LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 34

Search options

  1. Article ; Online: Wrap to sort.

    Hernández-Ainsa, Silvia

    Nature chemistry

    2021  Volume 13, Issue 4, Page(s) 301–302

    MeSH term(s) Centrifugation ; DNA ; Liposomes
    Chemical Substances Liposomes ; DNA (9007-49-2)
    Language English
    Publishing date 2021-03-30
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 2464596-5
    ISSN 1755-4349 ; 1755-4330
    ISSN (online) 1755-4349
    ISSN 1755-4330
    DOI 10.1038/s41557-021-00673-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Assessing the influence of small structural modifications in simple DNA-based nanostructures on their role as drug nanocarriers.

    Postigo, Alejandro / Martínez-Vicente, Pablo / Baumann, Kevin N / Del Barrio, Jesús / Hernández-Ainsa, Silvia

    Biomaterials science

    2024  Volume 12, Issue 6, Page(s) 1549–1557

    Abstract: DNA nanotechnology leverages Watson-Crick-Franklin base-pairing interactions to build complex DNA-based nanostructures (DNS). Due to DNA specific self-assembly properties, DNS can be designed with a total control of their architecture, which has been ... ...

    Abstract DNA nanotechnology leverages Watson-Crick-Franklin base-pairing interactions to build complex DNA-based nanostructures (DNS). Due to DNA specific self-assembly properties, DNS can be designed with a total control of their architecture, which has been demonstrated to have an impact on the overall DNS features. Indeed, structural properties such as the shape, size and flexibility of DNS can influence their biostability as well as their ability to internalise into cells. We present here two series of simple DNS with small and precise variations related to their length or flexibility and study the influence that these structural changes have on their overall properties as drug nanocarriers. Results indicate that shorter and more flexible DNS present higher stability towards nuclease degradation. These structural changes also have a certain effect on their cell internalisation ability and drug release rate. Consequently, drug-loaded DNS cytotoxicity varies according to the design, with lower cell viability values obtained in the DNS exhibiting faster drug release and larger cell interaction rates. In summary, small changes in the structure of simple DNS can have an influence on their overall capabilities as drug nanocarriers. The effects reported here could guide the design of simple DNS for future therapeutic uses.
    MeSH term(s) Nanostructures/chemistry ; DNA/chemistry ; Nanotechnology/methods ; Cell Survival
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2024-03-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2693928-9
    ISSN 2047-4849 ; 2047-4830
    ISSN (online) 2047-4849
    ISSN 2047-4830
    DOI 10.1039/d3bm01987j
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Dendron-functionalised hyperbranched bis-MPA polyesters as efficient non-viral vectors for gene therapy in different cell lines.

    San Anselmo, María / Postigo, Alejandro / Lancelot, Alexandre / Serrano, José Luis / Sierra, Teresa / Hernández-Ainsa, Silvia

    Biomaterials science

    2022  Volume 10, Issue 10, Page(s) 2706–2719

    Abstract: Gene therapy has become a relevant tool in the biomedical field to treat or even prevent some diseases. The effective delivery of genetic material into the cell remains a crucial step to succeed in this purpose. In the search for efficient non-viral ... ...

    Abstract Gene therapy has become a relevant tool in the biomedical field to treat or even prevent some diseases. The effective delivery of genetic material into the cell remains a crucial step to succeed in this purpose. In the search for efficient non-viral vectors, a series of amino-terminated dendronized hyperbranched polymers (DHPs) of different generations based either on bis-MPA or bis-GMPA have been designed. All of them have demonstrated an accurate ability to complex two types of genetic materials, a plasmid DNA and a siGFP, yielding dendriplexes. Moreover, some of them have proved to be able to deliver the genetic material inside the cells, resulting in the effective accomplishment of the desired genetic modification and improving the activity of some commercial transfection reagents. Different cell lines, including cancer and mesenchymal stem cells, have been studied here to evaluate the ability of DHPs as vectors for transfection. Treatments based on mesenchymal stem cells are gaining importance due to their pluripotency. Thus, it is of special relevance to introduce a genetic modification into a mesenchymal cell line as it allows it to act over a wide spectrum of tissues after inducing cellular differentiation.
    MeSH term(s) Cell Line ; Dendrimers ; Gene Transfer Techniques ; Genetic Therapy ; Genetic Vectors/genetics ; Polyesters ; Polymers ; Transfection
    Chemical Substances Dendrimers ; Polyesters ; Polymers
    Language English
    Publishing date 2022-05-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 2693928-9
    ISSN 2047-4849 ; 2047-4830
    ISSN (online) 2047-4849
    ISSN 2047-4830
    DOI 10.1039/d2bm00365a
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: DNA-Liposome Hybrid Carriers for Triggered Cargo Release.

    Baumann, Kevin N / Schröder, Tim / Ciryam, Prashanth S / Morzy, Diana / Tinnefeld, Philip / Knowles, Tuomas P J / Hernández-Ainsa, Silvia

    ACS applied bio materials

    2022  Volume 5, Issue 8, Page(s) 3713–3721

    Abstract: The design of simple and versatile synthetic routes to accomplish triggered-release properties in carriers is of particular interest for drug delivery purposes. In this context, the programmability and adaptability of DNA nanoarchitectures in combination ...

    Abstract The design of simple and versatile synthetic routes to accomplish triggered-release properties in carriers is of particular interest for drug delivery purposes. In this context, the programmability and adaptability of DNA nanoarchitectures in combination with liposomes have great potential to render biocompatible hybrid carriers for triggered cargo release. We present an approach to form a DNA mesh on large unilamellar liposomes incorporating a stimuli-responsive DNA building block. Upon incubation with a single-stranded DNA trigger sequence, a hairpin closes, and the DNA building block is allowed to self-contract. We demonstrate the actuation of this building block by single-molecule Förster resonance energy transfer (FRET), fluorescence recovery after photobleaching, and fluorescence quenching measurements. By triggering this process, we demonstrate the elevated release of the dye calcein from the DNA-liposome hybrid carriers. Interestingly, the incubation of the doxorubicin-laden active hybrid carrier with HEK293T cells suggests increased cytotoxicity relative to a control carrier without the triggered-release mechanism. In the future, the trigger could be provided by peritumoral nucleic acid sequences and lead to site-selective release of encapsulated chemotherapeutics.
    MeSH term(s) DNA ; Doxorubicin ; Drug Delivery Systems ; HEK293 Cells ; Humans ; Liposomes
    Chemical Substances Liposomes ; Doxorubicin (80168379AG) ; DNA (9007-49-2)
    Language English
    Publishing date 2022-07-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2576-6422
    ISSN (online) 2576-6422
    DOI 10.1021/acsabm.2c00225
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: DNA-Based Nanocarriers to Enhance the Optoacoustic Contrast of Tumors In Vivo.

    Joseph, James / Baumann, Kevin N / Postigo, Alejandro / Bollepalli, Laura / Bohndiek, Sarah E / Hernández-Ainsa, Silvia

    Advanced healthcare materials

    2020  Volume 10, Issue 2, Page(s) e2001739

    Abstract: Optoacoustic tomography (OT) enables non-invasive deep tissue imaging of optical contrast at high spatio-temporal resolution. The applications of OT in cancer imaging often rely on the use of molecular imaging contrast agents based on near-infrared (NIR) ...

    Abstract Optoacoustic tomography (OT) enables non-invasive deep tissue imaging of optical contrast at high spatio-temporal resolution. The applications of OT in cancer imaging often rely on the use of molecular imaging contrast agents based on near-infrared (NIR) dyes to enhance contrast at the tumor site. While these agents afford excellent biocompatibility and minimal toxicity, they present limited optoacoustic signal generation capability and rapid renal clearance, which can impede their tumor imaging efficacy. In this work, a synthetic strategy to overcome these limitations utilizing biodegradable DNA-based nanocarrier (DNA-NC) platforms is introduced. DNA-NCs enable the incorporation of NIR dyes (in this case, IRDye 800CW) at precise positions to enable fluorescence quenching and maximize optoacoustic signal generation. Furthermore, these DNA-NCs show a prolonged blood circulation compared to the native fluorophores, facilitating tumor accumulation by the enhanced permeability and retention (EPR) effect. In vivo imaging of tumor xenografts in mice following intravenous administration of DNA-NCs reveals enhanced OT signals at 24 h when compared to free fluorophores, indicating promise for this method to enhance the optoacoustic signal generation capability and tumor uptake of clinically relevant NIR dyes.
    MeSH term(s) Animals ; DNA ; Fluorescent Dyes ; Mice ; Molecular Imaging ; Neoplasms/diagnostic imaging
    Chemical Substances Fluorescent Dyes ; DNA (9007-49-2)
    Language English
    Publishing date 2020-11-16
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2649576-4
    ISSN 2192-2659 ; 2192-2640
    ISSN (online) 2192-2659
    ISSN 2192-2640
    DOI 10.1002/adhm.202001739
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6966: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: An active DNA-based nanoprobe for photoacoustic pH imaging

    Baumann, Kevin N. / Fux, Alexandra C. / Joseph, James / Bohndiek, Sarah E. / Hernández-Ainsa, Silvia

    Chemical communications. 2018 Sept. 6, v. 54, no. 72 p.10176-10178

    2018  

    Abstract: We report an active DNA construct capable of probing pH through a photoacoustic (PA) ratiometric analysis approach. Our nanoprobe enables different PA readout in tissue mimicking phantoms in the range between pH 6.8 to 7.8 at physiologically relevant ... ...

    Abstract We report an active DNA construct capable of probing pH through a photoacoustic (PA) ratiometric analysis approach. Our nanoprobe enables different PA readout in tissue mimicking phantoms in the range between pH 6.8 to 7.8 at physiologically relevant sodium concentrations. Thus, it represents a promising platform to probe pH values relevant to the tumor microenvironment using PA.
    Keywords DNA ; chemical reactions ; image analysis ; neoplasms ; pH ; sodium
    Language English
    Dates of publication 2018-0906
    Size p. 10176-10178.
    Publishing place The Royal Society of Chemistry
    Document type Article ; Online
    Note Resource is Open Access
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/c8cc04007a
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Heparin-Coated Dendronized Hyperbranched Polymers for Antimalarial Targeted Delivery.

    San Anselmo, María / Lantero, Elena / Avalos-Padilla, Yunuen / Bouzón-Arnáiz, Inés / Ramírez, Miriam / Postigo, Alejandro / Serrano, José Luis / Sierra, Teresa / Hernández-Ainsa, Silvia / Fernàndez-Busquets, Xavier

    ACS applied polymer materials

    2022  Volume 5, Issue 1, Page(s) 381–390

    Abstract: The rampant evolution of resistance ... ...

    Abstract The rampant evolution of resistance in
    Language English
    Publishing date 2022-12-30
    Publishing country United States
    Document type Journal Article
    ISSN 2637-6105
    ISSN (online) 2637-6105
    DOI 10.1021/acsapm.2c01553
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article ; Online: Micellar Nanocarriers from Dendritic Macromolecules Containing Fluorescent Coumarin Moieties.

    Concellón, Alberto / San Anselmo, María / Hernández-Ainsa, Silvia / Romero, Pilar / Marcos, Mercedes / Serrano, José Luis

    Polymers

    2020  Volume 12, Issue 12

    Abstract: The design of efficient drug-delivery vehicles remains a big challenge in materials science. Herein, we describe a novel class of amphiphilic hybrid dendrimers that consist of a poly(amidoamine) (PAMAM) dendritic core functionalized ... ...

    Abstract The design of efficient drug-delivery vehicles remains a big challenge in materials science. Herein, we describe a novel class of amphiphilic hybrid dendrimers that consist of a poly(amidoamine) (PAMAM) dendritic core functionalized with
    Language English
    Publishing date 2020-11-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym12122872
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: Coating and Stabilization of Liposomes by Clathrin-Inspired DNA Self-Assembly.

    Baumann, Kevin N / Piantanida, Luca / García-Nafría, Javier / Sobota, Diana / Voïtchovsky, Kislon / Knowles, Tuomas P J / Hernández-Ainsa, Silvia

    ACS nano

    2020  Volume 14, Issue 2, Page(s) 2316–2323

    Abstract: The self-assembly of the protein clathrin on biological membranes facilitates essential processes of endocytosis and has provided a source of inspiration for materials design by the highly ordered structural appearance. By mimicking the architecture of ... ...

    Abstract The self-assembly of the protein clathrin on biological membranes facilitates essential processes of endocytosis and has provided a source of inspiration for materials design by the highly ordered structural appearance. By mimicking the architecture of the protein building blocks and clathrin self-assemblies to coat liposomes with biomaterials, advanced hybrid carriers can be derived. Here, we present a method for fabricating DNA-coated liposomes by hydrophobically anchoring and subsequently connecting DNA-based triskelion structures on the liposome surface inspired by the assembly of the protein clathrin. Dynamic light scattering, ζ-potential, confocal microscopy, and cryo-electron microscopy measurements independently demonstrate successful DNA coating. Nanomechanical measurements conducted with atomic force microscopy show that the DNA coating enhances the mechanical stability of the liposomes relative to uncoated ones. Furthermore, we provide the possibility to reverse the coating process by triggering the disassembly of the DNA coats through a toehold-mediated displacement reaction. Our results describe a straightforward, versatile, and reversible approach for coating and stabilizing lipid vesicles through the assembly of rationally designed DNA structures. This method has potential for further development toward the ordered arrangement of tailored functionalities on the surface of liposomes and for applications as hybrid nanocarriers.
    MeSH term(s) Clathrin/chemistry ; DNA/chemical synthesis ; DNA/chemistry ; Hydrophobic and Hydrophilic Interactions ; Liposomes/chemistry ; Particle Size ; Surface Properties
    Chemical Substances Clathrin ; Liposomes ; DNA (9007-49-2)
    Language English
    Publishing date 2020-02-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1936-086X
    ISSN (online) 1936-086X
    DOI 10.1021/acsnano.9b09453
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article ; Online: Proton conductive ionic liquid crystalline poly(ethyleneimine) polymers functionalized with oxadiazole.

    Concellón, Alberto / Hernández-Ainsa, Silvia / Barberá, Joaquín / Romero, Pilar / Serrano, José Luis / Marcos, Mercedes

    RSC advances

    2018  Volume 8, Issue 66, Page(s) 37700–37706

    Abstract: Two novel series of ionic liquid crystal polymers that display proton conductive properties are presented here. These materials are based on linear ( ...

    Abstract Two novel series of ionic liquid crystal polymers that display proton conductive properties are presented here. These materials are based on linear (
    Language English
    Publishing date 2018-11-08
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/c8ra08253g
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top