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Article ; Online: A comparison of different DNA extraction methods and molecular techniques for the detection and identification of foodborne pathogens.

Papatheodorou, Spyridon Andreas / Halvatsiotis, Panagiotis / Houhoula, Dimitra

AIMS microbiology

2021 Volume 7, Issue 3, Page(s) 304–319

Abstract: Foodborne infections continue to plague Europe. Food safety monitoring is in crisis as the existing techniques for detecting pathogens do not keep up with the global rising of food production and consumption. Thus, the development of innovative ... ...

Abstract	Foodborne infections continue to plague Europe. Food safety monitoring is in crisis as the existing techniques for detecting pathogens do not keep up with the global rising of food production and consumption. Thus, the development of innovative techniques for detecting and identifying pathogenic bacteria has become critical. The aim of the present study was firstly to develop an innovative simple and low cost method of extracting bacterial DNA from contaminated food and water samples with
Language	English
Publishing date	2021-09-09
Publishing country	United States
Document type	Journal Article
ISSN	2471-1888
ISSN (online)	2471-1888
DOI	10.3934/microbiol.2021019
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Article ; Online: Enzymatic Modification of Pomace Olive Oil with Natural Antioxidants: Effect on Oxidative Stability.

Fotiadou, Renia / Lefas, Dimitrios / Vougiouklaki, Despina / Tsakni, Aliki / Houhoula, Dimitra / Stamatis, Haralambos

Biomolecules

2023 Volume 13, Issue 7

Abstract: Enzymatic lipophilization has been proposed as a cost-effective strategy to produce new liposoluble antioxidant compounds. In this study, modified oils rich in structured phenolipids were prepared via one-pot enzymatic acylation of hydroxytyrosol (HTYR), ...

Abstract	Enzymatic lipophilization has been proposed as a cost-effective strategy to produce new liposoluble antioxidant compounds. In this study, modified oils rich in structured phenolipids were prepared via one-pot enzymatic acylation of hydroxytyrosol (HTYR), vanillyl alcohol (VA) and homovanillyl alcohol (HVA) with pomace olive oil (POO) in solvent-free conditions using immobilized lipase on biogenic nanoparticles. The effect of temperature (30-70 °C) and enzyme concentration (0.1-1%,
MeSH term(s)	Antioxidants/pharmacology ; Antioxidants/chemistry ; Olive Oil ; Oxidation-Reduction ; Oils/chemistry ; Oxidative Stress
Chemical Substances	Antioxidants ; Olive Oil ; Oils
Language	English
Publishing date	2023-06-23
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2701262-1
ISSN	2218-273X ; 2218-273X
ISSN (online)	2218-273X
ISSN	2218-273X
DOI	10.3390/biom13071034
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Article ; Online: Lactoferrin in breast milk-based powders.

Tsakali, Efstathia / Aggarwal, Rakesh / Houhoula, Dimitra / Konteles, Spiridon / Batrinou, Athimia / Verheyen, Davy / Van Impe, Jan Fm / Chatzilazarou, Arhontoula

The Journal of dairy research

2024 Volume 90, Issue 4, Page(s) 409–412

Abstract: This study aimed to determine lactoferrin (LF) in breast milk-based powders and formulas. Lactoferrin is an important whey protein in all mammalian milks and is responsible in large part for the known antimicrobial effects of human milk in particular. As ...

Abstract	This study aimed to determine lactoferrin (LF) in breast milk-based powders and formulas. Lactoferrin is an important whey protein in all mammalian milks and is responsible in large part for the known antimicrobial effects of human milk in particular. As breast feeding is not always possible, formulas based on cows milk have been developed in order to meet the nutritional needs of the newborn, while more recently human breast milk-based powders have been introduced to offer the biological functionality of human milk to pre-term and critically ill babies. In the present work, the amount of LF in commercial breast milk-based powders was tested by a validated RF-HPLC method for the determination of LF in breast milk in order to examine both the applicability of the method but at a second level the amount of LF in these commercial products. The detection of LF was possible but the complexity of the matrix lead us to the use the standard addition methodology in order to achieve quantification. The results indicated that breast milk-based powders had higher amount of LF than cows milk-based formulas, both non-fortified and fortified.
MeSH term(s)	Infant ; Female ; Humans ; Cattle ; Animals ; Milk, Human ; Milk/metabolism ; Lactoferrin ; Powders/metabolism ; Breast Feeding ; Chromatography, High Pressure Liquid/veterinary ; Mammals/metabolism
Chemical Substances	Lactoferrin (EC 3.4.21.-) ; Powders
Language	English
Publishing date	2024-01-12
Publishing country	England
Document type	Journal Article
ZDB-ID	242089-2
ISSN	1469-7629 ; 0022-0299
ISSN (online)	1469-7629
ISSN	0022-0299
DOI	10.1017/S0022029923000778
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Article ; Online: A Bi-enzymatic Immobilized Nanobiocatalyst for the Biotransformation of Oleuropein to Hydroxytyrosol.

Giannakopoulou, Archontoula / Chatzikonstantinou, Alexandra V / Tsakni, Aliki / Chochos, Christos L / Houhoula, Dimitra / Stamatis, Haralambos

Methods in molecular biology (Clifton, N.J.)

2022 Volume 2487, Page(s) 279–296

Abstract: Multi-enzymatic assemblies offer the opportunity of bringing in proximity several enzymes that are enabled to work together for the catalysis of multi-step reactions. Especially, the development of robust nanobiocatalytic systems comprising of several ... ...

Abstract	Multi-enzymatic assemblies offer the opportunity of bringing in proximity several enzymes that are enabled to work together for the catalysis of multi-step reactions. Especially, the development of robust nanobiocatalytic systems comprising of several enzymes has gained considerable attention over the last few years for the catalysis of complex reactions and the production of high added-value products. In the present chapter, we describe the methodology for the development of a bi-enzymatic nanobiocatalyst consisting of the enzymes β-glucosidase from Thermotoga maritima and lipase A from Candida antarctica (CalA) co-immobilized on chitosan-coated magnetic nanoparticles. This nanobiocatalyst can be efficiently applied for the biotransformation of oleuropein to hydroxytyrosol, a reaction of increased biotechnological interest. Several techniques, as well as methodologies that are required for the characterization of the structure and the activity of such systems are also comprehensively described.
MeSH term(s)	Biotransformation ; Candida ; Enzymes, Immobilized/chemistry ; Iridoid Glucosides ; Phenylethyl Alcohol/analogs & derivatives
Chemical Substances	Enzymes, Immobilized ; Iridoid Glucosides ; 3,4-dihydroxyphenylethanol (10597-60-1) ; oleuropein (2O4553545L) ; Phenylethyl Alcohol (ML9LGA7468)
Language	English
Publishing date	2022-06-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2269-8_17
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Article: Probiotic Properties and Antioxidant Activity In Vitro of Lactic Acid Bacteria.

Vougiouklaki, Despina / Tsironi, Theofania / Tsantes, Andreas G / Tsakali, Efstathia / Van Impe, Jan F M / Houhoula, Dimitra

Microorganisms

2023 Volume 11, Issue 5

Abstract: The properties of probiotics such as lactic acid bacteria (LAB) have been widely studied over the last decades. In the present study, four different LAB species, ... ...

Abstract	The properties of probiotics such as lactic acid bacteria (LAB) have been widely studied over the last decades. In the present study, four different LAB species, namely
Language	English
Publishing date	2023-05-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms11051264
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Article ; Online: Neonatal hematological parameters: the translational aspect of developmental hematopoiesis.

Sokou, Rozeta / Parastatidou, Stavroula / Konstantinidi, Aikaterini / Ioakeimidis, Georgios / Tsantes, Andreas G / Houhoula, Dimitra / Iacovidou, Nicoletta / Tsantes, Argirios E

Annals of hematology

2023 Volume 102, Issue 4, Page(s) 707–714

Abstract: Hematopoiesis is a process constantly evolving from fetal life through adulthood. Neonates present with qualitative and quantitative differences in hematological parameters compared to older children and adults, reflecting developmental changes in ... ...

Abstract	Hematopoiesis is a process constantly evolving from fetal life through adulthood. Neonates present with qualitative and quantitative differences in hematological parameters compared to older children and adults, reflecting developmental changes in hematopoiesis correlated with gestational age. Such differences are more intense for preterm and small-for-gestational-age neonates or neonates with intrauterine growth restriction. This review article is aimed at describing the hematologic differences among neonatal subgroups and the major underlying pathogenic mechanisms. Issues that should be taken into account when interpreting neonatal hematological parameters are also highlighted.
MeSH term(s)	Infant, Newborn ; Adult ; Female ; Child ; Humans ; Adolescent ; Infant, Small for Gestational Age ; Fetal Growth Retardation ; Gestational Age ; Infant, Newborn, Diseases ; Hematopoiesis
Language	English
Publishing date	2023-02-27
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	1064950-5
ISSN	1432-0584 ; 0939-5555 ; 0945-8077
ISSN (online)	1432-0584
ISSN	0939-5555 ; 0945-8077
DOI	10.1007/s00277-023-05144-2
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Article: Identification of Polymorphisms of the CSN2 Gene Encoding β-Casein in Greek Local Breeds of Cattle

Antonopoulos, Dionysios / Vougiouklaki, Despina / Laliotis, George P. / Tsironi, Theofania / Valasi, Irene / Chatzilazarou, Archodoula / Halvatsiotis, Panagiotis / Houhoula, Dimitra

Veterinary sciences. 2021 Oct. 30, v. 8, no. 11

2021

Abstract: This e research focused on the detection and identification of genetic polymorphisms in exon 7 of the β-casein CSN2 gene in blood samples from Greek Holstein cows and from local breeds of cattle, such as Vrachykeratiki, Katerinis, and Sykias. For this ... ...

Abstract	This e research focused on the detection and identification of genetic polymorphisms in exon 7 of the β-casein CSN2 gene in blood samples from Greek Holstein cows and from local breeds of cattle, such as Vrachykeratiki, Katerinis, and Sykias. For this purpose, DNA was isolated from 780 blood samples obtained from Greek Holstein cows, 86 from three local breeds of cattle, namely Brachyceros, Katerinis, and Sykias, and 14 from Greek buffalo. The desired region of exon 7 was amplified by PCR, resulting in 121 and 251 bp products in bovine and buffalo samples. The PCR product was digested with restriction fragment length polymorphism (RFLP) on agarose gels. The restriction enzymes DdeI and TaqI were used. All of the blood samples had the amplified size. The results showed that 74.4% of the Greek Holstein cows had the A2A2 β-casein genotype, the three native breads Vrachykeratiki had 57.7%, and the other two had 100% of the A2A2 β-casein. From the 14 Greek buffalo, 100% had the A2A2 β-casein.
Keywords	DNA ; Holstein ; agarose ; blood ; buffaloes ; exons ; genotype ; restriction fragment length polymorphism
Language	English
Dates of publication	2021-1030
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2768971-2
ISSN	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci8110257
Database	NAL-Catalogue (AGRICOLA)

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Article: Bacterial community in response to packaging conditions in farmed gilthead seabream

Thomas, Apollon / Konteles, Spyros J. / Ouzounis, Sotiris / Papatheodorou, Spyros / Tsakni, Aliki / Houhoula, Dimitra / Tsironi, Theofania

Shanghai Ocean University. Aquaculture and fisheries. 2021 Sept. 17,

2021

Abstract: The objective of this study was to evaluate the effect of modified atmosphere packaging (MAP) on fish skin, gills and intestines bacterial microbiome.Whole gilthead seabream was packed aerobically or under modified atmospheres (60% CO₂, 30% N₂, 10% O₂) ... ...

Abstract	The objective of this study was to evaluate the effect of modified atmosphere packaging (MAP) on fish skin, gills and intestines bacterial microbiome.Whole gilthead seabream was packed aerobically or under modified atmospheres (60% CO₂, 30% N₂, 10% O₂) and stored isothermally at 0 °C. Next Generation Sequencing (NGS) analysis was applied for the characterization of fish microbiome on fish skin, gills, and intestines initially (time of packaging, 1 day after harvesting) and after 10 days of isothermal storage at 0 °C. NGS results indicated statistically significant differences in families’ richness and diversity in the tested fish tissues between aerobic and MAP packaging during storage at 0 °C. The most persistent bacteria were Proteobacteria for both packaging types. For fish skin microbiota, the initially prevailing families were Comamonadaceae, Enterobacteriaceae and Moraxellaceae while in the intestines Comamonadaceae, Anaplasmataceae, Bacillaceae and Enterobacteriaceae were the dominant bacteria. At the end of storage period, the fish microbiota was dominated by psychotropic and psychrophilic families (Pseudoalteromonadaceae, Psychromonadaceae, and Shewanellaceae), while families such as Comamonadaceae were persistent under MAP conditions. By 8 days of fish storage at 0 °C, ΜΑΡ samples exhibited higher sensory scorings than the respective aerobically stored fish, indicating better retention of fish freshness and fish quality attributes under MAP. Based on the results of the study, MAP modified significantly the microbiological status and extended the shelf life of fish. NGS was a powerful tool that provided a more complete assessment compared to a culture-based analysis.
Keywords	Anaplasmataceae ; Bacillaceae ; Comamonadaceae ; Enterobacteriaceae ; Moraxellaceae ; Sparus aurata ; aquaculture ; bacterial communities ; carbon dioxide ; fish ; fish skin ; food storage ; freshness ; microbiome ; shelf life ; storage time
Language	English
Dates of publication	2021-0917
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ISSN	2468-550X
DOI	10.1016/j.aaf.2021.09.002
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Article: Association of TNF-α 308G/A and LEPR Gln223Arg Polymorphisms with the Risk of Type 2 Diabetes Mellitus

Trapali, Maria / Houhoula, Dimitra / Batrinou, Anthimia / Kanellou, Anastasia / Strati, Irini F. / Siatelis, Argyris / Halvatsiotis, Panagiotis

Genes. 2021 Dec. 27, v. 13, no. 1

2021

Abstract: The objective of the present study was to identify the association of the TNF-α- 308G/A and leptin receptor (LEPR) Gln223Arg polymorphisms with the risk of development of type 2 diabetes mellitus (T2DM). Methods: A total of 160 volunteers were studied: ... ...

Abstract	The objective of the present study was to identify the association of the TNF-α- 308G/A and leptin receptor (LEPR) Gln223Arg polymorphisms with the risk of development of type 2 diabetes mellitus (T2DM). Methods: A total of 160 volunteers were studied: 108 with T2DM and 52 participants as control, who served as the control group. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) for the genomic region of TNF-α- 308G/A and LEPR Gln223Arg were carried out. Results: The frequency of LEPR Gln223Arg genotypes in T2DM and control groups showed significant differences in the distribution of genotypes (p < 0.05). The frequency also of TNF-α- 308G/A genotypes in T2DM and control subjects showed significant differences in the distribution of genotypes (p < 0.05). Conclusion: Our results indicate that there are significant differences in the distribution of genotypes and alleles between the individuals with T2DM and control subjects (p < 0.05).
Keywords	genomics ; leptin receptors ; noninsulin-dependent diabetes mellitus ; polymerase chain reaction ; risk
Language	English
Dates of publication	2021-1227
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2527218-4
ISSN	2073-4425
ISSN	2073-4425
DOI	10.3390/genes13010059
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Pathogen Reduction Technologies and Their Impact on Metabolic and Functional Properties of Treated Platelet Concentrates: A Systematic Review.

Tsalas, Stavros / Petrou, Eleni / Tsantes, Andreas G / Sokou, Rozeta / Loukopoulou, Electra / Houhoula, Dimitra / Mantzios, Petros G / Kriebardis, Anastasios G / Tsantes, Argirios E

Seminars in thrombosis and hemostasis

2022 Volume 49, Issue 5, Page(s) 523–541

Abstract: Pathogen reduction technologies (PRTs) such as Mirasol and Intercept were developed to eliminate transfusion-transmitted infections. The impact of PRTs on platelet function during the storage period, their effect on platelet storage lesions, and the ... ...

Abstract	Pathogen reduction technologies (PRTs) such as Mirasol and Intercept were developed to eliminate transfusion-transmitted infections. The impact of PRTs on platelet function during the storage period, their effect on platelet storage lesions, and the optimal storage duration following PRTs have not been clearly defined. The aim of this study was to systematically review the existing literature and investigate the impact of PRTs on functional alterations of PRT-treated platelets during the storage period. The authors identified 68 studies suitable to be included in this review. Despite the high heterogeneity in the literature, the results of the published studies indicate that PRTs may increase platelet metabolic activity, accelerate cell apoptosis, and enhance platelet activation, which can subsequently lead to a late exhaustion of activation potential and reduced aggregation response. However, these effects have a minor impact on platelet function during the early storage period and become more prominent beyond the fifth day of the storage period. Large in vivo trials are required to evaluate the effectiveness of PRT-treated platelets during the storage period and investigate whether their storage can be safely extended to more than 5 days, and up to the traditional 7-day storage period.
MeSH term(s)	Humans ; Blood Platelets/metabolism ; Platelet Activation ; Blood Preservation/methods ; Platelet Transfusion/methods
Language	English
Publishing date	2022-10-17
Publishing country	United States
Document type	Systematic Review ; Journal Article
ZDB-ID	196901-8
ISSN	1098-9064 ; 0094-6176
ISSN (online)	1098-9064
ISSN	0094-6176
DOI	10.1055/s-0042-1757897
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