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  1. Article: The perils of boarding: A call to achieve parity in the delivery of acute psychiatric services for children with COVID-19.

    Jawed, Aysha / Boro-Hernandez, Rachel

    Health promotion perspectives

    2023  Volume 13, Issue 1, Page(s) 36–39

    Abstract: Boarding across pediatric healthcare systems is on the rise during the pandemic. Children with positive COVID-19 test results awaiting psychiatric placements in the emergency department or medical unit settings are at increased risk for decompensation ... ...

    Abstract Boarding across pediatric healthcare systems is on the rise during the pandemic. Children with positive COVID-19 test results awaiting psychiatric placements in the emergency department or medical unit settings are at increased risk for decompensation with unmet psychiatric needs during a time of crisis marked by vulnerability. There is scant literature unveiling best practices on delivery of care for these patients to achieve acute crisis stabilization. Recent studies have uncovered substantial increases in mental health disorders among children during the pandemic compared to previous incidence and prevalence rates prior to the pandemic. From the published literature, two healthcare systems have initiated long-term planning, development, and implementation of biodome psychiatric units for patients with COVID-19 in need of acute crisis stabilization services. We sampled 100 acute inpatient child and adolescent psychiatric programs to discern their post-COVID positive clearance policies for admission. Findings were mixed among days of quarantine required, symptomology, covid-designated spaces vs. self-isolated rooms for psychiatric treatment, number of COVID negative retests, and additional considerations. We also review a range of considerations and recommendations for clinical practice and the health system in achieving parity in mental health care for these patients which in turn could contribute towards mitigating the rising global mental health crisis. Furthermore, increasing access to acute psychiatric services for these patients will also contribute towards the larger goal of the World Health Organization, Sustainable Developmental Goals of the United Nations, and Healthy People 2030 in increasing accessibility, quality and equity of mental health care for individuals on both global and national frontiers.
    Language English
    Publishing date 2023-04-30
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2691688-5
    ISSN 2228-6497
    ISSN 2228-6497
    DOI 10.34172/hpp.2023.04
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Homeless in the hospital: A call to strengthen multidisciplinary care for children awaiting out-of-home and psychiatric placements.

    Jawed, Aysha / Boro-Hernandez, Rachel / Pickett, Diane

    Frontiers in pediatrics

    2023  Volume 10, Page(s) 1057956

    Abstract: Increasingly more school-age and adolescent children continue to await psychiatric or out-of-home placements across pediatric healthcare systems. These children comprise the growing overstay pediatric population. The medical, developmental, ... ...

    Abstract Increasingly more school-age and adolescent children continue to await psychiatric or out-of-home placements across pediatric healthcare systems. These children comprise the growing overstay pediatric population. The medical, developmental, socioemotional, behavioral and academic needs of these patients are complex and diverse. The uncertainty of waiting times for placement further continues. This growing pediatric issue emphasizes the importance of mobilizing a myriad of resources across healthcare and community contexts to support these patients during this precarious time. The reality is that there is a significant scarcity of placement resources which contributes to extended waiting times for placement. This contemporary issue brings child development, ethical and moral considerations, and healthcare operations to the forefront. We discuss a myriad of dimensions surrounding this growing issue from our clinical practice. We present a wealth of recommendations in working through each of these dimensions through a multi-systems approach that include development of individualized care plan, access to consistent psychiatric services, and implementing short-term and long-term goals pertaining to treatment and placement. We also review our clinical practices that have supported these patients on a continuum at our healthcare institution which integrate our recommendations and also involve an open line of communication with established community partners involved in the care of these children. Furthermore, we propose suggestions from an operational perspective on developing a comprehensive, multidisciplinary care model for this fragile and oftentimes neglected patient population across the healthcare system as the basis to achieve equity and translational impact in the quality and delivery of healthcare care services across pediatric healthcare systems.
    Language English
    Publishing date 2023-01-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711999-3
    ISSN 2296-2360
    ISSN 2296-2360
    DOI 10.3389/fped.2022.1057956
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Synthesis, LC-MS/MS analysis, and biological evaluation of two vaccine candidates against ticks based on the antigenic P0 peptide from R. sanguineus linked to the p64K carrier protein from Neisseria meningitidis

    González, Luis Javier / Encinosa Guzmán, Pedro E. / Machado, Wendy / Pousa, Satomy / Leyva, Alejandro / Arguelles, Ana Laura Cano / Cabrera, Gleysin / Espinosa, Luis Ariel / Parra, Rubén / Hernández, Rachel / Soto, Yamil Bello / Ledesma, Frank L. / Joglar, Marisdania / Guirola, Osmany / Kurt, Louise Ulrich / Carvalho, Paulo C. / Cabrales, Ania / Garay, Hilda / Besada, Vladimir /
    Durán, Rosario / Takao, Toshifumi / Estrada, Mario Pablo / Rodríguez-Mallon, Alina

    Analytical and bioanalytical chemistry. 2021 Sept., v. 413, no. 23

    2021  

    Abstract: A peptide from the P0 acidic ribosomal protein (pP0) of ticks conjugated to keyhole limpet hemocyanin from Megathura crenulata has shown to be effective against different tick species when used in host vaccination. Turning this peptide into a commercial ... ...

    Abstract A peptide from the P0 acidic ribosomal protein (pP0) of ticks conjugated to keyhole limpet hemocyanin from Megathura crenulata has shown to be effective against different tick species when used in host vaccination. Turning this peptide into a commercial anti-tick vaccine will depend on finding the appropriate, technically and economically feasible way to present it to the host immune system. Two conjugates (p64K-Cys¹pP0 and p64K-βAla¹pP0) were synthesized using the p64K carrier protein from Neisseria meningitidis produced in Escherichia coli, the same cross-linking reagent, and two analogues of pP0. The SDS-PAGE analysis of p64K-Cys¹pP0 showed a heterogeneous conjugate compared to p64K-βAla¹pP0 that was detected as a protein band at 91kDa. The pP0/p64K ratio determined by MALDI-MS for p64K-Cys¹pP0 ranged from 1 to 8, being 3-5 the predominant ratio, while in the case of p64K-βAla¹pP0 this ratio was 5-7. Cys¹pP0 was partially linked to 35 out of 39 Lys residues and the N-terminal end, while βAla¹pP0 was mostly linked to the six free cysteine residues, to the N-terminal end, and, in a lesser extent, to Lys residues. The assignment of the conjugation sites and side reactions were based on the identification of type 2 peptides. Rabbit immunizations showed the best anti-pP0 titers and the highest efficacy against Rhipicephalus sanguineus ticks when the p64K-Cys¹pP0 was used as vaccine antigen. The presence of high molecular mass aggregates observed in the SDS-PAGE analysis of p64K-Cys¹pP0 could be responsible for a better immune response against pP0 and consequently for its better efficacy as an anti-tick vaccine. Graphical abstract
    Keywords Escherichia coli ; Megathura crenulata ; Neisseria meningitidis ; Rhipicephalus sanguineus ; analytical chemistry ; biological assessment ; cross-linking reagents ; cysteine ; economic feasibility ; immune response ; immune system ; keyhole limpet hemocyanin ; molecular weight ; peptides ; polyacrylamide gel electrophoresis ; ribosomal proteins ; ticks ; vaccination ; vaccines
    Language English
    Dates of publication 2021-09
    Size p. 5885-5900.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ISSN 1618-2642
    DOI 10.1007/s00216-021-03569-0
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Synthesis, LC-MS/MS analysis, and biological evaluation of two vaccine candidates against ticks based on the antigenic P0 peptide from R. sanguineus linked to the p64K carrier protein from Neisseria meningitidis.

    González, Luis Javier / Encinosa Guzmán, Pedro E / Machado, Wendy / Pousa, Satomy / Leyva, Alejandro / Arguelles, Ana Laura Cano / Cabrera, Gleysin / Espinosa, Luis Ariel / Parra, Rubén / Hernández, Rachel / Soto, Yamil Bello / Ledesma, Frank L / Joglar, Marisdania / Guirola, Osmany / Kurt, Louise Ulrich / Carvalho, Paulo C / Cabrales, Ania / Garay, Hilda / Besada, Vladimir /
    Durán, Rosario / Takao, Toshifumi / Estrada, Mario Pablo / Rodríguez-Mallon, Alina

    Analytical and bioanalytical chemistry

    2021  Volume 413, Issue 23, Page(s) 5885–5900

    Abstract: A peptide from the P0 acidic ribosomal protein (pP0) of ticks conjugated to keyhole limpet hemocyanin from Megathura crenulata has shown to be effective against different tick species when used in host vaccination. Turning this peptide into a commercial ... ...

    Abstract A peptide from the P0 acidic ribosomal protein (pP0) of ticks conjugated to keyhole limpet hemocyanin from Megathura crenulata has shown to be effective against different tick species when used in host vaccination. Turning this peptide into a commercial anti-tick vaccine will depend on finding the appropriate, technically and economically feasible way to present it to the host immune system. Two conjugates (p64K-Cys
    MeSH term(s) Animals ; Bacterial Outer Membrane Proteins/immunology ; Chromatography, Liquid/methods ; Electrophoresis, Polyacrylamide Gel ; Hemocyanins/immunology ; Neisseria meningitidis/immunology ; Rabbits ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Tandem Mass Spectrometry/methods ; Ticks/immunology ; Vaccines/immunology
    Chemical Substances Bacterial Outer Membrane Proteins ; Vaccines ; lpdA protein, Neisseria meningitidis ; Hemocyanins (9013-72-3) ; keyhole-limpet hemocyanin (FV4Y0JO2CX)
    Language English
    Publishing date 2021-08-03
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 201093-8
    ISSN 1618-2650 ; 0016-1152 ; 0372-7920
    ISSN (online) 1618-2650
    ISSN 0016-1152 ; 0372-7920
    DOI 10.1007/s00216-021-03569-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: BRCA1 transcriptionally regulates genes involved in breast tumorigenesis.

    Welcsh, Piri L / Lee, Ming K / Gonzalez-Hernandez, Rachel M / Black, Daniel J / Mahadevappa, Mamatha / Swisher, Elizabeth M / Warrington, Janet A / King, Mary-Claire

    Proceedings of the National Academy of Sciences of the United States of America

    2002  Volume 99, Issue 11, Page(s) 7560–7565

    Abstract: Loss of function of BRCA1 caused by inherited mutation and tissue-specific somatic mutation leads to breast and ovarian cancer. Nearly all BRCA1 germ-line mutations involve truncation or loss of the C-terminal BRCT transcriptional activation domain, ... ...

    Abstract Loss of function of BRCA1 caused by inherited mutation and tissue-specific somatic mutation leads to breast and ovarian cancer. Nearly all BRCA1 germ-line mutations involve truncation or loss of the C-terminal BRCT transcriptional activation domain, suggesting that transcriptional regulation is a critical function of the wild-type gene. The purpose of this project was to determine whether there is a link between the role of BRCA1 in transcriptional regulation and its role in tumor suppression. We developed a cell line (in which BRCA1 can be induced) and used microarray analysis to compare transcription profiles of epithelial cells with low endogenous levels of BRCA1 vs. transcription profiles of cells with 2-4-fold higher induced levels of expression of BRCA1. At these levels of expression, BRCA1 did not induce apoptosis. Undirected cluster analysis of six paired experiments revealed 373 genes, the expression of which was altered significantly and consistently by BRCA1 induction. Expression of 62 genes was altered more than 2-fold. BRCA1-regulated genes associated with breast tumorigenesis included the estrogen-responsive genes MYC and cyclin D1, which are overexpressed in many breast tumors; STAT1 and JAK1, key components of the cytokine signal transduction pathway; the extracellular matrix protein laminin 3A; ID4, an inhibitor of DNA-binding transcriptional activators, which in turn negatively regulates BRCA1 expression; and the prohormone stanniocalcin, expression of which is lost in breast tumor cells. Coordinated expression of BRCA1 with ID4 and with stanniocalcin was confirmed in primary breast and ovarian tumors.
    MeSH term(s) BRCA1 Protein/genetics ; Breast/cytology ; Breast/physiology ; Breast Neoplasms/genetics ; Cell Division/genetics ; Cell Line ; Cell Transformation, Neoplastic/genetics ; Cloning, Molecular ; DNA, Complementary/genetics ; Ecdysterone/analogs & derivatives ; Ecdysterone/pharmacology ; Epithelial Cells/cytology ; Epithelial Cells/physiology ; Female ; Gene Expression Regulation/drug effects ; Gene Expression Regulation, Neoplastic ; Genes, BRCA1 ; Humans ; Kidney ; Kinetics ; Mutation ; Ovarian Neoplasms/genetics ; Transfection
    Chemical Substances BRCA1 Protein ; DNA, Complementary ; Ecdysterone (5289-74-7) ; ponasterone A (84986BG3NG)
    Language English
    Publishing date 2002-05-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.062181799
    Database MEDical Literature Analysis and Retrieval System OnLINE

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