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Article ; Online: Environmental impacts on intraspecific variation in Ambrosia artemisiifolia genome size in Slovakia, Central Europe.

Hrabovský, Michal / Kubalová, Silvia / Mičieta, Karol / Ščevková, Jana

Environmental science and pollution research international

2024

Abstract: The quantity of DNA in angiosperms exhibits variation attributed to many external influences, such as environmental factors, geographical features, or stress factors, which exert constant selection pressure on organisms. Since invasive species possess ... ...

Abstract	The quantity of DNA in angiosperms exhibits variation attributed to many external influences, such as environmental factors, geographical features, or stress factors, which exert constant selection pressure on organisms. Since invasive species possess adaptive capabilities to acclimate to novel environmental conditions, ragweed (Ambrosia artemisiifolia L.) was chosen as a subject for investigating their influence on genome size variation. Slovakia has diverse climatic conditions, suitable for testing the hypothesis that air temperature and precipitation, the main limiting factors of ragweed occurrence, would also have an impact on its genome size. Our results using flow cytometry confirmed this hypothesis and also found a significant association with geographical features such as latitude, altitude, and longitude. We can conclude that plants growing in colder environments farther from oceanic influences exhibit smaller DNA amounts, while optimal growth conditions result in a greater variability in genome size, reflecting the diminished effect of selection pressure.
Language	English
Publishing date	2024-05-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-024-33410-x
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Article ; Online: Surgical planning during a pandemic: Identifying patients at high risk of severe disease or death due to COVID-19 in a cohort of patients on a cataract surgery waiting list.

Stuart, Mark / Mooney, Ciaran / Hrabovsky, Monica / Silvestri, Giuliana / Stewart, Stephen

The Ulster medical journal

2022 Volume 91, Issue 1, Page(s) 19–25

Abstract: Background: The delivery of cataract surgery during the COVID-19 pandemic is challenging because of the risk of nosocomial SARS-CoV-2 infection when patients attend hospital for elective care. In order to ascertain the risk to patients awaiting cataract ...

Abstract	Background: The delivery of cataract surgery during the COVID-19 pandemic is challenging because of the risk of nosocomial SARS-CoV-2 infection when patients attend hospital for elective care. In order to ascertain the risk to patients awaiting cataract surgery, this study aimed to identify the presence of systemic comorbidities that are associated with a high risk of severe disease or death due to COVID-19. Methods: A prospective study of 315 patients (630 eyes) was conducted from 3 Results: The median age of patients awaiting cataract surgery was 76 years (range 22-97). Of the 315 patients, 72% were aged over 70 and 16% were aged over 85. A systemic comorbidity that would confer high risk status was identified in 21% of patients. This high risk status was attributable to severe respiratory disease, cancer, and immunosuppression therapies in the majority of cases. The high risk group were younger than those deemed non-high risk, but there were no significant differences with respect to gender, anticipated degree of surgical difficulty, VA, or whether the patient was undergoing first or second eye surgery. Of those patients awaiting first eye cataract surgery, the mean VA in the listed eye was 0.84 logMAR and 39% (70/179) had a VA <0.3 logMAR (6/12 Snellen acuity) in their fellow eye. 57% of patients were awaiting first eye surgery, and 32% of those patients would be at risk of symptomatic anisometropia postoperatively. Conclusion: One-fifth of patients awaiting cataract surgery were found to be at high risk of severe disease or death from COVID-19 and these patients may experience delays in their surgical care. Additional planning is required in order to minimise the morbidity associated with delayed cataract surgery.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; COVID-19 ; Cataract/epidemiology ; Cataract Extraction ; Humans ; Middle Aged ; Pandemics ; Prospective Studies ; SARS-CoV-2 ; Waiting Lists ; Young Adult
Language	English
Publishing date	2022-02-11
Publishing country	Northern Ireland
Document type	Journal Article
ZDB-ID	603342-8
ISSN	2046-4207 ; 0041-6193
ISSN (online)	2046-4207
ISSN	0041-6193
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Article ; Online: Intradiurnal variation of predominant airborne fungal spore biopollutants in the Central European urban environment.

Ščevková, Jana / Hrabovský, Michal / Kováč, Jozef / Rosa, Samuel

Environmental science and pollution research international

2019 Volume 26, Issue 33, Page(s) 34603–34612

Abstract: Fungal spores are an important cause of allergic respiratory diseases worldwide. However, little is known about the intradiurnal pattern of spore concentrations of different fungal spore types in the air of the urban area. In this study, we evaluated ... ...

Abstract	Fungal spores are an important cause of allergic respiratory diseases worldwide. However, little is known about the intradiurnal pattern of spore concentrations of different fungal spore types in the air of the urban area. In this study, we evaluated bihourly variation in spore concentration of eight predominant fungal spore types in the atmosphere of Bratislava city (Agrocybe, Alternaria, Cladosporium, Coprinus, Exosporium, Epicoccum, Ganoderma, Leptosphaeria) with the aim to understand the relationships between the spore concentrations against associated environmental variables. Spore samples were collected using a Hirst-type volumetric aerospore trap from January to December 2016. Alternaria, Cladosporium, Epicoccum and Exosporium peaked during the daytime period between 10:00 and 16:00, while for Agrocybe, Ganoderma, Coprinus and Leptosphaeria, the nighttime peaks (20:00 and 04:00) were observed. Effect of a complex of environmental variables on bihourly concentrations of selected airborne fungal spore taxa was evaluated through multiple regression analysis. Air temperature, wind speed, sunshine duration and precipitation were positively associated with daytime spore types, while the association with nighttime spores was negative. In contrast, relative air humidity influenced negatively Exosporium daytime spore type but positively the Leptosphaeria nighttime spore type. Moreover, a circadian cycle of light and darkness was considered as an important predictor of nighttime spore levels. Among the atmospheric pollutants, PM
MeSH term(s)	Air Microbiology ; Air Pollutants/analysis ; Alternaria ; Ascomycota ; Cities ; Cladosporium ; Environmental Monitoring ; Multivariate Analysis ; Regression Analysis ; Seasons ; Spores, Fungal ; Temperature ; Wind
Chemical Substances	Air Pollutants
Language	English
Publishing date	2019-10-25
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-019-06616-7
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Article ; Online: Production of high quality syngas from argon/water plasma gasification of biomass and waste.

Hlina, M / Hrabovsky, M / Kavka, T / Konrad, M

Waste management (New York, N.Y.)

2014 Volume 34, Issue 1, Page(s) 63–66

Abstract: Extremely hot thermal plasma was used for the gasification of biomass (spruce sawdust, wood pellets) and waste (waste plastics, pyrolysis oil). The plasma was produced by a plasma torch with DC electric arc using unique hybrid stabilization. The torch ... ...

Abstract	Extremely hot thermal plasma was used for the gasification of biomass (spruce sawdust, wood pellets) and waste (waste plastics, pyrolysis oil). The plasma was produced by a plasma torch with DC electric arc using unique hybrid stabilization. The torch input power of 100-110 kW and the mass flow rate of the gasified materials of tens kg/h was set up during experiments. Produced synthetic gas featured very high content of hydrogen and carbon monoxide (together approximately 90%) that is in a good agreement with theory. High quality of the produced gas is given by extreme parameters of used plasma--composition, very high temperature and low mass flow rate.
MeSH term(s)	Biofuels ; Biomass ; Carbon Monoxide/analysis ; Carbon Monoxide/chemistry ; Equipment Design ; Gases ; Hydrogen/analysis ; Hydrogen/chemistry ; Plastics ; Refuse Disposal/instrumentation ; Refuse Disposal/methods ; Solid Waste ; Wood
Chemical Substances	Biofuels ; Gases ; Plastics ; Solid Waste ; Carbon Monoxide (7U1EE4V452) ; Hydrogen (7YNJ3PO35Z)
Language	English
Publishing date	2014-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2001471-5
ISSN	1879-2456 ; 0956-053X
ISSN (online)	1879-2456
ISSN	0956-053X
DOI	10.1016/j.wasman.2013.09.018
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Article: Non-invasive monitoring of hydraulic surge propagation in a wounded tobacco plant.

Nožková, Vladimíra / Šmíd, Petr / Horváth, Pavel / Hrabovský, Miroslav / Ilík, Petr

Plant methods

2018 Volume 14, Page(s) 38

Abstract: Background: When a plant is wounded, a rapid hydraulic surge, acting probably as a systemic signal, spreads from the site of injury throughout the plant and leads to small transient deformation of tissues. So far, the propagation of hydraulic surge has ... ...

Abstract	Background: When a plant is wounded, a rapid hydraulic surge, acting probably as a systemic signal, spreads from the site of injury throughout the plant and leads to small transient deformation of tissues. So far, the propagation of hydraulic surge has been monitored by contact and thus potentially invasive methods. Results: Here we present a non-invasive optical method, which allows simultaneous monitoring of micrometric shift of two opposite stem margins. The usefulness of this method was demonstrated by the measurement of the hydraulic surge propagation in a tobacco ( Conclusions: This optical method represents a novel tool to investigate the mechanism of systemic response of plants to local damage. Our results points out the complexity of the relationship between hydraulic surge propagation and stem deformation.
Language	English
Publishing date	2018-05-25
Publishing country	England
Document type	Journal Article
ZDB-ID	2203723-8
ISSN	1746-4811
ISSN	1746-4811
DOI	10.1186/s13007-018-0307-6
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Article ; Online: Methods for Determination of Mean Speckle Size in Simulated Speckle Pattern

Hamarová I. / Šmíd P. / Horváth P. / Hrabovský M.

Measurement Science Review, Vol 14, Iss 3, Pp 177-

2014 Volume 182

Abstract: This paper deals with computation of mean speckle size in a speckle pattern generated through a numerical simulation of speckle after reflection of a Gaussian beam off a rough object’s surface. Within this simulation various speckle patterns are obtained ...

Abstract	This paper deals with computation of mean speckle size in a speckle pattern generated through a numerical simulation of speckle after reflection of a Gaussian beam off a rough object’s surface. Within this simulation various speckle patterns are obtained by means of change in a parameter of the Gaussian beam. The mean speckle size is computed through two approaches using both the two-dimensional and the one-dimensional normalized autocorrelation function in intensity. Additionally, we propose a distinct optimization of the determination of the mean speckle size by reduction of intensity values representing detected speckle patterns. Results of the determination of the mean speckle size are compared with theoretical predictions
Keywords	Simulation ; speckle ; speckle pattern ; mean speckle size ; autocorrelation function ; Mathematics ; QA1-939 ; Science ; Q
Language	English
Publishing date	2014-06-01T00:00:00Z
Publisher	Versita
Document type	Article ; Online
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Article ; Online: Geometric Control of Cell Behavior by Biomolecule Nanodistribution.

Pospíšil, Jakub / Hrabovský, Miloš / Bohačiaková, Dáša / Hovádková, Zuzana / Jurásek, Miroslav / Mlčoušková, Jarmila / Paruch, Kamil / Nevolová, Šárka / Damborsky, Jiri / Hampl, Aleš / Jaros, Josef

ACS biomaterials science & engineering

2022 Volume 8, Issue 11, Page(s) 4789–4806

Abstract: Many dynamic interactions within the cell microenvironment modulate cell behavior and cell fate. However, the pathways and mechanisms behind cell-cell or cell-extracellular matrix interactions remain understudied, as they occur at a nanoscale level. ... ...

Abstract	Many dynamic interactions within the cell microenvironment modulate cell behavior and cell fate. However, the pathways and mechanisms behind cell-cell or cell-extracellular matrix interactions remain understudied, as they occur at a nanoscale level. Recent progress in nanotechnology allows for mimicking of the microenvironment at nanoscale in vitro; electron-beam lithography (EBL) is currently the most promising technique. Although this nanopatterning technique can generate nanostructures of good quality and resolution, it has resulted, thus far, in the production of only simple shapes (e.g., rectangles) over a relatively small area (100 × 100 μm), leaving its potential in biological applications unfulfilled. Here, we used EBL for cell-interaction studies by coating cell-culture-relevant material with electron-conductive indium tin oxide, which formed nanopatterns of complex nanohexagonal structures over a large area (500 × 500 μm). We confirmed the potential of EBL for use in cell-interaction studies by analyzing specific cell responses toward differentially distributed nanohexagons spaced at 1000, 500, and 250 nm. We found that our optimized technique of EBL with HaloTags enabled the investigation of broad changes to a cell-culture-relevant surface and can provide an understanding of cellular signaling mechanisms at a single-molecule level.
MeSH term(s)	Nanotechnology/methods ; Nanostructures/chemistry ; Extracellular Matrix ; Cell Culture Techniques ; Cell Differentiation
Language	English
Publishing date	2022-10-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2373-9878
ISSN (online)	2373-9878
DOI	10.1021/acsbiomaterials.2c00650
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Article ; Online: Expansion and aerobiology of Ambrosia artemisiifolia L. in Slovakia.

Hrabovský, Michal / Ščevková, Jana / Mičieta, Karol / Lafférsová, Janka / Dušička, Jozef

Annals of agricultural and environmental medicine : AAEM

2016 Volume 23, Issue 1, Page(s) 64–70

Abstract: Introduction and objective: The invasive alien species Ambrosia artemisiifolia cause environmental, agronomical and medical problems in many regions of the world, including Slovakia. The purpose of this study was to survey the spread and distribution of ...

Abstract	Introduction and objective: The invasive alien species Ambrosia artemisiifolia cause environmental, agronomical and medical problems in many regions of the world, including Slovakia. The purpose of this study was to survey the spread and distribution of this species in Slovakia and to analyse its airborne pollen pattern. Materials and methods: To evaluate the spatiotemporal dynamics of Ambrosia invasion in the territory of Slovakia, herbarium specimens, published databases and field investigations were considered. Aerobiological sampling was based on the analysis of pollen records at five aerobiological stations in Slovakia. For Bratislava and Banská Bystrica Monitoring stations, trends in Ambrosia pollen seasons were determined using Mann-Kendall test and Sen's slope estimator. Results: Since the first record of A. artemisiifolia in Slovakia, the number of its colonies and its spread rate has increased considerably, and the colonisation of this species has been successful mainly in the south-western part of the country. Highest airborne pollen counts were recorded in Nitra, Trnava and Bratislava Monitoring Stations situated in the areas most infested by A. artemisiifolia in Slovakia. However, high pollen counts were also noted in Banská Bystrica and Košice Monitoring Stations situated in areas where the source species was less abundant. During the study period, the number of days on which the pollen concentration exceeded the threshold of sensitivity increased significantly (+1.33 days/year) in Banská Bystrica, whereas the peak value decreased significantly (-13.37 pollen/year) in Bratislava. Conclusion: The number of the populations of A. artemisiifolia has increased considerably in recent years. Besides the most infested areas, high airborne pollen counts were also recorded in territories where the plant species was less abundant. During the study period, the intensity of Ambrosia pollen seasons decreased in Bratislava, probably due to changes in land-use practices, while the increasing trend in the pollen seasons intensity in Banská Bystrica mainly reflects the situation in the ragweed-infested remote areas due to long-range pollen transport.
MeSH term(s)	Allergens/analysis ; Ambrosia/physiology ; Antigens, Plant/analysis ; Environmental Monitoring ; Introduced Species ; Plant Dispersal ; Plant Extracts/analysis ; Seasons ; Slovakia
Chemical Substances	Allergens ; Antigens, Plant ; Plant Extracts ; ragweed pollen
Language	English
Publishing date	2016
Publishing country	Poland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1215115-4
ISSN	1898-2263 ; 1232-1966
ISSN (online)	1898-2263
ISSN	1232-1966
DOI	10.5604/12321966.1196854
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Article ; Online: A simulation analysis of an extension of one-dimensional speckle correlation method for detection of general in-plane translation.

Hamarová, Ivana / Smíd, Petr / Horváth, Pavel / Hrabovský, Miroslav

TheScientificWorldJournal

2014 Volume 2014, Page(s) 704368

Abstract: The purpose of the study is to show a proposal of an extension of a one-dimensional speckle correlation method, which is primarily intended for determination of one-dimensional object's translation, for detection of general in-plane object's translation. ...

Abstract	The purpose of the study is to show a proposal of an extension of a one-dimensional speckle correlation method, which is primarily intended for determination of one-dimensional object's translation, for detection of general in-plane object's translation. In that view, a numerical simulation of a displacement of the speckle field as a consequence of general in-plane object's translation is presented. The translation components a x and a y representing the projections of a vector a of the object's displacement onto both x- and y-axes in the object plane (x, y) are evaluated separately by means of the extended one-dimensional speckle correlation method. Moreover, one can perform a distinct optimization of the method by reduction of intensity values representing detected speckle patterns. The theoretical relations between the translation components a x and a y of the object and the displacement of the speckle pattern for selected geometrical arrangement are mentioned and used for the testifying of the proposed method's rightness.
MeSH term(s)	Models, Theoretical ; Motion
Language	English
Publishing date	2014-01-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2075968-X
ISSN	1537-744X ; 1537-744X
ISSN (online)	1537-744X
ISSN	1537-744X
DOI	10.1155/2014/704368
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Article ; Online: Upgraded Cherenkov time-of-flight detector for the AFP project.

Optics express

2023 Volume 31, Issue 3, Page(s) 3998–4014

Abstract: We present the results of our performance studies of the upgraded Cherenkov time-of-flight (ToF) detector for the AFP (ATLAS Forward Proton) project. The latest version consists of solid L-shaped fused silica bars, new customized ALD-coated micro-channel ...

Abstract	We present the results of our performance studies of the upgraded Cherenkov time-of-flight (ToF) detector for the AFP (ATLAS Forward Proton) project. The latest version consists of solid L-shaped fused silica bars, new customized ALD-coated micro-channel plate photomultipliers (MCP-PMTs) miniPlanacon XPM85112-S-R2D2 with an extended lifetime which operate at low gains (order of 10
Language	English
Publishing date	2023-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.480624
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