Article ; Online: Component-resolved diagnosis using guinea-pig allergens elucidates allergen sensitization profiles in allergy to furry animals.
2021 Volume 51, Issue 6, Page(s) 829–835
Abstract: Background: Furry animals are an important source of indoor allergens. Diagnosis of allergy to small pets such as guinea-pigs still relies on animal dander extracts which do not allow to define the primary sensitization source.: Objective: To ... ...
| Abstract | Background: Furry animals are an important source of indoor allergens. Diagnosis of allergy to small pets such as guinea-pigs still relies on animal dander extracts which do not allow to define the primary sensitization source. Objective: To identify major guinea-pig allergens and to evaluate their potential as marker allergens for in vitro IgE-diagnosis in comparison with dander extracts. Methods: A group of patients allergic to guinea-pig (n = 29) and a group of patients allergic to cat and dog (n = 30) were recruited for the study. A panel of four guinea-pig lipocalin allergens was expressed as recombinant proteins in E. coli. Specific IgE were quantified by ImmunoCAP and ELISA. Results: The combination of 4 guinea-pig lipocalin allergens, including 2 new lipocalins, Cav p 1.0201 and Cav p 6.0101, and the previously characterized lipocalins Cav p 2 and Cav p 3, enabled the identification of 90% of all patients allergic to guinea-pig. The vast majority had specific IgE to Cav p 1 (83%). Cav p 6 shares 54% sequence identity with Fel d 4 and Can f 6 and was found to be IgE-cross-reactive with these allergens. In the group of cat- and dog-allergic patients, 73% had also specific IgE to guinea-pig dander. However, only 27% of the cat /dog-allergic patients had specific IgE to any of the non-cross-reactive guinea-pig allergens Cav p 1, Cav p 2 or Cav p 3. The high prevalence of IgE to guinea-pig dander could be explained by IgE-cross-reactivity among serum albumins and certain lipocalins. Conclusions and clinical relevance: The availability of specific allergen markers is essential for the assessment of primary sensitization, especially in polysensitized patients. The proposed panel of guinea-pig allergens Cav p 1, Cav p 2 and Cav p 3 is a first step to component-resolved IgE-diagnosis of allergy to small furry pets. |
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| MeSH term(s) | Adult ; Allergens/immunology ; Animals ; Cats ; Cross Reactions/immunology ; Dander/immunology ; Dogs ; Female ; Guinea Pigs ; Humans ; Hypersensitivity/diagnosis ; Hypersensitivity/immunology ; Immunoglobulin E/immunology ; Lipocalins/immunology ; Male ; Pets |
| Chemical Substances | Allergens ; Can f 6 allergen, Canis familiaris ; Cav p 1 allergen, guinea pig ; Fel d 4 allergen, Felis domesticus ; Lipocalins ; Immunoglobulin E (37341-29-0) |
| Language | English |
| Publishing date | 2021-04-09 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 645204-8 |
| ISSN | 1365-2222 ; 0954-7894 ; 0960-2178 |
| ISSN (online) | 1365-2222 |
| ISSN | 0954-7894 ; 0960-2178 |
| DOI | 10.1111/cea.13873 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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