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  1. Article ; Online: New findings on the adaptation of Alternaria solani to major fungicide classes

    Schroeder, Carolina / Hoffmeister, Mascha / Stammler, Gerd

    2023  

    Keywords Text ; abstract_or_summary ; ddc:630
    Language English
    Publisher Julius Kühn-Institut
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Method for QoI sensitivity monitoring in Colletotrichum lentis by a species-specific pyrosequencing assay

    Hoffmeister, Mascha / Martens, Gerald / Yarnell, Lori / Blain, Natalie / Wieja, Birgit / Riediger, Nadine / Stammler, Gerd

    Canadian journal of plant pathology. 2022 Jan. 02, v. 44, no. 1

    2022  

    Abstract: Colletotrichum lentis is an important pathogen of lentils in Canada causing the disease anthracnose. Fungicide applications are an important tool for the control of this disease and, historically, QoI fungicidegdels have been used intensively. Reduced ... ...

    Abstract Colletotrichum lentis is an important pathogen of lentils in Canada causing the disease anthracnose. Fungicide applications are an important tool for the control of this disease and, historically, QoI fungicidegdels have been used intensively. Reduced field efficacy of QoI fungicides on C. lentis has been reported recently and could be linked to the presence of QoI resistant isolates. In this study, the cytochrome b gene of C. lentis was sequenced, and the mutation G143A was identified as the causal resistance mechanism. Furthermore, a sensitive and reliable molecular biological assay was developed for the quantitative detection of the G143A mutation by pyrosequencing in field samples.
    Keywords Colletotrichum ; anthracnose ; bioassays ; cytochrome b ; fungicides ; genes ; high-throughput nucleotide sequencing ; mutation ; pathogens ; plant pathology ; Canada
    Language English
    Dates of publication 2022-0102
    Size p. 128-135.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 763044-x
    ISSN 0706-0661 ; 1715-2992
    ISSN 0706-0661 ; 1715-2992
    DOI 10.1080/07060661.2021.1958010
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Cyp51 Mutationen und ihr Einfluss auf die DMI-Sensitivität von Venturia inaequalis ; Mutations in Cyp51 of Venturia inaequalis and their effects on DMI sensitivity

    Hoffmeister, Mascha / Böhm, Jan / Stammler, Gerd

    2021  

    Keywords Text ; abstract_or_summary ; ddc:630
    Language German
    Publisher Julius Kühn-Institut
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Tracking host infection and reproduction of Peronospora salviae‐officinalis using an improved method for confocal laser scanning microscopy (CSLM)

    Hoffmeister, Mascha / Maier, Wolfgang / Thines, Marco / Becker, Yvonne

    2020  

    Abstract: Peronospora salviae-officinalis, the causal agent of downy mildew on common sage, is an obligate biotrophic pathogen. It grows in the intercellular spaces of the leaf tissue of sage and forms intracellular haustoria to interface with host cells. Although ...

    Abstract Peronospora salviae-officinalis, the causal agent of downy mildew on common sage, is an obligate biotrophic pathogen. It grows in the intercellular spaces of the leaf tissue of sage and forms intracellular haustoria to interface with host cells. Although P. salviae-officinalis was described as a species of its own 10 years ago, the infection process remains obscure. To address this, a histological study of various infection events, from the adhesion of conidia on the leaf surface to de novo sporulation is presented here. As histological studies of oomycetes are challenging due to the lack of chitin in their cell wall, we also present an improved method for staining downy mildews for confocal laser scanning microscopy as well as evaluating the potential of autofluorescence of fixed nonstained samples. For staining, a 1:1 mixture of aniline blue and trypan blue was found most suitable and was used for staining of oomycete and plant structures, allowing discrimination between them as well as the visualization of plant immune responses. The method was also used to examine samples of Peronospora lamii on Lamium purpureum and Peronospora belbahrii on Ocimum basilicum, demonstrating the potential of the presented histological method for studying the infection processes of downy mildews in general.
    Keywords Text ; ddc:630 ; aniline blue ; confocal laser scanning microscopy ; Peronospora belbahrii ; Peronospora lamii ; Peronospora salviae-officinalis ; trypan blue
    Subject code 630
    Language English
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Epidemiology of sage downy mildew, Peronospora salviae-officinalis

    Hoffmeister, Mascha / Gabriel, Doreen / Thines, Marco / Maier, Wolfgang

    2020  

    Abstract: Downy mildew of common sage (Salvia officinalis), caused by Peronospora salviae-officinalis, has become a serious problem in sage production worldwide. The effect of temperature was determined for conidia germination and disease development. In vitro, ... ...

    Abstract Downy mildew of common sage (Salvia officinalis), caused by Peronospora salviae-officinalis, has become a serious problem in sage production worldwide. The effect of temperature was determined for conidia germination and disease development. In vitro, conidial germination rate was highest at temperatures between 10 °C and 20 °C and was strongly reduced at temperatures above 25, but conidia were also able to germinate at 2 °C. Temperatures between 15 and 20 °C were most favourable for infection and disease progress in infection experiments in climate chambers, with highest sporulating leaf area observed at 15 and 20 °C, and highest symptomatic leaf area at 20 °C. P. salviae-officinalis is still able to infect sage plants at 5 °C, but sporulation was only observed at higher temperatures. Oospores developed 14 days after inoculation at 15 °C, and 8 days after inoculation at 20 and 25 °C. The infection trials also showed that dark incubation is not a prerequisite for successful infection of sage. Furthermore, P. salviae-officinalis needs two events of leaf wetness or high humidity to complete its asexual life cycle. First, a leaf wetness event of at least three hours is needed for conidial germination and penetration of the host. Second, high humidity of at least 90% is needed at the end of the infection cycle for sporulation. Seed washing revealed the presence of P. salviae-officinalis oospores in seed lots of sage suggesting that infested seeds might play a major role in the fast spread of sage downy mildew, which is an important finding for phytosanitary or quarantine measures.
    Keywords Text ; ddc:630 ; Downy mildew ; Infection studies ; Oospores ; Peronospora salviae-officinalis ; Salvia officinalis ; Seed testing
    Subject code 630 ; 580
    Language English
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Two new species of the Peronospora belbahrii species complex, Pe. choii sp. nov. and Pe. salviae-pratensis sp. nov., and a new host for Pe. salviae-officinalis

    Hoffmeister, Mascha / Ashrafi, Samad / Thines, Marco / Maier, Wolfgang

    2020  

    Abstract: The downy mildew species parasitic to Mentheae are of particular interest, as this tribe of Lamiaceae contains a variety of important medicinal plants and culinary herbs. Over the past two decades, two pathogens, Peronospora belbahrii and Pe. salviae- ... ...

    Abstract The downy mildew species parasitic to Mentheae are of particular interest, as this tribe of Lamiaceae contains a variety of important medicinal plants and culinary herbs. Over the past two decades, two pathogens, Peronospora belbahrii and Pe. salviae-officinalis have spread globally, impacting basil and common sage production, respectively. In the original circumscription of Pe. belbahrii, the downy mildew of coleus (Plectranthus scutellarioides) was ascribed to this species in the broader sense, but subtle differences in morphological and molecular phylogenetic analyses using two genes suggested that this pathogen would potentially need to be assigned to a species of its own. In the present study, Peronospora species causing downy mildew on members of the Mentheae, including clary sage (Salvia sclarea), meadow sage (S. pratensis), basil (Ocimum basilicum), ground ivy (Glechoma hederacea) and coleus (Plectranthus scutellarioides) were studied using light microscopy and molecular phylogenetic analyses based on six loci (ITS rDNA, cox1, cox2, ef1a, hsp90 and β-tubulin) to clarify the species boundaries in the Pe. belbahrii species complex. The downy mildew on Salvia pratensis is shown to be distinct from Pe. salviae-officinalis and closely related to Pe. glechomae, and is herein described as a new species, Pe. salviae-pratensis. The downy mildew on S. sclarea was found to be caused by Pe. salviae-officinalis. This is of phytopathological importance, because meadow sage thus does not play a role as inoculum source for common sage in the natural habitat of the former in Europe and Asia, while clary sage probably does. The multi-gene phylogeny revealed that the causal agent of downy mildew on coleus is distinct from Pe. belbahrii on basil, and is herein described as a new taxon, Pe. choii.
    Keywords Text ; ddc:630 ; New taxa ; Downy mildew ; Peronosporaceae ; Coleus ; Multi-gene Phylogeny ; New Host Report
    Subject code 590 ; 580
    Language English
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Peronospora salviae-officinalis - neue Erkenntnisse zu Infektionsbiologie, Epidemiologie und Wirtsspektrum

    Hoffmeister, Mascha / Becker, Yvonne / Maier, Wolfgang

    2020  

    Keywords Text ; abstract_or_summary ; ddc:630
    Language German
    Publisher Verein für Arznei- und Gewürzpflanzen SALUPLANTA e. V.
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Book ; Audio / Video ; Online: Entwicklung neuer Strategien zur Abwehr pilzlicher Schadorganismen in der Echten Salbei (Salvia officinalis)

    Hoffmeister, Mascha / Maier, Wolfgang

    2017  

    Keywords Text ; ddc:630
    Language German
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Schaffung effizienter Strategien zur Kontrolle von Falschem Mehltau (Peronospora salviae-officinalis) und anderen Schadpilzen an der Echten Salbei

    Hoffmeister, Mascha / Maier, Wolfgang

    2017  

    Keywords Text ; abstract_or_summary ; ddc:630
    Language German
    Publisher Verein für Arznei- und Gewürzpflanzen SALUPLANTA e. V.
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Book ; Audio / Video ; Online: Establishment of efficient strategies for controlling downy mildew (Peronospora salviae-officinalis) and other pathogenic fungi on common sage (Salvia officinalis)

    Hoffmeister, Mascha / Maier, Wolfgang

    2016  

    Keywords ddc:630
    Language English
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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