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  1. Article ; Online: Designing and Assembling Plasmids for the Construction of Escherichia coli Biosensor for Vibrio cholerae Detection.

    Holowko, Maciej B / Poh, Chueh Loo

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1772, Page(s) 445–456

    Abstract: In the process of constructing and characterizing the whole cell biosensor for Vibrio cholerae detection, two main techniques have been employed-DNA assembly using the Gibson isothermal assembly reaction was used for the assembly of the PCRed plasmid ... ...

    Abstract In the process of constructing and characterizing the whole cell biosensor for Vibrio cholerae detection, two main techniques have been employed-DNA assembly using the Gibson isothermal assembly reaction was used for the assembly of the PCRed plasmid fragments (DNA parts), and microplate fluorescence readings were used for bacterial strain characterization. The general workflow can be summed up as: the in silico designed DNA fragments were assembled by isothermal assembly to be later transformed into Escherichia coli that, in turn, was characterized using the microplate reader. As fine-tuning of the sensor design was required, the process was repeated iteratively until the final strain was created with desired characteristics. This chapter describes in detail this workflow for different constructs which finally led to the creation of the first whole cell biosensor in E. coli for V. cholerae detection.
    MeSH term(s) Biosensing Techniques/methods ; DNA/genetics ; Escherichia coli/genetics ; Plasmids/genetics ; Vibrio cholerae/genetics
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2018-05-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7795-6_26
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Building a biofoundry.

    Holowko, Maciej B / Frow, Emma K / Reid, Janet C / Rourke, Michelle / Vickers, Claudia E

    Synthetic biology (Oxford, England)

    2020  Volume 6, Issue 1, Page(s) ysaa026

    Abstract: A biofoundry provides automation and analytics infrastructure to support the engineering of biological systems. It allows scientists to perform synthetic biology and aligned experimentation on a high-throughput scale, massively increasing the solution ... ...

    Abstract A biofoundry provides automation and analytics infrastructure to support the engineering of biological systems. It allows scientists to perform synthetic biology and aligned experimentation on a high-throughput scale, massively increasing the solution space that can be examined for any given problem or question. However, establishing a biofoundry is a challenging undertaking, with numerous technical and operational considerations that must be addressed. Using collated learnings, here we outline several considerations that should be addressed prior to and during establishment. These include drivers for establishment, institutional models, funding and revenue models, personnel, hardware and software, data management, interoperability, client engagement and biosecurity issues. The high cost of establishment and operation means that developing a long-term business model for biofoundry sustainability in the context of funding frameworks, actual and potential client base, and costing structure is critical. Moreover, since biofoundries are leading a conceptual shift in experimental design for bioengineering, sustained outreach and engagement with the research community are needed to grow the client base. Recognition of the significant, long-term financial investment required and an understanding of the complexities of operationalization is critical for a sustainable biofoundry venture. To ensure state-of-the-art technology is integrated into planning, extensive engagement with existing facilities and community groups, such as the Global Biofoundries Alliance, is recommended.
    Language English
    Publishing date 2020-12-16
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2397-7000
    ISSN (online) 2397-7000
    DOI 10.1093/synbio/ysaa026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli.

    Holowko, Maciej B / Wang, Huijuan / Jayaraman, Premkumar / Poh, Chueh Loo

    ACS synthetic biology

    2016  Volume 5, Issue 11, Page(s) 1275–1283

    Abstract: Cholera is a potentially mortal, infectious disease caused by Vibrio cholerae bacterium. Current treatment methods of cholera still have limitations. Beneficial microbes that could sense and kill the V. cholerae could offer potential alternative to ... ...

    Abstract Cholera is a potentially mortal, infectious disease caused by Vibrio cholerae bacterium. Current treatment methods of cholera still have limitations. Beneficial microbes that could sense and kill the V. cholerae could offer potential alternative to preventing and treating cholera. However, such V. cholerae targeting microbe is still not available. This microbe requires a sensing system to be able to detect the presence of V. cholera bacterium. To this end, we designed and created a synthetic genetic sensing system using nonpathogenic Escherichia coli as the host. To achieve the system, we have moved proteins used by V. cholerae for quorum sensing into E. coli. These sensor proteins have been further layered with a genetic inverter based on CRISPRi technology. Our design process was aided by computer models simulating in vivo behavior of the system. Our sensor shows high sensitivity to presence of V. cholerae supernatant with tight control of expression of output GFP protein.
    Language English
    Publishing date 2016-11-18
    Publishing country United States
    Document type Journal Article
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.6b00079
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Repurposing a Two-Component System-Based Biosensor for the Killing of Vibrio cholerae.

    Jayaraman, Premkumar / Holowko, Maciej B / Yeoh, Jing Wui / Lim, Sierin / Poh, Chueh Loo

    ACS synthetic biology

    2017  Volume 6, Issue 7, Page(s) 1403–1415

    Abstract: New strategies to control cholera are urgently needed. This study develops an in vitro proof-of-concept sense-and-kill system in a wild-type Escherichia coli strain to target the causative pathogen Vibrio cholerae using a synthetic biology approach. Our ... ...

    Abstract New strategies to control cholera are urgently needed. This study develops an in vitro proof-of-concept sense-and-kill system in a wild-type Escherichia coli strain to target the causative pathogen Vibrio cholerae using a synthetic biology approach. Our engineered E. coli specifically detects V. cholerae via its quorum-sensing molecule CAI-1 and responds by expressing the lysis protein YebF-Art-085, thereby self-lysing to release the killing protein Art-085 to kill V. cholerae. For this report, we individually characterized YebF-Art-085 and Art-085 expression and their activities when coupled to our previously developed V. cholerae biosensing circuit. We show that, in the presence of V. cholerae supernatant, the final integrated sense-and-kill system in our engineered E. coli can effectively inhibit the growth of V. cholerae cells. This work represents the first step toward a novel probiotic treatment modality that could potentially prevent and treat cholera in the future.
    Language English
    Publishing date 2017-07-21
    Publishing country United States
    Document type Journal Article
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.7b00058
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: A model-driven approach towards rational microbial bioprocess optimization.

    Yeoh, Jing Wui / Jayaraman, Sudhaghar S/O / Tan, Sean Guo-Dong / Jayaraman, Premkumar / Holowko, Maciej B / Zhang, Jingyun / Kang, Chang-Wei / Leo, Hwa Liang / Poh, Chueh Loo

    Biotechnology and bioengineering

    2020  Volume 118, Issue 1, Page(s) 305–318

    Abstract: Due to sustainability concerns, bio-based production capitalizing on microbes as cell factories is in demand to synthesize valuable products. Nevertheless, the nonhomogenous variations of the extracellular environment in bioprocesses often challenge the ... ...

    Abstract Due to sustainability concerns, bio-based production capitalizing on microbes as cell factories is in demand to synthesize valuable products. Nevertheless, the nonhomogenous variations of the extracellular environment in bioprocesses often challenge the biomass growth and the bioproduction yield. To enable a more rational bioprocess optimization, we have established a model-driven approach that systematically integrates experiments with modeling, executed from flask to bioreactor scale, and using ferulic acid to vanillin bioconversion as a case study. The impacts of mass transfer and aeration on the biomass growth and bioproduction performances were examined using minimal small-scale experiments. An integrated model coupling the cell factory kinetics with the three-dimensional computational hydrodynamics of bioreactor was developed to better capture the spatiotemporal distributions of bioproduction. Full-factorial predictions were then performed to identify the desired operating conditions. A bioconversion yield of 94% was achieved, which is one of the highest for recombinant Escherichia coli using ferulic acid as the precursor.
    MeSH term(s) Benzaldehydes/metabolism ; Biomass ; Bioreactors ; Coumaric Acids/metabolism ; Escherichia coli/genetics ; Escherichia coli/growth & development
    Chemical Substances Benzaldehydes ; Coumaric Acids ; ferulic acid (AVM951ZWST) ; vanillin (CHI530446X)
    Language English
    Publishing date 2020-10-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 280318-5
    ISSN 1097-0290 ; 0006-3592
    ISSN (online) 1097-0290
    ISSN 0006-3592
    DOI 10.1002/bit.27571
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Author Correction: Building a global alliance of biofoundries.

    Hillson, Nathan / Caddick, Mark / Cai, Yizhi / Carrasco, Jose A / Chang, Matthew Wook / Curach, Natalie C / Bell, David J / Feuvre, Rosalind Le / Friedman, Douglas C / Fu, Xiongfei / Gold, Nicholas D / Herrgård, Markus J / Holowko, Maciej B / Johnson, James R / Johnson, Richard A / Keasling, Jay D / Kitney, Richard I / Kondo, Akihiko / Liu, Chenli /
    Martin, Vincent J J / Menolascina, Filippo / Ogino, Chiaki / Patron, Nicola J / Pavan, Marilene / Poh, Chueh Loo / Pretorius, Isak S / Rosser, Susan J / Scrutton, Nigel S / Storch, Marko / Tekotte, Hille / Travnik, Evelyn / Vickers, Claudia E / Yew, Wen Shan / Yuan, Yingjin / Zhao, Huimin / Freemont, Paul S

    Nature communications

    2019  Volume 10, Issue 1, Page(s) 3132

    Abstract: The original version of this Comment contained errors in the legend of Figure 2, in which the locations of the fifteenth and sixteenth GBA members were incorrectly given as '(15) Australian Genome Foundry, Macquarie University; (16) Australian Foundry ... ...

    Abstract The original version of this Comment contained errors in the legend of Figure 2, in which the locations of the fifteenth and sixteenth GBA members were incorrectly given as '(15) Australian Genome Foundry, Macquarie University; (16) Australian Foundry for Advanced Biomanufacturing, University of Queensland.'. The correct version replaces this with '(15) Australian Foundry for Advanced Biomanufacturing (AusFAB), University of Queensland and (16) Australian Genome Foundry, Macquarie University'. This has been corrected in both the PDF and HTML versions of the Comment.
    Language English
    Publishing date 2019-07-11
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-019-10862-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Building a global alliance of biofoundries.

    Hillson, Nathan / Caddick, Mark / Cai, Yizhi / Carrasco, Jose A / Chang, Matthew Wook / Curach, Natalie C / Bell, David J / Le Feuvre, Rosalind / Friedman, Douglas C / Fu, Xiongfei / Gold, Nicholas D / Herrgård, Markus J / Holowko, Maciej B / Johnson, James R / Johnson, Richard A / Keasling, Jay D / Kitney, Richard I / Kondo, Akihiko / Liu, Chenli /
    Martin, Vincent J J / Menolascina, Filippo / Ogino, Chiaki / Patron, Nicola J / Pavan, Marilene / Poh, Chueh Loo / Pretorius, Isak S / Rosser, Susan J / Scrutton, Nigel S / Storch, Marko / Tekotte, Hille / Travnik, Evelyn / Vickers, Claudia E / Yew, Wen Shan / Yuan, Yingjin / Zhao, Huimin / Freemont, Paul S

    Nature communications

    2019  Volume 10, Issue 1, Page(s) 2040

    MeSH term(s) Biomedical Research/methods ; Biotechnology/instrumentation ; Genetic Engineering ; International Cooperation ; Organisms, Genetically Modified
    Language English
    Publishing date 2019-05-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-019-10079-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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