LIVIVO - Search results -

Search results

Result 1 - 10 of total 12

Search options

Article ; Online: Multidrug-resistant toxigenic

Arcari, Gabriele / Hennart, Mélanie / Badell, Edgar / Brisse, Sylvain

2023 Volume 9, Issue 1

Abstract: Antimicrobial therapy is important for case management of diphtheria, but knowledge on the emergence of multidrug-resistance ... ...

Abstract	Antimicrobial therapy is important for case management of diphtheria, but knowledge on the emergence of multidrug-resistance in
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Corynebacterium diphtheriae/drug effects ; Corynebacterium diphtheriae/genetics ; Genomic Islands ; Drug Resistance, Multiple, Bacterial
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2023-02-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2835258-0
ISSN	2057-5858 ; 2057-5858
ISSN (online)	2057-5858
ISSN	2057-5858
DOI	10.1099/mgen.0.000923
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Multidrug-resistant

Brémont, Sylvie / Passet, Virginie / Hennart, Mélanie / Fonteneau, Laure / Toubiana, Julie / Badell, Edgar / Brisse, Sylvain

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin

2023 Volume 28, Issue 46

Abstract: We describe 10 unlinked cases ... ...

Abstract	We describe 10 unlinked cases of
MeSH term(s)	Humans ; Corynebacterium diphtheriae/genetics ; Diphtheria/diagnosis ; Diphtheria/drug therapy ; Diphtheria/epidemiology ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; France/epidemiology ; Mali
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2023-11-16
Publishing country	Sweden
Document type	Journal Article
ZDB-ID	1338803-4
ISSN	1560-7917 ; 1025-496X
ISSN (online)	1560-7917
ISSN	1025-496X
DOI	10.2807/1560-7917.ES.2023.28.46.2300615
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5066: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Corynebacteria of the

Museux, Kristina / Arcari, Gabriele / Rodrigo, Guido / Hennart, Melanie / Badell, Edgar / Toubiana, Julie / Brisse, Sylvain

Microbiology spectrum

2023 Volume 11, Issue 3, Page(s) e0000623

Abstract: Corynebacteria of ... ...

Abstract	Corynebacteria of the
MeSH term(s)	Humans ; Animals ; Dogs ; Horses ; Rats ; Rhinitis ; Corynebacterium diphtheriae ; Diphtheria/microbiology ; Anti-Bacterial Agents/pharmacology ; France/epidemiology ; Mammals
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2023-04-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.00006-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Genomic Epidemiology and Strain Taxonomy of

Guglielmini, Julien / Hennart, Melanie / Badell, Edgar / Toubiana, Julie / Criscuolo, Alexis / Brisse, Sylvain

Journal of clinical microbiology

2021 Volume 59, Issue 12, Page(s) e0158121

Abstract: Corynebacterium diphtheriae is highly transmissible and can cause large diphtheria outbreaks where vaccination coverage is insufficient. Sporadic cases or small clusters are observed in high-vaccination settings. The phylogeography and short timescale ... ...

Abstract	Corynebacterium diphtheriae is highly transmissible and can cause large diphtheria outbreaks where vaccination coverage is insufficient. Sporadic cases or small clusters are observed in high-vaccination settings. The phylogeography and short timescale evolution of C. diphtheriae are not well understood, in part due to a lack of harmonized analytical approaches of genomic surveillance and strain tracking. We combined 1,305 genes with highly reproducible allele calls into a core genome multilocus sequence typing (cgMLST) scheme. We analyzed cgMLST gene diversity among 602 isolates from sporadic clinical cases, small clusters, or large outbreaks. We defined sublineages based on the phylogenetic structure within C. diphtheriae and strains based on the highest number of cgMLST mismatches within documented outbreaks. We performed time-scaled phylogenetic analyses of major sublineages. The cgMLST scheme showed high allele call rate in C. diphtheriae and the closely related species C. belfantii and C. rouxii. We demonstrate its utility to delineate epidemiological case clusters and outbreaks using a 25 mismatches threshold and reveal a number of cryptic transmission chains, most of which are geographically restricted to one or a few adjacent countries. Subcultures of the vaccine strain PW8 differed by up to 20 cgMLST mismatches. Phylogenetic analyses revealed a short-timescale evolutionary gain or loss of the diphtheria toxin and biovar-associated genes. We devised a genomic taxonomy of strains and deeper sublineages (defined using a 500-cgMLST-mismatch threshold), currently comprising 151 sublineages, only a few of which are geographically widespread based on current sampling. The cgMLST genotyping tool and nomenclature was made publicly accessible (https://bigsdb.pasteur.fr/diphtheria). Standardized genome-scale strain genotyping will help tracing transmission and geographic spread of C. diphtheriae. The unified genomic taxonomy of C. diphtheriae strains provides a common language for studies of ecology, evolution, and virulence heterogeneity among C. diphtheriae sublineages.
MeSH term(s)	Corynebacterium diphtheriae/genetics ; Diphtheria/epidemiology ; Diphtheria/microbiology ; Genome, Bacterial ; Genomics ; Humans ; Multilocus Sequence Typing ; Phylogeny
Language	English
Publishing date	2021-09-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.01581-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1188: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: A Dual Barcoding Approach to Bacterial Strain Nomenclature: Genomic Taxonomy of Klebsiella pneumoniae Strains.

Hennart, Melanie / Guglielmini, Julien / Bridel, Sébastien / Maiden, Martin C J / Jolley, Keith A / Criscuolo, Alexis / Brisse, Sylvain

Molecular biology and evolution

2022 Volume 39, Issue 7

Abstract: Sublineages (SLs) within microbial species can differ widely in their ecology and pathogenicity, and their precise definition is important in basic research and for industrial or public health applications. Widely accepted strategies to define SLs are ... ...

Abstract	Sublineages (SLs) within microbial species can differ widely in their ecology and pathogenicity, and their precise definition is important in basic research and for industrial or public health applications. Widely accepted strategies to define SLs are currently missing, which confuses communication in population biology and epidemiological surveillance. Here, we propose a broadly applicable genomic classification and nomenclature approach for bacterial strains, using the prominent public health threat Klebsiella pneumoniae as a model. Based on a 629-gene core genome multilocus sequence typing (cgMLST) scheme, we devised a dual barcoding system that combines multilevel single linkage (MLSL) clustering and life identification numbers (LINs). Phylogenetic and clustering analyses of >7,000 genome sequences captured population structure discontinuities, which were used to guide the definition of 10 infraspecific genetic dissimilarity thresholds. The widely used 7-gene multilocus sequence typing (MLST) nomenclature was mapped onto MLSL SLs (threshold: 190 allelic mismatches) and clonal group (threshold: 43) identifiers for backwards nomenclature compatibility. The taxonomy is publicly accessible through a community-curated platform (https://bigsdb.pasteur.fr/klebsiella), which also enables external users' genomic sequences identification. The proposed strain taxonomy combines two phylogenetically informative barcode systems that provide full stability (LIN codes) and nomenclatural continuity with previous nomenclature (MLSL). This species-specific dual barcoding strategy for the genomic taxonomy of microbial strains is broadly applicable and should contribute to unify global and cross-sector collaborative knowledge on the emergence and microevolution of bacterial pathogens.
MeSH term(s)	Genome, Bacterial ; Genomics ; Genotype ; Klebsiella pneumoniae/genetics ; Multilocus Sequence Typing ; Phylogeny
Language	English
Publishing date	2022-06-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	998579-7
ISSN	1537-1719 ; 0737-4038
ISSN (online)	1537-1719
ISSN	0737-4038
DOI	10.1093/molbev/msac135
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2137: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Genomic Epidemiology of Corynebacterium diphtheriae in New Caledonia.

Tessier, Eve / Hennart, Melanie / Badell, Edgar / Passet, Virginie / Toubiana, Julie / Biron, Antoine / Gourinat, Ann-Claire / Merlet, Audrey / Colot, Julien / Brisse, Sylvain

Microbiology spectrum

2023 Volume 11, Issue 3, Page(s) e0461622

Abstract: An increasing number of isolations of Corynebacterium diphtheriae has been observed in recent years in the archipelago of New Caledonia. We aimed to analyze the clinical and microbiological features of samples with C. diphtheriae. All C. diphtheriae ... ...

Abstract	An increasing number of isolations of Corynebacterium diphtheriae has been observed in recent years in the archipelago of New Caledonia. We aimed to analyze the clinical and microbiological features of samples with C. diphtheriae. All C. diphtheriae isolates identified in New Caledonia from May 2015 to May 2019 were included. For each case, a retrospective consultation of the patient files was conducted. Antimicrobial susceptibility phenotypes,
MeSH term(s)	Female ; Humans ; Corynebacterium diphtheriae/genetics ; Diphtheria/epidemiology ; Diphtheria/microbiology ; Multilocus Sequence Typing ; New Caledonia/epidemiology ; Retrospective Studies ; Corynebacterium/genetics ; Genomics ; Anti-Bacterial Agents/pharmacology ; Tetracycline ; Protein Synthesis Inhibitors
Chemical Substances	Anti-Bacterial Agents ; Tetracycline (F8VB5M810T) ; Protein Synthesis Inhibitors
Language	English
Publishing date	2023-04-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.04616-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: A global Corynebacterium diphtheriae genomic framework sheds light on current diphtheria reemergence

Hennart, Mélanie / Crestani, Chiara / Bridel, Sébastien / Armatys, Nathalie / Brémont, Sylvie / Carmi-Leroy, Annick / Landier, Annie / Passet, Virginie / Fonteneau, Laure / Vaux, Sophie / Toubiana, Julie / Badell, Edgar / Brisse, Sylvain

Peer Community Journal, Vol 3, Iss , Pp - (2023)

2023

Abstract: Background: Diphtheria, caused by Corynebacterium diphtheriae, reemerges in Europe since 2022. Genomic sequencing can inform on transmission routes and genotypes of concern, but currently, no standard approach exists to detect clinically important ... ...

Abstract	Background: Diphtheria, caused by Corynebacterium diphtheriae, reemerges in Europe since 2022. Genomic sequencing can inform on transmission routes and genotypes of concern, but currently, no standard approach exists to detect clinically important genomic features and to interpret emergence in the global C. diphtheriae population framework. Methods: We developed the bioinformatics pipeline diphtOscan (available at https://gitlab.pasteur.fr/BEBP/diphtoscan) to extract from genomes of Corynebacteria of the diphtheriae species complex, medically relevant features including tox gene presence and disruption. We analyzed 101 human C. diphtheriae isolates collected in 2022 in metropolitan and overseas France (France-2022). To define the population background of this emergence, we sequenced 379 additional isolates (mainly from France, 2018-2021) and collated 870 publicly-available genomes. Results: The France-2022 isolates comprised 45 tox-positive (44 toxigenic) isolates, mostly imported, belonging to 10 sublineages (<500 distinct core genes). The global dataset comprised 245 sublineages and 33.9% tox-positive genomes, with diphtOscan predicting non-toxigenicity in 16.0% of these. 12% of the global isolates, and 43.6% of France-2022 ones, were multidrug resistant. Convergence of toxigenicity with penicillin and erythromycin resistance was observed in 2 isolates from France-2022. Phylogenetic lineages Gravis and Mitis contrasted strikingly in their pathogenicity-associated genes. Conclusions: This work provides a bioinformatics tool and global population framework to analyze C. diphtheriae genomes, revealing important heterogeneities in virulence and resistance features. Emerging genotypes combining toxigenicity and first-line antimicrobial resistance represent novel threats. Genomic epidemiology studies of C. diphtheriae should be intensified globally to improve understanding of reemergence and spatial spread.
Keywords	diphtheria ; genomic sequencing ; antimicrobial resistance ; virulence ; epidemiology ; transmission ; 2022 reemergence ; bioinformatics tool ; Archaeology ; CC1-960 ; Science ; Q
Language	English
Publishing date	2023-08-01T00:00:00Z
Publisher	Peer Community In
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Corynebacterium rouxii sp. nov., a novel member of the diphtheriae species complex

Badell, Edgar / Hennart, Mélanie / Rodrigues, Carla / Passet, Virginie / Dazas, Melody / Panunzi, Leonardo / Bouchez, Valérie / Carmi-Leroy, Annick / Toubiana, Julie / Brisse, Sylvain

Research in microbiology. 2020 Feb. 19,

2020

Abstract: A group of six clinical isolates previously identified as Corynebacterium diphtheriae biovar Belfanti, isolated from human cutaneous or peritoneum infections and from one dog, were characterized by genomic sequencing, biochemical analysis and MALDI-TOF ... ...

Abstract	A group of six clinical isolates previously identified as Corynebacterium diphtheriae biovar Belfanti, isolated from human cutaneous or peritoneum infections and from one dog, were characterized by genomic sequencing, biochemical analysis and MALDI-TOF mass spectrometry. The six isolates were negative for the diphtheria toxin gene. Phylogenetic analyses showed that the six isolates (including FRC0190T) are clearly demarcated from C. diphtheriae, C. belfantii, C. ulcerans and C. pseudotuberculosis. The average nucleotide identity of FRC0190T with C. diphtheriae NCTC11397T was 92.6%, and was 91.8% with C. belfantii FRC0043T. C. diphtheriae subsp. lausannense strain CHUV2995T appeared to be a later heterotypic synonym of C. belfantii (ANI, 99.3%). Phenotyping data revealed an atypical negative or heterogeneous intermediate maltose fermentation reaction for the six isolates. MALDI-TOF mass spectrometry differentiated the new group from the other Corynebacterium taxa by the presence of specific spectral peaks. rpoB sequences showed identity to atypical, maltose-negative C. diphtheriae biovar Belfanti isolates previously described from two cats in the USA. We propose the name Corynebacterium rouxii sp. nov. for the novel group, with FRC0190T (= CIP 111752T = DSM 110354T) as type strain.
Keywords	Corynebacterium diphtheriae ; Corynebacterium pseudotuberculosis ; Corynebacterium ulcerans ; cats ; diphtheria toxin ; dogs ; fermentation ; genes ; humans ; maltose ; matrix-assisted laser desorption-ionization mass spectrometry ; new species ; peritoneum ; phenotype ; phylogeny ; sequence analysis ; United States
Language	English
Dates of publication	2020-0219
Publishing place	Elsevier Masson SAS
Document type	Article
Note	Pre-press version
ZDB-ID	1004220-9
ISSN	1769-7123 ; 0923-2508
ISSN (online)	1769-7123
ISSN	0923-2508
DOI	10.1016/j.resmic.2020.02.003
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 63/179 A/B: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Population genomics and antimicrobial resistance in Corynebacterium diphtheriae.

Hennart, Melanie / Panunzi, Leonardo G / Rodrigues, Carla / Gaday, Quentin / Baines, Sarah L / Barros-Pinkelnig, Marina / Carmi-Leroy, Annick / Dazas, Melody / Wehenkel, Anne Marie / Didelot, Xavier / Toubiana, Julie / Badell, Edgar / Brisse, Sylvain

Genome medicine

2020 Volume 12, Issue 1, Page(s) 107

Abstract: Background: Corynebacterium diphtheriae, the agent of diphtheria, is a genetically diverse bacterial species. Although antimicrobial resistance has emerged against several drugs including first-line penicillin, the genomic determinants and population ... ...

Abstract	Background: Corynebacterium diphtheriae, the agent of diphtheria, is a genetically diverse bacterial species. Although antimicrobial resistance has emerged against several drugs including first-line penicillin, the genomic determinants and population dynamics of resistance are largely unknown for this neglected human pathogen. Methods: Here, we analyzed the associations of antimicrobial susceptibility phenotypes, diphtheria toxin production, and genomic features in C. diphtheriae. We used 247 strains collected over several decades in multiple world regions, including the 163 clinical isolates collected prospectively from 2008 to 2017 in France mainland and overseas territories. Results: Phylogenetic analysis revealed multiple deep-branching sublineages, grouped into a Mitis lineage strongly associated with diphtheria toxin production and a largely toxin gene-negative Gravis lineage with few toxin-producing isolates including the 1990s ex-Soviet Union outbreak strain. The distribution of susceptibility phenotypes allowed proposing ecological cutoffs for most of the 19 agents tested, thereby defining acquired antimicrobial resistance. Penicillin resistance was found in 17.2% of prospective isolates. Seventeen (10.4%) prospective isolates were multidrug-resistant (≥ 3 antimicrobial categories), including four isolates resistant to penicillin and macrolides. Homologous recombination was frequent (r/m = 5), and horizontal gene transfer contributed to the emergence of antimicrobial resistance in multiple sublineages. Genome-wide association mapping uncovered genetic factors of resistance, including an accessory penicillin-binding protein (PBP2m) located in diverse genomic contexts. Gene pbp2m is widespread in other Corynebacterium species, and its expression in C. glutamicum demonstrated its effect against several beta-lactams. A novel 73-kb C. diphtheriae multiresistance plasmid was discovered. Conclusions: This work uncovers the dynamics of antimicrobial resistance in C. diphtheriae in the context of phylogenetic structure, biovar, and diphtheria toxin production and provides a blueprint to analyze re-emerging diphtheria.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Corynebacterium diphtheriae/classification ; Corynebacterium diphtheriae/drug effects ; Corynebacterium diphtheriae/genetics ; Corynebacterium diphtheriae/isolation & purification ; DNA, Bacterial/genetics ; Diphtheria/microbiology ; Diphtheria Toxin/genetics ; Drug Resistance, Bacterial/genetics ; Genes, Bacterial/genetics ; Genome-Wide Association Study ; Genomics ; Humans ; Macrolides/pharmacology ; Metagenomics ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; Phylogeny ; Prospective Studies
Chemical Substances	Anti-Bacterial Agents ; DNA, Bacterial ; Diphtheria Toxin ; Macrolides
Language	English
Publishing date	2020-11-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2484394-5
ISSN	1756-994X ; 1756-994X
ISSN (online)	1756-994X
ISSN	1756-994X
DOI	10.1186/s13073-020-00805-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Corynebacterium rouxii sp. nov., a novel member of the diphtheriae species complex.

Badell, Edgar / Hennart, Mélanie / Rodrigues, Carla / Passet, Virginie / Dazas, Melody / Panunzi, Leonardo / Bouchez, Valérie / Carmi-Leroy, Annick / Toubiana, Julie / Brisse, Sylvain

Research in microbiology

2020 Volume 171, Issue 3-4, Page(s) 122–127

Abstract	A group of six clinical isolates previously identified as Corynebacterium diphtheriae biovar Belfanti, isolated from human cutaneous or peritoneum infections and from one dog, were characterized by genomic sequencing, biochemical analysis and MALDI-TOF mass spectrometry. The six isolates were negative for the diphtheria toxin gene. Phylogenetic analyses showed that the six isolates (including FRC0190
MeSH term(s)	Bacterial Typing Techniques ; Corynebacterium/chemistry ; Corynebacterium/classification ; Corynebacterium/genetics ; Corynebacterium Infections/microbiology ; Corynebacterium diphtheriae/chemistry ; Corynebacterium diphtheriae/classification ; Corynebacterium diphtheriae/genetics ; Humans ; Phylogeny ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Whole Genome Sequencing
Language	English
Publishing date	2020-02-28
Publishing country	France
Document type	Journal Article
ZDB-ID	1004220-9
ISSN	1769-7123 ; 0923-2508
ISSN (online)	1769-7123
ISSN	0923-2508
DOI	10.1016/j.resmic.2020.02.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 890: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito