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Article: Metastatic carcinoma of breast in the urinary bladder.

Wong, M H / Yiu, M K / Ho, K L

Hong Kong medical journal = Xianggang yi xue za zhi

2013 Volume 19, Issue 5, Page(s) 455–457

Abstract: Less than 5% of breast cancers present as metastasis, and urinary bladder secondaries were only sporadically reported in the literature. However, they may even be responsible for the initial presentation, for which reason they can pose a diagnostic ... ...

Abstract	Less than 5% of breast cancers present as metastasis, and urinary bladder secondaries were only sporadically reported in the literature. However, they may even be responsible for the initial presentation, for which reason they can pose a diagnostic challenge. We present here what we believe is the first such case in Hong Kong, with a review of this entity.
MeSH term(s)	Aged, 80 and over ; Breast Neoplasms/pathology ; Female ; Hong Kong ; Humans ; Urinary Bladder Neoplasms/diagnosis ; Urinary Bladder Neoplasms/pathology ; Urinary Bladder Neoplasms/secondary
Language	English
Publishing date	2013-10
Publishing country	China
Document type	Case Reports ; Journal Article
ZDB-ID	1239255-8
ISSN	1024-2708
ISSN	1024-2708
DOI	10.12809/hkmj133768
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Article: Bipolar transurethral vapourisation versus monopolar transurethral resection of prostate: a randomised controlled trial.

Ng, C F / Yee, C H / Chan, C K / Wong, H M / Chiu, P Kf / Tsu, J Hl / Teoh, J Yc / Ho, K L

Hong Kong medical journal = Xianggang yi xue za zhi

2018 Volume 23 Suppl 2, Issue 3, Page(s) 32–34

MeSH term(s)	Aged ; Double-Blind Method ; Hong Kong ; Humans ; Laser Therapy/methods ; Length of Stay/statistics & numerical data ; Male ; Middle Aged ; Prospective Studies ; Prostate/surgery ; Prostatic Hyperplasia/surgery ; Quality of Life ; Transurethral Resection of Prostate/methods ; Treatment Outcome
Language	English
Publishing date	2018-06-24
Publishing country	China
Document type	Journal Article ; Multicenter Study ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ZDB-ID	1239255-8
ISSN	1024-2708
ISSN	1024-2708
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Article: Subinguinal microsurgical varicocelectomy for male factor subfertility: ten-year experience.

Leung, L / Ho, K L / Tam, P C / Yiu, M K

Hong Kong medical journal = Xianggang yi xue za zhi

2013 Volume 19, Issue 4, Page(s) 334–340

Abstract: Objective: To investigate the impact of subinguinal microsurgical varicocelectomy on semen parameters and pregnancy outcomes in couples with male factor subfertility.: Design: Case series.: Setting: Male Infertility Clinic in an academic ... ...

Abstract	Objective: To investigate the impact of subinguinal microsurgical varicocelectomy on semen parameters and pregnancy outcomes in couples with male factor subfertility. Design: Case series. Setting: Male Infertility Clinic in an academic institution in Hong Kong. Patients: The clinical records of 42 consecutive subfertile male patients who had subinguinal microsurgical varicocelectomy (from January 2000 to December 2009) were retrospectively reviewed. All the patients had a known history of subfertility and abnormalities in one or more semen parameters. Female subfertility factors were not addressed. Only grade 2 or higher clinically palpable varicoceles were operated on. Main outcome measures: Preoperative and postoperative semen analyses based on the World Health Organization criteria; the outcome measures included changes in semen parameters and whether a pregnancy ensued. Results: The mean age of patients and their spouses were 38 and 33 years, respectively. The mean duration of infertility was 4 years; 37 patients had primary infertility and five had secondary infertility. The mean (± standard deviation) sperm concentration improved from 12 ± 19 million/mL to 23 ± 29 million/mL following varicocelectomy (P<0.001), the mean sperm motility improved from 26% ± 16% to 32% ± 18% (P<0.001), and the mean normal morphology increased from 5% ± 7% to 6% ± 6% (P<0.001). Postoperatively, 23 (55%) of the patients achieved pregnancy, 11 (26%) being spontaneous, 1 (2%) by intrauterine insemination, and 11 (26%) by in-vitro fertilisation. Among 20 patients with severe preoperative oligospermia (<5 million/mL), statistically significant improvements occurred in postoperative mean sperm concentration, motility and morphology (all P<0.001), and five (25%) of them achieved a spontaneous pregnancy. There was one intra-operative injury to the testicular artery with immediate repair and no testicular atrophy. Five (12%) of the patients had recurrences. No preoperative factors appeared predictive of a pregnancy ensuing. Conclusions: In couples with male infertility due to varicoceles, subinguinal microsurgical varicocelectomy was shown to improve sperm concentrations, motility and morphology, and the likelihood of a pregnancy. Spontaneous pregnancy was achieved in 25% of the couples in which the man had severe oligospermia.
MeSH term(s)	Adult ; Female ; Hong Kong ; Humans ; Infertility, Male/etiology ; Infertility, Male/surgery ; Male ; Microsurgery/methods ; Middle Aged ; Oligospermia/etiology ; Oligospermia/surgery ; Pregnancy ; Pregnancy Rate ; Recurrence ; Retrospective Studies ; Sperm Count ; Sperm Motility ; Treatment Outcome ; Varicocele/complications ; Varicocele/surgery ; Young Adult
Language	English
Publishing date	2013-08
Publishing country	China
Document type	Journal Article
ZDB-ID	1239255-8
ISSN	1024-2708
ISSN	1024-2708
DOI	10.12809/hkmj133884
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Article ; Online: Persistent left sided superior vena cava.

Ho, K L / Tan, J L

Heart (British Cardiac Society)

2006 Volume 92, Issue 8, Page(s) 1046

MeSH term(s)	Cardiac Catheterization ; Cardiomyopathy, Dilated ; Catheterization, Central Venous ; Female ; Fluid Therapy/adverse effects ; Humans ; Hypotension/etiology ; Middle Aged ; Resuscitation/adverse effects ; Vena Cava, Superior/abnormalities
Language	English
Publishing date	2006-08
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	1303417-0
ISSN	1468-201X ; 1355-6037
ISSN (online)	1468-201X
ISSN	1355-6037
DOI	10.1136/hrt.2005.077081
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Article: Disease spectrum and treatment patterns in a local male infertility clinic.

Ho, K L / Tsu, James H L / Tam, P C / Yiu, M K

Hong Kong medical journal = Xianggang yi xue za zhi

2015 Volume 21, Issue 1, Page(s) 5–9

Abstract: Objective: To review disease spectrum and treatment patterns in a local male infertility clinic.: Design: Case series.: Setting: Male infertility clinic in a teaching hospital in Hong Kong.: Patients: Patients who were seen as new cases in a ... ...

Abstract	Objective: To review disease spectrum and treatment patterns in a local male infertility clinic. Design: Case series. Setting: Male infertility clinic in a teaching hospital in Hong Kong. Patients: Patients who were seen as new cases in a local male infertility clinic between January 2008 and December 2012. Intervention: Infertility assessment and counselling on treatment options. Main outcome measures: Disease spectrum and treatment patterns. Results: A total of 387 new patients were assessed in the male infertility clinic. The mean age of the patients and their female partners was 37.2 and 32.1 years, respectively. The median duration of infertility was 3 years. Among the patients, 36.2% had azoospermia, 8.0% had congenital absence of vas deferens, and 48.3% of patients had other abnormalities in semen parameters. The commonest causes of male infertility were unknown (idiopathic), clinically significant varicoceles, congenital absence of vas deferens, mumps after puberty, and erectile or ejaculatory dysfunction. Overall, 66.1% of patients chose assisted reproductive treatment and 12.4% of patients preferred surgical correction of reversible male infertility conditions. Altogether 36.7% of patients required either surgical sperm retrieval or correction of male infertility conditions. Conclusions: The present study provided important local data on the disease spectrum and treatment patterns in a male infertility clinic. The incidences of azoospermia and congenital absence of vas deferens were much higher than those reported in the contemporary literature. A significant proportion of patients required either surgical sperm retrieval or correction of reversible male infertility conditions.
MeSH term(s)	Adolescent ; Adult ; Aged ; Ambulatory Care Facilities/statistics & numerical data ; Ejaculation ; Female ; Genital Diseases, Male/complications ; Genital Diseases, Male/epidemiology ; Hong Kong ; Hospitals, Teaching ; Humans ; Infertility, Male/etiology ; Infertility, Male/therapy ; Male ; Male Urogenital Diseases/complications ; Male Urogenital Diseases/epidemiology ; Middle Aged ; Mumps/complications ; Mumps/epidemiology ; Reproductive Techniques, Assisted/statistics & numerical data ; Semen ; Sexual Partners ; Sperm Retrieval/statistics & numerical data ; Vas Deferens/abnormalities ; Vasovasostomy/statistics & numerical data ; Young Adult
Language	English
Publishing date	2015-02
Publishing country	China
Document type	Journal Article
ZDB-ID	1239255-8
ISSN	1024-2708
ISSN	1024-2708
DOI	10.12809/hkmj144376
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Article: Coexistence of primary empty sella and silent corticotrophic adenoma.

Ho, K L

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

1996 Volume 9, Issue 5, Page(s) 521–525

Abstract: Primary empty sellae are rarely associated with an intact pituitary adenoma. Most of such cases are documented clinically and radiographically. This paper reports a case of coexistence of primary empty sella and a silent corticotrophic adenoma in a 61- ... ...

Abstract	Primary empty sellae are rarely associated with an intact pituitary adenoma. Most of such cases are documented clinically and radiographically. This paper reports a case of coexistence of primary empty sella and a silent corticotrophic adenoma in a 61-year-old woman. The en bloc preparation of the sella turcica demonstrates the anatomical relationship of the empty sella and the flattened pituitary gland and the adenoma. The immunostain shows the presence of all five cell types of the adenohypophysis.
MeSH term(s)	Adenoma, Basophil/chemistry ; Adenoma, Basophil/pathology ; Empty Sella Syndrome/metabolism ; Empty Sella Syndrome/pathology ; Female ; Humans ; Immunohistochemistry ; Middle Aged ; Pituitary Neoplasms/chemistry ; Pituitary Neoplasms/pathology ; Sella Turcica/pathology
Language	English
Publishing date	1996-05
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	645073-8
ISSN	1530-0285 ; 0893-3952
ISSN (online)	1530-0285
ISSN	0893-3952
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Zs.A 2450: Show issues

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Article: Electrocardiographical case. Elderly woman with sudden onset of post-operative dyspnoea.

Ho, K L / Teo, W S

Singapore medical journal

2005 Volume 46, Issue 3, Page(s) 144–6; quiz 147

Abstract: A 70-year-old Chinese woman developed breathlessness, tachycardia and hypotension on the fourth day after total hip replacement. 12-lead electrocardiogram (ECG) showed sinus tachycardia with ST depression in I, II, V5 and V6. The ECG changes of sinus ... ...

Abstract	A 70-year-old Chinese woman developed breathlessness, tachycardia and hypotension on the fourth day after total hip replacement. 12-lead electrocardiogram (ECG) showed sinus tachycardia with ST depression in I, II, V5 and V6. The ECG changes of sinus tachycardia along with a typical history is suggestive of pulmonary embolism. Diagnosis, treatment and the use of IVC filter for pulmonary embolism are discussed.
MeSH term(s)	Aged ; Arthroplasty, Replacement, Hip/adverse effects ; Dyspnea/diagnosis ; Electrocardiography ; Female ; Humans ; Postoperative Complications ; Pulmonary Embolism/diagnosis
Language	English
Publishing date	2005-03
Publishing country	Singapore
Document type	Case Reports ; Journal Article
ZDB-ID	604319-7
ISSN	0037-5675
ISSN	0037-5675
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Article ; Online: Delivery of chimeric hepatitis B core particles into liver cells.

Lee, K W / Tey, B T / Ho, K L / Tan, W S

Journal of applied microbiology

2012 Volume 112, Issue 1, Page(s) 119–131

Abstract: Aims: To display a liver-specific ligand on the hepatitis B virus core particles for cell-targeting delivery.: Methods and results: A liver cell-binding ligand (preS1) was fused at the N-terminal end of the hepatitis B core antigen (HBcAg), but the ... ...

Abstract	Aims: To display a liver-specific ligand on the hepatitis B virus core particles for cell-targeting delivery. Methods and results: A liver cell-binding ligand (preS1) was fused at the N-terminal end of the hepatitis B core antigen (HBcAg), but the fusion protein (preS1His(6) HBcAg) was insoluble in Escherichia coli and did not form virus-like particles (VLPs). A method to display the preS1 on the HBcAg particle was established by incorporating an appropriate molar ratio of the truncated HBcAg (tHBcAg) to the preS1His(6) HBcAg. Gold immunomicroscopy showed that the subunit mixture reassembled into icosahedral particles, displaying the preS1 ligand on the surface of VLPs. Fluorescence microscopy revealed that the preS1 ligand delivered the fluorescein-labelled VLPs into the HepG2 cells efficiently. Conclusions: Chimeric VLPs containing the insoluble preS1His(6) HBcAg and highly soluble tHBcAg were produced by a novel incorporation method. The preS1 ligand was exposed on the surface of the VLPs and was shown to deliver fluorescein molecules into liver cells. Significance and impact of study: The newly established incorporation method can be used in the development of chimeric VLPs that could serve as potential nanovehicles to target various cells specifically by substituting the preS1 ligand with different cell-specific ligands.
MeSH term(s)	Escherichia coli/genetics ; Escherichia coli/metabolism ; Genetic Techniques ; HeLa Cells ; Hep G2 Cells ; Hepatitis B Core Antigens/genetics ; Hepatitis B Core Antigens/metabolism ; Hepatocytes/metabolism ; Humans ; Ligands ; Protein Transport ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism
Chemical Substances	Hepatitis B Core Antigens ; Ligands ; Recombinant Fusion Proteins
Language	English
Publishing date	2012-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1358023-1
ISSN	1365-2672 ; 1364-5072
ISSN (online)	1365-2672
ISSN	1364-5072
DOI	10.1111/j.1365-2672.2011.05176.x
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Article: Microsurgical vasoepididymostomy for obstructive azoospermia.

Ho, K L / Wong, M H / Tam, P C

Hong Kong medical journal = Xianggang yi xue za zhi

2009 Volume 15, Issue 6, Page(s) 452–457

Abstract: Objectives: To evaluate the efficacy of microsurgical vasoepididymostomy for patients with obstructive azoospermia attending our institutions.: Design: Retrospective study.: Setting: Division of Urology, Department of Surgery, Queen Mary and Tung ... ...

Abstract	Objectives: To evaluate the efficacy of microsurgical vasoepididymostomy for patients with obstructive azoospermia attending our institutions. Design: Retrospective study. Setting: Division of Urology, Department of Surgery, Queen Mary and Tung Wah hospitals, Hong Kong. Patients: All patients with obstructive azoospermia due to epididymal obstruction who had undergone microsurgical vasoepididymostomy in the study hospitals from July 2001 to November 2007. Main outcome measures: Causes of epididymal obstruction, operative techniques, patency rates, and pregnancy outcomes of their female partners. RESULTS. Twenty-two patients with obstructive azoospermia due to epididymal obstruction had undergone 23 microsurgical vasoepididymostomy procedures. The mean age of patients and their female partners was 36 and 30 years, respectively. Six procedures were performed by the Berger's triangulation intussusception technique and 17 by Marmar or Chan's two-suture intussusception techniques. The mean operating time of unilateral and bilateral procedures was 164 and 203 minutes, respectively. The median follow-up duration was 15 months. The overall patency rate was 57%; being 50% and 64% for unilateral and bilateral procedures, respectively. The patency rate of patients with epididymal fluid positive for sperm was 71%. The mean best sperm count was 23.1 million/mL, with forward motility of 19% and normal morphology of 7%. The overall paternity rate was 32%. Natural pregnancy was achieved in three cases and assisted reproduction was used in four. Conclusions: Microsurgical intussusception vasoepididymostomy is a viable option for couples with male factor infertility due to obstructive azoospermia. Reasonable patency outcomes were achieved in the present series of cases. Individualised counselling, with expectations based on anticipated surgical outcomes, should be offered to couples before resorting to assisted reproduction.
MeSH term(s)	Adult ; Azoospermia/surgery ; Epididymis/surgery ; Female ; Follow-Up Studies ; Humans ; Male ; Microsurgery/methods ; Ostomy/methods ; Pregnancy ; Pregnancy Rate ; Retrospective Studies ; Vas Deferens/surgery
Language	English
Publishing date	2009-12
Publishing country	China
Document type	Evaluation Studies ; Journal Article
ZDB-ID	1239255-8
ISSN	1024-2708
ISSN	1024-2708
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Article: Delivery of chimeric hepatitis B core particles into liver cells

Lee, K.W / Tey, B.T / Ho, K.L / Tan, W.S

Journal of applied microbiology. 2012 Jan., v. 112, no. 1

2012

Abstract: Aims: To display a liver‐specific ligand on the hepatitis B virus core particles for cell‐targeting delivery. Methods and Results: A liver cell–binding ligand (preS1) was fused at the N‐terminal end of the hepatitis B core antigen (HBcAg), but the fusion ...

Abstract	Aims: To display a liver‐specific ligand on the hepatitis B virus core particles for cell‐targeting delivery. Methods and Results: A liver cell–binding ligand (preS1) was fused at the N‐terminal end of the hepatitis B core antigen (HBcAg), but the fusion protein (preS1His6HBcAg) was insoluble in Escherichia coli and did not form virus‐like particles (VLPs). A method to display the preS1 on the HBcAg particle was established by incorporating an appropriate molar ratio of the truncated HBcAg (tHBcAg) to the preS1His6HBcAg. Gold immunomicroscopy showed that the subunit mixture reassembled into icosahedral particles, displaying the preS1 ligand on the surface of VLPs. Fluorescence microscopy revealed that the preS1 ligand delivered the fluorescein‐labelled VLPs into the HepG2 cells efficiently. Conclusions: Chimeric VLPs containing the insoluble preS1His6HBcAg and highly soluble tHBcAg were produced by a novel incorporation method. The preS1 ligand was exposed on the surface of the VLPs and was shown to deliver fluorescein molecules into liver cells. Significance and Impact of Study: The newly established incorporation method can be used in the development of chimeric VLPs that could serve as potential nanovehicles to target various cells specifically by substituting the preS1 ligand with different cell‐specific ligands.
Keywords	Escherichia coli ; Hepatitis B virus ; chimerism ; fluorescein ; fluorescence microscopy ; hepatitis B ; hepatitis B antigens ; hepatocytes ; human cell lines ; liver ; microbiology ; virus-like particles
Language	English
Dates of publication	2012-01
Size	p. 119-131.
Publishing place	Blackwell Publishing Ltd
Document type	Article
ZDB-ID	1358023-1
ISSN	1364-5072
ISSN	1364-5072
DOI	10.1111/j.1365-2672.2011.05176.x
Database	NAL-Catalogue (AGRICOLA)

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