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Article ; Online: Multigram-scale enzymatic kinetic resolution of trans-2-azidocyclohexyl acetate and chiral reversed-phase HPLC analysis of trans-2-azidocyclohexanol.

Hebda, Paulina / Wiśniowska, Lilianna / Szafrański, Przemysław W / Cegła, Marek

2021 Volume 34, Issue 2, Page(s) 428–437

Abstract: Lipase-catalyzed hydrolytic kinetic resolution is a method of obtaining optically pure chiral alcohols and amines, which requires additional tools for determining enantiomerical purity. Herein, we present a study on multigram-scale hydrolytic kinetic ... ...

Abstract	Lipase-catalyzed hydrolytic kinetic resolution is a method of obtaining optically pure chiral alcohols and amines, which requires additional tools for determining enantiomerical purity. Herein, we present a study on multigram-scale hydrolytic kinetic resolution of trans-2-azidocyclohexyl acetate using Pseudomonas cepacia lipase immobilized on Immobead support. We investigated several parameters of the preparative-scale process: temperature, organic co-solvent, and the influence of calcium ions. Moreover, we have developed an efficient fluorenylmethyloxycarbonyl chloride (Fmoc-Cl) derivatization protocol for 2-azidocyclohexanol, which enabled chiral reversed-phase high-performance liquid chromatography (RP-HPLC) determination of enantiomeric excess.
MeSH term(s)	Acetates ; Alcohols ; Chromatography, High Pressure Liquid/methods ; Lipase/chemistry ; Stereoisomerism
Chemical Substances	Acetates ; Alcohols ; Lipase (EC 3.1.1.3)
Language	English
Publishing date	2021-11-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1011639-4
ISSN	1520-636X ; 0899-0042
ISSN (online)	1520-636X
ISSN	0899-0042
DOI	10.1002/chir.23397
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Zs.A 2594: Show issues

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Article ; Online: Skin wound healing and scarring: fetal wounds and regenerative restitution.

Yates, Cecelia C / Hebda, Patricia / Wells, Alan

Birth defects research. Part C, Embryo today : reviews

2013 Volume 96, Issue 4, Page(s) 325–333

Abstract: The adverse physiological and psychological effects of scars formation after healing of wounds are broad and a major medical problem for patients. In utero, fetal wounds heal in a regenerative manner, though the mechanisms are unknown. Differences in ... ...

Abstract	The adverse physiological and psychological effects of scars formation after healing of wounds are broad and a major medical problem for patients. In utero, fetal wounds heal in a regenerative manner, though the mechanisms are unknown. Differences in fetal scarless regeneration and adult repair can provide key insight into reduction of scarring therapy. Understanding the cellular and extracellular matrix alterations in excessive adult scarring in comparison to fetal scarless healing may have important implications. Herein, we propose that matrix can be controlled via cellular therapy to resemble a fetal-like matrix that will result in reduced scarring.
MeSH term(s)	Apoptosis/physiology ; Cell- and Tissue-Based Therapy ; Cicatrix/prevention & control ; Cicatrix/therapy ; Extracellular Matrix/physiology ; Fetus/pathology ; Fibroblasts/metabolism ; Humans ; Phenotype ; Receptors, CXCR3/metabolism ; Regeneration/physiology ; Signal Transduction ; Skin/injuries ; Wound Healing/genetics ; Wound Healing/physiology
Chemical Substances	CXCR3 protein, human ; Receptors, CXCR3
Language	English
Publishing date	2013-11-06
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2104792-3
ISSN	1542-9768 ; 1542-0752 ; 1542-9733 ; 1542-975X
ISSN (online)	1542-9768
ISSN	1542-0752 ; 1542-9733 ; 1542-975X
DOI	10.1002/bdrc.21024
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Zs.A 749: Show issues

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Article: Stimulatory effects of transforming growth factor-beta and epidermal growth factor on epidermal cell outgrowth from porcine skin explant cultures.

Hebda, P A

The Journal of investigative dermatology

1988 Volume 91, Issue 5, Page(s) 440–445

Abstract: Transforming growth factor-beta (TGF-beta) is known to stimulate dermal wound healing events (fibroplasia and fibrosis). In this study, the effect of TGF-beta on epidermal wound healing (re-epithelialization) was examined. Epidermal cell outgrowth from ... ...

Abstract	Transforming growth factor-beta (TGF-beta) is known to stimulate dermal wound healing events (fibroplasia and fibrosis). In this study, the effect of TGF-beta on epidermal wound healing (re-epithelialization) was examined. Epidermal cell outgrowth from partial-thickness porcine skin explants was used as an in vitro model for epithelialization. All cultures were grown in medium with 1% fetal bovine serum, which was sufficient for explant viability but low enough to permit measurement of modulation by added factors. Because TGF-beta is known to act in concert with other growth factors, it was evaluated alone and in the presence of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF). The results indicate that TGF-beta produced earlier initiation of outgrowth, by 1-2 d compared with control cultures, and increased the rate of outgrowth during the migratory phase of culture (Days 1-3). Compared to controls, EGF alone produced a greater percentage of growing explants and an increased rate of outgrowth during the mitotic phase (Days 4-7). TGF-beta (1 or 10 ng/ml) and EGF (5 ng/ml) had an additive rather than a synergistic effect on outgrowth. PDGF-treated explants did not show enhanced growth when PDGF (2.5 units/ml) was added alone or together with TGF-beta and EGF. The ability of TGF-beta to produce earlier initiation of outgrowth was not due to an effect on mitosis, because TGF-beta did not increase the incorporation of [3H]thymidine into keratinocytes in the growing epidermal sheets. Rather, it is likely that TGF-beta facilitated keratinocyte migration, possibly by unmasking a receptor on the epidermal cell surface. These results suggest that TGF-beta may play a role in early epidermal wound healing.
MeSH term(s)	Animals ; Autoradiography ; Cell Division/drug effects ; Cell Movement/drug effects ; Culture Techniques ; Drug Combinations ; Epidermal Cells ; Epidermal Growth Factor/pharmacology ; Epidermis/drug effects ; Mitosis/drug effects ; Platelet-Derived Growth Factor/pharmacology ; Skin/cytology ; Swine ; Transforming Growth Factors/pharmacology
Chemical Substances	Drug Combinations ; Platelet-Derived Growth Factor ; Epidermal Growth Factor (62229-50-9) ; Transforming Growth Factors (76057-06-2)
Language	English
Publishing date	1988-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	80136-7
ISSN	1523-1747 ; 0022-202X
ISSN (online)	1523-1747
ISSN	0022-202X
DOI	10.1111/1523-1747.ep12476480
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Article: Transplanted fetal fibroblasts: survival and distribution over time in normal adult dermis compared with autogenic, allogenic, and xenogenic adult fibroblasts.

Hebda, P A / Dohar, J E

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery

1999 Volume 121, Issue 3, Page(s) 245–251

Abstract: Cell therapy is a widely applicable therapeutic approach using cells and cell elements, frequently from fetal or young animals, for their beneficial effects. This study evaluated the host response to and tolerance of transplanted fetal skin fibroblasts. ... ...

Abstract	Cell therapy is a widely applicable therapeutic approach using cells and cell elements, frequently from fetal or young animals, for their beneficial effects. This study evaluated the host response to and tolerance of transplanted fetal skin fibroblasts. Cultured fibroblasts from adult rabbit skin (autogenic and allogenic), 21-day fetal rabbit skin (allogenic), and adult pig skin (xenogenic) were labeled with a fluorescent vital dye CM-DiI, injected intradermally into the dorsal skin of adult rabbits at multiple sites and then biopsied over an 8-week period. Each cell type showed a biphasic distribution curve with an early phase (0 to 28 days) and a late phase (28 to 56 days). In the early phase, cells showed a rise and fall in total cell density (reflecting an increase and then a decrease in total cell number), followed by a slow decrease in cell density with cells still detectable at 56 days. Fetal cells showed the highest survival at the end of the study. None of the groups showed clinical or histologic signs of acute inflammation or rejection. This study demonstrated that (1) transplanted fibroblasts are well tolerated by an immunologically competent host, (2) CM-DiI-labeled cells are detectable in vivo for at least 8 weeks, and (3) fetal fibroblasts have a distribution and survival profile that is distinct from that of adult fibroblasts.
MeSH term(s)	Age Factors ; Animals ; Cell Division ; Cell Survival ; Cells, Cultured ; Fetus/cytology ; Fibroblasts/transplantation ; Rabbits ; Skin/cytology ; Swine ; Transplantation, Autologous ; Transplantation, Heterologous ; Transplantation, Homologous
Language	English
Publishing date	1999-09
Publishing country	England
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	392085-9
ISSN	1097-6817 ; 0194-5998 ; 0161-6439
ISSN (online)	1097-6817
ISSN	0194-5998 ; 0161-6439
DOI	10.1016/S0194-5998(99)70179-8
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Article: Expression of interleukin 2 receptors on human carcinoma cell lines and tumor growth inhibition by interleukin 2.

Yasumura, S / Lin, W C / Weidmann, E / Hebda, P / Whiteside, T L

International journal of cancer

1994 Volume 59, Issue 2, Page(s) 225–234

Abstract: We have previously shown that human squamous cell carcinomas (SCC) express the interleukin 2 receptor (IL2R)-alpha and -beta chains, and that the ligand, IL2, directly inhibits growth of the tumor in vitro and in vivo in the tumor xenograft-nude mice ... ...

Abstract	We have previously shown that human squamous cell carcinomas (SCC) express the interleukin 2 receptor (IL2R)-alpha and -beta chains, and that the ligand, IL2, directly inhibits growth of the tumor in vitro and in vivo in the tumor xenograft-nude mice model. We now show that the alpha and beta chains of IL2R are expressed on a variety of human carcinoma cell lines and on normal human keratinocytes in early-stage cultures. While all carcinoma cells in a population expressed IL2R-alpha and -beta proteins, in keratinocytes obtained from different normal donors, variable proportions of cells were positive, as measured by flow cytometry. The carcinoma lines and 2/5 keratinocyte lines studied were also found to contain transcripts for the IL2R-gamma chain detectable by combined reverse transcription-PCR (RT-PCR) and hybridization with the specific cDNA probe. Incubation of the gastric (HR) or renal cell carcinoma (RCC) cell lines, but not of other IL2R+ carcinoma cell lines or normal keratinocytes, in the presence of IL2 resulted in dose-dependent inhibition of tumor cell growth. Monoclonal antibodies (MAbs) specific for IL2R-beta chain completely reversed this growth inhibitory effect of IL2. The ligand, IL2, also down-regulated surface expression of its own receptor and of intercellular adhesion molecule-I (ICAM-I) or class I major histocompatibility complex (MHC) antigens on IL2R+ tumor cells. All carcinoma cells studied incubated in the presence of IL2 exhibited significantly increased sensitivity to growth-inhibitory effects of other cytokines such as interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha or transforming growth factor (TGF)-beta. IL2 inhibited growth of the HR cells by arresting a significant proportion of tumor cells in the G0/G1 phase of the cell cycle. Thus, IL2 can have direct effects on IL2R+ carcinoma cells, leading to changes in growth or to increases in sensitivity of tumor cells to cytostatic activities of other cytokines.
MeSH term(s)	Antigens, Neoplasm/physiology ; Base Sequence ; Carcinoma, Renal Cell/drug therapy ; Carcinoma, Renal Cell/pathology ; Carcinoma, Renal Cell/ultrastructure ; Carcinoma, Squamous Cell/drug therapy ; Carcinoma, Squamous Cell/pathology ; Carcinoma, Squamous Cell/ultrastructure ; Cell Cycle/drug effects ; Cell Division/drug effects ; Cytokines/pharmacology ; Drug Screening Assays, Antitumor ; Head and Neck Neoplasms/drug therapy ; Head and Neck Neoplasms/pathology ; Head and Neck Neoplasms/ultrastructure ; Humans ; In Situ Hybridization ; Interleukin-2/pharmacology ; Keratinocytes/cytology ; Keratinocytes/drug effects ; Kidney Neoplasms/drug therapy ; Kidney Neoplasms/pathology ; Kidney Neoplasms/ultrastructure ; Molecular Sequence Data ; Neoplasms/drug therapy ; Neoplasms/pathology ; Neoplasms/ultrastructure ; Polymerase Chain Reaction ; RNA, Messenger/analysis ; RNA-Directed DNA Polymerase ; Receptors, Interleukin-2/genetics ; Receptors, Interleukin-2/physiology ; Signal Transduction/drug effects ; Signal Transduction/physiology ; Stomach Neoplasms/drug therapy ; Stomach Neoplasms/pathology ; Stomach Neoplasms/ultrastructure ; Transcription, Genetic ; Tumor Cells, Cultured/drug effects
Chemical Substances	Antigens, Neoplasm ; Cytokines ; Interleukin-2 ; RNA, Messenger ; Receptors, Interleukin-2 ; RNA-Directed DNA Polymerase (EC 2.7.7.49)
Language	English
Publishing date	1994-10-15
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218257-9
ISSN	1097-0215 ; 0020-7136
ISSN (online)	1097-0215
ISSN	0020-7136
DOI	10.1002/ijc.2910590215
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Zs.A 478: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 576: Show issues

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Article: Effects of sex hormones on protein and collagen content of the temporomandibular joint disc of the rat.

Abubaker, A O / Hebda, P C / Gunsolley, J N

Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons

1996 Volume 54, Issue 6, Page(s) 721–7; discussion 727–8

Abstract: Purpose: The effect of sex hormones on the protein and collagen content of the temporomandibular joint (TMJ) disc of adult male and female rats.: Materials and methods: One hundred forty-four Wistar rats were assigned to 14 groups of 12 each. Two ... ...

Abstract	Purpose: The effect of sex hormones on the protein and collagen content of the temporomandibular joint (TMJ) disc of adult male and female rats. Materials and methods: One hundred forty-four Wistar rats were assigned to 14 groups of 12 each. Two groups, one female and one male, served as a control and received no treatment, and two other groups (one female and one male) received a sham gonadectomy and placebo hormone. The remaining 10 groups (five males and five females) received either orchiectomy or ovariectomy, followed by administration of estrogen, progesterone, combined estrogen and progesterone, or testosterone. The total protein and collagen content of the TMJ disc were determined using the calorimetric hydroxyproline method. Results: The collagen content of TMJ discs of control males was statistically greater than the collagen content of the control female rats. This difference disappeared after ovariectomy of females and orchiectomy of males. Also, there was a general trend for a decrease in collagen and protein content to be produced by estrogen, progesterone, and by estrogen combined with progesterone in castrated male and female rats, and by orchiectomy of male rats. There was also a trend toward an increase in collagen and protein content after ovariectomy in female rats and administration of testosterone to castrated male and female rats. However, the only statistically significant effect of the drugs tested was that of estrogen combined with progesterone in ovariectomized female rats (a lowering effect on the total protein) and of estrogen alone in orchiectomized male rats (a lowering effect on the collagen content). Conclusion: Steroid sex hormones have an effect on the collagen and protein content of the TMJ disc of the rat as indicated by the difference in the values between control males and females and by the disappearance of this difference on castration of both male and female animals. This was also manifested by the significant effect of estradiol on collagen content of castrated males, by the effect of estrogen combined with progesterone on the protein content of castrated females.
MeSH term(s)	Animals ; Calorimetry ; Cartilage, Articular/chemistry ; Cartilage, Articular/drug effects ; Collagen/analysis ; Collagen/drug effects ; Drug Combinations ; Estrogens/administration & dosage ; Estrogens/pharmacology ; Female ; Hydroxyproline/analysis ; Hydroxyproline/drug effects ; Indicators and Reagents ; Male ; Ninhydrin ; Orchiectomy ; Ovariectomy ; Placebos ; Progesterone/administration & dosage ; Progesterone/pharmacology ; Proteins/analysis ; Proteins/drug effects ; Rats ; Rats, Wistar ; Sex Factors ; Temporomandibular Joint/chemistry ; Temporomandibular Joint/drug effects ; Testosterone/pharmacology
Chemical Substances	Drug Combinations ; Estrogens ; Indicators and Reagents ; Placebos ; Proteins ; Testosterone (3XMK78S47O) ; Progesterone (4G7DS2Q64Y) ; Collagen (9007-34-5) ; Ninhydrin (HCL6S9K23A) ; Hydroxyproline (RMB44WO89X)
Language	English
Publishing date	1996-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	392404-x
ISSN	1531-5053 ; 0278-2391
ISSN (online)	1531-5053
ISSN	0278-2391
DOI	10.1016/s0278-2391(96)90690-4
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Ul III Zs.113: Show issues

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Article: Heterogeneous synthetic phenotype of cloned scleroderma fibroblasts may be due to aberrant regulation in the synthesis of connective tissues.

Whiteside, T L / Ferrarini, M / Hebda, P / Buckingham, R B

Arthritis and rheumatism

1988 Volume 31, Issue 10, Page(s) 1221–1229

Abstract: Clones of dermal fibroblasts from the skin of 4 normal subjects and 5 patients with progressive systemic sclerosis (PSS; scleroderma) were established, and their synthetic and proliferative characteristics were compared. A limiting-dilution assay was ... ...

Abstract	Clones of dermal fibroblasts from the skin of 4 normal subjects and 5 patients with progressive systemic sclerosis (PSS; scleroderma) were established, and their synthetic and proliferative characteristics were compared. A limiting-dilution assay was used to determine frequencies of cloning in the microcultures of dermal fibroblasts plated. The clones derived from single cells were expanded in vitro and examined (in passages C-H) for growth and synthesis of glycosaminoglycan (GAG) and collagenase-sensitive protein (CSP). The clonogenicity of PSS fibroblasts was not significantly different from that of normal fibroblasts. Normal fibroblast clones were characterized by low levels of GAG and CSP synthesis, and there was a correlation between the GAG and CSP phenotypes. In contrast, clones of PSS fibroblasts were often, but not always, high producers of GAG and CSP, but there was no correlation between the levels of GAG and CSP synthesis. It appears that scleroderma skin is composed of fibroblast clones that are unable to regulate the synthesis of connective tissue components and often synthesize large amounts of connective tissue macromolecules.
MeSH term(s)	Adult ; Cell Division ; Clone Cells ; Connective Tissue/physiopathology ; Fibroblasts/metabolism ; Fibroblasts/pathology ; Fibroblasts/physiology ; Glycosaminoglycans/metabolism ; Humans ; Microbial Collagenase/metabolism ; Phenotype ; Proteins/metabolism ; Reference Values ; Scleroderma, Systemic/genetics ; Scleroderma, Systemic/metabolism ; Scleroderma, Systemic/pathology ; Skin/metabolism ; Skin/pathology ; Skin Physiological Phenomena
Chemical Substances	Glycosaminoglycans ; Proteins ; Microbial Collagenase (EC 3.4.24.3)
Language	English
Publishing date	1988-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	127294-9
ISSN	1529-0131 ; 0004-3591 ; 2326-5191
ISSN (online)	1529-0131
ISSN	0004-3591 ; 2326-5191
DOI	10.1002/art.1780311002
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Zs.A 24: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 523: Show issues

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Article: Fetal airway wound repair: a new frontier.

Dohar, J E / Klein, E C / Betsch, J L / Hebda, P A

Archives of otolaryngology--head & neck surgery

1998 Volume 124, Issue 1, Page(s) 25–29

Abstract: Purpose: Fetal dermal repair is regenerative and scarless until middle to late gestation, when there is a transition to fibrotic repair. Fetal skeletal muscle and tendon undergo repair with fibrosis similar to the process in adults. This study addresses ...

Abstract	Purpose: Fetal dermal repair is regenerative and scarless until middle to late gestation, when there is a transition to fibrotic repair. Fetal skeletal muscle and tendon undergo repair with fibrosis similar to the process in adults. This study addresses whether fetal mucosal healing is regenerative and scarless. Methods: Anesthetized pregnant rabbits underwent laparotomy and controlled hysterotomy at 21 to 23 days' gestation (term is 31 days). A midline thyrotomy was made, followed by cricoidotomy and circumferential cauterization of the subglottic mucosa. A similar insult was applied to weanlings. The data were collected in 2 groups. One group was followed to term and killed at 4 weeks. A second group was killed after 6 days (30 days' gestation). The weanlings were killed at similar points. The larynges were harvested and processed for histological and morphometric analysis. Results: Three litters were followed to term. Of these, 1 was not recovered; in the other two, 7 of 8 manipulated fetuses were found and 3 of 8 were viable. The fourth litter was harvested after 6 days; all 4 injured fetuses were recovered and viable. All animals in the fetal injury groups healed with complete regeneration of the airway mucosa. In contrast, weanlings injured post partum had mucosal inflammation, necrosis, and ulceration; squamous metaplasia and basal cell hyperplasia were also found. There were fibrosis, granulation tissue, and inflammation in the lamina propria; chondritis, cartilaginous necrosis, chondrolysis, and perichondritis were also found. Conclusions: Fetal airway mucosal healing is regenerative and, thus, scarless. This study provides further support for the thesis that skin and mucosa respond to injury similarly in both the developmental and postpartum stages, and that subglottic stenosis is reasonably thought of as the "hyperplastic scar" of the airway. These results have potential therapeutic applications for mucosal wound management.
MeSH term(s)	Animals ; Female ; Fetus/surgery ; Glottis/embryology ; Glottis/ultrastructure ; Larynx/embryology ; Larynx/injuries ; Larynx/surgery ; Mucous Membrane/embryology ; Mucous Membrane/ultrastructure ; Pregnancy ; Rabbits ; Wound Healing
Language	English
Publishing date	1998-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	632721-7
ISSN	1538-361X ; 0886-4470 ; 2168-6181
ISSN (online)	1538-361X
ISSN	0886-4470 ; 2168-6181
DOI	10.1001/archotol.124.1.25
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Article: Wound management of the airway mucosa: comparison with skin in a rabbit model.

Goldstein, N A / Hebda, P A / Klein, E C / Dohar, J E

International journal of pediatric otorhinolaryngology

1998 Volume 45, Issue 3, Page(s) 223–235

Abstract: In comparison to the extensive study of skin wound healing, there have been few reports investigating mucosal wound healing. Our primary objective was to compare the natural progression of wound healing in airway mucosa to skin in a rabbit model. Split- ... ...

Abstract	In comparison to the extensive study of skin wound healing, there have been few reports investigating mucosal wound healing. Our primary objective was to compare the natural progression of wound healing in airway mucosa to skin in a rabbit model. Split-thickness skin wounds and subglottic mucosal wounds created by drill injury were compared on days 0, 1, 3, 5, 7, 14 and 21 after injury. Histologic examination was performed by a veterinary pathologist blinded to sample identity. Subglottic wounds showed a 'fibrinous clot' overlying the epithelium, analogous to the fibrin crust in skin wounds. Re-epithelialization started on day 5 in the subglottic epithelium and was complete by day 14; fibroplasia and fibrosis in the lamina propria were present on days 7-21. This wound healing profile paralleled the skin epidermis and dermis, respectively. The epithelial changes, however, were temporally extended in the airway. Our secondary objective was to determine the effects of treating airway mucosa with a bioresorbable membrane, modified sodium hyaluronate and carboxymethylcellulose (modified HA/CMC), placed over the subglottic wounds of four rabbits after drill injury. Subglottic wounds treated with modified HA/CMC showed a more mature epithelium and less fibrosis on day 21. In this pilot study, the application of a bioresorbable membrane improved mucosal wound healing at both the epithelial and lamina propria levels. Clearly, a larger study must be performed to confirm this interesting observation.
MeSH term(s)	Absorption ; Animals ; Carboxymethylcellulose Sodium ; Hyaluronic Acid ; Larynx/injuries ; Larynx/pathology ; Membranes, Artificial ; Mucous Membrane/injuries ; Mucous Membrane/pathology ; Rabbits ; Skin/injuries ; Skin/pathology ; Wound Healing
Chemical Substances	Membranes, Artificial ; Hyaluronic Acid (9004-61-9) ; Carboxymethylcellulose Sodium (K679OBS311)
Language	English
Publishing date	1998-10-15
Publishing country	Ireland
Document type	Comparative Study ; Journal Article
ZDB-ID	754501-0
ISSN	1872-8464 ; 0165-5876
ISSN (online)	1872-8464
ISSN	0165-5876
DOI	10.1016/s0165-5876(98)00117-7
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Zs.A 1507: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 134: Show issues

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Article: Acquired subglottic stenosis--depth and not extent of the insult is key.

Dohar, J E / Klein, E C / Betsch, J L / Hebda, P A

International journal of pediatric otorhinolaryngology

1998 Volume 46, Issue 3, Page(s) 159–170

Abstract: In contrast to skin, mucosal wound healing has not been extensively studied. Subglottic stenosis (SGS) is an excellent model for such investigation. The main objective of this pilot study was to develop a chronic model of SGS in a small animal (i.e. ... ...

Abstract	In contrast to skin, mucosal wound healing has not been extensively studied. Subglottic stenosis (SGS) is an excellent model for such investigation. The main objective of this pilot study was to develop a chronic model of SGS in a small animal (i.e. rabbit). In so doing, a serendipitous observation was made that the development of SGS is directly related to depth of the injury and is independent of circumferential extent. Animals with deep injury (i.e. deep to the lamina propria, reaching the perichondrium), independent of age and circumferential extent, experienced respiratory obstruction resulting from edema and granulation tissue formation and died or had to be sacrificed in the acute period. This was in contrast to no risk of mortality in the more superficially injured group. Histology was used to characterize this model of SGS. In the mucosal epithelium, or mucosa, changes of inflammation, squamous metaplasia, basal cell hyperplasia, necrosis and ulceration were only seen acutely and total regeneration of the epithelium was achieved by the end of the study period. In contrast, changes within the lamina propria, including chronic inflammatory cellular infiltrates and fibroplasia, were lasting and resulted in fibrotic repair, not regeneration. These findings are quite similar to the healing events in skin and suggest that SGS is the mucosal equivalent of a 'keloid' or, perhaps more appropriately, a 'hypertrophic scar.' Likewise, cartilage degeneration and deformation were persistent markers of the chronic phase of healing. Like the lamina propria, the response to injury was reparative. Therefore, injury to the connective tissue is a critical component of development of SGS.
MeSH term(s)	Animals ; Connective Tissue/injuries ; Connective Tissue/physiology ; Glottis/injuries ; Laryngostenosis/pathology ; Laryngostenosis/physiopathology ; Mucous Membrane/injuries ; Mucous Membrane/physiology ; Rabbits ; Wound Healing/physiology
Language	English
Publishing date	1998-12-15
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	754501-0
ISSN	1872-8464 ; 0165-5876
ISSN (online)	1872-8464
ISSN	0165-5876
DOI	10.1016/s0165-5876(98)00163-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

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