Article ; Online: Prunus Armeniaca L
Anti-cancer agents in medicinal chemistry
2020 Volume 21, Issue 5, Page(s) 621–629
Abstract: Background: Despite significant advances in therapeutic interventions, liver cancer is the leading cause of cancer mortality in the world. Potential phytochemicals have shown to be promising agents against many life-threatening diseases because of their ...
| Abstract | Background: Despite significant advances in therapeutic interventions, liver cancer is the leading cause of cancer mortality in the world. Potential phytochemicals have shown to be promising agents against many life-threatening diseases because of their low toxicity and potential effectiveness. Objective: The current study aims to conduct an in vitro investigation of the anticancer activity of Apricot Extract (AE) and Amygdalin Containing Fraction (ACF), additionally studying their therapeutic effects on DMBAinduced liver carcinogenesis mice model to highlight their related biochemical and molecular mechanisms. Methods and results: Amygdalin was isolated from the seeds of P. armeniaca L. Male mice received AE or ACF, DMBA, DMBA+AE, DMBA+ACF, and vehicles. The oxidative stress and antioxidant markers, cell proliferation by flow cytometric analysis of Proliferating Cell Nuclear Antigen (PCNA) expression, angiogenesis marker (VEGF), inflammatory marker (TNF-α), apoptotic, anti-apoptotic and autophagy genes expression (caspase-3, Bcl-2, and Beclin-1) were investigated. AE and ACF were found to stimulate the apoptotic process by up-regulating caspase-3 expression and down-regulating Bcl-2 expression. They also reduced VEGF and PCNA levels and increased the antioxidant defense system. Moreover, AE and ACF treatments also inhibited HepG2 and EAC cell proliferation and up-regulated Beclin-1 expression. Conclusion: This study provides evidence that, in DMBA-induced hepatocarcinogenesis, the key proteins involved in the proliferation, angiogenesis, autophagy, and apoptosis are feasible molecular targets for hepatotherapeutic potential using AE and ACF. |
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| MeSH term(s) | Amygdalin/chemical synthesis ; Amygdalin/chemistry ; Amygdalin/pharmacology ; Animals ; Antineoplastic Agents, Phytogenic/chemistry ; Antineoplastic Agents, Phytogenic/isolation & purification ; Antineoplastic Agents, Phytogenic/pharmacology ; Apoptosis/drug effects ; Cell Proliferation/drug effects ; Dose-Response Relationship, Drug ; Drug Screening Assays, Antitumor ; Hep G2 Cells ; Humans ; Liver Neoplasms/drug therapy ; Liver Neoplasms/metabolism ; Liver Neoplasms/pathology ; Liver Neoplasms, Experimental/drug therapy ; Liver Neoplasms, Experimental/metabolism ; Liver Neoplasms, Experimental/pathology ; Male ; Mice ; Mitochondria/drug effects ; Mitochondria/metabolism ; Molecular Structure ; Plant Extracts/chemistry ; Plant Extracts/isolation & purification ; Plant Extracts/pharmacology ; Prunus armeniaca/chemistry ; Seeds/chemistry ; Structure-Activity Relationship |
| Chemical Substances | Antineoplastic Agents, Phytogenic ; Plant Extracts ; Amygdalin (214UUQ9N0H) |
| Language | English |
| Publishing date | 2020-06-03 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 2217610-X |
| ISSN | 1875-5992 ; 1871-5206 |
| ISSN (online) | 1875-5992 |
| ISSN | 1871-5206 |
| DOI | 10.2174/1871520620666200608124003 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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