Article ; Online: Inhibition of chromatin condensation disrupts planar cell migration.
2024 Volume 15, Issue 1, Page(s) 2325961
Abstract: Cell migration involves the actin cytoskeleton, and recently recognized nuclear involvement. In this study, we explore the impact of chromatin remodeling on cell migration using NIH 3T3 cells and a scratch wound assay subjected to pharmacological ... ...
| Abstract | Cell migration involves the actin cytoskeleton, and recently recognized nuclear involvement. In this study, we explore the impact of chromatin remodeling on cell migration using NIH 3T3 cells and a scratch wound assay subjected to pharmacological interventions. We inhibit histone deacetylases (HDACs) with Trichostatin A (TSA) and methyltransferase EZH2 with GSK126 to modulate chromatin compaction. Our results indicate that chromatin modifications impair wound closure efficiency, reduce individual cell migration speed, and disrupt migration persistence. Live-cell imaging reveals dynamic intranuclear chromatin remodeling and nuclear shape parameters during migration, influenced by both small- and large-scale chromatin remodeling. The altered nuclear shape is associated with disrupted cell and nuclear mechanics, suggesting a crucial interplay between chromatin remodeling, nuclear mechanics and migration. These findings shed light on the intricate connection between intranuclear chromatin dynamics, nuclear mechanics, and cell migration, providing a basis for further investigations into the molecular mechanisms governing these processes. |
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| MeSH term(s) | Mice ; Animals ; Chromatin ; Chromatin Assembly and Disassembly ; Cell Movement |
| Chemical Substances | Chromatin |
| Language | English |
| Publishing date | 2024-03-11 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2619626-8 |
| ISSN | 1949-1042 ; 1949-1042 |
| ISSN (online) | 1949-1042 |
| ISSN | 1949-1042 |
| DOI | 10.1080/19491034.2024.2325961 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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