Article ; Online: Cytokine and T cell responses in post-chikungunya viral arthritis: A cross-sectional study.
2024 Volume 19, Issue 3, Page(s) e0299521
Abstract: Objective: To define the relationship between chronic chikungunya post-viral arthritis disease severity, cytokine response and T cell subsets in order to identify potential targets for therapy.: Methods: Participants with chikungunya arthritis were ... ...
| Abstract | Objective: To define the relationship between chronic chikungunya post-viral arthritis disease severity, cytokine response and T cell subsets in order to identify potential targets for therapy. Methods: Participants with chikungunya arthritis were recruited from Colombia from 2019-2021. Arthritis disease severity was quantified using the Disease Activity Score-28 and an Arthritis-Flare Questionnaire adapted for chikungunya arthritis. Plasma cytokine concentrations (interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, interferon-γ and tumor necrosis factor (TNF)) were measured using a Meso Scale Diagnostics assay. Peripheral blood T cell subsets were measured using flow cytometry. Results: Among participants with chikungunya arthritis (N = 158), IL-2 levels and frequency of regulatory T cells (Tregs) were low. Increased arthritis disease activity was associated with higher levels of inflammatory cytokines (IL-6, TNF and CRP) and immunoregulatory cytokine IL-10 (p<0.05). Increased arthritis flare activity was associated with higher Treg frequencies (p<0.05) without affecting T effector (Teff) frequencies, Treg/Teff ratios and Treg subsets. Finally, elevated levels of IL-2 were correlated with increased Treg frequency, percent Tregs out of CD4+ T cells, and Treg subsets expressing immunosuppressive markers, while also correlating with an increased percent Teff out of live lymphocytes (p<0.05). Conclusion: Chikungunya arthritis is characterized by increased inflammatory cytokines and deficient IL-2 and Treg responses. Greater levels of IL-2 were associated with improved Treg numbers and immunosuppressive markers. Future research may consider targeting these pathways for therapy. |
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| MeSH term(s) | Humans ; Cytokines/metabolism ; Interleukin-10/metabolism ; Cross-Sectional Studies ; Interleukin-2/metabolism ; Interleukin-6/metabolism ; Chikungunya Fever/complications ; T-Lymphocytes, Regulatory/metabolism ; CD4-Positive T-Lymphocytes/metabolism ; Tumor Necrosis Factor-alpha/metabolism ; Arthritis, Infectious ; Immunosuppressive Agents |
| Chemical Substances | Cytokines ; Interleukin-10 (130068-27-8) ; Interleukin-2 ; Interleukin-6 ; Tumor Necrosis Factor-alpha ; Immunosuppressive Agents |
| Language | English |
| Publishing date | 2024-03-20 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2267670-3 |
| ISSN | 1932-6203 ; 1932-6203 |
| ISSN (online) | 1932-6203 |
| ISSN | 1932-6203 |
| DOI | 10.1371/journal.pone.0299521 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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