LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 114

Search options

  1. Book ; Online: Le Mort dans la ville : Pratiques, contextes et impacts des inhumations intra-muros en Anatolie, du début de lAge du Bronze à lépoque romaine

    Henry, Olivier

    2013  

    Abstract: Le développement de rites mortuaires complexes dans l'histoire de l'Homme a résulté dans un rôle croissant joué par les pratiques funéraires utilisées comme moyen de resserrer les liens à l'intérieur d'une même communauté. À cet égard, le singulier usage ...

    Abstract Le développement de rites mortuaires complexes dans l'histoire de l'Homme a résulté dans un rôle croissant joué par les pratiques funéraires utilisées comme moyen de resserrer les liens à l'intérieur d'une même communauté. À cet égard, le singulier usage dinhumer un individu au cœur de la communauté révèle avec acuité la force de cette relation que pouvaient entretenir les vivants et les morts. Les découvertes archéologiques récentes ont souligné l'importance de telles pratiques liées aux inhumations intra-muros en Anatolie. Bien qu'il semble possible de tisser un lien continu entre ces coutumes, les contextes dans lesquels s'inscrivent la pratique d'inhumer une personne au cœur même de la communauté, depuis l'enfant du Néolithique à Çatalhöyük à la libraire de Celsius à Ephèse, en passant par le Mausolée dHalicarnasse, ont néanmoins radicalement changés en fonction des époques et des lieux. L'objectif de ce volume, en rassemblant des spécialistes de périodes et d'horizons différents, est d'offrir non seulement un point général de nos connaissances sur ces questions, mais aussi un éclairage concernant le mécanisme de ces pratiques, leur contexte et leur impact en Anatolie, du début de l'Âge du Bronze à l'époque romaine
    Keywords Social sciences (General)
    Size 1 electronic resource ( pages)
    Publisher Institut français d'études anatoliennes
    Document type Book ; Online
    Note French ; Open Access
    HBZ-ID HT020321549
    ISBN 9782362450556 ; 2362450554
    DOI 10.4000/books.ifeagd.2057
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    More links

    Kategorien

  2. Book ; Online: Archéologies et espaces parcourus

    Henry, Olivier

    2012  

    Size 1 electronic resource ( p.)
    Publisher Institut français d'études anatoliennes
    Document type Book ; Online
    Note French ; Open Access
    HBZ-ID HT020094030
    ISBN 9782362450068 ; 9782362450549 ; 2362450066 ; 2362450546
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    More links

    Kategorien

  3. Book ; Online: Considérer la mort: de la protection des tombes dans l'antiquité à leur conservation aujourd'hui

    Henry, Olivier

    2003  

    Size 1 electronic resource ( p.)
    Publisher Institut français d'études anatoliennes
    Document type Book ; Online
    Note French ; Open Access
    HBZ-ID HT020094034
    ISBN 9782906053793 ; 9782362450365 ; 2906053791 ; 2362450368
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    More links

    Kategorien

  4. Article ; Online: Antibody-independent surface plasmon resonance assays for influenza vaccine quality control.

    Serafin, Benjamin / Kamen, Amine / de Crescenzo, Gregory / Henry, Olivier

    Applied microbiology and biotechnology

    2024  Volume 108, Issue 1, Page(s) 307

    Abstract: Surface plasmon resonance (SPR)-based biosensors have emerged as a powerful platform for bioprocess monitoring due to their ability to detect biointeractions in real time, without the need for labeling. Paramount for the development of a robust detection ...

    Abstract Surface plasmon resonance (SPR)-based biosensors have emerged as a powerful platform for bioprocess monitoring due to their ability to detect biointeractions in real time, without the need for labeling. Paramount for the development of a robust detection platform is the immobilization of a ligand with high specificity and affinity for the in-solution species of interest. Following the 2009 H1N1 pandemic, much effort has been made toward the development of quality control platforms for influenza A vaccine productions, many of which have employed SPR for detection. Due to the rapid antigenic drift of influenza's principal surface protein, hemagglutinin, antibodies used for immunoassays need to be produced seasonally. The production of these antibodies represents a 6-8-week delay in immunoassay and, thus, vaccine availability. This review focuses on SPR-based assays that do not rely on anti-HA antibodies for the detection, characterization, and quantification of influenza A in bioproductions and biological samples. KEY POINTS: • The single radial immunodiffusion assay (SRID) has been the gold standard for the quantification of influenza vaccines since 1979. Due to antigenic drift of influenza's hemagglutinin protein, new antibody reagents for the SRID assay must be produced each year, requiring 6-8 weeks. The resulting delay in immunoassay availability is a major bottleneck in the influenza vaccine pipeline. This review highlights ligand options for the detection and quantification of influenza viruses using surface plasmon resonance biosensors.
    MeSH term(s) Surface Plasmon Resonance/methods ; Influenza Vaccines/immunology ; Humans ; Quality Control ; Antibodies, Viral/immunology ; Hemagglutinin Glycoproteins, Influenza Virus/immunology ; Influenza, Human/diagnosis ; Influenza, Human/prevention & control ; Influenza, Human/immunology ; Immunoassay/methods ; Immunoassay/standards ; Biosensing Techniques/methods ; Influenza A virus/immunology
    Chemical Substances Influenza Vaccines ; Antibodies, Viral ; Hemagglutinin Glycoproteins, Influenza Virus
    Language English
    Publishing date 2024-04-24
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-024-13145-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Book ; Online: La Cappadoce méridionale de la Préhistoire à lépoque byzantine : 3e Rencontres darchéologie de IFEA, Istanbul 8-9 novembre 2012

    Tibet, Aksel / Henry, Olivier / Beyer, Dominique

    2012  

    Abstract: Il y a environ 25 ans, Olivier Pelon organisait à l'Institut Français d'Études Anatoliennes d'Istanbul un colloque destiné à faire l'état des recherches sur la Cappadoce méridionale jusqu'à la fin de l'époque romaine. Un quart de siècle après ce premier ... ...

    Abstract Il y a environ 25 ans, Olivier Pelon organisait à l'Institut Français d'Études Anatoliennes d'Istanbul un colloque destiné à faire l'état des recherches sur la Cappadoce méridionale jusqu'à la fin de l'époque romaine. Un quart de siècle après ce premier colloque, il était intéressant de faire un nouveau point sur l'avancée des recherches dans cette Cappadoce méridionale, de la préhistoire à la période byzantine. Ce nouveau colloque, placé cette fois encore sous l'égide de l'Institut Français d'Études Anatoliennes et intégré à la série des Rencontres d'archéologie de l'IFEA réunit vingt-trois communications. Si les périodes néolithique et chalcolithique ont été particulièrement bien représentées, ce qui témoigne bien de l'importance de cette phase de la préhistoire cappadocienne, liée aux gisements d'obsidienne des Melendiz Dağları, on soulignera en revanche l'absence presque totale du Bronze Ancien. Cette phase est en effet peu représentée dans l'archéologie locale. La même remarque peut s'appliquer au Bronze Moyen. La fin du Bronze Moyen, fort heureusement, est représentée à Porsuk, de même que le Bronze Récent qui bénéficie, depuis peu, tout comme l'Âge du Fer, du démarrage fructueux des fouilles de Kınık Höyük. Enfin, l'Antiquité tardive et Byzance ont pu être représentées, principalement autour de Tyane, ce qui n'avait pas pu être le cas lors du premier colloque. En octobre 2012, quelques semaines avant la tenue de la Rencontre, on apprenait malheureusement le décès brutal et inattendu d'Olivier Pelon, ancien directeur de la mission de Porsuk (jusqu'en 2002) et organisateur de ce premier colloque cappadocien. C'est bien en hommage à sa mémoire que notre Rencontre cappadocienne de 2012 et sa publication ont été naturellement dédiées
    Keywords Social sciences (General)
    Size 1 electronic resource ( pages)
    Publisher Institut français d'études anatoliennes
    Document type Book ; Online
    Note French ; Open Access
    HBZ-ID HT020677005
    ISBN 9782362450822 ; 2362450821
    DOI 10.4000/books.ifeagd.3187
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    More links

    Kategorien

  6. Article: Intensified Influenza Virus Production in Suspension HEK293SF Cell Cultures Operated in Fed-Batch or Perfusion with Continuous Harvest.

    Silva, Cristina A T / Kamen, Amine A / Henry, Olivier

    Vaccines

    2023  Volume 11, Issue 12

    Abstract: Major efforts in the intensification of cell culture-based viral vaccine manufacturing focus on the development of high-cell-density (HCD) processes, often operated in perfusion. While perfusion operations allow for higher viable cell densities and ... ...

    Abstract Major efforts in the intensification of cell culture-based viral vaccine manufacturing focus on the development of high-cell-density (HCD) processes, often operated in perfusion. While perfusion operations allow for higher viable cell densities and volumetric productivities, the high perfusion rates (PR) normally adopted-typically between 2 and 4 vessel volumes per day (VVD)-dramatically increase media consumption, resulting in a higher burden on the cell retention device and raising challenges for the handling and disposal of high volumes of media. In this study, we explore high inoculum fed-batch (HIFB) and low-PR perfusion operations to intensify a cell culture-based process for influenza virus production while minimizing media consumption. To reduce product retention time in the bioreactor, produced viral particles were continuously harvested using a tangential flow depth filtration (TFDF) system as a cell retention device and harvest unit. The feeding strategies developed-a hybrid fed-batch with continuous harvest and a low-PR perfusion-allowed for infections in the range of 8-10 × 10
    Language English
    Publishing date 2023-12-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines11121819
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  7. Article ; Online: A low-temperature SPR-based assay for monoclonal antibody galactosylation and fucosylation assessment using FcγRIIA/B.

    Gaudreault, Jimmy / Forest-Nault, Catherine / Gilbert, Michel / Durocher, Yves / Henry, Olivier / De Crescenzo, Gregory

    Biotechnology and bioengineering

    2024  Volume 121, Issue 5, Page(s) 1659–1673

    Abstract: Monoclonal antibodies (MAbs) are powerful therapeutic tools in modern medicine and represent a rapidly expanding multibillion USD market. While bioprocesses are generally well understood and optimized for MAbs, online quality control remains challenging. ...

    Abstract Monoclonal antibodies (MAbs) are powerful therapeutic tools in modern medicine and represent a rapidly expanding multibillion USD market. While bioprocesses are generally well understood and optimized for MAbs, online quality control remains challenging. Notably, N-glycosylation is a critical quality attribute of MAbs as it affects binding to Fcγ receptors (FcγRs), impacting the efficacy and safety of MAbs. Traditional N-glycosylation characterization methods are ill-suited for online monitoring of a bioreactor; in contrast, surface plasmon resonance (SPR) represents a promising avenue, as SPR biosensors can record MAb-FcγR interactions in real-time and without labeling. In this study, we produced five lots of differentially glycosylated Trastuzumab (TZM) and finely characterized their glycosylation profile by HILIC-UPLC chromatography. We then compared the interaction kinetics of these MAb lots with four FcγRs including FcγRIIA and FcγRIIB at 5°C and 25°C. When interacting with FcγRIIA/B at low temperature, the differentially glycosylated MAb lots exhibited distinct kinetic behaviors, contrary to room-temperature experiments. Galactosylated TZM (1) and core fucosylated TZM (2) could be discriminated and even quantified using an analytical technique based on the area under the curve of the signal recorded during the dissociation phase of a SPR sensorgram describing the interaction with FcγRIIA (1) or FcγRII2B (2). Because of the rapidity of the proposed method (<5 min per measurement) and the small sample concentration it requires (as low as 30 nM, exact concentration not required), it could be a valuable process analytical technology for MAb glycosylation monitoring.
    MeSH term(s) Antibodies, Monoclonal/chemistry ; Receptors, IgG/metabolism ; Surface Plasmon Resonance ; Glycosylation ; Temperature ; Trastuzumab
    Chemical Substances Antibodies, Monoclonal ; Fc gamma receptor IIA ; Receptors, IgG ; Trastuzumab (P188ANX8CK)
    Language English
    Publishing date 2024-02-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 280318-5
    ISSN 1097-0290 ; 0006-3592
    ISSN (online) 1097-0290
    ISSN 0006-3592
    DOI 10.1002/bit.28673
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  8. Article ; Online: Multi-temperature experiments to ease analysis of heterogeneous binder solutions by surface plasmon resonance biosensing.

    Gaudreault, Jimmy / Durocher, Yves / Henry, Olivier / De Crescenzo, Gregory

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 14401

    Abstract: Surface Plasmon Resonance (SPR) biosensing is a well-established tool for the investigation of binding kinetics between a soluble species and an immobilized (bio)molecule. While robust and accurate data analysis techniques are readily available for ... ...

    Abstract Surface Plasmon Resonance (SPR) biosensing is a well-established tool for the investigation of binding kinetics between a soluble species and an immobilized (bio)molecule. While robust and accurate data analysis techniques are readily available for single species, methods to exploit data collected with a solution containing multiple interactants are scarce. In a previous study, our group proposed two data analysis algorithms for (1) the precise and reliable identification of the kinetic parameters of N interactants present at different ratios in N mixtures and (2) the estimation of the composition of a given mixture, assuming that the kinetic parameters and the total concentration of all interactants are known. Here, we extend the first algorithm by reducing the number of necessary mixtures. This is achieved by conducting experiments at different temperatures. Through the Van't Hoff and Eyring equations, identifying the kinetic and thermodynamic parameters of N binders becomes possible with M mixtures with M comprised between 2 and N and at least N/M temperatures. The second algorithm is improved by adding the total analyte concentration as a supplementary variable to be identified in an optimization routine. We validated our analysis framework experimentally with a system consisting of mixtures of low molecular weight drugs, each competing to bind to an immobilized protein. We believe that the analysis of mixtures and composition estimation could pave the way for SPR biosensing to become a bioprocess monitoring tool, on top of expanding its already substantial role in drug discovery and development.
    MeSH term(s) Biosensing Techniques ; Kinetics ; Proteins/metabolism ; Surface Plasmon Resonance/methods ; Temperature ; Thermodynamics
    Chemical Substances Proteins
    Language English
    Publishing date 2022-08-24
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-18450-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  9. Article ; Online: Seasonal Reproduction in Neotropical Rainforest Mammals.

    Dubost, Gérard / Henry, Olivier

    Zoological studies

    2017  Volume 56, Page(s) e2

    Abstract: Gérard Dubost, and Olivier Henry (2017) ...

    Abstract Gérard Dubost, and Olivier Henry (2017)
    Language English
    Publishing date 2017-01-17
    Publishing country China (Republic : 1949- )
    Document type Journal Article
    ZDB-ID 2105348-0
    ISSN 1810-522X ; 1021-5506
    ISSN (online) 1810-522X
    ISSN 1021-5506
    DOI 10.6620/ZS.2017.56-02
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article ; Online: A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2 Receptor Binding Domain.

    Forest-Nault, Catherine / Koyuturk, Izel / Gaudreault, Jimmy / Pelletier, Alex / L'Abbé, Denis / Cass, Brian / Bisson, Louis / Burlacu, Alina / Delafosse, Laurence / Stuible, Matthew / Henry, Olivier / De Crescenzo, Gregory / Durocher, Yves

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2762, Page(s) 89–105

    Abstract: Surface plasmon resonance (SPR)-based biosensing enables the characterization of protein-protein interactions. Several SPR-based approaches have been designed to evaluate the binding mechanism between the angiotensin-converting enzyme 2 (ACE2) receptor ... ...

    Abstract Surface plasmon resonance (SPR)-based biosensing enables the characterization of protein-protein interactions. Several SPR-based approaches have been designed to evaluate the binding mechanism between the angiotensin-converting enzyme 2 (ACE2) receptor and the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein leading to a large range of kinetic and thermodynamic constants. This chapter describes a robust SPR assay based on the K5/E5 coiled-coil capture strategy that reduces artifacts. In this method, ACE2 receptors were produced with an E5-tag and immobilized as ligands in the SPR assay. This chapter details methods for high-yield production and purification of the studied proteins, functionalization of the sensor chip, conduction of the SPR assay, and data analysis.
    MeSH term(s) Humans ; SARS-CoV-2/metabolism ; Angiotensin-Converting Enzyme 2/metabolism ; COVID-19 ; Spike Glycoprotein, Coronavirus/metabolism ; Biosensing Techniques/methods ; Protein Binding
    Chemical Substances spike protein, SARS-CoV-2 ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23) ; Spike Glycoprotein, Coronavirus
    Language English
    Publishing date 2024-02-05
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3666-4_6
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

To top