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Article ; Online: Bicellular Localization of Tricellular Junctional Protein Angulin-3/ILDR2 Allows Detection of Podocyte Injury.

Higashi, Atsuko Y / Saito, Akira C / Higashi, Tomohito / Furuse, Kyoko / Furuse, Mikio / Chiba, Hideki / Kazama, Junichiro J

The American journal of pathology

2024 Volume 194, Issue 5, Page(s) 673–683

Abstract: Podocytes serve as part of the renal filtration unit with slit diaphragms. Although the structure of slit diaphragms between two cells is well characterized, how the tricellular contact of podocytes is organized and how it changes in injured podocytes ... ...

Abstract	Podocytes serve as part of the renal filtration unit with slit diaphragms. Although the structure of slit diaphragms between two cells is well characterized, how the tricellular contact of podocytes is organized and how it changes in injured podocytes remains unknown. This study focused on a tricellular junction protein, angulin-3, and its localization in healthy podocytes, in developmental stages, and in pathologic conditions, using a newly established monoclonal antibody. Angulin-3 was confined at tricellular junctions of primordial podocytes, then transiently localized at bicellular junctions as foot process interdigitation developed and the intercellular junctions rearranged into slit diaphragm, and eventually distributed in a sparse punctate pattern on the foot processes of adult podocytes. In the rodent podocyte injury models, angulin-3 showed bicellular localization between the foot processes, and the localization turned from punctate to dashed linear pattern along the effaced foot processes with the progression of podocyte injury. Angulin-3 also accumulated between foot processes in a linear pattern in kidney biopsy samples of human nephrotic syndrome. Additionally, the line length of angulin-3 staining signal correlated with risk of relapse under glucocorticoid therapy in patients with minimal change nephrotic syndrome. This study proposes an image program to score the linearity of the accumulation pattern of angulin-3 to evaluate the relapse risk of patients with minimal change nephrotic syndrome.
MeSH term(s)	Adult ; Humans ; Podocytes/metabolism ; Tight Junctions/pathology ; Nephrosis, Lipoid/metabolism ; Nephrosis, Lipoid/pathology ; Intercellular Junctions/metabolism ; Recurrence
Language	English
Publishing date	2024-02-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	2943-9
ISSN	1525-2191 ; 0002-9440
ISSN (online)	1525-2191
ISSN	0002-9440
DOI	10.1016/j.ajpath.2024.01.008
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Article ; Online: ZnUMBA - a live imaging method to detect local barrier breaches.

Higashi, Tomohito / Stephenson, Rachel E / Schwayer, Cornelia / Huljev, Karla / Higashi, Atsuko Y / Heisenberg, Carl-Philipp / Chiba, Hideki / Miller, Ann L

Journal of cell science

2023 Volume 136, Issue 15

Abstract: Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage across the monolayer. Although these methods ... ...

Abstract	Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage across the monolayer. Although these methods measure changes in global barrier function, they lack the sensitivity needed to detect local or transient barrier breaches, and they do not reveal the location of barrier leaks. Therefore, we previously developed a method that we named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which overcomes these limitations, allowing for detection of local tight junction leaks with high spatiotemporal resolution. Here, we present expanded applications for ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier restoration and actin accumulation following laser injury. ZnUMBA can also be effectively utilized in developing zebrafish embryos as well as cultured monolayers of Madin-Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful and flexible method that, with minimal optimization, can be applied to multiple systems to measure dynamic changes in barrier function with spatiotemporal precision.
MeSH term(s)	Animals ; Dogs ; Epithelial Cells ; Zinc ; Zebrafish ; Madin Darby Canine Kidney Cells ; Tight Junctions ; Actins
Chemical Substances	Zinc (J41CSQ7QDS) ; Actins
Language	English
Publishing date	2023-08-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2993-2
ISSN	1477-9137 ; 0021-9533
ISSN (online)	1477-9137
ISSN	0021-9533
DOI	10.1242/jcs.260668
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Zs.A 1200: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland.

Higashi, Atsuko Y / Higashi, Tomohito / Furuse, Kyoko / Ozeki, Kana / Furuse, Mikio / Chiba, Hideki

Scientific reports

2021 Volume 11, Issue 1, Page(s) 21642

Abstract: The anterior pituitary gland regulates growth, metabolism, and reproduction by secreting hormones. Folliculo-stellate (FS) cells are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands. They form follicular lumens, ... ...

Abstract	The anterior pituitary gland regulates growth, metabolism, and reproduction by secreting hormones. Folliculo-stellate (FS) cells are non-endocrine cells located among hormone-producing cells in the anterior pituitary glands. They form follicular lumens, which are sealed by tight junctions (TJs). Although FS cells are hypothesized to contribute to fine-tuning of endocrine cells, little is known about the exact roles of FS cells. Here, we investigated the molecular composition of TJs in FS cells. We demonstrated that occludin is a good marker for TJs in the pituitary gland and examined the structure of the lumens surrounded by FS cells. We also found that claudin-9 is a major component of TJs in the FS cells. In immunoelectron microscopy, claudin-9 was specifically localized at TJs of the FS cells. The expression of claudin-9 was gradually increased in the pituitary gland after birth, suggesting that claudin-9 is developmentally regulated and performs some specific functions on the paracellular barrier of follicles in the pituitary gland. Furthermore, we found that angulin-1, angulin-2, and tricellulin are localized at the tricellular contacts of the FS cells. Our findings provide a first comprehensive molecular profile of TJs in the FS cells, and may lead us towards unveiling the FS cell functions.
MeSH term(s)	Animals ; Astrocytes/metabolism ; Cell Physiological Phenomena ; Claudins/metabolism ; Claudins/physiology ; Female ; Male ; Mice ; Mice, Inbred C57BL ; Occludin/metabolism ; Pituitary Gland/metabolism ; Pituitary Gland, Anterior/cytology ; Pituitary Gland, Anterior/metabolism ; Pituitary Gland, Anterior/physiology ; Tight Junctions/metabolism ; Tight Junctions/physiology
Chemical Substances	Claudins ; Cldn9 protein, mouse ; Occludin
Language	English
Publishing date	2021-11-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-01004-z
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Article ; Online: Expression Profiling of Fibroblasts in Chronic and Acute Disease Models Reveals Novel Pathways in Kidney Fibrosis.

Higashi, Atsuko Y / Aronow, Bruce J / Dressler, Gregory R

Journal of the American Society of Nephrology : JASN

2018 Volume 30, Issue 1, Page(s) 80–94

Abstract: Background: Renal interstitial fibrosis results from activation and proliferation of fibroblasts to myofibroblasts, secretion and accumulation of extracellular matrix, and displacement of normal renal tubules. In contrast to chronic renal disease, acute ...

Abstract	Background: Renal interstitial fibrosis results from activation and proliferation of fibroblasts to myofibroblasts, secretion and accumulation of extracellular matrix, and displacement of normal renal tubules. In contrast to chronic renal disease, acute injury may be repaired, a process that includes a decrease in the number of myofibroblasts in the interstitium and degradation of the accumulated extracellular matrix, leaving little evidence of prior injury. Methods: To investigate whether activated fibroblasts demonstrate changes in gene expression that correspond with regression after acute injury but are not observed in chronic models of fibrosis, we used microarrays to analyze gene expression patterns among fibroblast populations at different stages of injury or repair. We then mined the data for signaling pathways in fibroblasts corresponding to the acute proliferative, regression, and chronic phases of renal injury. Results: We identified multiple gene clusters with changes that correlate with the three phases of renal injury, including changes in levels of receptors for the antifibrotic factor PGE2. In adult renal fibroblast cultures, PGE2 was able to upregulate many genes that are suppressed by the profibrotic cytokine TGF- Conclusions: Inherent gene expression changes in activated fibroblasts accompany the transition from AKI to repair and regeneration. In chronic models, however, activated fibroblasts are resistant to the antifibrotic effects of PGE2 due to suppression of a subset of PGE receptors.
MeSH term(s)	Acute Kidney Injury/genetics ; Acute Kidney Injury/pathology ; Animals ; Cell Differentiation/genetics ; Cells, Cultured ; Dinoprostone/pharmacology ; Disease Models, Animal ; Extracellular Matrix/metabolism ; Fibroblasts/cytology ; Fibrosis/genetics ; Fibrosis/pathology ; Gene Expression Profiling ; Gene Expression Regulation ; Immunohistochemistry ; Mice ; Myofibroblasts/cytology ; Real-Time Polymerase Chain Reaction/methods ; Renal Insufficiency, Chronic/genetics ; Renal Insufficiency, Chronic/pathology ; Signal Transduction/genetics
Chemical Substances	Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	2018-12-13
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1085942-1
ISSN	1533-3450 ; 1046-6673
ISSN (online)	1533-3450
ISSN	1046-6673
DOI	10.1681/ASN.2018060644
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Article ; Online: EpCAM proteolysis and release of complexed claudin-7 repair and maintain the tight junction barrier.

Higashi, Tomohito / Saito, Akira C / Fukazawa, Yugo / Furuse, Mikio / Higashi, Atsuko Y / Ono, Masahiro / Chiba, Hideki

The Journal of cell biology

2022 Volume 222, Issue 1

Abstract: TJs maintain the epithelial barrier by regulating paracellular permeability. Since TJs are under dynamically fluctuating intercellular tension, cells must continuously survey and repair any damage. However, the underlying mechanisms allowing cells to ... ...

Abstract	TJs maintain the epithelial barrier by regulating paracellular permeability. Since TJs are under dynamically fluctuating intercellular tension, cells must continuously survey and repair any damage. However, the underlying mechanisms allowing cells to sense TJ damage and repair the barrier are not yet fully understood. Here, we showed that proteinases play an important role in the maintenance of the epithelial barrier. At TJ break sites, EpCAM-claudin-7 complexes on the basolateral membrane become accessible to apical membrane-anchored serine proteinases (MASPs) and the MASPs cleave EpCAM. Biochemical data and imaging analysis suggest that claudin-7 released from EpCAM contributes to the rapid repair of damaged TJs. Knockout (KO) of MASPs drastically reduced barrier function and live-imaging of TJ permeability showed that MASPs-KO cells exhibited increased size, duration, and frequency of leaks. Together, our results reveal a novel mechanism of TJ maintenance through the localized proteolysis of EpCAM at TJ leaks, and provide a better understanding of the dynamic regulation of epithelial permeability.
MeSH term(s)	Claudins/genetics ; Claudins/metabolism ; Epithelial Cell Adhesion Molecule/genetics ; Epithelial Cell Adhesion Molecule/metabolism ; Mannose-Binding Protein-Associated Serine Proteases/metabolism ; Proteolysis ; Tight Junctions/metabolism ; Gene Knockout Techniques
Chemical Substances	Claudins ; Epithelial Cell Adhesion Molecule ; Mannose-Binding Protein-Associated Serine Proteases (EC 3.4.21.-)
Language	English
Publishing date	2022-11-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218154-x
ISSN	1540-8140 ; 0021-9525
ISSN (online)	1540-8140
ISSN	0021-9525
DOI	10.1083/jcb.202204079
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Article ; Online: Occludin and tricellulin facilitate formation of anastomosing tight-junction strand network to improve barrier function.

Saito, Akira C / Higashi, Tomohito / Fukazawa, Yugo / Otani, Tetsuhisa / Tauchi, Masashi / Higashi, Atsuko Y / Furuse, Mikio / Chiba, Hideki

Molecular biology of the cell

2021 Volume 32, Issue 8, Page(s) 722–738

Abstract: Tight junctions (TJs) are composed of a claudin-based anastomosing network of TJ strands at which plasma membranes of adjacent epithelial cells are closely attached to regulate the paracellular permeability. Although the TJ proteins occludin and ... ...

Abstract	Tight junctions (TJs) are composed of a claudin-based anastomosing network of TJ strands at which plasma membranes of adjacent epithelial cells are closely attached to regulate the paracellular permeability. Although the TJ proteins occludin and tricellulin have been known to be incorporated in the TJ strand network, their molecular functions remain unknown. Here, we established tricellulin/occludin-double knockout (dKO) MDCK II cells using a genome editing technique and evaluated the structure and barrier function of these cells. In freeze-fracture replica electron microscopy, the TJ strands of tricellulin/occludin-dKO cells had fewer branches and were less anastomosed compared with the controls. The paracellular permeability of ions and small tracers was increased in the dKO cells. A single KO of tricellulin or occludin had limited effects on the morphology and permeability of TJs. Mathematical simulation using a simplified TJ strand network model predicted that reduced cross-links in TJ strands lead to increased permeability of ions and small macromolecules. Furthermore, overexpression of occludin increased the complexity of TJ strand network and strengthened barrier function. Taken together, our data suggest that tricellulin and occludin mediate the formation and/or stabilization of TJ-strand branching points and contribute to the maintenance of epithelial barrier integrity.
MeSH term(s)	Animals ; Cell Line ; Claudins/metabolism ; Dogs ; Epithelial Cells/metabolism ; HEK293 Cells ; Humans ; MARVEL Domain Containing 2 Protein/metabolism ; MARVEL Domain Containing 2 Protein/physiology ; Madin Darby Canine Kidney Cells ; Occludin/metabolism ; Occludin/physiology ; Tight Junctions/metabolism ; Tight Junctions/physiology
Chemical Substances	Claudins ; MARVEL Domain Containing 2 Protein ; MARVELD2 protein, human ; OCLN protein, human ; Occludin
Language	English
Publishing date	2021-02-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1098979-1
ISSN	1939-4586 ; 1059-1524
ISSN (online)	1939-4586
ISSN	1059-1524
DOI	10.1091/mbc.E20-07-0464
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Zs.A 2853: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Claudin‑9 is a novel prognostic biomarker for endometrial cancer.

Endo, Yuta / Sugimoto, Kotaro / Kobayashi, Makoto / Kobayashi, Yasuyuki / Kojima, Manabu / Furukawa, Shigenori / Soeda, Shu / Watanabe, Takafumi / Higashi, Atsuko Y / Higashi, Tomohito / Hashimoto, Yuko / Fujimori, Keiya / Chiba, Hideki

International journal of oncology

2022 Volume 61, Issue 5

Abstract: The tight‑junction protein claudin‑9 (CLDN9) is barely distributed in normal adult tissues but is ectopically expressed in various cancer types. Although multiple databases indicated upregulation of CLDN9 in endometrial cancers at the mRNA level, its ... ...

Abstract	The tight‑junction protein claudin‑9 (CLDN9) is barely distributed in normal adult tissues but is ectopically expressed in various cancer types. Although multiple databases indicated upregulation of CLDN9 in endometrial cancers at the mRNA level, its protein expression and biological roles remain obscure. In the present study, the prognostic significance of CLDN9 expression in endometrial cancer was evaluated by immunohistochemical staining and semi‑quantification using formalin‑fixed paraffin‑embedded specimens obtained from 248 endometrial carcinoma cases. A total of 43 cases (17.3%) had high CLDN9 expression, whereas 205 cases (82.7%) exhibited low CLDN9 expression. The 5‑year disease‑specific survival rates in the high and low CLDN9 expression groups were 62.8 and 87.8% (P<0.001), respectively. In addition, multivariate analysis revealed that high CLDN9 expression was an independent prognostic factor (hazard ratio, 4.99; 95% CI, 1.96‑12.70; P<0.001). Furthermore, CLDN9 expression was significantly correlated with the expression of CLDN6 (P<0.001), which is the closest CLDN member to CLDN9 and a poor prognostic factor for endometrial carcinoma. The 5‑year disease‑specific survival rate of cases with CLDN6‑high/CLDN9‑high, CLDN6‑high/CLDN9‑low and CLDN6‑low/CLDN9‑high status was 30.0, 37.5 and 72.7%, respectively, whereas that of CLDN6‑low/CLDN9‑low was 89.8% (P=0.004). In conclusion, aberrant CLDN9 expression is a predictor of poor prognosis for endometrial cancer and may be utilized in combination with CLDN6 to achieve higher sensitivity.
MeSH term(s)	Biomarkers ; Claudins/genetics ; Claudins/metabolism ; Endometrial Neoplasms/genetics ; Endometrial Neoplasms/pathology ; Female ; Formaldehyde ; Humans ; Prognosis ; RNA, Messenger/metabolism
Chemical Substances	Biomarkers ; CLDN9 protein, human ; Claudins ; RNA, Messenger ; Formaldehyde (1HG84L3525)
Language	English
Publishing date	2022-09-21
Publishing country	Greece
Document type	Journal Article
ZDB-ID	1154403-x
ISSN	1791-2423 ; 1019-6439
ISSN (online)	1791-2423
ISSN	1019-6439
DOI	10.3892/ijo.2022.5425
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Article ; Online: Pax2 and Pax8 Proteins Regulate Urea Transporters and Aquaporins to Control Urine Concentration in the Adult Kidney.

Laszczyk, Ann M / Higashi, Atsuko Y / Patel, Sanjeevkumar R / Johnson, Craig N / Soofi, Abdul / Abraham, Saji / Dressler, Gregory R

Journal of the American Society of Nephrology : JASN

2020 Volume 31, Issue 6, Page(s) 1212–1225

Abstract: Background: As the glomerular filtrate passes through the nephron and into the renal medulla, electrolytes, water, and urea are reabsorbed through the concerted actions of solute carrier channels and aquaporins at various positions along the nephron and ...

Abstract	Background: As the glomerular filtrate passes through the nephron and into the renal medulla, electrolytes, water, and urea are reabsorbed through the concerted actions of solute carrier channels and aquaporins at various positions along the nephron and in the outer and inner medulla. Proliferating stem cells expressing the nuclear transcription factor Pax2 give rise to renal epithelial cells. Pax2 expression ends once the epithelial cells differentiate into mature proximal and distal tubules, whereas expression of the related Pax8 protein continues. The collecting tubules and renal medulla are derived from Pax2-positive ureteric bud epithelia that continue to express Pax2 and Pax8 in adult kidneys. Despite the crucial role of Pax2 in renal development, functions for Pax2 or Pax8 in adult renal epithelia have not been established. Methods: To examine the roles of Pax2 and Pax8 in the adult mouse kidney, we deleted either Results: Mice with induced deletions of both Conclusions: These data reveal novel functions for Pax proteins in adult renal epithelia that are essential for retaining water and concentrating urine.
MeSH term(s)	Animals ; Aquaporins/physiology ; Female ; HEK293 Cells ; Humans ; Kidney/physiology ; Kidney Concentrating Ability/physiology ; Male ; Membrane Transport Proteins/physiology ; Mice ; Osmoregulation ; PAX2 Transcription Factor/genetics ; PAX2 Transcription Factor/physiology ; PAX8 Transcription Factor/genetics ; PAX8 Transcription Factor/physiology ; Urea Transporters
Chemical Substances	Aquaporins ; Membrane Transport Proteins ; PAX2 Transcription Factor ; PAX8 Transcription Factor ; Pax2 protein, mouse ; Pax8 protein, mouse
Language	English
Publishing date	2020-05-07
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1085942-1
ISSN	1533-3450 ; 1046-6673
ISSN (online)	1533-3450
ISSN	1046-6673
DOI	10.1681/ASN.2019090962
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Article ; Online: CLDN15 is a novel diagnostic marker for malignant pleural mesothelioma.

Watanabe, Masayuki / Higashi, Tomohito / Ozeki, Kana / Higashi, Atsuko Y / Sugimoto, Kotaro / Mine, Hayato / Takagi, Hironori / Ozaki, Yuki / Muto, Satoshi / Okabe, Naoyuki / Matsumura, Yuki / Hasegawa, Takeo / Shio, Yutaka / Suzuki, Hiroyuki / Chiba, Hideki

Scientific reports

2021 Volume 11, Issue 1, Page(s) 12554

Abstract: Malignant mesothelioma is a cancer with a poor survival rate. It is difficult to diagnose mesotheliomas because they show a variety of histological patterns similar to those of various other cancers. However, since currently used positive markers for ... ...

Abstract	Malignant mesothelioma is a cancer with a poor survival rate. It is difficult to diagnose mesotheliomas because they show a variety of histological patterns similar to those of various other cancers. However, since currently used positive markers for mesotheliomas may show false positives or false negatives, a novel mesothelial positive marker is required. In the present study, we screened 25 claudins and found that claudin-15 is expressed in the mesothelial cells. We made new rat anti-human claudin-15 (CLDN15) monoclonal antibodies that selectively recognize CLDN15, and investigated whether CLDN15 is a good positive marker for malignant pleural mesotheliomas (MPMs) using MPM tissue samples by immunohistochemistry and semi-quantification of the expression level using an immunoreactive score (IRS) method. Of 42 MPM samples, 83% were positive for CLDN15. The positive ratio was equal to or greater than other positive markers for MPMs including calretinin (81%), WT-1 (50%), and D2-40 (81%). In 50 lung adenocarcinoma sections, four cases were positive for CLDN15 and the specificity (92%) was comparable with other markers (90-100%). Notably, CLDN15 was rarely detected in 24 non-mesothelial tumors in the tissue microarray (12/327 cases). In conclusion, CLDN15 can be used in the clinical setting as a positive marker for MPM diagnosis.
MeSH term(s)	Adenocarcinoma of Lung/diagnosis ; Adenocarcinoma of Lung/genetics ; Adenocarcinoma of Lung/pathology ; Adult ; Aged ; Aged, 80 and over ; Animals ; Biomarkers, Tumor/genetics ; Calbindin 2/genetics ; Claudins/genetics ; Diagnosis, Differential ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; Mesothelioma, Malignant/diagnosis ; Mesothelioma, Malignant/genetics ; Mesothelioma, Malignant/pathology ; Middle Aged ; Rats ; WT1 Proteins/genetics
Chemical Substances	Biomarkers, Tumor ; CALB2 protein, human ; Calbindin 2 ; Claudins ; WT1 Proteins ; WT1 protein, human ; claudin 15
Language	English
Publishing date	2021-06-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-91464-0
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Article ; Online: From Otic Induction to Hair Cell Production: Pax2

Schaefer, Stacy A / Higashi, Atsuko Y / Loomis, Benjamin / Schrepfer, Thomas / Wan, Guoqiang / Corfas, Gabriel / Dressler, Gregory R / Duncan, Robert Keith

Stem cells and development

2018 Volume 27, Issue 4, Page(s) 237–251

Abstract: Producing hair cells of the inner ear is the major goal of ongoing research that combines advances in developmental and stem cell biology. The recent advent of an inner ear organoid protocol-resulting in three-dimensional stem cell-derived tissues ... ...

Abstract	Producing hair cells of the inner ear is the major goal of ongoing research that combines advances in developmental and stem cell biology. The recent advent of an inner ear organoid protocol-resulting in three-dimensional stem cell-derived tissues resembling vestibular sensory epithelia-has sparked interest in applications such as regeneration, drug discovery, and disease modeling. In this study, we adapted this protocol for a novel mouse embryonic stem cell line with a fluorescent reporter for Pax2 expression. We used Pax2
MeSH term(s)	Animals ; Cell Differentiation/genetics ; Cell Line ; Ear, Inner/cytology ; Ear, Inner/growth & development ; Ear, Inner/metabolism ; Gene Expression Regulation, Developmental ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Hair Cells, Auditory/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Microscopy, Fluorescence ; Mouse Embryonic Stem Cells/cytology ; Mouse Embryonic Stem Cells/metabolism ; Organogenesis/genetics ; Organoids/cytology ; Organoids/metabolism ; PAX2 Transcription Factor/genetics ; PAX2 Transcription Factor/metabolism
Chemical Substances	PAX2 Transcription Factor ; Green Fluorescent Proteins (147336-22-9)
Language	English
Publishing date	2018-01-29
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2142214-X
ISSN	1557-8534 ; 1547-3287
ISSN (online)	1557-8534
ISSN	1547-3287
DOI	10.1089/scd.2017.0142
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Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito