Article: A whole blood immunoassay using gold nanoshells.
2002 Volume 75, Issue 10, Page(s) 2377–2381
Abstract: A rapid immunoassay capable of detecting analyte within complex biological media without any sample preparation is described. This was accomplished using gold nanoshells, layered dielectric-metal nanoparticles whose optical resonance is a function of the ...
Abstract | A rapid immunoassay capable of detecting analyte within complex biological media without any sample preparation is described. This was accomplished using gold nanoshells, layered dielectric-metal nanoparticles whose optical resonance is a function of the relative size of its constituent layers. Aggregation of antibody/nanoshell conjugates with extinction spectra in the near-infrared was monitored spectroscopically in the presence of analyte. Successful detection of immunoglobulins was achieved in saline, serum, and whole blood. This system constitutes a simple immunoassay capable of detecting sub-nanogram-per-milliliter quantities of various analytes in different media within 10-30 min. |
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MeSH term(s) | Animals ; Biosensing Techniques ; Blood Chemical Analysis/methods ; Gold/chemistry ; Immunoassay/methods ; Immunoglobulins/blood ; Nanotechnology/methods ; Particle Size ; Rabbits ; Spectrophotometry, Ultraviolet |
Chemical Substances | Immunoglobulins ; Gold (7440-57-5) |
Language | English |
Publishing date | 2002-11-26 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. |
ZDB-ID | 1508-8 |
ISSN | 1520-6882 ; 0003-2700 |
ISSN (online) | 1520-6882 |
ISSN | 0003-2700 |
DOI | 10.1021/ac0262210 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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