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  1. Article ; Online: Measuring body temperature of freely moving mice under an optogenetics-induced long-term hypothermic state.

    Takahashi, Tohru M / Sakurai, Takeshi / Hirano, Arisa

    STAR protocols

    2023  Volume 4, Issue 2, Page(s) 102321

    Abstract: We present a protocol for inducing a hibernation-like state in free-moving mice using optogenetics. We have recently developed an optogenetic technique utilizing modified Opsin4, which is activated by weak blue light, resulting in prolonged neuronal ... ...

    Abstract We present a protocol for inducing a hibernation-like state in free-moving mice using optogenetics. We have recently developed an optogenetic technique utilizing modified Opsin4, which is activated by weak blue light, resulting in prolonged neuronal excitation. We describe a protocol that includes detailed instructions for virus injection, implantation of optic fibers and temperature transmitters, photostimulation, and real-time recording of body temperature in mice. This method is valuable for investigating the mechanisms underlying torpor and thermoregulation in mice. For complete details on the use and execution of this protocol, please refer to Takahashi et al.
    Language English
    Publishing date 2023-05-31
    Publishing country United States
    Document type Journal Article
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2023.102321
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Analysis of circadian rhythm components in EEG/EMG data of aged mice.

    Masuda, Kosaku / Katsuda, Yoko / Niwa, Yasutaka / Sakurai, Takeshi / Hirano, Arisa

    Frontiers in neuroscience

    2023  Volume 17, Page(s) 1173537

    Abstract: Aging disrupts circadian clocks, as evidenced by a reduction in the amplitude of circadian rhythms. Because the circadian clock strongly influences sleep-wake behavior in mammals, age-related alterations in sleep-wake patterns may be attributable, at ... ...

    Abstract Aging disrupts circadian clocks, as evidenced by a reduction in the amplitude of circadian rhythms. Because the circadian clock strongly influences sleep-wake behavior in mammals, age-related alterations in sleep-wake patterns may be attributable, at least partly, to functional changes in the circadian clock. However, the effect of aging on the circadian characteristics of sleep architecture has not been well assessed, as circadian behaviors are usually evaluated through long-term behavioral recording with wheel-running or infrared sensors. In this study, we examined age-related changes in circadian sleep-wake behavior using circadian components extracted from electroencephalography (EEG) and electromyography (EMG) data. EEG and EMG were recorded from 12 to 17-week-old and 78 to 83-week-old mice for 3 days under light/dark and constant dark conditions. We analyzed time-dependent changes in the duration of sleep. Rapid eye movement (REM) and non-REM (NREM) sleep significantly increased during the night phase in old mice, whereas no significant change was observed during the light phase. The circadian components were then extracted from the EEG data for each sleep-wake stage, revealing that the circadian rhythm in the power of delta waves during NREM sleep was attenuated and delayed in old mice. Furthermore, we used machine learning to evaluate the phase of the circadian rhythm, with EEG data serving as the input and the phase of the sleep-wake rhythm (environmental time) as the output. The results indicated that the output time for the old mice data tended to be delayed, specifically at night. These results indicate that the aging process significantly impacts the circadian rhythm in the EEG power spectrum despite the circadian rhythm in the amounts of sleep and wake attenuated but still remaining in old mice. Moreover, EEG/EMG analysis is useful not only for evaluating sleep-wake stages but also for circadian rhythms in the brain.
    Language English
    Publishing date 2023-05-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2411902-7
    ISSN 1662-453X ; 1662-4548
    ISSN (online) 1662-453X
    ISSN 1662-4548
    DOI 10.3389/fnins.2023.1173537
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Editorial: The Role of Neuropeptides in Sleep/Wakefulness States and the Circadian Clock.

    Tsujino, Natsuko / Ikeda, Masayuki / Hirano, Arisa / Sakurai, Takeshi

    Frontiers in neuroscience

    2022  Volume 16, Page(s) 913371

    Language English
    Publishing date 2022-05-09
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2411902-7
    ISSN 1662-453X ; 1662-4548
    ISSN (online) 1662-453X
    ISSN 1662-4548
    DOI 10.3389/fnins.2022.913371
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  4. Article ; Online: Crucial role of TFAP2B in the nervous system for regulating NREM sleep.

    Nakai, Ayaka / Kashiwagi, Mitsuaki / Fujiyama, Tomoyuki / Iwasaki, Kanako / Hirano, Arisa / Funato, Hiromasa / Yanagisawa, Masashi / Sakurai, Takeshi / Hayashi, Yu

    Molecular brain

    2024  Volume 17, Issue 1, Page(s) 13

    Abstract: The AP-2 transcription factors are crucial for regulating sleep in both vertebrate and invertebrate animals. In mice, loss of function of the transcription factor AP-2β (TFAP2B) reduces non-rapid eye movement (NREM) sleep. When and where TFAP2B functions, ...

    Abstract The AP-2 transcription factors are crucial for regulating sleep in both vertebrate and invertebrate animals. In mice, loss of function of the transcription factor AP-2β (TFAP2B) reduces non-rapid eye movement (NREM) sleep. When and where TFAP2B functions, however, is unclear. Here, we used the Cre-loxP system to generate mice in which Tfap2b was specifically deleted in the nervous system during development and mice in which neuronal Tfap2b was specifically deleted postnatally. Both types of mice exhibited reduced NREM sleep, but the nervous system-specific deletion of Tfap2b resulted in more severe sleep phenotypes accompanied by defective light entrainment of the circadian clock and stereotypic jumping behavior. These findings indicate that TFAP2B in postnatal neurons functions at least partly in sleep regulation and imply that TFAP2B also functions either at earlier stages or in additional cell types within the nervous system.
    MeSH term(s) Animals ; Mice ; Nervous System/metabolism ; Sleep ; Transcription Factor AP-2/genetics ; Transcription Factor AP-2/metabolism ; Transcription Factors
    Chemical Substances Transcription Factor AP-2 ; Transcription Factors ; Tfap2b protein, mouse
    Language English
    Publishing date 2024-02-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2436057-0
    ISSN 1756-6606 ; 1756-6606
    ISSN (online) 1756-6606
    ISSN 1756-6606
    DOI 10.1186/s13041-024-01084-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Singularity response reveals entrainment properties in mammalian circadian clock.

    Masuda, Kosaku / Kon, Naohiro / Iizuka, Kosuke / Fukada, Yoshitaka / Sakurai, Takeshi / Hirano, Arisa

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 2819

    Abstract: Entrainment is characterized by phase response curves (PRCs), which provide a summary of responses to perturbations at each circadian phase. The synchronization of mammalian circadian clocks is accomplished through the receipt of a variety of inputs from ...

    Abstract Entrainment is characterized by phase response curves (PRCs), which provide a summary of responses to perturbations at each circadian phase. The synchronization of mammalian circadian clocks is accomplished through the receipt of a variety of inputs from both internal and external time cues. A comprehensive comparison of PRCs for various stimuli in each tissue is required. Herein, we demonstrate that PRCs in mammalian cells can be characterized using a recently developed estimation method based on singularity response (SR), which represents the response of desynchronized cellular clocks. We confirmed that PRCs can be reconstructed using single SR measurements and quantified response properties for various stimuli in several cell lines. SR analysis reveals that the phase and amplitude after resetting are distinguishable among stimuli. SRs in tissue slice cultures reveal tissue-specific entrainment properties. These results demonstrate that SRs can be employed to unveil entrainment mechanisms with diverse stimuli in multiscale mammalian clocks.
    MeSH term(s) Animals ; Circadian Clocks/physiology ; Circadian Rhythm/physiology ; Time ; Mammals/physiology ; Cues
    Language English
    Publishing date 2023-05-17
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-38392-x
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  6. Article: Aripiprazole disrupts cellular synchrony in the suprachiasmatic nucleus and enhances entrainment to environmental light-dark cycles in mice.

    Li, Ruoshi / Masuda, Kosaku / Ono, Daisuke / Kanbayashi, Takashi / Hirano, Arisa / Sakurai, Takeshi

    Frontiers in neuroscience

    2023  Volume 17, Page(s) 1201137

    Abstract: Many patients with psychiatric conditions, such as bipolar disorder and major depressive disorder, frequently experience disruptions in their sleep-wake cycles. Several case studies and clinical trials have shown that the administration of aripiprazole, ... ...

    Abstract Many patients with psychiatric conditions, such as bipolar disorder and major depressive disorder, frequently experience disruptions in their sleep-wake cycles. Several case studies and clinical trials have shown that the administration of aripiprazole, a commonly prescribed antipsychotic drug, alleviates the symptoms of circadian sleep disorders in these patients. This improvement may be attributed to the effects of aripiprazole on the circadian central clock, specifically the hypothalamic suprachiasmatic nucleus (SCN), which regulates various circadian physiological rhythms, including the sleep-wake cycle, in mammals. To examine whether aripiprazole facilitates adaptation to changes in the light-dark cycle, we orally administered aripiprazole to mice and subjected them to jet-lag experiments. Mice receiving aripiprazole were more rapidly entrained to 6 h advanced light-dark cycles. Moreover, we examined the effect of aripiprazole on the cellular rhythms of SCN slice cultures and found that aripiprazole disrupted cellular synchronization in the SCN, thereby accelerating the damping of the SCN rhythm at the population level. Adenosine 3'5' monophosphate (cAMP) assay using a bioluminescence indicator revealed that intracellular cAMP level in the SCN increased following aripiprazole treatment. However, this increase was blocked by pre-treatment with the serotonin 1A receptor (5-HT
    Language English
    Publishing date 2023-08-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2411902-7
    ISSN 1662-453X ; 1662-4548
    ISSN (online) 1662-453X
    ISSN 1662-4548
    DOI 10.3389/fnins.2023.1201137
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  7. Article ; Online: Structure and Function of Neuronal Circuits Linking Ventrolateral Preoptic Nucleus and Lateral Hypothalamic Area.

    Prokofeva, Kseniia / Saito, Yuki C / Niwa, Yasutaka / Mizuno, Seiya / Takahashi, Satoru / Hirano, Arisa / Sakurai, Takeshi

    The Journal of neuroscience : the official journal of the Society for Neuroscience

    2023  Volume 43, Issue 22, Page(s) 4075–4092

    Abstract: To understand how sleep-wakefulness cycles are regulated, it is essential to disentangle structural and functional relationships between the preoptic area (POA) and lateral hypothalamic area (LHA), since these regions play important yet opposing roles in ...

    Abstract To understand how sleep-wakefulness cycles are regulated, it is essential to disentangle structural and functional relationships between the preoptic area (POA) and lateral hypothalamic area (LHA), since these regions play important yet opposing roles in the sleep-wakefulness regulation. GABA- and galanin (GAL)-producing neurons in the ventrolateral preoptic nucleus (VLPO) of the POA (VLPO
    MeSH term(s) Mice ; Male ; Female ; Animals ; Preoptic Area/physiology ; Hypothalamic Area, Lateral/physiology ; Orexins/metabolism ; Galanin/metabolism ; Neurons/physiology ; Wakefulness/physiology ; Sleep/physiology ; gamma-Aminobutyric Acid/metabolism
    Chemical Substances Orexins ; Galanin (88813-36-9) ; gamma-Aminobutyric Acid (56-12-2)
    Language English
    Publishing date 2023-04-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604637-x
    ISSN 1529-2401 ; 0270-6474
    ISSN (online) 1529-2401
    ISSN 0270-6474
    DOI 10.1523/JNEUROSCI.1913-22.2023
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1668: Show issues Location:
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  8. Article: [Regulation of the circadian rhythm by phosphorylation and ubiquitination].

    Hirano, Arisa / Fukada, Yoshitaka

    Seikagaku. The Journal of Japanese Biochemical Society

    2014  Volume 86, Issue 4, Page(s) 528–531

    MeSH term(s) Animals ; CLOCK Proteins/metabolism ; Circadian Rhythm ; Humans ; Phosphorylation ; Protein Processing, Post-Translational ; Protein Transport ; Ubiquitination
    Chemical Substances CLOCK Proteins (EC 2.3.1.48)
    Language Japanese
    Publishing date 2014-08
    Publishing country Japan
    Document type Journal Article ; Review
    ZDB-ID 282319-6
    ISSN 0037-1017
    ISSN 0037-1017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 592: Show issues Location:
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  9. Article ; Online: Correction: Salt-inducible kinase 3 regulates the mammalian circadian clock by destabilizing PER2 protein.

    Hayasaka, Naoto / Hirano, Arisa / Miyoshi, Yuka / Tokuda, Isao T / Yoshitane, Hikari / Matsuda, Junichiro / Fukada, Yoshitaka

    eLife

    2021  Volume 10

    Language English
    Publishing date 2021-01-25
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.66683
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  10. Article ; Online: The intricate dance of post-translational modifications in the rhythm of life.

    Hirano, Arisa / Fu, Ying-Hui / Ptáček, Louis J

    Nature structural & molecular biology

    2016  Volume 23, Issue 12, Page(s) 1053–1060

    Abstract: Endogenous biological rhythms with approximately 24-h periodicity are generated by the circadian clock, in which clock genes coordinate with one another and form a transcriptional-translational negative feedback loop. The precision of the circadian clock ...

    Abstract Endogenous biological rhythms with approximately 24-h periodicity are generated by the circadian clock, in which clock genes coordinate with one another and form a transcriptional-translational negative feedback loop. The precision of the circadian clock is further regulated by multiple post-translational modifications (PTMs), including phosphorylation, ubiquitination, acetylation and SUMOylation. Here, we review current understanding of the regulatory mechanisms of the core clock proteins by PTMs in the mammalian circadian clock.
    MeSH term(s) Animals ; Circadian Clocks ; Circadian Rhythm ; Circadian Rhythm Signaling Peptides and Proteins/genetics ; Circadian Rhythm Signaling Peptides and Proteins/metabolism ; Humans ; Protein Processing, Post-Translational
    Chemical Substances Circadian Rhythm Signaling Peptides and Proteins
    Language English
    Publishing date 2016-12-06
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2126708-X
    ISSN 1545-9985 ; 1545-9993
    ISSN (online) 1545-9985
    ISSN 1545-9993
    DOI 10.1038/nsmb.3326
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