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  1. Book ; Thesis: Gesundheitsbezogene Lebensqualität bei Patienten mit Vorhofflimmern

    Herwig, Susanne

    eine kontrollierte Multizenterstudie

    2002  

    Author's details vorgelegt von: Susanne Herwig
    Language German
    Size 101 S. : graph. Darst.
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Bonn, Univ., Diss., 2001
    HBZ-ID HT013234036
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2002 D 935 order for loan Magazin

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  2. Article: Improving the Antigenicity of SARS-CoV-2 Vaccine Genes by Merging Mutations from Different Variants of Concern.

    Herwig, Susanne / Adler, Julia M / Vladimirova, Daria / Trimpert, Jakob / Sehouli, Jalid / Cichon, Günter

    Vaccines

    2024  Volume 12, Issue 3

    Abstract: During the COVID-19 pandemic, the early emergence of viral variants repeatedly undermined the effects of vaccination. Our aim here is to explore strategies for improving spike vaccine gene antigenicity by merging mutations from different variants of ... ...

    Abstract During the COVID-19 pandemic, the early emergence of viral variants repeatedly undermined the effects of vaccination. Our aim here is to explore strategies for improving spike vaccine gene antigenicity by merging mutations from different variants of concern (VOCs) in a single vaccine gene. To this end, newly developed recombinant vaccine genes were designed, cloned into adenoviral vectors, and applied to C57BL/6 mice; then, serum-neutralizing antibodies against the wildtype SARS-CoV-2 strains were determined in neutralization assays. The merger of mutations from different variants of concern (alpha, beta, gamma, and delta) in a single recombinant spike-based vaccine gene provided a substantial improvement in neutralizing immunity to all variants of concern, including the omicron strains. To date, only unmodified spike genes of the original SARS-CoV-2 Wuhan strain (B.1) or dominant variants (BA.1, BA.5, and XBB.1.5) have been used as vaccine genes. The employment of unmodified vaccine genes is afflicted by limited cross-protection among variant strains. In contrast, recombinant vaccine genes that combine mutations from different strains in a single gene hold the potential to broaden and improve immune protection and might help to reduce the need for frequent vaccine adaptations in the future.
    Language English
    Publishing date 2024-02-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines12030248
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines-A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters.

    Trimpert, Jakob / Herwig, Susanne / Stein, Julia / Vladimirova, Daria / Adler, Julia M / Abdelgawad, Azza / Firsching, Theresa C / Thoma, Tizia / Sehouli, Jalid / Osterrieder, Klaus / Gruber, Achim D / Sawitzki, Birgit / Sander, Leif Erik / Cichon, Günter

    Viruses

    2021  Volume 13, Issue 11

    Abstract: With the exception of inactivated vaccines, all SARS-CoV-2 vaccines currently used for clinical application focus on the spike envelope glycoprotein as a virus-specific antigen. Compared to other SARS-CoV-2 genes, mutations in the spike protein gene are ... ...

    Abstract With the exception of inactivated vaccines, all SARS-CoV-2 vaccines currently used for clinical application focus on the spike envelope glycoprotein as a virus-specific antigen. Compared to other SARS-CoV-2 genes, mutations in the spike protein gene are more rapidly selected and spread within the population, which carries the risk of impairing the efficacy of spike-based vaccines. It is unclear to what extent the loss of neutralizing antibody epitopes can be compensated by cellular immune responses, and whether the use of other SARS-CoV-2 antigens might cause a more diverse immune response and better long-term protection, particularly in light of the continued evolution towards new SARS-CoV-2 variants. To address this question, we explored immunogenicity and protective effects of adenoviral vectors encoding either the full-length spike protein (S), the nucleocapsid protein (N), the receptor binding domain (RBD) or a hybrid construct of RBD and the membrane protein (M) in a highly susceptible COVID-19 hamster model. All adenoviral vaccines provided life-saving protection against SARS-CoV-2-infection. The most efficient protection was achieved after exposure to full-length spike. However, the nucleocapsid protein, which triggered a robust T-cell response but did not facilitate the formation of neutralizing antibodies, controlled early virus replication efficiently and prevented severe pneumonia. Although the full-length spike protein is an excellent target for vaccines, it does not appear to be the only option for future vaccine design.
    MeSH term(s) Animals ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; Antigens, Viral/immunology ; CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; COVID-19/immunology ; COVID-19/pathology ; COVID-19/prevention & control ; COVID-19/virology ; COVID-19 Vaccines/immunology ; Coronavirus Nucleocapsid Proteins/genetics ; Coronavirus Nucleocapsid Proteins/immunology ; Cricetinae ; Female ; Immunity, Cellular ; Immunity, Humoral ; Immunogenicity, Vaccine ; Inflammation ; Lung/pathology ; Lung/virology ; Male ; Mice, Inbred C57BL ; Phosphoproteins/genetics ; Phosphoproteins/immunology ; SARS-CoV-2/immunology ; SARS-CoV-2/physiology ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/immunology ; Viral Matrix Proteins/genetics ; Viral Matrix Proteins/immunology ; Mice
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Antigens, Viral ; COVID-19 Vaccines ; Coronavirus Nucleocapsid Proteins ; Phosphoproteins ; Spike Glycoprotein, Coronavirus ; Viral Matrix Proteins ; membrane protein, SARS-CoV-2 ; nucleocapsid phosphoprotein, SARS-CoV-2 ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2021-11-16
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13112290
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Deciphering the Role of Humoral and Cellular Immune Responses in Different COVID-19 Vaccines—A Comparison of Vaccine Candidate Genes in Roborovski Dwarf Hamsters

    Trimpert, Jakob / Herwig, Susanne / Stein, Julia / Vladimirova, Daria / Adler, Julia M. / Abdelgawad, Azza / Firsching, Theresa C. / Thoma, Tizia / Sehouli, Jalid / Osterrieder, Klaus / Gruber, Achim D. / Sawitzki, Birgit / Sander, Leif Erik / Cichon, Günter

    Viruses. 2021 Nov. 16, v. 13, no. 11

    2021  

    Abstract: With the exception of inactivated vaccines, all SARS-CoV-2 vaccines currently used for clinical application focus on the spike envelope glycoprotein as a virus-specific antigen. Compared to other SARS-CoV-2 genes, mutations in the spike protein gene are ... ...

    Abstract With the exception of inactivated vaccines, all SARS-CoV-2 vaccines currently used for clinical application focus on the spike envelope glycoprotein as a virus-specific antigen. Compared to other SARS-CoV-2 genes, mutations in the spike protein gene are more rapidly selected and spread within the population, which carries the risk of impairing the efficacy of spike-based vaccines. It is unclear to what extent the loss of neutralizing antibody epitopes can be compensated by cellular immune responses, and whether the use of other SARS-CoV-2 antigens might cause a more diverse immune response and better long-term protection, particularly in light of the continued evolution towards new SARS-CoV-2 variants. To address this question, we explored immunogenicity and protective effects of adenoviral vectors encoding either the full-length spike protein (S), the nucleocapsid protein (N), the receptor binding domain (RBD) or a hybrid construct of RBD and the membrane protein (M) in a highly susceptible COVID-19 hamster model. All adenoviral vaccines provided life-saving protection against SARS-CoV-2-infection. The most efficient protection was achieved after exposure to full-length spike. However, the nucleocapsid protein, which triggered a robust T-cell response but did not facilitate the formation of neutralizing antibodies, controlled early virus replication efficiently and prevented severe pneumonia. Although the full-length spike protein is an excellent target for vaccines, it does not appear to be the only option for future vaccine design.
    Keywords Adenoviridae ; COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; T-lymphocytes ; animal models ; epitopes ; evolution ; genes ; glycoproteins ; hybrids ; immune response ; immunogenicity ; membrane proteins ; nucleocapsid proteins ; pneumonia ; risk ; vaccine development ; virus replication
    Language English
    Dates of publication 2021-1116
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13112290
    Database NAL-Catalogue (AGRICOLA)

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  5. Book ; Thesis: Gesundheitsbezogene Lebensqualität bei Patienten mit Vorhofflimmern

    Herwig, Susanne

    eine kontrollierte Multizenterstudie

    2002  

    Author's details vorgelegt von: Susanne Herwig
    Language German
    Size 101 S, graph. Darst, 21 cm
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., Diss--Bonn, 2001
    Database Former special subject collection: coastal and deep sea fishing

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  6. Article ; Online: Live-attenuated vaccine sCPD9 elicits superior mucosal and systemic immunity to SARS-CoV-2 variants in hamsters.

    Nouailles, Geraldine / Adler, Julia M / Pennitz, Peter / Peidli, Stefan / Teixeira Alves, Luiz Gustavo / Baumgardt, Morris / Bushe, Judith / Voss, Anne / Langenhagen, Alina / Langner, Christine / Martin Vidal, Ricardo / Pott, Fabian / Kazmierski, Julia / Ebenig, Aileen / Lange, Mona V / Mühlebach, Michael D / Goekeri, Cengiz / Simmons, Szandor / Xing, Na /
    Abdelgawad, Azza / Herwig, Susanne / Cichon, Günter / Niemeyer, Daniela / Drosten, Christian / Goffinet, Christine / Landthaler, Markus / Blüthgen, Nils / Wu, Haibo / Witzenrath, Martin / Gruber, Achim D / Praktiknjo, Samantha D / Osterrieder, Nikolaus / Wyler, Emanuel / Kunec, Dusan / Trimpert, Jakob

    Nature microbiology

    2023  Volume 8, Issue 5, Page(s) 860–874

    Abstract: Vaccines play a critical role in combating the COVID-19 pandemic. Future control of the pandemic requires improved vaccines with high efficacy against newly emerging SARS-CoV-2 variants and the ability to reduce virus transmission. Here we compare immune ...

    Abstract Vaccines play a critical role in combating the COVID-19 pandemic. Future control of the pandemic requires improved vaccines with high efficacy against newly emerging SARS-CoV-2 variants and the ability to reduce virus transmission. Here we compare immune responses and preclinical efficacy of the mRNA vaccine BNT162b2, the adenovirus-vectored spike vaccine Ad2-spike and the live-attenuated virus vaccine candidate sCPD9 in Syrian hamsters, using both homogeneous and heterologous vaccination regimens. Comparative vaccine efficacy was assessed by employing readouts from virus titrations to single-cell RNA sequencing. Our results show that sCPD9 vaccination elicited the most robust immunity, including rapid viral clearance, reduced tissue damage, fast differentiation of pre-plasmablasts, strong systemic and mucosal humoral responses, and rapid recall of memory T cells from lung tissue after challenge with heterologous SARS-CoV-2. Overall, our results demonstrate that live-attenuated vaccines offer advantages over currently available COVID-19 vaccines.
    MeSH term(s) Animals ; Cricetinae ; Humans ; Vaccines, Attenuated ; SARS-CoV-2 ; COVID-19/prevention & control ; COVID-19 Vaccines ; BNT162 Vaccine ; Pandemics ; Mesocricetus
    Chemical Substances sCPD9 COVID-19 vaccine ; Vaccines, Attenuated ; COVID-19 Vaccines ; BNT162 Vaccine
    Language English
    Publishing date 2023-04-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2058-5276
    ISSN (online) 2058-5276
    DOI 10.1038/s41564-023-01352-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

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  7. Book ; Thesis: Quervernetzungsstudien an 50S-Untereinheiten von Bacillus stearothermophilus und Klonierung im S10-Operon

    Herwig, Susanne

    1991  

    Language Undetermined
    Size 2 Mikrofiches, 25x
    Document type Book ; Thesis
    Thesis / German Habilitation thesis @Berlin, Freie Univ., Diss. : 1991 (Nur als Mikrofiche für den Austausch)
    Note Mikroreprod. eines Ms. Getr. Zählung : Ill., graph. Darst
    Database Former special subject collection: coastal and deep sea fishing

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    Inter-library loan at ZB MED

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  8. Conference proceedings: Zi-Kodierhilfe – ein praxistaugliches Werkzeug zur Verbesserung der Kodierqualität

    Jelastopulu, Eleni / Herwig, Susanne / Martini, Ina / Fischer, Ina / Gillwaldt, Nicole / Bartkowski, Rolf / Engelhardt, Rita / von Stillfried, Dominik

    2015  , Page(s) P100

    Event/congress 14. Deutscher Kongress für Versorgungsforschung; Berlin; Deutsches Netzwerk Versorgungsforschung; 2015
    Keywords Medizin, Gesundheit
    Publishing date 2015-09-22
    Publisher German Medical Science GMS Publishing House; Düsseldorf
    Document type Conference proceedings
    DOI 10.3205/15dkvf183
    Database German Medical Science

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  9. Article: Delayed-late activation of a myeloid defensin minimal promoter by retinoids and inflammatory mediators.

    Wang, Nan / Su, Qin / Boeckh-Herwig, Susanne / Yaneva, Mariana / Tempst, Paul

    Leukemia research

    2004  Volume 28, Issue 8, Page(s) 879–889

    Abstract: Alpha-defensin-1 gene expression in promyelocytic HL-60 cells is ('delayed-late' > or =1-2 days) activated by retinoic acid (RA), lipopolysaccharide, tumor necrosis factor-alpha and elevated levels of cAMP. Using stably integrated reporter constructs, we ...

    Abstract Alpha-defensin-1 gene expression in promyelocytic HL-60 cells is ('delayed-late' > or =1-2 days) activated by retinoic acid (RA), lipopolysaccharide, tumor necrosis factor-alpha and elevated levels of cAMP. Using stably integrated reporter constructs, we show that this activation is directed through a proximal and distal element within a minimal (-83/+82) def1 promoter, and is mediated by phosphorylation of the associated factors, PU.1 and D1BP, in an inducer-dependent manner. Whereas binding of PU.1 to the proximal element confers cell specificity and relays the effects of most inducers, the selectively enhancing capacity of the distal element for RA- and cAMP-dependent activation is uniquely correlated with D1BP-binding. We propose that D1BP and PU.1 are the end-points of separate pathways.
    MeSH term(s) Antineoplastic Agents/pharmacology ; Binding Sites ; Cyclic AMP/pharmacology ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Neoplastic/drug effects ; Genes, Reporter ; HL-60 Cells ; HeLa Cells ; Humans ; Lipopolysaccharides/pharmacology ; Phosphorylation/drug effects ; Promoter Regions, Genetic ; Proto-Oncogene Proteins/metabolism ; Regulatory Sequences, Nucleic Acid ; Retinoids/pharmacology ; Sequence Deletion ; Trans-Activators/metabolism ; Transcription, Genetic/drug effects ; Tumor Necrosis Factor-alpha/pharmacology ; alpha-Defensins/genetics ; alpha-Defensins/metabolism
    Chemical Substances Antineoplastic Agents ; Lipopolysaccharides ; Proto-Oncogene Proteins ; Retinoids ; Trans-Activators ; Tumor Necrosis Factor-alpha ; alpha-Defensins ; proto-oncogene protein Spi-1 ; Cyclic AMP (E0399OZS9N)
    Language English
    Publishing date 2004-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 752396-8
    ISSN 1873-5835 ; 0145-2126
    ISSN (online) 1873-5835
    ISSN 0145-2126
    DOI 10.1016/j.leukres.2003.12.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1321: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article: Hepatocarcinoma cells constitutively expressing adenoviral E1-genes provide a tumor model for intratumoral replication of E1-deficient adenoviruses.

    Cichon, Guenter / Boeckh-Herwig, Susanne / Elezkurtaj, Sefer / Schmidt, Hartmut H / Hofmann, Christian / Arnold, Wolfgang

    Anticancer research

    2002  Volume 22, Issue 1A, Page(s) 197–201

    Abstract: The elimination of malignant tumors by intratumoral virus replication is a challenging therapeutic approach but is critically dependent on the speed and efficacy of intratumoral virus spread. The expression of oncolytic transgenes in the context of a ... ...

    Abstract The elimination of malignant tumors by intratumoral virus replication is a challenging therapeutic approach but is critically dependent on the speed and efficacy of intratumoral virus spread. The expression of oncolytic transgenes in the context of a replicating virus may help to enhance the therapeutic potency of this strategy. We have established a human hepatocarcinoma-derived cell line (Huh7-E1) which stably expresses adenoviral E1-genes. Tumors derived from these cells support replication of E1-deficient adenoviruses in SCID mice. This model can be used to evaluate E1-negative viruses encoding reporter genes or oncolytic transgenes in a replicating context. Most oncolytic viruses for human use could then be re-engineered as E1-postive viruses. Moreover, Huh7-E1 tumors release human alpha-1-antitrypsin (hAAT), which allows the monitoring of occult growing tumors (i.e. liver, peritoneum) by measuring serum hAAT levels.
    MeSH term(s) Adenoviridae/genetics ; Adenoviridae/physiology ; Adenovirus E1 Proteins/biosynthesis ; Adenovirus E1 Proteins/genetics ; Animals ; Carcinoma, Hepatocellular/genetics ; Carcinoma, Hepatocellular/metabolism ; Carcinoma, Hepatocellular/therapy ; Carcinoma, Hepatocellular/virology ; Cell Division/physiology ; Genes, Viral ; Humans ; Liver Neoplasms/genetics ; Liver Neoplasms/metabolism ; Liver Neoplasms/therapy ; Liver Neoplasms/virology ; Mice ; Mice, SCID ; Tumor Cells, Cultured ; Virus Replication ; Xenograft Model Antitumor Assays ; alpha 1-Antitrypsin/metabolism
    Chemical Substances Adenovirus E1 Proteins ; alpha 1-Antitrypsin
    Language English
    Publishing date 2002-01
    Publishing country Greece
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604549-2
    ISSN 1791-7530 ; 0250-7005
    ISSN (online) 1791-7530
    ISSN 0250-7005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1642: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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