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Article ; Online: Rapid, label-free pathogen identification system for multidrug-resistant bacterial wound infection detection on military members in the battlefield.

Chen, Ying / Chau, Julie / Yoon, Jung / Hladky, Jeanne

2022 Volume 17, Issue 5, Page(s) e0267945

Abstract: US military service members experiencing combat-related wounds have higher risk of infection by multidrug-resistant bacteria. The gold standard culture-based antimicrobial susceptibility testing (AST) is not feasible in the battlefield environment. Thus, ...

Abstract	US military service members experiencing combat-related wounds have higher risk of infection by multidrug-resistant bacteria. The gold standard culture-based antimicrobial susceptibility testing (AST) is not feasible in the battlefield environment. Thus, a rapid deployable system for bacteria identification and AST directly from wound sample is urgently needed. We report the potential of a Rapid, Label-free Pathogen Identification (RAPID) diagnostic system based on ATR-FTIR method to detect and distinguish multi-drug resistant strains for six different species in the ESKAPEE group. Our RAPID system combines sample processing on-broad to isolate and enrich bacteria cells from wound sample, ATR-FTIR measurement to detect antimicrobial-induced bacterial cell spectral changes, and machine learning model for automated, objective, and quantitative spectral analysis and unknown sample classification. Based on experimental results, our RAPID system is a promising technology for label-free, sensitive (104 cfu/mL from mixture), species-specific (> 95% accuracy), rapid (< 10 min for identification, ~ 4 hours for AST) bacteria detection directly from wound samples.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Infective Agents/pharmacology ; Drug Resistance, Multiple, Bacterial ; Humans ; Microbial Sensitivity Tests ; Military Personnel ; Wound Infection/diagnosis ; Wound Infection/drug therapy
Chemical Substances	Anti-Bacterial Agents ; Anti-Infective Agents
Language	English
Publishing date	2022-05-05
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0267945
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Article: Two Remarks on Graph Norms.

Garbe, Frederik / Hladký, Jan / Lee, Joonkyung

Discrete & computational geometry

2021 Volume 67, Issue 3, Page(s) 919–929

Abstract: ... For a ... ...

Abstract	For a graph
Language	English
Publishing date	2021-02-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	1459007-4
ISSN	1432-0444 ; 0179-5376
ISSN (online)	1432-0444
ISSN	0179-5376
DOI	10.1007/s00454-021-00280-w
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Article ; Online: Calcium has protective impact on cadmium-induced toxicity in lichens.

Kováčik, Jozef / Dresler, Sławomir / Babula, Petr / Hladký, Juraj / Sowa, Ireneusz

Plant physiology and biochemistry : PPB

2020 Volume 156, Page(s) 591–599

Abstract: Eventual protective action of calcium (Ca, 100 or 1000 μM) against cadmium (Cd, 10 or 100 μM) toxicity in common lichen Hypogymnia physodes after 24 h of exposure was studied. Total Cd reached 482 and 2801 μg/g DW in 10 and 100 μM Cd treatments while Ca ... ...

Abstract	Eventual protective action of calcium (Ca, 100 or 1000 μM) against cadmium (Cd, 10 or 100 μM) toxicity in common lichen Hypogymnia physodes after 24 h of exposure was studied. Total Cd reached 482 and 2801 μg/g DW in 10 and 100 μM Cd treatments while Ca content reached over 23 mg/g DW in 1000 μM Ca treatment. Ca suppressed Cd accumulation by 23 and 38% in total fraction and completely in absorbed fraction. Fluorescence microscopy of Cd and Ca ions revealed good correlation with quantitative data. Cd stimulated increase in ROS formation and lipid peroxidation as detected using fluorescent reagents and quantification of H
MeSH term(s)	Antioxidants ; Cadmium/toxicity ; Calcium/pharmacology ; Hydrogen Peroxide ; Lichens/drug effects ; Nitric Oxide ; Parmeliaceae/drug effects
Chemical Substances	Antioxidants ; Cadmium (00BH33GNGH) ; Nitric Oxide (31C4KY9ESH) ; Hydrogen Peroxide (BBX060AN9V) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2020-10-10
Publishing country	France
Document type	Journal Article
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2020.10.007
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Article ; Online: Intra-amniotic infection and sterile intra-amniotic inflammation in women with preterm labor with intact membranes are associated with a higher rate of

Kacerovsky, Marian / Stranik, Jaroslav / Kukla, Rudolf / Bolehovska, Radka / Bostik, Pavel / Matulova, Jana / Stepan, Martin / Hladky, Jan / Jacobsson, Bo / Musilova, Ivana

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians

2021 Volume 35, Issue 25, Page(s) 7344–7352

Abstract: Objective: To determine the prevalence of : Methods: Overall, 115 women with singleton pregnancies complicated by PTL between gestational ages of 22 + 0 and 34 + 6 weeks were included in this study. Paired amniotic and cervical fluid samples were ... ...

Abstract	Objective: To determine the prevalence of Methods: Overall, 115 women with singleton pregnancies complicated by PTL between gestational ages of 22 + 0 and 34 + 6 weeks were included in this study. Paired amniotic and cervical fluid samples were collected at the time of admission via transabdominal amniocentesis using a Dacron polyester swab. Microbial invasion of the amniotic cavity was diagnosed based on a combination of culture and molecular biology methods. Intra-amniotic inflammation was determined based on the concentration of interleukin-6 in the amniotic fluid. Bacterial and Results: Intra-amniotic infection and sterile inflammation were identified in 14% (16/115) and 25% (29/115) of the women, respectively. Conclusions: In PTL , both forms of intra-amniotic inflammation were associated with a higher prevalence of
MeSH term(s)	Pregnancy ; Infant, Newborn ; Female ; Humans ; Infant ; Ureaplasma ; Obstetric Labor, Premature/microbiology ; Amniotic Fluid/microbiology ; Inflammation ; DNA ; Chorioamnionitis/microbiology ; Fetal Membranes, Premature Rupture/microbiology
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2021-07-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2077261-0
ISSN	1476-4954 ; 1057-0802 ; 1476-7058
ISSN (online)	1476-4954
ISSN	1057-0802 ; 1476-7058
DOI	10.1080/14767058.2021.1947231
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Article ; Online: Glyphosate does not show higher phytotoxicity than cadmium: Cross talk and metabolic changes in common herb.

Kováčik, Jozef / Novotný, Vít / Bujdoš, Marek / Dresler, Sławomir / Hladký, Juraj / Babula, Petr

Journal of hazardous materials

2019 Volume 383, Page(s) 121250

Abstract: Toxicity of glyphosate (G) alone or in combination with cadmium (Cd) was studied in Matricaria chamomilla. Cadmium accumulated in shoots and roots in relation to prolonged exposure while glyphosate and aminomethylphosphonic acid (AMPA) were detected only ...

Abstract	Toxicity of glyphosate (G) alone or in combination with cadmium (Cd) was studied in Matricaria chamomilla. Cadmium accumulated in shoots and roots in relation to prolonged exposure while glyphosate and aminomethylphosphonic acid (AMPA) were detected only in roots. After 7 days of exposure, root Cd and G accumulation was similar (56 μg G or 47 μg Cd/g DW in 1 μM treatments and 330 μg G or 321 μg Cd/g DW in 10 μM treatments). Despite this fact, Cd stimulated higher ROS formation and G rather suppressed nitric oxide while H
MeSH term(s)	Antioxidants ; Cadmium/toxicity ; Glycine/analogs & derivatives ; Hydrogen Peroxide ; Organophosphonates ; Oxidative Stress ; Plant Roots ; Glyphosate
Chemical Substances	Antioxidants ; Organophosphonates ; Cadmium (00BH33GNGH) ; aminomethylphosphonic acid (AMPA) (90825O5C1U) ; Hydrogen Peroxide (BBX060AN9V) ; Glycine (TE7660XO1C)
Language	English
Publishing date	2019-09-18
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2019.121250
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Article: Oxidative Stress and Heavy Metals in Plants.

Fryzova, Radka / Pohanka, Miroslav / Martinkova, Pavla / Cihlarova, Hana / Brtnicky, Martin / Hladky, Jan / Kynicky, Jindrich

Reviews of environmental contamination and toxicology

2018 Volume 245, Page(s) 129–156

Abstract: Oxidative stress is a pathological process related to not only animal kingdom but also plants. Regarding oxidative stress in plants, heavy metals are frequently discussed as causative stimuli with relevance to ecology. Because heavy metals have broad ... ...

Abstract	Oxidative stress is a pathological process related to not only animal kingdom but also plants. Regarding oxidative stress in plants, heavy metals are frequently discussed as causative stimuli with relevance to ecology. Because heavy metals have broad technological importance, they can easily contaminate the environment. Much of previous effort regarding the harmful impact of the heavy metals was given to their toxicology in the animals and humans. Their implication in plant pathogeneses is less known and remains underestimated.The current paper summarizes basic facts about heavy metals, their distribution in soil, mobility, accumulation by plants, and initiation of oxidative stress including the decline in basal metabolism. The both actual and frontier studies in the field are summarized and discussed. The major pathophysiological pathways are introduced as well and link between heavy metals toxicity and their ability to initiate an oxidative damage is provided. Mobility and bioaccessibility of the metals is also considered as key factors in their impact on oxidative stress development in the plant. The metals like lead, mercury, copper, cadmium, iron, zinc, nickel, vanadium are depicted in the text.Heavy metals appear to be significant contributors to pathological processes in the plants and oxidative stress is probably an important contributor to the effect. The most sensitive plant species are enlisted and discussed in this review. The facts presented here outline next effort to investigate pathological processes in the plants.
MeSH term(s)	Biological Availability ; Metals, Heavy/metabolism ; Metals, Heavy/toxicity ; Oxidative Stress/drug effects ; Plants/metabolism ; Species Specificity ; Sugars/metabolism
Chemical Substances	Metals, Heavy ; Sugars
Language	English
Publishing date	2018
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	90606-2
ISSN	0179-5953
ISSN	0179-5953
DOI	10.1007/398_2017_7
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Article ; Online: Metabolic responses of Ulva compressa to single and combined heavy metals.

Kováčik, Jozef / Micalizzi, Giuseppe / Dresler, Sławomir / Babula, Petr / Hladký, Juraj / Chemodanov, Alexander / Mondello, Luigi

Chemosphere

2018 Volume 213, Page(s) 384–394

Abstract: Accumulation of metals and metabolic responses were studied for two Cd and Cu concentrations (1 and 10 μM) either alone or as a combination in marine macroalga after 7 days of exposure. Cd accumulated more at a low dose (115 μg of Cd/g DW) but Cu at a ... ...

Abstract	Accumulation of metals and metabolic responses were studied for two Cd and Cu concentrations (1 and 10 μM) either alone or as a combination in marine macroalga after 7 days of exposure. Cd accumulated more at a low dose (115 μg of Cd/g DW) but Cu at a high dose (378 μg of Cu/g DW); Cu suppressed Cd accumulation (by 57%). Na and Zn levels were unaffected, but higher metal doses depleted K and Ca levels. Higher metal concentrations strongly stimulated reactive oxygen species and depleted nitric oxide (NO) formation, but differences between the action of Cd and Cu were not extensive. Higher metal doses increased cell wall thickness with a potential relation to NO signal that is visible mainly in the apoplast in those treatments. A higher Cu dose depleted proline, ascorbic acid, and phenol levels more than Cd, whereas Cd elevated nonprotein thiols and ascorbic acid in combined treatments. An eventual role of malic or citric acid in metal chelation was not evident: malic acid level decreased in all treatments. The total content of fatty acids reached 16.7 mg/g DW in control with the quantitative order of PUFAs > SFAs > MUFAs; palmitic, vaccenic, linoleic, and α-linolenic acids were the major compounds. Cu was more toxic for fatty acids than Cd (even at 1 μM); mainly, PUFA levels strongly decreased (from 43% of total acids in control to 28.9% and 5.4% at 1 and 10 μM Cu treatment, respectively). Results are precisely and critically discussed in relation to limited literature focused on macroalgae, and a comparison with microalgae is also provided.
MeSH term(s)	Chromatography, Gas/methods ; Metals, Heavy/adverse effects ; Metals, Heavy/chemistry ; Microscopy, Fluorescence/methods ; Ulva/drug effects ; Ulva/metabolism
Chemical Substances	Metals, Heavy
Language	English
Publishing date	2018-09-07
Publishing country	England
Document type	Journal Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2018.08.141
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Article ; Online: Nitric oxide affects cadmium-induced changes in the lichen Ramalina farinacea.

Kováčik, Jozef / Dresler, Sławomir / Micalizzi, Giuseppe / Babula, Petr / Hladký, Juraj / Mondello, Luigi

Nitric oxide : biology and chemistry

2018 Volume 83, Page(s) 11–18

Abstract: Metabolic responses of epiphytic lichen Ramalina farinacea to cadmium (Cd) and/or nitric oxide (NO) scavenger (cPTIO) were studied. Accumulation of Cd and other metallic nutrients was not affected by cPTIO while total and absorbed amounts differed. Cd- ... ...

Abstract	Metabolic responses of epiphytic lichen Ramalina farinacea to cadmium (Cd) and/or nitric oxide (NO) scavenger (cPTIO) were studied. Accumulation of Cd and other metallic nutrients was not affected by cPTIO while total and absorbed amounts differed. Cd-induced NO formation was suppressed by cPTIO but ROS signal was synergistically enhanced, confirming that NO is essential to keep ROS under control. This excessive ROS generation could be a reason for depleted amount of all fatty acids, including SFAs, MUFAs and PUFAs. Total content of fatty acids reached 3.89 mg/g DW in control with linoleic (40%), palmitic (24%), oleic (12.8%) and stearic (8%) acids as major compounds: interestingly, shift in relative ratio of saturated (from 40 to 35% of total FAs) versus polyunsaturated fatty acids (from 42 to 48% of total FAs) was observed. Glutathione was suppressed by all treatments but Krebs acids were almost unaffected by cPTIO, indicating no regulatory role of NO in their accumulation. On the contrary, Cd-induced elevation in NO signal was related to increase in ascorbate and proline content while cPTIO suppressed it, indicating a tight relation between NO and these metabolites. Data are compared also with algae and vascular plants to show similarities between various life lineages.
MeSH term(s)	Cadmium/pharmacology ; Free Radical Scavengers/pharmacology ; Lichens/drug effects ; Lichens/metabolism ; Microscopy, Fluorescence ; Nitric Oxide/antagonists & inhibitors ; Nitric Oxide/biosynthesis ; Nitric Oxide/metabolism
Chemical Substances	Free Radical Scavengers ; Cadmium (00BH33GNGH) ; Nitric Oxide (31C4KY9ESH)
Language	English
Publishing date	2018-12-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1362794-6
ISSN	1089-8611 ; 1089-8603
ISSN (online)	1089-8611
ISSN	1089-8603
DOI	10.1016/j.niox.2018.12.001
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Article ; Online: Metabolic changes induced by manganese in chamomile.

Kováčik, Jozef / Dresler, Sławomir / Wójciak-Kosior, Magdalena / Hladký, Juraj / Babula, Petr

Plant physiology and biochemistry : PPB

2018 Volume 133, Page(s) 127–133

Abstract: Manganese (Mn) uptake and toxicity in chamomile (Matricaria chamomilla) and changes of phenolic metabolites in plants grown in the soil (1000 μM ... ...

Abstract	Manganese (Mn) uptake and toxicity in chamomile (Matricaria chamomilla) and changes of phenolic metabolites in plants grown in the soil (1000 μM Mn
MeSH term(s)	Ascorbic Acid/metabolism ; Chamomile/genetics ; Chamomile/metabolism ; Hydroxyl Radical/metabolism ; Lipid Peroxidation/drug effects ; Manganese/pharmacology ; Oxidative Stress/drug effects ; Plant Leaves/genetics ; Plant Leaves/metabolism ; Plant Roots/metabolism ; Plant Shoots/genetics ; Plant Shoots/metabolism ; Sulfhydryl Compounds/metabolism ; Tetraploidy
Chemical Substances	Sulfhydryl Compounds ; Hydroxyl Radical (3352-57-6) ; Manganese (42Z2K6ZL8P) ; Ascorbic Acid (PQ6CK8PD0R)
Language	English
Publishing date	2018-10-29
Publishing country	France
Document type	Journal Article
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2018.10.031
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Book ; Online: Language and Function

Hladký, Josef

To the memory of Jan Firbas

(Studies in Functional and Structural Linguistics ; v.49)

2003

Abstract: The present volume, originally prepared to celebrate Jan Firbas' 80th birthday, unfortunately is presented only belatedly, to commemorate one of the most outstanding personalities of functional and structural linguistics. Its contributors have been ... ...

Series title	Studies in Functional and Structural Linguistics ; v.49
Abstract	The present volume, originally prepared to celebrate Jan Firbas' 80th birthday, unfortunately is presented only belatedly, to commemorate one of the most outstanding personalities of functional and structural linguistics. Its contributors have been inspired by the richness and penetrating invention of Firbas, contained in his analysis of functional sentence perspective and of many other aspects of sentence and discourse
Language	English
Size	Online-Ressource (349 p.)
Publisher	John Benjamins Publishing Company
Publishing place	Amsterdam/Philadelphia
Document type	Book ; Online
Note	Description based upon print version of record
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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