM(-1)) in the first-generation biosensing mode, which are better than the reported analogs using typical polysaccharide biopolymers as enzyme-immobilization matrices. The enzyme electrodes also worked well in the second-generation biosensing mode in the coexistence of p-benzoquione or ferrocene monocarboxylic acid artificial mediator. Biofuel cells (BFCs) with the enzyme electrodes as the bioanodes and glucose (or lactate) as the biofuel were also fabricated with satisfactory results. The proposed protocols for preparation of high performance Alg-based biocomposites may find wide applications in bioanalysis.
    MeSH term(s) Alginates/chemistry ; Bioelectric Energy Sources ; Biosensing Techniques ; Electrochemical Techniques ; Enzymes, Immobilized/metabolism ; Ferric Compounds/chemistry ; Glucose/analysis ; Glucose Oxidase/metabolism ; Glucuronic Acid/chemistry ; Hexuronic Acids/chemistry ; Ions/chemistry ; Lactic Acid/analysis ; Metals/chemistry ; Mixed Function Oxygenases/metabolism ; Polyethylenes/chemistry ; Quaternary Ammonium Compounds/chemistry
    Chemical Substances Alginates ; Enzymes, Immobilized ; Ferric Compounds ; Hexuronic Acids ; Ions ; Metals ; Polyethylenes ; Quaternary Ammonium Compounds ; poly-N,N-dimethyl-N,N-diallylammonium chloride (26062-79-3) ; Lactic Acid (33X04XA5AT) ; Glucuronic Acid (8A5D83Q4RW) ; alginic acid (8C3Z4148WZ) ; Mixed Function Oxygenases (EC 1.-) ; Glucose Oxidase (EC 1.1.3.4) ; lactate 2-monooxygenase (EC 1.13.12.4) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2012-03-30
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2012.01.033
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Amperometric enzyme electrodes of glucose and lactate based on poly(diallyldimethylammonium)-alginate-metal ion-enzyme biocomposites

    Qin, Cong / Chen, Chao / Xie, Qingji / Wang, Lihua / He, Xiuhui / Huang, Yi / Zhou, Yaping / Xie, Fangyun / Yang, Dawei / Yao, Shouzhuo

    Analytica chimica acta. 2012 Mar. 30, v. 720

    2012  

    Abstract: Sodium alginate (AlgNa) and poly(diallyldimethylammonium chloride) (PDDA) were mixed to obtain an interpenetrating polymer composite via electrostatic interaction and then cast on an Au electrode surface, followed by incorporation of metal ions (e.g. Fe³⁺ ...

    Abstract Sodium alginate (AlgNa) and poly(diallyldimethylammonium chloride) (PDDA) were mixed to obtain an interpenetrating polymer composite via electrostatic interaction and then cast on an Au electrode surface, followed by incorporation of metal ions (e.g. Fe³⁺ or Ca²⁺, to form AlgFe or AlgCa hydrogel) and glucose oxidase (GOx) (or lactate oxidase (LOx)), to prepare amperometric enzyme electrodes. The interactions of PDDA, Alg, and Fe³⁺ are studied by visual inspection as well as microscopic and electrochemical methods. Under optimized conditions, the PDDA-AlgFe-enzyme/Au and PDDA-AlgCa-enzyme/Au electrodes can give good analytical performance (e.g. nM-scale limit of detection of glucose or lactate, and sensitivities>50μAcm⁻²mM⁻¹) in the first-generation biosensing mode, which are better than the reported analogs using typical polysaccharide biopolymers as enzyme-immobilization matrices. The enzyme electrodes also worked well in the second-generation biosensing mode in the coexistence of p-benzoquione or ferrocene monocarboxylic acid artificial mediator. Biofuel cells (BFCs) with the enzyme electrodes as the bioanodes and glucose (or lactate) as the biofuel were also fabricated with satisfactory results. The proposed protocols for preparation of high performance Alg-based biocomposites may find wide applications in bioanalysis.
    Keywords biocomposites ; biofuels ; biopolymers ; calcium ; composite polymers ; detection limit ; electrochemistry ; electrodes ; electrostatic interactions ; glucose ; glucose oxidase ; gold ; hydrocolloids ; iron ; lactate 2-monooxygenase ; metal ions ; microbial fuel cells ; sodium alginate
    Language English
    Dates of publication 2012-0330
    Size p. 49-56.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2012.01.033
    Database NAL-Catalogue (AGRICOLA)

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