Article ; Online: TRAK adaptors regulate the recruitment and activation of dynein and kinesin in mitochondrial transport.
2023 Volume 14, Issue 1, Page(s) 1376
Abstract: Mitochondrial transport along microtubules is mediated by Miro1 and TRAK adaptors that recruit kinesin-1 and dynein-dynactin. To understand how these opposing motors are regulated during mitochondrial transport, we reconstitute the bidirectional ... ...
| Abstract | Mitochondrial transport along microtubules is mediated by Miro1 and TRAK adaptors that recruit kinesin-1 and dynein-dynactin. To understand how these opposing motors are regulated during mitochondrial transport, we reconstitute the bidirectional transport of Miro1/TRAK along microtubules in vitro. We show that the coiled-coil domain of TRAK activates dynein-dynactin and enhances the motility of kinesin-1 activated by its cofactor MAP7. We find that TRAK adaptors that recruit both motors move towards kinesin-1's direction, whereas kinesin-1 is excluded from binding TRAK transported by dynein-dynactin, avoiding motor tug-of-war. We also test the predictions of the models that explain how mitochondrial transport stalls in regions with elevated Ca |
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| MeSH term(s) | Dyneins/metabolism ; Kinesins/metabolism ; Dynactin Complex/metabolism ; Microtubules/metabolism ; Biological Transport ; Microtubule-Associated Proteins/metabolism |
| Chemical Substances | Dyneins (EC 3.6.4.2) ; Kinesins (EC 3.6.4.4) ; Dynactin Complex ; Microtubule-Associated Proteins |
| Language | English |
| Publishing date | 2023-03-13 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. |
| ZDB-ID | 2553671-0 |
| ISSN | 2041-1723 ; 2041-1723 |
| ISSN (online) | 2041-1723 |
| ISSN | 2041-1723 |
| DOI | 10.1038/s41467-023-36945-8 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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