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  1. Article ; Online: Membrane Traffic in

    Higuchi, Yujiro

    Journal of fungi (Basel, Switzerland)

    2021  Volume 7, Issue 7

    Abstract: The industrially important filamentous ... ...

    Abstract The industrially important filamentous fungus
    Language English
    Publishing date 2021-07-01
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof7070534
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  2. Article ; Online: Membrane traffic related to endosome dynamics and protein secretion in filamentous fungi.

    Higuchi, Yujiro

    Bioscience, biotechnology, and biochemistry

    2021  Volume 85, Issue 5, Page(s) 1038–1045

    Abstract: In eukaryotic cells, membrane-surrounded organelles are orchestrally organized spatiotemporally under environmental situations. Among such organelles, vesicular transports and membrane contacts occur to communicate each other, so-called membrane traffic. ...

    Abstract In eukaryotic cells, membrane-surrounded organelles are orchestrally organized spatiotemporally under environmental situations. Among such organelles, vesicular transports and membrane contacts occur to communicate each other, so-called membrane traffic. Filamentous fungal cells are highly polarized and thus membrane traffic is developed to have versatile functions. Early endosome (EE) is an endocytic organelle that dynamically exhibits constant long-range motility through the hyphal cell, which is proven to have physiological roles, such as other organelle distribution and signal transduction. Since filamentous fungal cells are also considered as cell factories, to produce valuable proteins extracellularly, molecular mechanisms of secretory pathway including protein glycosylation have been well investigated. In this review, molecular and physiological aspects of membrane traffic especially related to EE dynamics and protein secretion in filamentous fungi are summarized, and perspectives for application are also described.
    MeSH term(s) Cell Compartmentation ; Cell Membrane/metabolism ; Cell Membrane/ultrastructure ; Cell Polarity ; Endocytosis ; Endosomes/metabolism ; Endosomes/ultrastructure ; Fungal Proteins/biosynthesis ; Fungal Proteins/metabolism ; Fungi/metabolism ; Fungi/ultrastructure ; Glycosylation ; Golgi Apparatus/metabolism ; Golgi Apparatus/ultrastructure ; Hyphae/metabolism ; Hyphae/ultrastructure ; Microtubules/metabolism ; Microtubules/ultrastructure ; Protein Biosynthesis ; Protein Processing, Post-Translational ; Protein Transport ; Secretory Vesicles/metabolism ; Secretory Vesicles/ultrastructure ; Signal Transduction
    Chemical Substances Fungal Proteins
    Language English
    Publishing date 2021-02-26
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1106450-x
    ISSN 1347-6947 ; 0916-8451
    ISSN (online) 1347-6947
    ISSN 0916-8451
    DOI 10.1093/bbb/zbab004
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4647: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  3. Article ; Online: Clinical genetics of Charcot-Marie-Tooth disease.

    Higuchi, Yujiro / Takashima, Hiroshi

    Journal of human genetics

    2022  Volume 68, Issue 3, Page(s) 199–214

    Abstract: Recent research in the field of inherited peripheral neuropathies (IPNs) such as Charcot-Marie-Tooth (CMT) disease has helped identify the causative genes provided better understanding of the pathogenesis, and unraveled potential novel therapeutic ... ...

    Abstract Recent research in the field of inherited peripheral neuropathies (IPNs) such as Charcot-Marie-Tooth (CMT) disease has helped identify the causative genes provided better understanding of the pathogenesis, and unraveled potential novel therapeutic targets. Several reports have described the epidemiology, clinical characteristics, molecular pathogenesis, and novel causative genes for CMT/IPNs in Japan. Based on the functions of the causative genes identified so far, the following molecular and cellular mechanisms are believed to be involved in the causation of CMTs/IPNs: myelin assembly, cytoskeletal structure, myelin-specific transcription factor, nuclear related, endosomal sorting and cell signaling, proteasome and protein aggregation, mitochondria-related, motor proteins and axonal transport, tRNA synthetases and RNA metabolism, and ion channel-related mechanisms. In this article, we review the epidemiology, genetic diagnosis, and clinicogenetic characteristics of CMT in Japan. In addition, we discuss the newly identified novel causative genes for CMT/IPNs in Japan, namely MME and COA7. Identification of the new causes of CMT will facilitate in-depth characterization of the underlying molecular mechanisms of CMT, leading to the establishment of therapeutic approaches such as drug development and gene therapy.
    MeSH term(s) Humans ; Charcot-Marie-Tooth Disease/genetics ; Myelin Sheath ; Transcription Factors/genetics ; Mitochondrial Proteins/genetics ; Mutation
    Chemical Substances Transcription Factors ; Mitochondrial Proteins
    Language English
    Publishing date 2022-03-18
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1425192-9
    ISSN 1435-232X ; 1434-5161
    ISSN (online) 1435-232X
    ISSN 1434-5161
    DOI 10.1038/s10038-022-01031-2
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  4. Article ; Online: Polarity-dependent expression and localization of secretory glucoamylase mRNA in filamentous fungal cells.

    Morita, Yuki / Takegawa, Kaoru / Collins, Brett M / Higuchi, Yujiro

    Microbiological research

    2024  Volume 282, Page(s) 127653

    Abstract: In multinuclear and multicellular filamentous fungi little is known about how mRNAs encoding secreted enzymes are transcribed and localized spatiotemporally. To better understand this process we analyzed mRNA encoding GlaA, a glucoamylase secreted in ... ...

    Abstract In multinuclear and multicellular filamentous fungi little is known about how mRNAs encoding secreted enzymes are transcribed and localized spatiotemporally. To better understand this process we analyzed mRNA encoding GlaA, a glucoamylase secreted in large amounts by the industrial filamentous fungus Aspergillus oryzae, by the MS2 system, in which mRNA can be visualized in living cells. We found that glaA mRNA was significantly transcribed and localized near the hyphal tip and septum, which are the sites of protein secretion, in polarity-dependent expression and localization manners. We also revealed that glaA mRNA exhibits long-range dynamics in the vicinity of the endoplasmic reticulum (ER) in a manner that is dependent on the microtubule motor proteins kinesin-1 and kinesin-3, but independent of early endosomes. Moreover, we elucidated that although glaA mRNA localized to stress granules (SGs) and processing bodies (PBs) under high temperature, glaA mRNA was not seen under ER stress, suggesting that there are different regulatory mechanisms of glaA mRNA by SG and PB under high temperature and ER stress. Collectively, this study uncovers a dynamic regulatory mechanism of mRNA encoding a secretory enzyme in filamentous fungi.
    MeSH term(s) Glucan 1,4-alpha-Glucosidase/genetics ; Glucan 1,4-alpha-Glucosidase/metabolism ; Kinesins/metabolism ; Endoplasmic Reticulum/metabolism ; Protein Transport ; Fungi/metabolism ; Fungal Proteins/genetics ; Fungal Proteins/metabolism
    Chemical Substances Glucan 1,4-alpha-Glucosidase (EC 3.2.1.3) ; Kinesins (EC 3.6.4.4) ; Fungal Proteins
    Language English
    Publishing date 2024-02-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1189614-0
    ISSN 1618-0623 ; 0944-5013
    ISSN (online) 1618-0623
    ISSN 0944-5013
    DOI 10.1016/j.micres.2024.127653
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  5. Article ; Online: Traditional and Latest Researches on

    Higuchi, Yujiro / Kitamoto, Katsuhiko

    Journal of fungi (Basel, Switzerland)

    2021  Volume 7, Issue 12

    Abstract: We would like to thank all the contributors to this Special Issue ... ...

    Abstract We would like to thank all the contributors to this Special Issue on
    Language English
    Publishing date 2021-12-14
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof7121075
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  6. Article: Initial fungal effector production is mediated by early endosome motility.

    Higuchi, Yujiro

    Communicative & integrative biology

    2015  Volume 8, Issue 2, Page(s) e1025187

    Abstract: Fungal plant pathogenicity is facilitated by effector proteins that are specifically expressed during infection and are responsible for suppressing plant defense mechanisms. Recent studies have elucidated the detailed molecular mechanisms of effector ... ...

    Abstract Fungal plant pathogenicity is facilitated by effector proteins that are specifically expressed during infection and are responsible for suppressing plant defense mechanisms. Recent studies have elucidated the detailed molecular mechanisms of effector action throughout fungal infection. However, little is known about the trafficking and secretion of effectors in fungal hyphae during the initial stage of infection. Using state-of-the-art microscopy we have demonstrated that early endosome (EE) motility is required for effector production during fungal infection. Moreover, the MAPK Crk1 has been shown to travel on EEs and to function as a negative regulator of effector expression, suggesting that motile EEs are involved in signal transduction. Here I further discuss possible mechanisms whereby EE motility regulates effector expression in the initial stages of infection.
    Language English
    Publishing date 2015-04-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2451097-X
    ISSN 1942-0889
    ISSN 1942-0889
    DOI 10.1080/19420889.2015.1025187
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  7. Article: Single-Molecule FISH Reveals Subcellular Localization of α-Amylase and Actin mRNAs in the Filamentous Fungus

    Higuchi, Yujiro / Takegawa, Kaoru

    Frontiers in microbiology

    2020  Volume 11, Page(s) 578862

    Abstract: The machinery for mRNA localization is one of crucial molecular structures allowing cellular spatiotemporal organization of protein synthesis. Although the molecular mechanisms underlying mRNA localization have been thoroughly investigated in unicellular ...

    Abstract The machinery for mRNA localization is one of crucial molecular structures allowing cellular spatiotemporal organization of protein synthesis. Although the molecular mechanisms underlying mRNA localization have been thoroughly investigated in unicellular organisms, little is known about multicellular and multinuclear filamentous fungi. Here, we conducted single-molecule fluorescence
    Language English
    Publishing date 2020-09-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.578862
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  8. Article ; Online: Mechanistic insights into Schizosaccharomyces pombe GT-A family protein Pvg3 in the biosynthesis of pyruvylated β1,3-galactose of N-linked oligosaccharides

    Fukunaga, Takamasa / Watanabe, Masahiro / Nakamichi, Yusuke / Morita, Tomotake / Higuchi, Yujiro / Maekawa, Hiromi / Takegawa, Kaoru

    The Society for Biotechnology, Japan Journal of Bioscience and Bioengineering. 2023 June, v. 135, no. 6 p.423-432

    2023  

    Abstract: N-linked oligosaccharides in the fission yeast Schizosaccharomyces pombe contain large amounts of d-galactose (Gal), which mainly comprises α1,2- and α1,3-linked Gal except for pyruvylated β1,3-linked Gal (PvGalβ) at the non-reducing end. The PvGalβ unit ...

    Abstract N-linked oligosaccharides in the fission yeast Schizosaccharomyces pombe contain large amounts of d-galactose (Gal), which mainly comprises α1,2- and α1,3-linked Gal except for pyruvylated β1,3-linked Gal (PvGalβ) at the non-reducing end. The PvGalβ unit of N-glycans is important for regulating nonsexual flocculation and invasive growth, but the mechanistic basis for β-galactosylation in fission yeast is poorly understood. To gain insight into this mechanism, we have characterized three genes previously identified to be involved in PvGalβ biosynthesis (pvg2, pvg3, and pvg5), with a focus on pvg3, which is predicted to contain a domain conserved in galactosyltransferase family 31 (GT31) proteins. Fluorescent microscopy revealed that Pvg3 is stably localized at the Golgi membrane, regardless of the presence of pvg2⁺ or pvg5⁺, suggesting that Pvg2 and Pvg5 are essential for the function of Pvg3 as a β1,3-galactosyltransferase, and not for its localization to the Golgi. Mutation of the GT31 family DXD motif and GT-A fold in Pvg3 resulted in loss of catalytic activity in vivo, supporting the idea that Pvg3 is a GT-A type β1,3-galactosyltransferase. Docking simulations further indicated that Pvg3 can recognize donor and acceptor substrates suitable for β-(1→3) bond formation. Yeast two-hybrid assay showed that Pvg5 physically interacts with Pvg3 and the pyruvyltransferase Pvg1. Collectively, these results provide insight into β-galactosylation catalyzed by Pvg3 and the supporting role of Pvg5 in PvGalβ biosynthesis.
    Keywords Schizosaccharomyces pombe ; biosynthesis ; catalytic activity ; flocculation ; fluorescence microscopy ; galactose ; mutation ; oligosaccharides ; two hybrid system techniques ; GT-A family ; N-glycan ; Pyruvylated galactose ; Galactosyltransferase ; Fission yeast
    Language English
    Dates of publication 2023-06
    Size p. 423-432.
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 1465387-4
    ISSN 1347-4421 ; 1389-1723
    ISSN (online) 1347-4421
    ISSN 1389-1723
    DOI 10.1016/j.jbiosc.2023.03.002
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4728: Show issues

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  9. Article ; Online: Correlative Localization Analysis Between mRNA and Enhanced Green Fluorescence Protein-Fused Protein by a Single-Molecule Fluorescence

    Morita, Yuki / Katakura, Yoshinori / Takegawa, Kaoru / Higuchi, Yujiro

    Frontiers in fungal biology

    2021  Volume 2, Page(s) 721398

    Abstract: Although subcellular localization analysis of proteins fused with enhanced green fluorescence protein (EGFP) has been widely conducted in filamentous fungi, little is known about the localization of messenger RNAs (mRNAs) encoding the EGFP-fused proteins. ...

    Abstract Although subcellular localization analysis of proteins fused with enhanced green fluorescence protein (EGFP) has been widely conducted in filamentous fungi, little is known about the localization of messenger RNAs (mRNAs) encoding the EGFP-fused proteins. In this study, we performed single-molecule fluorescence
    Language English
    Publishing date 2021-10-13
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-6128
    ISSN (online) 2673-6128
    DOI 10.3389/ffunb.2021.721398
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  10. Article ; Online: A novel homozygous HPDL variant in Japanese siblings with autosomal recessive hereditary spastic paraplegia: case report and literature review.

    Kojima, Fumikazu / Okamoto, Yuji / Ando, Masahiro / Higuchi, Yujiro / Hobara, Takahiro / Yuan, Junhui / Yoshimura, Akiko / Hashiguchi, Akihiro / Matsuura, Eiji / Takashima, Hiroshi

    Neurogenetics

    2024  

    Abstract: Biallelic variants of 4-hydroxyphenylpyruvate dioxygenase-like (HPDL) gene have been linked to neurodegenerative disorders ranging from severe neonatal encephalopathy to early-onset spastic paraplegia. We identified a novel homozygous variant, c.340G > T ...

    Abstract Biallelic variants of 4-hydroxyphenylpyruvate dioxygenase-like (HPDL) gene have been linked to neurodegenerative disorders ranging from severe neonatal encephalopathy to early-onset spastic paraplegia. We identified a novel homozygous variant, c.340G > T (p.Gly114Cys), in the HPDL gene in two siblings with autosomal recessive hereditary spastic paraplegia (HSP). Despite sharing the same likely pathogenic variant, the older sister had pure HSP, whereas her brother had severe and complicated HSP, accompanied by early-onset mental retardation and abnormalities in magnetic resonance imaging. Given the clinical heterogeneity and potential for treatable conditions in HPDL-related diseases, we emphasize the importance of genetic testing for the HPDL gene.
    Language English
    Publishing date 2024-01-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1339887-8
    ISSN 1364-6753 ; 1364-6745
    ISSN (online) 1364-6753
    ISSN 1364-6745
    DOI 10.1007/s10048-024-00746-y
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