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  1. AU="Hongo, Akane"
  2. AU="Krykorková, I"
  3. AU=Yan Bing
  4. AU="Nakos, Konstantinos"
  5. AU="Schreiner, Ryan"
  6. AU=Pltz T
  7. AU="Akhmanova, Anna" AU="Akhmanova, Anna"
  8. AU="Goretsky, Anton"
  9. AU="Cordoza, Makayla L"
  10. AU=Midoux Patrick AU=Midoux Patrick
  11. AU="Mundt, H M"
  12. AU=Tsivitse Susan

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  1. Artikel ; Online: Occurrence of distinctive cells and effects of irradiance on vascular formation in leaves of shade-tolerant C

    Hongo, Akane / Abe, Hinako / Yabiku, Takayuki / Ueno, Osamu

    Journal of plant research

    2023  Band 136, Heft 5, Seite(n) 691–704

    Abstract: The denser leaf vasculature of ... ...

    Abstract The denser leaf vasculature of C
    Sprache Englisch
    Erscheinungsdatum 2023-06-27
    Erscheinungsland Japan
    Dokumenttyp Journal Article
    ZDB-ID 2077362-6
    ISSN 1618-0860 ; 0918-9440
    ISSN (online) 1618-0860
    ISSN 0918-9440
    DOI 10.1007/s10265-023-01475-3
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Development of Cell Analysis Software for Cultivated Corneal Endothelial Cells.

    Okumura, Naoki / Ishida, Naoya / Kakutani, Kazuya / Hongo, Akane / Hiwa, Satoru / Hiroyasu, Tomoyuki / Koizumi, Noriko

    Cornea

    2017  Band 36, Heft 11, Seite(n) 1387–1394

    Abstract: Purpose: To develop analysis software for cultured human corneal endothelial cells (HCECs).: Methods: Software was designed to recognize cell borders and to provide parameters such as cell density, coefficient of variation, and polygonality of ... ...

    Abstract Purpose: To develop analysis software for cultured human corneal endothelial cells (HCECs).
    Methods: Software was designed to recognize cell borders and to provide parameters such as cell density, coefficient of variation, and polygonality of cultured HCECs based on phase contrast images. Cultured HCECs with high or low cell density were incubated with Ca-free and Mg-free phosphate-buffered saline for 10 minutes to reveal the cell borders and were then analyzed with software (n = 50).
    Results: Phase contrast images showed that cell borders were not distinctly outlined, but these borders became more distinctly outlined after phosphate-buffered saline treatment and were recognized by cell analysis software. The cell density value provided by software was similar to that obtained using manual cell counting by an experienced researcher. Morphometric parameters, such as the coefficient of variation and polygonality, were also produced by software, and these values were significantly correlated with cell density (Pearson correlation coefficients -0.62 and 0.63, respectively).
    Conclusions: The software described here provides morphometric information from phase contrast images, and it enables subjective and noninvasive quality assessment for tissue engineering therapy of the corneal endothelium.
    Sprache Englisch
    Erscheinungsdatum 2017-11
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 604826-2
    ISSN 1536-4798 ; 0277-3740
    ISSN (online) 1536-4798
    ISSN 0277-3740
    DOI 10.1097/ICO.0000000000001317
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 1790: Hefte anzeigen Standort:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Artikel ; Online: The Effect of a p38 Mitogen-Activated Protein Kinase Inhibitor on Cellular Senescence of Cultivated Human Corneal Endothelial Cells.

    Hongo, Akane / Okumura, Naoki / Nakahara, Makiko / Kay, EunDuck P / Koizumi, Noriko

    Investigative ophthalmology & visual science

    2017  Band 58, Heft 9, Seite(n) 3325–3334

    Abstract: Purpose: We have begun a clinical trial of a cell-based therapy for corneal endothelial dysfunction in Japan. The purpose of this study was to investigate the usefulness of a p38 MAPK inhibitor for prevention cellular senescence in cultivated human ... ...

    Abstract Purpose: We have begun a clinical trial of a cell-based therapy for corneal endothelial dysfunction in Japan. The purpose of this study was to investigate the usefulness of a p38 MAPK inhibitor for prevention cellular senescence in cultivated human corneal endothelial cells (HCECs).
    Methods: HCECs of 10 donor corneas were divided and cultured with or without SB203580 (a p38 MAPK inhibitor). Cell density and morphology were evaluated by phase-contrast microscopy. Expression of function-related proteins was examined by immunofluorescent staining. Cellular senescence was evaluated by SA-β-gal staining and Western blotting for p16 and p21. Senescence-associated factors were evaluated by membrane blotting array, quantitative PCR, and ELISA.
    Results: Phase-contrast microscopy showed a significantly higher cell density for HCECs cultured with SB203580 than without SB203580 (2623 ± 657 cells/mm2 and 1752 ± 628 cells/mm2, respectively). The HCECs cultured with SB203580 maintained a hexagonal morphology and expressed ZO-1, N-cadherin, and Na+/K+-ATPase in the plasma membrane, whereas the control HCECs showed an altered staining pattern for these marker proteins. HCECs cultured without SB203580 showed high positive SA-β-gal staining, a low nuclear/cytoplasm ratio, and expression of p16 and p21. IL-6, IL-8, CCL2, and CXCL1 were observed at high levels in low cell density HCECs cultured without SB203580.
    Conclusions: Activation of p38 MAPK signaling due to culture stress might be a causative factor that induces cellular senescence; therefore, the use of p38 MAPK inhibitor to counteract senescence may achieve sufficient numbers of HCECs for tissue engineering therapy for corneal endothelial dysfunction.
    Mesh-Begriff(e) Biomarkers/metabolism ; Cadherins/metabolism ; Cells, Cultured ; Cellular Senescence/drug effects ; Cellular Senescence/physiology ; Chemokines/metabolism ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Endothelium, Corneal/cytology ; Humans ; Imidazoles/pharmacology ; Interleukins/metabolism ; Protein Kinase Inhibitors/pharmacology ; Protein-Serine-Threonine Kinases/metabolism ; Pyridines/pharmacology ; Sodium-Potassium-Exchanging ATPase/metabolism ; Zonula Occludens-1 Protein/metabolism ; p38 Mitogen-Activated Protein Kinases/physiology
    Chemische Substanzen Biomarkers ; Cadherins ; Chemokines ; Imidazoles ; Interleukins ; Protein Kinase Inhibitors ; Pyridines ; TJP1 protein, human ; Zonula Occludens-1 Protein ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; p38 Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; Sodium-Potassium-Exchanging ATPase (EC 3.6.3.9) ; SB 203580 (OU13V1EYWQ)
    Sprache Englisch
    Erscheinungsdatum 2017-07-01
    Erscheinungsland United States
    Dokumenttyp Clinical Trial ; Journal Article
    ZDB-ID 391794-0
    ISSN 1552-5783 ; 0146-0404
    ISSN (online) 1552-5783
    ISSN 0146-0404
    DOI 10.1167/iovs.16-21170
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 45: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

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