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Article ; Online: Antibacterial Effect of Cell-Free Supernatant from Lactobacillus pentosus L-36 against Staphylococcus aureus from Bovine Mastitis

Gengchen Wang / Hong Zeng

Molecules, Vol 27, Iss 7627, p

2022 Volume 7627

Abstract: This study sought to analyze the main antibacterial active components of Lactobacillus pentosus ( L. pentosus ) L-36 cell-free culture supernatants (CFCS) in inhibiting the growth of Staphylococcus aureus ( S. aureus ), to explore its physicochemical ... ...

Abstract	This study sought to analyze the main antibacterial active components of Lactobacillus pentosus ( L. pentosus ) L-36 cell-free culture supernatants (CFCS) in inhibiting the growth of Staphylococcus aureus ( S. aureus ), to explore its physicochemical properties and anti-bacterial mechanism. Firstly, the main antibacterial active substance in L-36 CFCS was peptides, which inferred by adjusting pH and enzyme treatment methods. Secondly, the physicochemical properties of the antibacterial active substances in L-36 CFCS were studied from heat, pH, and metal ions, respectively. It demonstrated good antibacterial activity when heated at 65 °C, 85 °C and 100 °C for 10 and 30 min, indicating that it had strong thermal stability. L-36 CFCS had antibacterial activity when the pH value was 2–6, and the antibacterial active substances became stable with the decrease in pH value. After 10 kinds of metal ions were treated, the antibacterial activity did not change significantly, indicating that it was insensitive to metal ions. Finally, scanning electron microscopy, transmission electron microscopy and fluorescence probe were used to reveal the antibacterial mechanism of S. aureus from the aspects of cell morphology and subcellular structure. The results demonstrated that L-36 CFCS could form 1.4–2.3 nm pores in the cell membrane of S. aureus , which increased the permeability of the bacterial cell membrane, resulting in the depolarization of cell membrane potential and leakage of nucleic acid protein and other cell contents. Meanwhile, a large number of ROS are produced and accumulated in the cells, causing damage to DNA, and with the increase in L-36 CFCS concentration, the effect is enhanced, and finally leads to the death of S. aureus . Our study suggests that the main antibacterial active substances of L-36 CFCS are peptides. L-36 CFCS are thermostable, active under acidic conditions, insensitive to metal ions, and exhibit antibacterial effects by damaging cell membranes, DNA and increasing ROS. Using lactic acid ...
Keywords	antibacterial activity ; anti-bacterial mechanism ; Lactobacillus pentosus ; Staphylococcus aureus ; biofilm ; Organic chemistry ; QD241-441
Subject code	540
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Online Learning Self-Efficacy as a Mediator between the Instructional Interactions and Achievement Emotions of Rural Students in Elite Universities

Li-Hong Zeng / Yungwei Hao / Kai-Hsin Tai

Sustainability, Vol 14, Iss 7231, p

2022 Volume 7231

Abstract: Previous research has shown that social capital (teachers’ and peers’ interaction) is a challenge for rural students in China’s elite universities due to underlying issues of online learning self-efficacy (OLSE) and the quality of interaction. To ... ...

Abstract	Previous research has shown that social capital (teachers’ and peers’ interaction) is a challenge for rural students in China’s elite universities due to underlying issues of online learning self-efficacy (OLSE) and the quality of interaction. To understand how interaction quality is influenced, the present study drew on the achievement emotion theory to explore the mediating role of OLSE between social interactions (teacher–student, student–student) and achievement emotions (enjoyment, hopelessness, shame). Data were collected using an online questionnaire with a sample of rural students studying at elite universities ( n = 479) in China. The results analyzed through Structural Equation Modeling confirmed the mediation model in which self-efficacy is a mediator in the relationships between social interactions and three types of achievement emotion as participants learned online during the coronavirus disease 2019 (COVID-19) lockdown.
Keywords	online learning ; self-efficacy ; social capital ; social interactions ; COVID-19 ; achievement emotions ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
Subject code	370
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Cooperative and Non-Cooperative Green Advertising in the Low-Carbon Supply Chain under Monopoly or Competitive Market

Hong Zeng / Dongqin Jiang / Yimeng Li

Sustainability, Vol 14, Iss 15, p

2022 Volume 9190

Abstract: Customers feel a degree of ambiguity associated with low-carbon products, such as remanufactured products, and undervalue them. Although green advertising significantly impacts consumers’ acceptance of remanufactured products and the low-carbon supply ... ...

Abstract	Customers feel a degree of ambiguity associated with low-carbon products, such as remanufactured products, and undervalue them. Although green advertising significantly impacts consumers’ acceptance of remanufactured products and the low-carbon supply chain (LCSC), limited research has been carried out on advertisement decisions for remanufactured products in the LCSC. This study introduces a two-echelon remanufacturing supply chain motivated by the practice of applying green advertising to update consumers’ low-carbon awareness. We use a game-theoretical approach to analyze the remanufacturer’s and retailer’s decisions on the green advertising program (non-coop vs. co-op green advertising) under different competitive scenarios in the LCSC. We find that the LCSC’s and the retailer’s profits are optimal in the non-coop green advertising under the monopoly, but the LCSC’s and the remanufacturer’s profits are optimal in the co-op green advertising under the competition. We suggest that the entry and royalty fees can be applied to coordinate the LCSC under different competitive scenarios.
Keywords	cooperative green advertising ; the low-carbon supply chain ; coordination ; competition ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
Subject code	380
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Bridging substrate intake kinetics and bacterial growth phenotypes with flux balance analysis incorporating proteome allocation

Hong Zeng / Aidong Yang

Scientific Reports, Vol 10, Iss 1, Pp 1-

2020 Volume 10

Abstract: Abstract Empirical kinetic models such as the Monod equation have been widely applied to relate the cell growth with substrate availability. The Monod equation shares a similar form with the mechanistically-based Michaelis-Menten kinetics for enzymatic ... ...

Abstract	Abstract Empirical kinetic models such as the Monod equation have been widely applied to relate the cell growth with substrate availability. The Monod equation shares a similar form with the mechanistically-based Michaelis-Menten kinetics for enzymatic processes, which has provoked long-standing and un-concluded conjectures on their relationship. In this work, we integrated proteome allocation principles into a Flux Balance Analysis (FBA) model of Escherichia coli, which quantitatively revealed potential mechanisms that underpin the phenomenological Monod parameters: the maximum specific growth rate could be dictated by the abundance of growth-controlling proteome and growth-pertinent proteome cost; more importantly, the Monod constant (K s ) was shown to relate to the Michaelis constant for substrate transport (K m,g ), with the link being dependent on the cell’s metabolic strategy. Besides, the proposed model was able to predict glucose uptake rate at given external glucose concentration through the size of available proteome resource for substrate transport and its enzymatic cost, while growth rate and acetate overflow were accurately simulated for two E. coli strains. Bridging the enzymatic kinetics of substrate intake and overall growth phenotypes, this work offers a mechanistic interpretation to the empirical Monod law, and demonstrates the potential of coupling local and global cellular constrains in predictive modelling.
Keywords	Medicine ; R ; Science ; Q
Subject code	660
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: General Construction of Asymmetric Amine Ethers via Efficient Transesterification.

Li, Ziwei / Hong, Zeng / Qian, Chao / Chen, Xinzhi / Zhou, Shaodong

Chemistry (Weinheim an der Bergstrasse, Germany)

2023 Volume 30, Issue 10, Page(s) e202303832

Abstract: A novel method to prepare asymmetric amine ethers is reported. Tertiary amine alcohol hydrogen sulfate intermediates are prepared through a reactive distillation process, followed by the transesterification process to afford eventually asymmetric amine ... ...

Abstract	A novel method to prepare asymmetric amine ethers is reported. Tertiary amine alcohol hydrogen sulfate intermediates are prepared through a reactive distillation process, followed by the transesterification process to afford eventually asymmetric amine ethers. Experiments and DFT calculations revealed the essential roles the sulfate group plays in the highly selective monoesterification process. This clean method is tolerant towards various functional groups with good yields under mild condition, which is obviously superior compared to the conventional processes.
Language	English
Publishing date	2023-12-21
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1478547-X
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.202303832
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Underlying Mechanisms of Reductive Amination on Pd-Catalysts: The Unique Role of Hydroxyl Group in Generating Sterically Hindered Amine.

Hong, Zeng / Ge, Xin / Zhou, Shaodong

International journal of molecular sciences

2022 Volume 23, Issue 14

Abstract: Pd nanospecies supported on porous g-C3N4 nanosheets were prepared for efficient reductive amination reactions. The structures of the catalysts were characterized via FTIR, XRD, XPS, SEM, TEM, and TG analysis, and the mechanisms were investigated using ... ...

Abstract	Pd nanospecies supported on porous g-C3N4 nanosheets were prepared for efficient reductive amination reactions. The structures of the catalysts were characterized via FTIR, XRD, XPS, SEM, TEM, and TG analysis, and the mechanisms were investigated using in situ ATR−FTIR spectroscopic analysis complemented by theoretical calculation. It transpired that the valence state of the Pd is not the dominating factor; rather, the hydroxyl group of the Pd(OH)2 cluster is crucial. Thus, by passing protons between different molecules, the hydroxyl group facilitates both the generation of the imine intermediate and the reduction of the C=N unit. As a result, the sterically hindered amines can be obtained at high selectivity (>90%) at room temperature.
MeSH term(s)	Amination ; Amines/chemistry ; Catalysis ; Imines
Chemical Substances	Amines ; Imines
Language	English
Publishing date	2022-07-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23147621
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Finger Movement Recognition via High-Density Electromyography of Intrinsic and Extrinsic Hand Muscles

Xuhui Hu / Aiguo Song / Jianzhi Wang / Hong Zeng / Wentao Wei

Scientific Data, Vol 9, Iss 1, Pp 1-

2022 Volume 12

Abstract: Measurement(s) Electromyography Technology Type(s) Surface Electrode Factor Type(s) hand gesture Sample Characteristic - Organism Homo sapiens Sample Characteristic - Environment human house Sample Characteristic - Location ... ...

Abstract	Measurement(s) Electromyography Technology Type(s) Surface Electrode Factor Type(s) hand gesture Sample Characteristic - Organism Homo sapiens Sample Characteristic - Environment human house Sample Characteristic - Location China
Keywords	Science ; Q
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Fanconi anemia complementation group D2 promotes sensitivity of endometrial cancer cells to chemotherapeutic agents by inhibiting the ferroptosis pathway

Hai-Hong Lin / Wei-Hong Zeng / Hai-Kun Yang / Li-Shan Huang / Ru Pan / Nan-Xiang Lei

BMC Women's Health, Vol 24, Iss 1, Pp 1-

2024 Volume 10

Abstract: Abstract Background Resistance can develop during treatment of advanced endometrial cancer (EC), leading to unsatisfactory results. Fanconi anemia complementation group D2 (Fancd2) has been shown to be closely related to drug resistance in cancer cells. ... ...

Abstract	Abstract Background Resistance can develop during treatment of advanced endometrial cancer (EC), leading to unsatisfactory results. Fanconi anemia complementation group D2 (Fancd2) has been shown to be closely related to drug resistance in cancer cells. Therefore, this study was designed to explore the correlation of Fancd2 with EC resistance and the mechanism of Fancd2. Methods Real-time quantitative PCR (RT-qPCR) was used to detect the expression of Fancd2 in EC tissues and cells. EC cells (Ishikawa) and paclitaxel-resistant EC cells (Ishikawa/TAX) were transfected to knock down Fancd2. In addition, the ferroptosis inhibitor Ferrostatin-1 was adopted to treat Ishikawa/TAX cells. The sensitivity of cancer cells to chemotherapeutic agents was observed via 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, and inhibitory concentration (IC)50 was calculated. Reactive oxygen species (ROS) levels were measured by flow cytometry, the activity of malondialdehyde (MDA) and the levels of glutathione (GSH) and Fe2+ in cells were detected by corresponding kits, and protein expression of solute farrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) was obtained through western blot. Results Compared with the normal tissues and endometrial epithelial cells, Fancd2 expression was significantly increased in EC tissues and Ishikawa cells, respectively. After knock-down of Fancd2, Ishikawa cells showed significantly increased sensitivity to chemotherapeutic agents. Besides, compared with Ishikawa cells, the levels of ROS, the activity of MDA, and the levels of GSH and Fe2+ were significantly decreased in Ishikawa/TAX cells, while the expression levels of SLC7A11 and GPX4 were significantly increased. Knock-down of Fancd2 significantly increased the ferroptosis levels in Ishikawa/TAX cells, but this effect could be reversed by Ferrostatin-1. Conclusion Fancd2 increases drug resistance in EC cells by inhibiting the cellular ferroptosis pathway.
Keywords	Fancd2 ; Endometrial cancer ; Chemotherapeutic agents ; Drug resistance ; Ferroptosis ; Gynecology and obstetrics ; RG1-991 ; Public aspects of medicine ; RA1-1270
Subject code	616
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: An algorithm for cognitive fusion targeted tumor puncture based on 3-D mathematical modelling

Yong Luo / Junjie Ren / Jun Long / Li Wang / Hong Zeng / Dali Tong

Heliyon, Vol 9, Iss 1, Pp e12742- (2023)

2023

Abstract: Background: Percutaneous puncture is an important means of tumor diagnosis and treatment. At present, most puncture operations are still based on imaging location and clinical experience, and quantitative and accurate targeted puncture cannot be achieved. ...

Abstract	Background: Percutaneous puncture is an important means of tumor diagnosis and treatment. At present, most puncture operations are still based on imaging location and clinical experience, and quantitative and accurate targeted puncture cannot be achieved. How to improve the accuracy of percutaneous tumor puncture, avoid errors to the greatest extent, reduce the occurrence of complications, and improve the overall clinical diagnosis and treatment quality and curative effect, are scientific problems worthy of further study. Method: In the present study, mathematical modeling was first used to construct the tumor puncture path, determine the needle entry angle, and define the relevant limited parameters and the substitution formula. Secondly, relevant parameters were extracted from CT and other imaging data and substituted into formulas, the deviation angle and puncture path were determined, and the personalized tumor puncture scheme was carried out. Third, targeted puncture was precisely implemented under the guidance of B-ultrasound. Compared with the traditional empirical puncture, our model improved the accuracy, decreased the puncture time, and reduced the pain of diagnosis and treatment for patients. Results: A tumor-targeted puncture model was established based on mathematical theory and imaging data. By extracting clinical data, such as tumor radius, projection distance of tumor center and projection distance from puncture point to body surface, the optimal puncture deviation angle was modeled and calculated and a personalized puncture scheme was established. Compared with the conventional method, our model markedly increased the puncture accuracy rate by ∼30%. The puncture number was decreased by ∼50% using our model. Furthermore, our model shortened the operation time by 20% to ease pain of patients and guarantee greater security for patients. Doctor satisfaction and patient discomfort scores were examined. Our model improved doctor satisfaction by ∼20% and reduced subjective discomfort of patients by ...
Keywords	Precise percutaneous tumor puncture ; Radiological data ; Space analytic geometry ; Mathematical modelling ; Algorithm ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	616
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: A methodology for applying the counterflow flame extinction propensity to the flame extinction determination in fire simulations

Yu, Hong-Zeng / Ren, Ning

Fire safety journal. 2020 Apr. 28,

2020

Abstract: A methodology is presented herein for the determination of flame extinction in fire simulations by utilizing the flame extinction propensity obtained from the counterflow flame experiments or calculations. The methodology leverages the fact that the ... ...

Abstract	A methodology is presented herein for the determination of flame extinction in fire simulations by utilizing the flame extinction propensity obtained from the counterflow flame experiments or calculations. The methodology leverages the fact that the flame extinction in hydrocarbon fires tends to occur at critical temperatures corresponding to the strain rates acting on the flames, under the condition that the flame extinction is caused only by physical effects such as dilution and heat extraction. The methodology calls for the correlations between the adiabatic flame extinction temperature and strain rate, a functional expression of the characteristic eddy strain rate in the buoyancy-induced turbulent flow, and the determination of local adiabatic flame temperatures in the fire. The methodology was evaluated by implementing the above application elements in FireFOAM, and comparing the resulted simulations to a previously published study on propane fire intensity in a nitrogen-diluted air environment. The evaluation is deemed favorable for the methodology to be used as an alternative for the flame extinction determination in fire simulations.
Keywords	air ; deformation ; fire intensity ; fires ; heat ; propane ; temperature ; turbulent flow
Language	English
Dates of publication	2020-0428
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	1483569-1
ISSN	0379-7112
ISSN	0379-7112
DOI	10.1016/j.firesaf.2020.103100
Database	NAL-Catalogue (AGRICOLA)

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