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  1. Article ; Online: Comparison of image quality of corneal and retinal optical coherence tomography using sedation and general anesthesia protocols with or without retrobulbar anesthesia in horses.

    Hefner, Ethan M / Lin, Hui-Chu / Cole, Robert C / Moore, Phillip A / McMullen, Richard J

    American journal of veterinary research

    2021  Volume 83, Issue 1, Page(s) 72–79

    Abstract: Objective: To compare image quality and acquisition time of corneal and retinal spectral domain optical coherence tomography (SD-OCT) under 3 different sedation-anesthesia conditions in horses.: Animals: 6 middle-aged geldings free of ocular disease.! ...

    Abstract Objective: To compare image quality and acquisition time of corneal and retinal spectral domain optical coherence tomography (SD-OCT) under 3 different sedation-anesthesia conditions in horses.
    Animals: 6 middle-aged geldings free of ocular disease.
    Procedures: 1 randomly selected eye of each horse was evaluated via SD-OCT under the following 3 conditions: standing sedation without retrobulbar anesthetic block (RB), standing sedation with RB, and general anesthesia with RB. Five regions of interest were evaluated in the cornea (axial and 12, 3, 6, and 9 o'clock positions) and fundus (optic nerve head). Three diagnostic scans of predetermined quality were obtained per anatomical region. Image acquisition times and total scans per site were recorded. Corneal and retinal SD-OCT image quality was graded on a subjective scale from 0 (nondiagnostic) to 4 (excellent).
    Results: Mean values for the standing sedation without RB, standing sedation with RB, and general anesthesia conditions were 24, 23, and 17, respectively, for total cornea scan attempts; 23, 19, and 19 for total retina-scan attempts; 14.6, 13.2, and 9.2 minutes for total cornea scan time; 19.1, 9.2, and 13.0 for total retina scan time; 2.0, 2.3, and 2.5 for cornea grade; and 2.7, 2.9, and 2.5 for retina grade.
    Conclusions and clinical relevance: The RB facilitated globe akinesia and improved the percentage of scans in frame and region of interest accuracy for retinal imaging via OCT in horses. Retrobulbar blocks improved clinical image acquisition while minimizing motion artifact.
    MeSH term(s) Anesthesia, General/veterinary ; Animals ; Cornea/diagnostic imaging ; Horses ; Retina/diagnostic imaging ; Tomography, Optical Coherence/veterinary
    Language English
    Publishing date 2021-11-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 390796-x
    ISSN 1943-5681 ; 0002-9645
    ISSN (online) 1943-5681
    ISSN 0002-9645
    DOI 10.2460/ajvr.21.02.0029
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 422: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Myristoylated Alanine Rich C Kinase Substrate (MARCKS) is essential to β2-integrin dependent responses of equine neutrophils.

    Sheats, Mary K / Pescosolido, Kimberly C / Hefner, Ethan M / Sung, Eui Jae / Adler, Kenneth B / Jones, Samuel L

    Veterinary immunology and immunopathology

    2014  Volume 160, Issue 3-4, Page(s) 167–176

    Abstract: Neutrophil infiltration is a prominent feature in a number of pathologic conditions affecting horses including recurrent airway obstruction, ischemia-reperfusion injury, and laminitis. Cell signaling components involved in neutrophil migration represent ... ...

    Abstract Neutrophil infiltration is a prominent feature in a number of pathologic conditions affecting horses including recurrent airway obstruction, ischemia-reperfusion injury, and laminitis. Cell signaling components involved in neutrophil migration represent targets for novel anti-inflammatory therapies. In order to migrate into tissue, neutrophils must respond to chemoattractant signals in their external environment through activation of adhesion receptors (i.e. integrins) and reorganization of the actin cytoskeleton. Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS), a highly conserved actin-binding protein, has a well demonstrated role in cytoskeletal dependent cellular functions (i.e. adhesion, spreading, and migration), but the details of MARCKS involvement in these processes remain vague. We hypothesized that MARCKS serves as a link between the actin cytoskeleton and integrin function in neutrophils. Using a MARCKS-specific inhibitor peptide known as MANS on equine neutrophils in vitro, we demonstrate that inhibition of MARCKS function significantly attenuates β2-integrin-dependent neutrophil functions including migration, adhesion, and immune complex-mediated respiratory burst. The MANS peptide did not, however, inhibit the β2-integrin-independent PMA mediated respiratory burst. These results attest to the essential role of MARCKS function in regulating neutrophil responses, and strongly implicate MARCKS as a potential regulator of β2-integrins in neutrophils.
    MeSH term(s) Amino Acid Sequence ; Animals ; Antigen-Antibody Complex/physiology ; CD18 Antigens/immunology ; CD18 Antigens/physiology ; Cell Adhesion/drug effects ; Cell Adhesion/physiology ; Cell Movement/drug effects ; Cell Movement/physiology ; Conserved Sequence ; Horses/immunology ; Horses/physiology ; In Vitro Techniques ; Intracellular Signaling Peptides and Proteins/antagonists & inhibitors ; Intracellular Signaling Peptides and Proteins/genetics ; Intracellular Signaling Peptides and Proteins/physiology ; Membrane Proteins/antagonists & inhibitors ; Membrane Proteins/genetics ; Membrane Proteins/physiology ; Molecular Sequence Data ; Myristoylated Alanine-Rich C Kinase Substrate ; Neutrophil Infiltration/drug effects ; Neutrophil Infiltration/immunology ; Neutrophil Infiltration/physiology ; Peptide Fragments/genetics ; Peptide Fragments/pharmacology ; Respiratory Burst/drug effects ; Sequence Homology, Amino Acid ; Tetradecanoylphorbol Acetate/pharmacology
    Chemical Substances Antigen-Antibody Complex ; CD18 Antigens ; Intracellular Signaling Peptides and Proteins ; MARCKS-related peptide ; Membrane Proteins ; Peptide Fragments ; Myristoylated Alanine-Rich C Kinase Substrate (125267-21-2) ; Tetradecanoylphorbol Acetate (NI40JAQ945)
    Language English
    Publishing date 2014-05-02
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 754160-0
    ISSN 1873-2534 ; 0165-2427
    ISSN (online) 1873-2534
    ISSN 0165-2427
    DOI 10.1016/j.vetimm.2014.04.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2177: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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