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Article ; Online: Coenzyme Q0, a quinone derivative from Antrodia camphorata, inhibits epithelial-mesenchymal transition by activating the Nrf2 signaling pathway in TGF-β-stimulated adult retinal pigment epithelial cells to improve age-related macular degeneration and proliferative vitreoretinopathy

Hsin-Ling Yang / Hung-Rong Yen / I-Chen Chang / Chithravel Vadivalagan / Siang-Jyun Chen / Chuan-Chen Lee / Wei-Chen Jane / Jhih-Hsuan Hseu / You-Cheng Hseu

Journal of Functional Foods, Vol 112, Iss , Pp 105978- (2024)

2024

Abstract: We investigated the anti-EMT and antifibrotic effects of coenzyme Q0 (CoQ0), a quinone derivative from Antrodia camphorata, in TGF-β-stimulated adult retinal pigment epithelial 19 (ARPE-19) cells. Results showed that CoQ0 treatment reversed TGF-β- ... ...

Abstract	We investigated the anti-EMT and antifibrotic effects of coenzyme Q0 (CoQ0), a quinone derivative from Antrodia camphorata, in TGF-β-stimulated adult retinal pigment epithelial 19 (ARPE-19) cells. Results showed that CoQ0 treatment reversed TGF-β-stimulated morphological changes from an epithelial to a fibroblastic phenotype in ARPE-19 cells. CoQ0 exhibited anti-EMT effects by impeding TGF-β-stimulated migration and invasion in ARPE-19 cells. Notably, CoQ0 triggered the epithelial marker E-cadherin and suppressed the mesenchymal marker N-cadherin expression in nonstimulated or TGF-β-stimulated ARPE-19 cells. Moreover, CoQ0 attenuated EMT and fibrotic Vimentin, MMP-9/-2, Slug, α-SMA, and VEGF expression in nonstimulated or TGF-β-stimulated ARPE-19 cells. Interestingly, CoQ0 inhibited TGF-β-induced intracellular ROS production by activating Nrf2 nuclear translocation and upregulating the HO-1, γ-GCLC, and NQO-1 enzymes in ARPE-19 cells. Moreover, Nrf2 silencing reversed TGF-β-induced ROS-mediated anti-EMT (E-cadherin/N-cadherin/Slug) and antifibrotic (α-SMA) effects in CoQ0-treated ARPE-19 cells. Therefore, CoQ0 could be utilized to treat age-related macular degeneration and proliferative vitreoretinopathy.
Keywords	Coenzyme Q0 ; ARPE-19 ; TGF-β ; ROS ; EMT ; Nutrition. Foods and food supply ; TX341-641
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway

Jia-Hau Yen / Wan-Ting Chio / Chia-Ju Chuang / Hsin-Ling Yang / Sheng-Teng Huang

Molecules, Vol 27, Iss 1695, p

2022 Volume 1695

Abstract: This study aims to investigate the wound-healing effectiveness of the phenolic compound, naringin, both in vitro and in vivo. Male mice were shaved on their dorsal skin under isoflurane, a biopsy punch was made in four symmetrical circular resection ... ...

Abstract	This study aims to investigate the wound-healing effectiveness of the phenolic compound, naringin, both in vitro and in vivo. Male mice were shaved on their dorsal skin under isoflurane, a biopsy punch was made in four symmetrical circular resection windows (6 mm) to induce a wound. These excision wounds were used to study the topical effects of naringin in terms of various biochemical, molecular, and histological parameters. We observed a significant recovery in the wound area. Increased levels of MMP-2, 9, 14, TIMP-2, VEGF-A, and VEGF-R1 were induced by naringin in the HaCaT cells. The time course experiments further revealed that levels of VEGF-A and B increased within 36 h; whereas levels of VEGF-C decreased. In line with this, VEGF-R3 levels, but not VEGF-R1 and 2 levels, increased soon after stimulation; although the increase subsided after 36 h. Additionally, naringin cream upregulated wound healing in vitro. The blockage of VEGF by Bevacizumab abolished the function of naringin cream on cell migration. Histological alterations in the wounded skin were restored by naringin cream, which accelerated wound healing via upregulated expression of growth factors (VEGF-A, B, and C and VEGF-R3), and thus increased MMP-2, 9, 14 expressions.
Keywords	naringin ; wound healing ; MMPs ; VEGFs ; VEGFRs ; Organic chemistry ; QD241-441
Subject code	616
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways

You-Cheng Hseu, PhD / Yugandhar Vudhya Gowrisankar, PhD / Li-Wei Wang, MS / Yan-Zhen Zhang, MS / Xuan-Zao Chen, MS / Pei-Jane Huang, PhD / Hung-Rong Yen, PhD / Hsin-Ling Yang, PhD

Redox Biology, Vol 44, Iss , Pp 102007- (2021)

2021

Abstract: Pterostilbene (Pt) is a natural polyphenol found in blueberries and several grape varieties. Pt's pharmacological importance was well documented. Nevertheless, the depigmenting effects are not demonstrated. We evaluated the Pt's depigmenting effects ... ...

Abstract	Pterostilbene (Pt) is a natural polyphenol found in blueberries and several grape varieties. Pt's pharmacological importance was well documented. Nevertheless, the depigmenting effects are not demonstrated. We evaluated the Pt's depigmenting effects through autophagy induction in B16F10 cells and inhibition of UVA (3 J/cm2)-irradiated α-MSH in keratinocyte HaCaT cells via Nrf2-mediated antioxidant pathways. Pt (2.5–5μM) attenuated ROS production and downregulated the POMC/α-MSH pathway in HaCaT cells. The conditioned medium-derived from UVA-irradiated HaCaT pretreated with Pt suppressed melanogenesis in B16F10 through MITF-CREB-tyrosinase pathway downregulation. Interestingly, Pt-induced HaCaT autophagy was revealed by enhanced LC3-II accumulation, p62/SQSTM1 activation, and AVO formation. Pt significantly decreased melanosome gp100 but increased LC3-II levels in HaCaT cells exposed to B16F10-derived melanin. Pt activated and facilitated the Nrf2 antioxidant pathway in HaCaT cells leading to increased HO-1, γ-GCLC, and NQO-1 antioxidant protein expression. ERK, AMPK, and ROS pathways mediate the Nrf2 activation. However, Nrf2 knockdown suppressed Pt's antioxidant ability leading to uncontrolled ROS and α-MSH levels after UVA-irradiation suggested the essentiality of the Nrf2 pathway. Moreover, in α-MSH-stimulated B16F10 cells, Pt (10–30 μM) downregulated the MC1R, MITF, tyrosinase, TRP-1/-2, and melanin expression. Further, Pt showed potent anti-melanogenic effects through autophagy induction mechanism in B16F10 cells, verified by increased LC3-II/p62 levels, AVO formation, and Beclin-1/Bcl-2 ratio, decreased ATG4B levels and PI3K/AKT/mTOR pathway. Transmission electron microscopy provided direct evidence by showing autophagosomes engulfing melanosomes following Pt treatment in α-MSH-stimulated B16F10 cells. Moreover, Pt-induced anti-melanogenic activity through the downregulation of CREB-MITF pathway-mediated TRP-1/-2, tyrosinase expressions, melanosome formation, and melanin synthesis was substantially ...
Keywords	Pterostilbene ; ROS ; Anti-melanogenesis ; Nrf2 ; Autophagy ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Ganoderma tsugae induced ROS-independent apoptosis and cytoprotective autophagy in human chronic myeloid leukemia cells

Hseu, You-Cheng / Yi-Chun Shen / Ming-Ching Kao / Dony Chacko Mathew / Palaniyandi Karuppaiya / Mei-Ling Li / Hsin-Ling Yang

Food and chemical toxicology. 2019 Feb., v. 124

2019

Abstract: The medicinal fungus Ganoderma, known in Chinese as Lingzhi or Reishi, traditionally has various medicinal uses and has been employed in cancer treatment in Asia for centuries. This study used ethanol-extracted Ganoderma tsugae (GT) and examined its ... ...

Abstract	The medicinal fungus Ganoderma, known in Chinese as Lingzhi or Reishi, traditionally has various medicinal uses and has been employed in cancer treatment in Asia for centuries. This study used ethanol-extracted Ganoderma tsugae (GT) and examined its antitumor activities on human chronic myeloid leukemia cells as well as its molecular mechanism of action. Treatment with GT (200–400 μg/mL) significantly reduced cell viability and caused G2/M arrest in K562 cells. In addition, GT induced mitochondrial and death receptor mediated apoptosis, correlated with DNA fragmentation, followed by cytochrome c release, caspase-3/8/9 activation, PARP cleavage, Fas activation, Bid cleavage, and Bax/Bcl-2 dysregulation. Cytoprotective autophagy was found to be induced by GT, as was revealed by increased LC3-II accumulation, Beclin-1/Bcl-2 dysregulation, acidic vesicular organelle formation, and p62/SQSTM1 activation. Notably, pretreatment of cells with the autophagy inhibitors 3-MA and CQ enhanced GT-induced apoptosis. Interestingly, reactive oxygen species production in cells was not triggered by GT administration; equally, the antioxidant N-acetylcysteine was found to be incapable of preventing apoptosis and autophagy induced by GT treatment. Finally, this study discovered that cytoprotective autophagy induced by GT was associated with EGFR and PI3K/AKT/mTOR signaling cascade suppression. In summary, GT demonstrated antitumor activity against human chronic myeloid leukemia.
Keywords	DNA fragmentation ; Ganoderma tsugae ; acetylcysteine ; antineoplastic activity ; apoptosis ; autophagy ; cell viability ; cytochrome c ; death domain receptors ; humans ; mechanism of action ; medicinal fungi ; mitochondria ; myeloid leukemia ; neoplasm cells ; reactive oxygen species ; Asia
Language	English
Dates of publication	2019-02
Size	p. 30-44.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2018.11.043
Database	NAL-Catalogue (AGRICOLA)

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Article: Antrodia camphorata inhibits metastasis and epithelial-to-mesenchymal transition via the modulation of claudin-1 and Wnt/β-catenin signaling pathways in human colon cancer cells

Hseu, You-Cheng / Hsin-Ling Yang / Hui-Yi Lin / Kai-Yuan Lin / Tzong-Der Way / Varadharajan Thiyagarajan / Yu-Hsien Chao

Journal of ethnopharmacology. 2017 Aug. 17, v. 208

2017

Abstract: Antrodia camphorata (AC) is a well known traditional Chinese medicinal mushroom in Taiwan, has been used to treat various diseases including cancer.In this study, we investigated the anti-metastatic and anti-EMT properties of a fermented culture broth of ...

Abstract	Antrodia camphorata (AC) is a well known traditional Chinese medicinal mushroom in Taiwan, has been used to treat various diseases including cancer.In this study, we investigated the anti-metastatic and anti-EMT properties of a fermented culture broth of AC in human colon SW480claudin−1− and metastatic SW620claudin−1+ cancer cells in vitro.AC down-regulates claudin-1 and inhibits the proliferation and colony-formation abilities of both SW620claudin−1+ and SW480claudin−1− cells. In highly metastatic SW620claudin−1+ cells, non-cytotoxic concentrations of AC significantly inhibited migration/invasion, accompanied by the down-regulation of MMP-2 and MMP-9 proteins. AC decreased nuclear translocation of Wnt/β-catenin through a GSK3β-dependent pathway. AC consistently inhibited EMT by up-regulating the epithelial and downregulating the mesenchymal marker proteins. In SW480claudin−1− cells, AC suppressed migration/invasion potentially through the inhibition of the PI3K/AKT/NFκB signaling pathways without altering the expression levels of β-catenin and GSK3β proteins.Altogether, this study demonstrates the anti-metastatic and anti-EMT activities of AC, which may contribute to the development of a chemopreventive agent for colon cancer.
Keywords	chemoprevention ; colon ; colorectal neoplasms ; culture media ; epithelium ; humans ; medicinal fungi ; metastasis ; neoplasm cells ; signal transduction ; Taiwanofungus camphoratus ; tau-protein kinase ; traditional medicine ; transcription factor NF-kappa B ; Taiwan
Language	English
Dates of publication	2017-0817
Size	p. 72-83.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2017.07.001
Database	NAL-Catalogue (AGRICOLA)

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Article: Antrodia salmonea induces G2 cell-cycle arrest in human triple-negative breast cancer (MDA-MB-231) cells and suppresses tumor growth in athymic nude mice

Chang, Chia-Ting / Hsin-Ling Yang / Hui-Chi Huang / Jer-Yuh Liu / Jiunn-Wang Liao / Li-Sung Hsu / Pei-Jane Huang / Varadharajan Thiyagarajan / You-Cheng Hseu

Journal of ethnopharmacology. 2017 Jan. 20, v. 196

2017

Abstract: Antrodia salmonea (AS), is a well-known folk medicinal mushroom in Taiwan, has been reported to exhibit anti-oxidant, anti-angiogenic, and anti-inflammatory effects.In the present study, we examined the effects of AS on cell-cycle arrest in vitro in MDA- ... ...

Abstract	Antrodia salmonea (AS), is a well-known folk medicinal mushroom in Taiwan, has been reported to exhibit anti-oxidant, anti-angiogenic, and anti-inflammatory effects.In the present study, we examined the effects of AS on cell-cycle arrest in vitro in MDA-MB-231 cells and on tumor regression in vivo using an athymic nude mice model.AS (0–200μg/mL) treatment significantly induced G2 cell-cycle arrest in MDA-MB-231 cells by reducing the levels of cyclin B1, cyclin A, cyclin E, and CDC2 proteins. In addition, N-acetylcysteine (NAC) pretreatment prevented AS induced G2 cell-cycle arrest, indicating that ROS accumulation and subsequent cell cycle arrest might be a major mechanism of AS-induced cytotoxicity. Further, AS treatment decreased COX-2 expression and induced PARP cleavage was significantly reversed by NAC pretreatment in MDA-MB-231 cells. The in vivo study results revealed that AS treatment was effective in terms of delaying the tumor incidence and reducing the tumor growth in MDA-MB-231-xenografted nude mice. TUNEL assay, immunohistochemical staining and Western blotting confirmed that AS significantly modulated the xenografted tumor progression as demonstrated by induction of apoptosis, autophagy, and cell-cycle arrest.Our data strongly suggest that Antrodia salmonea could be an anti-cancer agent for human breast cancer.
Keywords	acetylcysteine ; antineoplastic agents ; antioxidants ; Antrodia ; apoptosis ; autophagy ; breast neoplasms ; cell cycle checkpoints ; cyclins ; cytotoxicity ; humans ; immunohistochemistry ; in vivo studies ; medicinal fungi ; mice ; neoplasm cells ; remission ; staining ; traditional medicine ; Western blotting ; Taiwan
Language	English
Dates of publication	2017-0120
Size	p. 9-19.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2016.12.018
Database	NAL-Catalogue (AGRICOLA)

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Article: Inhibition of ROS production, autophagy or apoptosis signaling reversed the anticancer properties of Antrodia salmonea in triple-negative breast cancer (MDA-MB-231) cells

Chang, Chia-Ting / Hsin-Ling Yang / Hui-Chi Huang / Jer-Yuh Liu / Kai-Yuan Lin / Mallikarjuna Korivi / Pei-Jane Huang / Varadharajan Thiyagarajan / You-Cheng Hseu

Food and chemical toxicology. 2017 May, v. 103

2017

Abstract: We investigated the inÂ vitro and inÂ vivo anticancer properties of Antrodia salmonea (AS), a well-known edible/medicinal mushroom in Taiwan, on human triple-negative breast cancer (MDA-MB-231) cells and xenografted nude mice; and revealed the underlying ...

Abstract	We investigated the inÂ vitro and inÂ vivo anticancer properties of Antrodia salmonea (AS), a well-known edible/medicinal mushroom in Taiwan, on human triple-negative breast cancer (MDA-MB-231) cells and xenografted nude mice; and revealed the underlying molecular mechanisms involved in autophagic- and apoptotic-cell death. Treatment of MDA-MB-231Â cells with fermented culture broth of AS (0â200Â Î¼g/mL) inhibited cell viability/growth. AS-induced autophagy was evidenced via increased LC3-II accumulation, GFP-LC3 puncta and AVOs formation in MDA-MB-231Â cells. These events are associated with increased ATG7, decreased p-mTOR, vanished SQSTM1/p62 expressions and dysregulated Beclin-1/Bcl-2 ratio. AS-induced apoptosis/necrosis through increased DNA fragmentation, Annexin-V/PI stained cells and Bax expression. Both mitochondrial (caspase-9/caspase-3/PARP) and death-receptor (caspase-8/FasL/Fas) signaling pathways are involved in execution of apoptosis. Interestingly, blockade of AS-induced ROS production by N-acetylcysteine pretreatment substantially attenuated AS-induced autophagy, mitochondrial dysfunction and autophagic/apoptotic-cell death. Inhibition of apoptosis by Z-VAD-FMK suppressed AS-induced autophagic-death (decreased LC3-II/AVOs). Similarly, inhibition of autophagy by 3-methyladenine/chloroquine diminished AS-induced apoptosis (decreased DNA fragmentation/caspase-3) in MDA-MB-231Â cells. Bioluminescence imaging further confirmed that AS inhibited breast tumor growth in living MDA-MB-231-luciferase-injected nude mice. Taken together, AS crucially involved in execution/propagation of autophagic- or apoptotic-death of MDA-MB-231Â cells, and decreased tumor growth in xenografted nude mice.
Keywords	acetylcysteine ; antineoplastic activity ; Antrodia ; apoptosis ; autophagy ; bioluminescence ; breast neoplasms ; cell viability ; culture media ; death ; DNA ; DNA fragmentation ; humans ; image analysis ; medicinal fungi ; mice ; mitochondria ; necrosis ; signal transduction ; toxicology ; Taiwan
Language	English
Dates of publication	2017-05
Size	p. 1-17.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2017.02.019
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Antitumor properties of Coenzyme Q0 against human ovarian carcinoma cells via induction of ROS-mediated apoptosis and cytoprotective autophagy

You-Cheng Hseu / Tai-Jung Tsai / Mallikarjuna Korivi / Jer-Yuh Liu / Hui-Jye Chen / Chung-Ming Lin / Yi-Chun Shen / Hsin-Ling Yang

Scientific Reports, Vol 7, Iss 1, Pp 1-

2017 Volume 21

Abstract: Abstract Coenzyme Q0 (CoQ0, 2,3-dimethoxy-5-methyl-1,4-benzoquinone) has been reported to exert anticancer properties against human breast/lung cancer cells. This study investigated the in vitro and in vivo anticancer properties of CoQ0 on human ovarian ... ...

Abstract	Abstract Coenzyme Q0 (CoQ0, 2,3-dimethoxy-5-methyl-1,4-benzoquinone) has been reported to exert anticancer properties against human breast/lung cancer cells. This study investigated the in vitro and in vivo anticancer properties of CoQ0 on human ovarian carcinoma (SKOV-3) cells and xenografted nude mice, and revealed the underlying molecular mechanism. CoQ0 induced G2/M arrest through downregulation of cyclin B1/A and CDK1/K2 expressions. CoQ0-induced autophagy as a survival mechanism was evidenced by increased accumulation of LC3-II, GFP-LC3 puncta, AVOs formation and Beclin-1/Bcl-2 dysregulation. Increased TUNEL-positive cells and Annexin-V/PI stained cells indicated CoQ0-induced late apoptosis. Both mitochondrial (caspase-3, PARP and Bax/Bcl-2 dysregulation) and ER stress (caspase-12 and Hsp70) signals are involved in execution of apoptosis. Interestingly, CoQ0-induced apoptosis/autophagy is associated with suppression of HER-2/neu and PI3K/AKT signalling cascades. CoQ0 triggered intracellular ROS production, whereas antioxidant N-acetylcysteine prevented CoQ0-induced apoptosis, but not autophagy. Inhibition of apoptosis by Z-VAD-FMK suppressed CoQ0-induced autophagy (diminished LC3-II/AVOs), indicates CoQ0-induced apoptosis led to evoke autophagy. Contrary, inhibition of autophagy by 3-MA/CQ potentiated CoQ0-induced apoptosis (increased DNA fragmentation/PARP cleavage). Furthermore, CoQ0 treatment to SKOV-3 xenografted nude mice reduced tumor incidence and burden. Histopathological analyses confirmed that CoQ0 modulated xenografted tumor progression by apoptosis induction. Our findings emphasize that CoQ0 triggered ROS-mediated apoptosis and cytoprotective autophagy.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2017-08-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: The dermato-protective effects of lucidone from Lindera erythrocarpa through the induction of Nrf2-mediated antioxidant genes in UVA-irradiated human skin keratinocytes

Hseu, You-Cheng / Chi-Rei Wu / Hsin-Ling Yang / Li-Sung Hsu / Mallikarjuna Korivi / Pei-Jane Huang / Show-Huei Chang / Ting-Tsz Ou / Yu-Cheng Tsai

Journal of functional foods. 2015 Jan., v. 12

2015

Abstract: This study investigated the dermato-protective effects of lucidone, a cyclopentenedione from Lindera erythrocarpa dried fruits, against UVA-induced oxidative stress and apoptosis in human keratinocytes (HaCaT). Pre-treatment with lucidone (1â4âÂµM) ... ...

Abstract	This study investigated the dermato-protective effects of lucidone, a cyclopentenedione from Lindera erythrocarpa dried fruits, against UVA-induced oxidative stress and apoptosis in human keratinocytes (HaCaT). Pre-treatment with lucidone (1â4âÂµM) significantly protects keratinocytes from UVA (15âJ/cm2)-induced cell death, excessive ROS generation, LDH release, lipid peroxidation, and DNA damage. Notably, lucidone inhibited the UVA-induced apoptosis of HaCaT cells as measured by a reduction of DNA fragmentation, mitochondria dysfunction, and Bcl-2/Bax dysregulation. Furthermore, antioxidant potential of lucidone was directly correlated with the induction of antioxidant genes, including HO-1, NQO-1, and Î³-GCLC by transcriptional activation of Nrf2. Treatment with hydrogen peroxide showed cellular byproducts of UVA irradiation-induced cell death, LDH release, and ROS generation, but these phenomena were inhibited by pre-treatment of lucidone. Besides, in Nrf2 knock-down cells, lucidone failed to protect UVA-induced oxidative stress or cell death. Our findings suggest that lucidone is capable of protecting skin cells from UVA-irradiated damage.
Keywords	antioxidant activity ; antioxidants ; apoptosis ; byproducts ; DNA damage ; DNA fragmentation ; dried fruit ; genes ; humans ; hydrogen peroxide ; keratinocytes ; Lindera erythrocarpa ; lipid peroxidation ; mitochondria ; oxidative stress ; transcriptional activation
Language	English
Dates of publication	2015-01
Size	p. 303-318.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2511964-3
ISSN	1756-4646
ISSN	1756-4646
DOI	10.1016/j.jff.2014.10.019
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The Antitumor Activity of Antrodia camphorata in Melanoma Cells

You-Cheng Hseu / Hsiao-Tung Tsou / K. J. Senthil Kumar / Kai-Yuan Lin / Hsueh-Wei Chang / Hsin-Ling Yang

Evidence-Based Complementary and Alternative Medicine, Vol

Modulation of Wnt/β-Catenin Signaling Pathways

2012 Volume 2012

Abstract: Antrodia camphorata (AC) is well known in Taiwan as a traditional Chinese medicine. The aim of this study was to investigate whether a fermented culture broth of AC could inhibit melanoma proliferation and progression via suppression of the Wnt/β-catenin ...

Abstract	Antrodia camphorata (AC) is well known in Taiwan as a traditional Chinese medicine. The aim of this study was to investigate whether a fermented culture broth of AC could inhibit melanoma proliferation and progression via suppression of the Wnt/β-catenin signaling pathway. In this study, we observed that AC treatment resulted in decreased cell viability and disturbed Wnt/β-catenin cascade in B16F10 and/or B16F1 melanoma cells. This result was accompanied by a decrease in the expression of Wnt/β-catenin transcriptional targets, including c-Myc and survivin. Furthermore, treatment of melanoma cells with AC resulted in a significant increase in apoptosis, which was associated with DNA fragmentation, cytochrome c release, caspase-9 and -3 activation, PARP degradation, Bcl-2/Bax dysregulation, and p53 expression. We also observed that AC caused G1 phase arrest mediated by a downregulation of cyclin D1 and CDK4 and increased p21 and p27 expression. In addition, we demonstrated that non- and subcytotoxic concentrations of AC markedly inhibited migration and invasion of highly metastatic B16F10 cells. The antimetastatic effect of AC was further confirmed by reductions in the levels of MMP-2, MMP-9, and VEGF expression. These results suggest that Antrodia camphorata may exert antitumor activity by downregulating the Wnt/β-catenin pathways.
Keywords	Other systems of medicine ; RZ201-999
Subject code	570
Language	English
Publishing date	2012-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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