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  1. Article ; Online: Purification and concentration of non-infectious West Nile virus-like particles and infectious virions using a pseudo-affinity Cellufine Sulfate column.

    Ohtaki, Naohiro / Takahashi, Hidehiro / Kaneko, Keiko / Gomi, Yasuyuki / Ishikawa, Toyokazu / Higashi, Yasushi / Todokoro, Masami / Kurata, Takeshi / Sata, Tetsutaro / Kojima, Asato

    Journal of virological methods

    2011  Volume 174, Issue 1-2, Page(s) 131–135

    Abstract: Affinity column chromatography is a promising method for the purification of flavivirus particles that can supplement or potentially replace diafiltration and sucrose density centrifugation. In this study, the purification of West Nile Virus (WNV) ... ...

    Abstract Affinity column chromatography is a promising method for the purification of flavivirus particles that can supplement or potentially replace diafiltration and sucrose density centrifugation. In this study, the purification of West Nile Virus (WNV) antigens via Cellufine Sulfate column chromatography was examined. Virus-like particles (VLPs) produced by the expression of the prM and E genes were separated from most of the contaminant proteins with 0.2-0.4M NaCl, but still retained their spherical forms and immunogenicity in mice. The column, with a 1 mL bed-volume, concentrated WN-VLPs a minimum of 15 fold from culture supernatants. A heparin analogue, suramin, competitively eluted WN-VLPs, but sulphated polysaccharides, such as heparin, heparin sulfate and dextran sulfate, did not. Furthermore, 2.4 × 10⁹ plaque forming units of WNV and 196 μg of the viral antigens were recovered from 60 mL of infected culture medium at high yields (93% and 96%, respectively). These results indicate that, in addition to conventional methods, Cellufine Sulfate column chromatography is an effective preparation technique for WNV particulate antigens that does not impair the antigen virological characteristics.
    MeSH term(s) Animals ; Chromatography, Affinity/methods ; Chromatography, Liquid/methods ; Mice ; Virion/isolation & purification ; Virology/methods ; Virosomes/isolation & purification ; West Nile virus/isolation & purification
    Chemical Substances Virosomes
    Language English
    Publishing date 2011-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2011.03.021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1565: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Immunogenicity and efficacy of two types of West Nile virus-like particles different in size and maturation as a second-generation vaccine candidate.

    Ohtaki, Naohiro / Takahashi, Hidehiro / Kaneko, Keiko / Gomi, Yasuyuki / Ishikawa, Toyokazu / Higashi, Yasushi / Kurata, Takeshi / Sata, Tetsutaro / Kojima, Asato

    Vaccine

    2010  Volume 28, Issue 40, Page(s) 6588–6596

    Abstract: Virus-like particles (VLPs) of flaviviruses generated from the prM and E genes are a promising vaccine candidate. We have established cell clones continuously releasing VLPs of West Nile virus (WNV) in serum-free conditions. Two types of VLPs were ... ...

    Abstract Virus-like particles (VLPs) of flaviviruses generated from the prM and E genes are a promising vaccine candidate. We have established cell clones continuously releasing VLPs of West Nile virus (WNV) in serum-free conditions. Two types of VLPs were distinguished by sedimenting analyses in sucrose density gradients. Fast sedimenting VLPs (F-VLPs) were large (40-50 nm) and composed of the E and processed mature M proteins, whereas slowly sedimenting VLPs (S-VLPs) were small (20-30 nm) particles consisting of the E and immature prM proteins. F-VLPs induced higher neutralizing antibody and anti-WNV IgG titers than S-VLPs. Furthermore, IgG2a was dominant over IgG1 by immunization with F-VLPs as with whole virion-derived antigens. Mice vaccinated with a low dose (3 ng) of F-VLPs showed higher protective efficacy (83% survivals) against WNV infection than S-VLP-immune mice (17% survivals). These results indicate that F-VLPs more closely resemble the virions and take a better immunogenic form than S-VLPs as WNV vaccine candidates.
    MeSH term(s) Animals ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; Antibody Formation ; CHO Cells ; Centrifugation, Density Gradient ; Cricetinae ; Cricetulus ; Immunoglobulin G/blood ; Mice ; Mice, Inbred C3H ; Particle Size ; Vaccines, Virus-Like Particle/immunology ; Viral Envelope Proteins/immunology ; Viral Matrix Proteins/immunology ; West Nile Fever/immunology ; West Nile Fever/prevention & control ; West Nile Virus Vaccines/immunology ; West Nile virus/immunology
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Immunoglobulin G ; Vaccines, Virus-Like Particle ; Viral Envelope Proteins ; Viral Matrix Proteins ; West Nile Virus Vaccines
    Language English
    Publishing date 2010-09-14
    Publishing country Netherlands
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2010.07.055
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1930: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 458: Show issues

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  3. Article: [Lymph nodes mapping for detection of sentinel nodes in patients with papillary thyroid cancer].

    Nakano, Shizuo / Uenosono, Yoshikazu / Ehi, Katsuhiko / Arigami, Takaaki / Higashi, Yasushi / Maeda, Tetsu / Kijima, Yuuko / Natsugoe, Shouji / Aikou, Takashi

    Gan to kagaku ryoho. Cancer & chemotherapy

    2004  Volume 31, Issue 5, Page(s) 801–804

    Abstract: We examined the feasibility of sentinel lymph node biopsy for papillary thyroid cancer. In the dye injection method, 1% of isosulfan blue dye was injected around the tumor of 32 patients intra-operatively, and in the radioisotope (RI) colloid injection ... ...

    Abstract We examined the feasibility of sentinel lymph node biopsy for papillary thyroid cancer. In the dye injection method, 1% of isosulfan blue dye was injected around the tumor of 32 patients intra-operatively, and in the radioisotope (RI) colloid injection method, 99mTc-tin colloid was injected in 23 patients 1 day preoperatively. Lymph node mapping for detection of sentinel nodes was performed after thyroidectomy and central and modified lateral neck lymph node dissections. All dissected nodes were examined postoperatively by hematoxylineosin staining to determine whether or not metastasis was present. In the dye injection method, sentinel lymph nodes were identified in 30 (94%) of the 32 patients. Lymph node metastases were found in 14 patients, and some sentinel lymph nodes had papillary cancer metastasis in 13 patients. There was only 1 false-negative case. Sensitivity and accuracy of sentinel lymph node biopsy was 93% (13/14) and 97% (29/30). With the RI method, detection rate, sensitivity and accuracy of sentinel lymph node biopsy was 96% (22/23), 90% (9/10) and 95% (21/22), respectively. Our preliminary study indicated that sentinel lymph node biopsy was feasible in patients with thyroid cancer. It may be helpful in avoiding unnecessary lymph node dissection and improving quality of life in patients with thyroid cancer.
    MeSH term(s) Carcinoma, Papillary/pathology ; Carcinoma, Papillary/surgery ; Humans ; Lymph Node Excision ; Lymph Nodes/pathology ; Lymphatic Metastasis ; Radiopharmaceuticals ; Risk Assessment ; Rosaniline Dyes ; Sensitivity and Specificity ; Sentinel Lymph Node Biopsy ; Technetium Tc 99m Sulfur Colloid ; Thyroid Neoplasms/pathology ; Thyroid Neoplasms/surgery ; Thyroidectomy ; Treatment Outcome
    Chemical Substances Radiopharmaceuticals ; Rosaniline Dyes ; iso-sulfan blue (39N9K8S2A4) ; Technetium Tc 99m Sulfur Colloid (556Q0P6PB1)
    Language Japanese
    Publishing date 2004-05
    Publishing country Japan
    Document type English Abstract ; Journal Article
    ZDB-ID 604842-0
    ISSN 0385-0684
    ISSN 0385-0684
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2573: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Purification and concentration of non-infectious West Nile virus-like particles and infectious virions using a pseudo-affinity Cellufine Sulfate column

    Ohtaki, Naohiro / Takahashi, Hidehiro / Kaneko, Keiko / Gomi, Yasuyuki / Ishikawa, Toyokazu / Higashi, Yasushi / Todokoro, Masami / Kurata, Takeshi / Sata, Tetsutaro / Kojima, Asato

    Journal of virological methods

    Volume v. 174,, Issue no. 1

    Abstract: Affinity column chromatography is a promising method for the purification of flavivirus particles that can supplement or potentially replace diafiltration and sucrose density centrifugation. In this study, the purification of West Nile Virus (WNV) ... ...

    Abstract Affinity column chromatography is a promising method for the purification of flavivirus particles that can supplement or potentially replace diafiltration and sucrose density centrifugation. In this study, the purification of West Nile Virus (WNV) antigens via Cellufine Sulfate column chromatography was examined. Virus-like particles (VLPs) produced by the expression of the prM and E genes were separated from most of the contaminant proteins with 0.2–0.4M NaCl, but still retained their spherical forms and immunogenicity in mice. The column, with a 1mL bed-volume, concentrated WN-VLPs a minimum of 15 fold from culture supernatants. A heparin analogue, suramin, competitively eluted WN-VLPs, but sulphated polysaccharides, such as heparin, heparin sulfate and dextran sulfate, did not. Furthermore, 2.4×10⁹ plaque forming units of WNV and 196μg of the viral antigens were recovered from 60mL of infected culture medium at high yields (93% and 96%, respectively). These results indicate that, in addition to conventional methods, Cellufine Sulfate column chromatography is an effective preparation technique for WNV particulate antigens that does not impair the antigen virological characteristics.
    Keywords virion ; chromatography ; sodium chloride ; viral antigens ; sulfates ; centrifugation ; dextran ; genes ; sucrose ; mice ; virus-like particles ; suramin ; purification methods ; culture media ; heparin ; immune response ; proteins ; West Nile virus
    Language English
    Document type Article
    ISSN 0166-0934
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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