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Article ; Online: In vitro evaluation of antibacterial effect of combination of honey and Aloe vera extract against Enterococcus faecalis and Escherichia coli

Paria Motahari / Hossein Samadi Kafil / Zahra Taheri Bonab

Advances in Bioscience and Clinical Medicine, Vol 9, Iss 1, Pp 4-

2021 Volume 7

Abstract: Antimicrobial agents are used as intra-canal drugs or canal cleaners for complete dental root canal cleaning. This new study attempts to compare the antibacterial effect of 2% chlorhexidine and combination of honey and Aloe vera extract (H-Av mixture) ... ...

Abstract	Antimicrobial agents are used as intra-canal drugs or canal cleaners for complete dental root canal cleaning. This new study attempts to compare the antibacterial effect of 2% chlorhexidine and combination of honey and Aloe vera extract (H-Av mixture) against Enterococcus faecalis (E.faecalis) and Escherichia coli (E.coli) which are the most important pathogens isolated from infectious root canals. Enterococcus faecalis (ATCC® 29212™) and Escherichia coli (ATCC® 25922™) were cultured in the tryptic soy broth medium. Dilution of H-Av mixture by the method of macrodilution to determine Minimum Inhibitory Concentration was investigated. In this paper agar well diffusion and colony count methods were also used to ensure the accuracy of the results. The results were statistically analyzed by student’s t-test. The significant level established at 5% (P<0.05). The results of the methods of macrodilution, colony count and Agar well diffusion confirm the inhibitory effect of H-Av mixture on E.coli and E. fecalis. Statistically, there was no significant difference between the antibacterial effect of 2% chlorhexidine and 25% H-Av mixture (P>0.05) Increasing drug resistance to antimicrobial compounds needs to study of new drugs against pathogens. H-Av mixture with benefits such as availability, good taste and easier use than chlorhexidine, and fewer side effects can be a good option for intracanal irrigation after clinical trials.
Keywords	antibacterial ; honey ; aloe vera extract ; enterococcus faecalis ; escherichia coli ; Medicine ; R ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Australian International Academic Centre PTY.LTD.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Prevalence of methicillin-resistant Staphylococcus aureus colonization in the emergency department health care workers

Seyed pouya Paknezhad / Kavous Shahsavarinia / Hossein Samadi Kafil / parisa nayeri

Journal of Research in Clinical Medicine, Vol 8, Iss 1, Pp 30-

2020 Volume 30

Abstract: Introduction: AThe aim of this study was to evaluate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization in the health care workers of emergency department of Imam Reza hospital, Tabriz, Iran Methods: In a total of 63 ... ...

Abstract	Introduction: AThe aim of this study was to evaluate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization in the health care workers of emergency department of Imam Reza hospital, Tabriz, Iran Methods: In a total of 63 physicians and nurses in the emergency department of Imam Reza hospital, after obtaining written consent and completing a checklist, a sample of nasal mucosa was collected for microbiological examination and isolation of Staphylococcus aureus (SA) strain. Determination of resistance patterns (by diffusion method) was performed according to the standard methods of methicillin. Results: Out of 63 participants, 8 subjects (12.7%) had colonization of S. aureus; 4 of them were physicians (17.3% of the participated physicians) and 4 were nurses (10% of the participated nurses). Out of 8 isolated SA, 2 cases (25%) and overall 3.1% were methicillin-resistant. In our study, there was a significant relationship between co-employment in other hospitals and patient care at home with SA colonization, but there was not a significant relationship with specialty, work experience, sinusitis, history of antibiotic use, history of skin disease, hand washing habits and use of personal protective equipment. Conclusion: Prevalence of MRSA was not high among health workers of emergency department.
Keywords	staphylococcus aureus ; methicillin resistance ; prevalence ; Medicine (General) ; R5-920
Subject code	360
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences Press
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Postbiotics as a Safe Alternative to Live Probiotic Bacteria in the Food and Pharmaceutical Industries

aziz Homayouni Rad / Leili Aghebati Maleki / Hossein Samadi Kafil / Amin Abbasi / Nader Khani

مجله علمی دانشگاه علوم پزشکی کردستان, Vol 26, Iss 4, Pp 132-

2021 Volume 157

Abstract: Background and Aim: Food is the fundamental need of human life and has nutrients that support growth and be healthy. Gastrointestinal tract (GI) microbiota involves beneficial microorganisms that develop therapeutic effects and are characterize as ... ...

Abstract	Background and Aim: Food is the fundamental need of human life and has nutrients that support growth and be healthy. Gastrointestinal tract (GI) microbiota involves beneficial microorganisms that develop therapeutic effects and are characterize as probiotics. The studies on appropriate probiotic strains have led to the separation and description of specific metabolic byproducts of probiotics named postbiotics. Materials and Methods: A literature review has been performing using PubMed, Medline, and Scopus databases using combinations of search terms “probiotic, postbiotic, immune system, bioactivity, cancer, health-promoting, food, and pharmaceutical industry”. All experimental studies were enrolling for this explanatory review. Articles that did not have a full text were excluded. Results: The probiotics must maintain their survival against inappropriate growth conditions of the processing, storage, distribution, preparation, and digestion system so that they can play their most health effects. Conversely, probiotic metabolites have successfully overcome these unfavorable conditions and maybe a good alternative to probiotics. Due to their specific chemical structure, safe profile, long shelf-life, and the fact that they contain various signaling molecules, postbiotics may have anti-inflammatory, immunomodulatory, antihypertensive, inhibiting abnormal cell proliferation, and antioxidant activities. Conclusions: Postbiotics is mimic the fundamental and clinical role of probiotics, and due to their unique characteristics, can be applied in a delivery system (pharmaceutical/functional foods) to achieve health-promoting, prevention, and treatment purposes.
Keywords	postbiotic ; probiotic ; functional foods ; immune system ; health-promotion ; Medicine ; R ; Medicine (General) ; R5-920
Language	Persian
Publishing date	2021-10-01T00:00:00Z
Publisher	Kurdistan University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Microbial Surface Treatment of Sand with Sporosarcina pasteurii to Improve the Wind Erosion Resistance in Urmia Lake

Hooshang Katebi / Ahmad Fahmi / Ahad Ouria / Alireza Babaeian Amini / Hossein Samadi Kafil

Applied and Environmental Soil Science, Vol

2021 Volume 2021

Abstract: Background. In this research, the potential of the microbially induced carbonate precipitation method for the surface treatment of sand samples of Jabal Kandi dunes, located in the adjacency of Urmia Lake in the northwest of Iran, was considered. Methods. ...

Abstract	Background. In this research, the potential of the microbially induced carbonate precipitation method for the surface treatment of sand samples of Jabal Kandi dunes, located in the adjacency of Urmia Lake in the northwest of Iran, was considered. Methods. Sporosarcina pasteurii was used as ureolytic bacteria for the preparation of the microbial solution. Corn steep liquor containing urea was used as an inexpensive growth media. The effects of the concentration of cementation solution and the number of treatment cycles were considered. Because of the presence of some hydrolyzed urea in the prepared microbial suspension, two methods of treatment, i.e., the mixed and separate addition of microbial solution and cementation solution to the sand surface, were investigated. Penetration and erosion resistance of the microbial treated sand (sand crust) were measured using a handheld penetrometer and a wind tunnel system. Results. The results showed that the penetration and erosion resistance of the treated sand samples via microbial-induced carbonate precipitation method were improved significantly. In the method with the separate addition of microbial and cementation solutions to the sand surface, a stable sand crust was created on top of the sand. Discussion. This study tried to optimize the microbial application of Sporosarcina pasteurii for surface treatment of sand via microbial-induced carbonate precipitation. Significant and results showed that this method can be used on the field scale for the stabilization of sand dunes. The advanced biotechnology application of this bacterium can be used as an environmentally friendly and safe method instead of other methods.
Keywords	Agriculture (General) ; S1-972 ; Environmental sciences ; GE1-350
Subject code	630
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: COVID‐19 reinfection in a healthcare worker after exposure with high dose of virus

Shahram Ahmadian / Hadis Fathizadeh / Saeid Shabestari Khiabani / Mohammad Asgharzadeh / Hossein Samadi Kafil

Clinical Case Reports, Vol 9, Iss 6, Pp n/a-n/a (2021)

A case report

2021

Abstract: Abstract Reinfection with COVID‐19 is possible after exposure to a high dose of the virus. Due to immunity acquired during the previous infection, light symptoms are expected. The finding indicates importance of continuous protection in healthcare ... ...

Abstract	Abstract Reinfection with COVID‐19 is possible after exposure to a high dose of the virus. Due to immunity acquired during the previous infection, light symptoms are expected. The finding indicates importance of continuous protection in healthcare workers.
Keywords	case report ; COVID‐19 ; pandemic ; reinfection ; SARS‐CoV‐2 ; Medicine ; R ; Medicine (General) ; R5-920
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	Wiley
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Lactobacilli species diversity in gut microbiota of renal failure patients

Gholamreza Hanifi / Hossein Samadi Kafil / Hamid Tayebi Khosroshahi / Reza Shapouri / Mohammad Asgharzadeh

Journal of King Saud University: Science, Vol 32, Iss 4, Pp 2365-

2020 Volume 2369

Abstract: Lactobacillus species and other acid producing bacteria could inhibit aerobic bacteria, normalize the intestinal microbiota and decrease the uremic toxins in patients with chronic kidney disease. Therefore, the aim of this study is assessment of ... ...

Abstract	Lactobacillus species and other acid producing bacteria could inhibit aerobic bacteria, normalize the intestinal microbiota and decrease the uremic toxins in patients with chronic kidney disease. Therefore, the aim of this study is assessment of abundance of lactobacillus spp. in fecal flora of End-stage kidney disease (ESKD) and kidney transplanted patients. DNA of 20 fecal samples of ESKD and kidney-transplanted as patients group and 20 fecal samples of non-ESKD and -kidney-transplanted as control group, which were admitted to hospitals, were extracted. Amplified DNA by 16srRNA illumina V3 and V4 primers were sequenced by MiSeq system. Total 651 strains, 37 (5.68%) strains were identified as order Lactobacillales. The strains were classified into six family, nine genus and 37 species. The most abundance genus in both groups are Streptococcus spp. and Lactobacillus spp. and the lowest abundance genus in both groups are Pediococcus and Leuconostoc. Comparing the abundance mean of the strains revealed that there is no significant association between control group and disease group, but the abundance mean of the strains in control group increase in compared to disease group. More diversity was exhibited in lactobacillus spp. in patients with chronic kidney disease compared to control group. Some of the species of Lactobacillaceae family such as L. acidophilus was not found in both patients and control groups. In addition, more of the species of Lactobacillaceae were decreased in patients group, whereas certain species were increased in patients group compared to control group.
Keywords	Lactobacillus ; Chronic kidney disease ; Renal failure patient ; End-stage kidney disease (ESKD) ; Fecal flora ; Science (General) ; Q1-390
Subject code	610
Language	English
Publishing date	2020-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Immunological Aspects of Dental Implant Rejection

Milad Baseri / Faraz Radmand / Reyhaneh Hamedi / Mehdi Yousefi / Hossein Samadi Kafil

BioMed Research International, Vol

2020 Volume 2020

Abstract: Nowadays, dental implants are a prominent therapeutic approach among dentists for replacing missing teeth. Failure in dental implants is a severe challenge recently. The factors which lead to dental implant failure are known. These factors can be ... ...

Abstract	Nowadays, dental implants are a prominent therapeutic approach among dentists for replacing missing teeth. Failure in dental implants is a severe challenge recently. The factors which lead to dental implant failure are known. These factors can be categorized into different groups. In this article, we discussed the immunological aspects of implant failure as one of these groups. Cytokines and immune cells have extensive and various functions in peri-implantitis. The equilibrium between pro and anti-inflammatory cytokines and cells, which involve in this orchestra, has a crucial role in implant prognosis. In conclusion, immune cells, especially macrophages and dendritic cells, almost increased in the patients with implant failure. Also, proinflammatory cytokines were proposed as diagnostic factors according to their higher levels in dental implant rejection.
Keywords	Medicine ; R
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Synthesis, Crystal Structure, and Biological Activity of a Multidentate Calix[4]arene Ligand Doubly Functionalized by 2-Hydroxybenzeledene-Thiosemicarbazone

Ehsan Bahojb Noruzi / Behrouz Shaabani / Silvano Geremia / Neal Hickey / Patrizia Nitti / Hossein Samadi Kafil

Molecules, Vol 25, Iss 2, p

2020 Volume 370

Abstract: The design and synthesis of a novel tert-butyl-calix[4]arene functionalized at 1, 3 positions of the lower rim with two terminal 2-hydroxybenzeledene-thiosemicarbazone moieties is reported. The new ligand with multi-dentate chelating properties was fully ...

Abstract	The design and synthesis of a novel tert-butyl-calix[4]arene functionalized at 1, 3 positions of the lower rim with two terminal 2-hydroxybenzeledene-thiosemicarbazone moieties is reported. The new ligand with multi-dentate chelating properties was fully characterized by several techniques: ESI-Mass spectroscopy, FT-IR, 1H-NMR, and single crystal X-ray diffraction. The solid state structure confirms that the calix[4]arene macrocycle has the expected open cone conformation, with two opposite phenyl rings inclined outwards with large angles. The conformation of the two alkoxythiosemicarbazone arms produces a molecule with a C2 point group symmetry. An interesting chiral helicity is observed, with the two thiosemicarbazone groups oriented in opposite directions like a two-blade propeller. A water molecule is encapsulated in the center of the two-blade propeller through multiple H-bond coordinations. The antibacterial, antifungal, anticancer, and cytotoxic activities of the calix[4]arene-thiosemicarbazone ligand and its metal derivatives (Co2+, Ni2+, Cu2+, and Zn2+) were investigated. A considerable antibacterial activity (in particular against E. coli , MIC, and MBC = 31.25 μg/mL) was observed for the ligand and its metal derivatives. Significant antifungal activities against yeast ( C. albicans ) were also observed for the ligand (MIC = 31.25 μg/mL and MBC = 125 μg/mL) and for its Co 2+ derivative (MIC = 62.5 μg/mL). All compounds show cytotoxicity against the tested cancerous cells. For the Saos-2 cell line, the promising anticancer activity of ligand L (IC 50 < 25 μg/mL) is higher than its metal derivatives. The microscopic analysis of DAPI-stained cells shows that the treated cells change in morphology, with deformation and fragmentation of the nuclei. The hemo-compatibility study demonstrated that this class of compounds are suitable candidates for further in vivo investigations.
Keywords	thiosemicarbazone ; calix[4]arene ; metal complex ; x-ray structure ; antimicrobial ; anticancer ; Organic chemistry ; QD241-441
Subject code	540
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Postbiotics as novel health-promoting ingredients in functional foods

Aziz Homayouni Rad / Leili Aghebati Maleki / Hossein Samadi Kafil / Hamideh Fathi Zavoshti / Aamin Abbasi

Health Promotion Perspectives, Vol 10, Iss 1, Pp 3-

2020 Volume 4

Keywords	Nutrition. Foods and food supply ; TX341-641 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Exosomes from Adipose Tissue-derived Mesenchymal Stem Cells Induce Regulatory T Cells in COVID‐19 Patients

Morteza Motallebnezhad / Ali Hazrati / Hadi Esmaeili Gouvarchin Ghaleh / Nematollah Jonaidi-Jafari / Sanaz Abbaspour-Aghdam / Kosar Malekpour / Mehdi Yousefi / Hossein Samadi Kafil / Farhad Jadidi-Niaragh / Leila Roshangar / Hamed Valizadeh / Morteza Izadi / Majid Ahmadi

Iranian Journal of Allergy, Asthma and Immunology, Vol 22, Iss

2023 Volume 3

Abstract: An imbalance between regulatory T (Treg) and T-helper (Th)-17 cells has been implicated in the pathogenesis of coronavirus disease 2019 (COVID-19). Mesenchymal stem cells (MSCs) exert immunomodulatory properties through secreting exosomes. This study ... ...

Abstract	An imbalance between regulatory T (Treg) and T-helper (Th)-17 cells has been implicated in the pathogenesis of coronavirus disease 2019 (COVID-19). Mesenchymal stem cells (MSCs) exert immunomodulatory properties through secreting exosomes. This study aimed to assess the effect of MSC-derived exosomes (MSC-Exo) on the differentiation of peripheral blood mononuclear cells (PBMCs) into Tregs from patients with COVID-19. Exosomes were isolated from adipose tissue–derived MSCs. PBMCs were separated from the whole blood of COVID-19 patients (n=20). Treg frequency was assessed before and 48 hours after treatment of PBMCs with MSC-Exo using flow cytometry. Expression of FOXP3 and cytokine genes, and the concentration of cytokines associated with Tregs, were assessed before and after treatment with MSC-Exo. The frequency of CD4+CD25+CD127- Tregs was significantly higher after treating PBMCs with MSC-Exo (6.695±2.528) compared to before treatment (4.981±2.068). The expressions of transforming growth factor (TGF)-β1, interleukin (IL)-10, and FOXP3 were significantly upregulated in MSC-Exo–treated PBMCs. The concentration of IL‐10 increased significantly after treatment (994.7±543.9 pg/mL) of PBMCs with MSC-Exo compared with before treatment (563.5±408.6 pg/mL). The concentration of TGF-β was significantly higher in the supernatant of PBMCs after treatment with MSC-Exo (477.0±391.1 pg/mL) than PBMCs before treatment (257.7±226.3 pg/mL). MSC-Exo has the potential to raise anti-inflammatory responses by induction of Tregs, potentiating its therapeutic effects in COVID-19.
Keywords	COVID-19 ; Exosomes ; Immunomodulation ; Mesenchymal stem cells ; Peripheral blood mononuclear cells ; Regulatory T cells ; Medicine ; R
Subject code	610
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Tehran University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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