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Article: In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.).

Stewart, J M / Hsu, C L

2014 Volume 137, Issue 2, Page(s) 113–117

Abstract: A convenient and reliable method for culturing cotton embryos is needed to obtain interspecific hybrids of this genus. C.A. Beasley and I.P. Ting (Amer. J. Bot. 60, 130, 1973) developed a phytohormone-supplemented medium (BTP) upon which the growth of ... ...

Abstract	A convenient and reliable method for culturing cotton embryos is needed to obtain interspecific hybrids of this genus. C.A. Beasley and I.P. Ting (Amer. J. Bot. 60, 130, 1973) developed a phytohormone-supplemented medium (BTP) upon which the growth of ovules was similar that of in situ ovules. This medium was examined for in-ovulo embryo culture. Although good ovule growth occurred on BTP no embryos developed to maturity. However, when the medium was supplemented with NH 4 (+) , more than 50% of the ovules produced mature embryos, and many of these germinated precociously after 8-10 weeks of culture. After germination seedlings were established on a separate medium designed to give balanced root and shoot growth. Subsequently young plants could be transferred to pots for greenhouse culture.
Language	English
Publishing date	2014-01-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	208909-9
ISSN	1432-2048 ; 0032-0935 ; 1866-2749
ISSN (online)	1432-2048
ISSN	0032-0935 ; 1866-2749
DOI	10.1007/BF00387547
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Article: A new protocol for isolation of mitochondrial DNA from cotton seedlings.

Hsu, C L / Mullin, B C

Plant cell reports

2013 Volume 7, Issue 5, Page(s) 356–360

Abstract: A procedure to isolate mtDNA from cotton seedlings G. hirsutum and G. barbadense has been developed. The new protocol allows for the isolation of cotton mtDNA of high purity, yield and digestibility by restriction endonucleases. The success of the ... ...

Abstract	A procedure to isolate mtDNA from cotton seedlings G. hirsutum and G. barbadense has been developed. The new protocol allows for the isolation of cotton mtDNA of high purity, yield and digestibility by restriction endonucleases. The success of the protocol is based on critical adjustments in the ionic strength of the homogenizing medium and washing buffer, the speed of grinding during homogenization, and the methods used for lysis of the mitochondria.
Language	English
Publishing date	2013-11-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	8397-5
ISSN	1432-203X ; 0721-7714 ; 0721-085X
ISSN (online)	1432-203X
ISSN	0721-7714 ; 0721-085X
DOI	10.1007/BF00269937
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Article: A fixed denture for a child with epidermolysis bullosa simplex.

Chuang, L C / Hsu, C L / Lin, S Y

European journal of paediatric dentistry

2015 Volume 16, Issue 4, Page(s) 315–318

Abstract: Aim: To report the caries treatment and delivery of a fixed denture for a 3-year-old girl with epidermolysis bullosa simplex (EBS).: Case report: EBS is manifested on the skin or mucous membranes where skin separation is easily induced by trauma. ... ...

Abstract	Aim: To report the caries treatment and delivery of a fixed denture for a 3-year-old girl with epidermolysis bullosa simplex (EBS). Case report: EBS is manifested on the skin or mucous membranes where skin separation is easily induced by trauma. Full- mouth rehabilitation under in-patient general anaesthesia was performed to the patient in conjunction with proper pre- and postoperative care. A fixed denture was fabricated and installed to replace the extracted teeth without later causing irritation on the mucosa. The prosthesis restored aesthetics and provided comfort without imposing the burden of compliance on the patient. Conclusion: Aided by meticulous pre- and postoperative care and oral hygiene reinforcement, comprehensive dental treatment coupled with fixed denture delivery can greatly improve the life quality and aesthetics for children with EBS.
MeSH term(s)	Child, Preschool ; Denture, Partial, Fixed ; Epidermolysis Bullosa Simplex/physiopathology ; Female ; Humans
Language	English
Publishing date	2015-11-18
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2035588-9
ISSN	2035-648X ; 1591-996X
ISSN (online)	2035-648X
ISSN	1591-996X
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Article ; Online: Movement of entomophagous arthropods in agricultural landscapes: links to pest suppression.

Schellhorn, N A / Bianchi, F J J A / Hsu, C L

Annual review of entomology

2014 Volume 59, Page(s) 559–581

Abstract: Entomophagous arthropods can provide valuable biological control services, but they need to fulfill their life cycle in agricultural landscapes often dominated by ephemeral and disturbed habitats. In this environment, movement is critical to escape from ... ...

Abstract	Entomophagous arthropods can provide valuable biological control services, but they need to fulfill their life cycle in agricultural landscapes often dominated by ephemeral and disturbed habitats. In this environment, movement is critical to escape from disturbances and to find resources scattered in space and time. Despite considerable research effort in documenting species movement and spatial distribution patterns, the quantification of arthropod movement has been hampered by their small size and the variety of modes of movement that can result in redistribution at different spatial scales. In addition, insight into how movement influences in-field population processes and the associated biocontrol services is limited because emigration and immigration are often confounded with local-scale population processes. More detailed measurements of the habitat functionality and movement processes are needed to better understand the interactions between species movement traits, disturbances, the landscape context, and the potential for entomophagous arthropods to suppress economically important pests.
MeSH term(s)	Agriculture ; Animal Distribution ; Animals ; Arthropods/physiology ; Environment ; Food Chain ; Pest Control, Biological ; Predatory Behavior ; Seasons ; Terminology as Topic
Language	English
Publishing date	2014
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	207927-6
ISSN	1545-4487 ; 0066-4170
ISSN (online)	1545-4487
ISSN	0066-4170
DOI	10.1146/annurev-ento-011613-161952
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Article ; Online: Exercise, Processing Speed, and Subsequent Falls: A Secondary Analysis of a 12-Month Randomized Controlled Trial.

Liu-Ambrose, Teresa / Davis, Jennifer C / Falck, Ryan S / Best, John R / Dao, Elizabeth / Vesely, Kristin / Ghag, Cheyenne / Rosano, Caterina / Hsu, C L / Dian, Larry / Cook, Wendy / Madden, Kenneth M / Khan, Karim M

The journals of gerontology. Series A, Biological sciences and medical sciences

2020 Volume 76, Issue 4, Page(s) 675–682

Abstract: Background: Strength and balance retraining exercises reduce the rate of subsequent falls in community-dwelling older adults who have previously fallen. Exercise can also improve cognitive function, including processing speed. Given processing speed ... ...

Abstract	Background: Strength and balance retraining exercises reduce the rate of subsequent falls in community-dwelling older adults who have previously fallen. Exercise can also improve cognitive function, including processing speed. Given processing speed predicts subsequent falls, we aimed to determine whether improved processing speed mediated the effects of the Otago Exercise Program on the rate of subsequent: (i) total falls, (ii) non-injurious falls, (iii) moderate injurious falls, and (iv) serious injurious falls. Method: A secondary complete case analysis of a 12-month, single-blind, randomized clinical trial among 256 of 344 adults aged at least 70 years who fell in the previous 12 months. Participants were randomized 1:1 to receive usual care plus the Otago Exercise Program (n = 123) or usual care (n = 133), consisting of fall prevention care provided by a geriatrician. The primary outcome was self-reported number of falls over 12 months (ie, rate of falls). Processing speed was assessed at baseline and at 12 months by the Digit Symbol Substitution Test (DSST). Causal mediation analyses were conducted using quasi-Bayesian estimates and 95% confidence intervals. Results: Exercise significantly reduced the rate of subsequent moderate injurious falls (IRR = 0.49; 95% CI: 0.31, 0.77; p = .002) and improved processing speed (estimated mean difference: 1.16 points; 95% CI: 0.11, 2.21). Improved DSST mediated the effect of exercise on the rate of subsequent moderate injurious falls (estimate: -0.06; 95% CI: -0.15, -0.001; p = .036). Conclusion: Improved processing speed may be a mechanism by which exercise reduces subsequent moderate injurious falls in older adults who fell previously. Clinical trials registration number: ClinicalTrials.gov Protocol Registration System:NCT01029171: https://clinicaltrials.gov/ct2/show/NCT01029171NCT00323596: https://clinicaltrials.gov/ct2/show/NCT00323596.
MeSH term(s)	Accidental Falls/prevention & control ; Aged ; Cognition/physiology ; Exercise/psychology ; Exercise Therapy/methods ; Female ; Geriatric Assessment/methods ; Home Care Services ; Humans ; Male ; Neuropsychological Tests ; Outcome and Process Assessment, Health Care ; Postural Balance ; Reaction Time/physiology ; Single-Blind Method ; Trauma Severity Indices ; Wounds and Injuries/diagnosis ; Wounds and Injuries/etiology ; Wounds and Injuries/prevention & control
Language	English
Publishing date	2020-11-22
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ZDB-ID	1223643-3
ISSN	1758-535X ; 1079-5006
ISSN (online)	1758-535X
ISSN	1079-5006
DOI	10.1093/gerona/glaa239
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Article: Effects of different contacting pressure on the transfer function between finger photoplethysmographic and radial blood pressure waveforms.

Hsiu, H / Hsu, C L / Wu, T L

Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine

2011 Volume 225, Issue 6, Page(s) 575–583

Abstract: Background: The blood pressure (BP) waveform is suggested to reflect the whole-body blood supply distribution, but its non-invasive assessment is not sufficiently user friendly for practical applications. The present authors studied the correlation ... ...

Abstract	Background: The blood pressure (BP) waveform is suggested to reflect the whole-body blood supply distribution, but its non-invasive assessment is not sufficiently user friendly for practical applications. The present authors studied the correlation between BP and photoplethysmography (PPG) waveforms, with the aim of determining the optimal range for contact pressure stimulation (PS) to produce a reliable transfer function in their harmonic parameters. Methods: Finger PPG and radial-artery BP signals were measured simultaneously and noninvasively on healthy volunteers (n = 45). PS of 0-200 mmHg was applied to the finger, and 1 min data sequences were recorded. In frequency-domain analysis, linear regression was applied to the calculated amplitude ratios or the first five harmonics between BP and PPG waveforms. Results: In the 60 mmHg-PS group, the BP-PPG regression of amplitude ratios was highest, and the agreements between them were also the best verified by Bland-Altman analysis. Conclusion: In the present study, frequency-domain analysis was performed to study the correlation between BP and PPG waveforms. Differences in pressure-induced-vasodilation responses underlie the different BP-PPG waveform correlations obtained by applying different PSs. The non-invasively derived PPG parameters might help to provide an easier method to acquire the radial-artery BP waveform, and hence broaden the application of BP waveform analysis.
MeSH term(s)	Adult ; Algorithms ; Blood Pressure/physiology ; Blood Pressure Determination/methods ; Fingers/physiology ; Humans ; Linear Models ; Male ; Photoplethysmography/methods ; Radial Artery/physiology
Language	English
Publishing date	2011-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1065942-0
ISSN	2041-3033 ; 0954-4119 ; 0046-2039
ISSN (online)	2041-3033
ISSN	0954-4119 ; 0046-2039
DOI	10.1177/0954411910396288
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Article ; Online: GATA3 interacts with and stabilizes HIF-1α to enhance cancer cell invasiveness.

Lin, M-C / Lin, J-J / Hsu, C-L / Juan, H-F / Lou, P-J / Huang, M-C

Oncogene

2017 Volume 36, Issue 30, Page(s) 4243–4252

Abstract: GATA binding protein 3 (GATA3) is indispensable in development of human organs. However, the role of GATA3 in cancers remains elusive. Hypoxia inducible factor (HIF)-1 plays an important role in pathogenesis of human cancers. Regulation of HIF-1α ... ...

Abstract	GATA binding protein 3 (GATA3) is indispensable in development of human organs. However, the role of GATA3 in cancers remains elusive. Hypoxia inducible factor (HIF)-1 plays an important role in pathogenesis of human cancers. Regulation of HIF-1α degradation is orchestrated through collaboration of its interacting proteins. In this study, we discover that GATA3 is upregulated in head and neck squamous cell carcinoma (HNSCC) and is an independent predictor for poor disease-free survival. GATA3 promotes invasive behaviours of HNSCC and melanoma cells in vitro and in immunodeficient mice. Mechanistically, GATA3 physically associates with HIF-1α under hypoxia to inhibit ubiquitination and proteasomal degradation of HIF-1α, which is independent of HIF-1α prolyl hydroxylation. Chromatin immunoprecipitation assays show that the GATA3/HIF-1α complex binds to and regulates HIF-1 target genes, which is also supported by the microarray analysis. Notably, the GATA3-mediated invasiveness can be significantly reversed by HIF-1α knockdown, suggesting a critical role of HIF-1α in the underlying mechanism of GATA3-mediated effects. Our findings suggest that GATA3 stabilizes HIF-1α to enhance cancer invasiveness under hypoxia and support the GATA3/HIF-1α axis as a potential therapeutic target for cancer treatment.
MeSH term(s)	Animals ; Blotting, Western ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/pathology ; Cell Hypoxia ; Chromatin Immunoprecipitation ; Female ; GATA3 Transcription Factor/metabolism ; Gene Expression Regulation, Neoplastic/physiology ; Head and Neck Neoplasms/metabolism ; Head and Neck Neoplasms/pathology ; Heterografts ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Immunohistochemistry ; Immunoprecipitation ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Neoplasm Invasiveness/pathology ; Real-Time Polymerase Chain Reaction ; Squamous Cell Carcinoma of Head and Neck
Chemical Substances	GATA3 Transcription Factor ; GATA3 protein, human ; HIF1A protein, human ; Hypoxia-Inducible Factor 1, alpha Subunit
Language	English
Publishing date	2017-03-06
Publishing country	England
Document type	Journal Article
ZDB-ID	639046-8
ISSN	1476-5594 ; 0950-9232
ISSN (online)	1476-5594
ISSN	0950-9232
DOI	10.1038/onc.2017.8
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Article ; Online: GATA3 interacts with and stabilizes HIF-1α to enhance cancer cell invasiveness.

Lin, M-C / Lin, J-J / Hsu, C-L / Juan, H-F / Lou, P-J / Huang, M-C

Oncogene

2017 Volume 36, Issue 30, Page(s) 4380

Abstract: This corrects the article DOI: 10.1038/onc.2017.8. ...

Abstract	This corrects the article DOI: 10.1038/onc.2017.8.
Language	English
Publishing date	2017-06-12
Publishing country	England
Document type	Journal Article ; Published Erratum
ZDB-ID	639046-8
ISSN	1476-5594 ; 0950-9232
ISSN (online)	1476-5594
ISSN	0950-9232
DOI	10.1038/onc.2017.196
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Article: Present state and historical changes of trace metal pollution in Kaoping coastal sediments, southwestern Taiwan.

Hung, J-J / Hsu, C-L

Marine pollution bulletin

2004 Volume 49, Issue 11-12, Page(s) 986–998

Abstract: Surface and gravity-cored sediments were collected from the Kaoping coastal area off southwestern Taiwan to determine particle size, organic carbon, trace metal concentration and enrichment factor (EF), 210Pb dating, and 206Pb/207Pb ratio for determining ...

Abstract	Surface and gravity-cored sediments were collected from the Kaoping coastal area off southwestern Taiwan to determine particle size, organic carbon, trace metal concentration and enrichment factor (EF), 210Pb dating, and 206Pb/207Pb ratio for determining present and historical metal pollution. Surface distributions of trace metals ranged from 0.02 to 0.13 mg/kg for Cd (EF: 0.16-1.65), from 35 to 189 mg/kg for Cr (EF: 0.85-2.92), from 25 to 64 mg/kg for Ni (EF: 0.53-2.38), from 10 to 32 mg/kg for Pb (EF: 1.20-4.94), and from 29 to 129 mg/kg for Zn (EF: 1.18-3.50). Trace metal concentrations correlate closely with distributions of mud (<63 microm) and organic carbon which accumulate largely around river mouths and within the Kaoping Canyon. With the exception of Cd, Cr and Ni in certain areas with rather coarse sediments, metals were generally elevated above the baseline levels over the studied area. Metals were also relatively enriched in areas with high contents of mud and organic carbon. Sedimentation rates derived from the excess 210Pb data in core sediments sampled from the canyon illustrate metal pollution beginning around 1970 that is corresponding to the booming time of economic growth in Taiwan. Meanwhile, the status of Pb pollution in core sediments is verified by an inversed correlation between 206Pb/207Pb ratios and Pb concentrations. Anthropogenic Pb and other metals in the studied area were apparently derived primarily from the Kaoping River and accumulated around the river mouth and within the Kaoping Canyon. The Kaoping Canyon appears to act as a major sink for river borne trace metals.
MeSH term(s)	Carbon/analysis ; Environmental Monitoring/statistics & numerical data ; Geologic Sediments/analysis ; Lead Radioisotopes ; Mass Spectrometry ; Metals, Heavy/analysis ; Particle Size ; Spectrophotometry, Atomic ; Taiwan
Chemical Substances	Lead Radioisotopes ; Metals, Heavy ; Carbon (7440-44-0)
Language	English
Publishing date	2004-12
Publishing country	England
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2001296-2
ISSN	1879-3363 ; 0025-326X
ISSN (online)	1879-3363
ISSN	0025-326X
DOI	10.1016/j.marpolbul.2004.06.028
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Article: Edge effects in the directionally biased distribution of Choristoneura rosaceana (Lepidoptera: Tortricidae) in apple orchards.

Hsu, C L / Agnello, A M / Reissig, W H

Environmental entomology

2009 Volume 38, Issue 2, Page(s) 433–441

Abstract: Edge effect tests have been used in a number of studies on obliquebanded leafroller, Choristoneura rosaceana (Harris), to test for evidence of mated female immigration into pheromone-treated orchards. This type of test compares obliquebanded leafroller ... ...

Abstract	Edge effect tests have been used in a number of studies on obliquebanded leafroller, Choristoneura rosaceana (Harris), to test for evidence of mated female immigration into pheromone-treated orchards. This type of test compares obliquebanded leafroller presence or activity around the perimeter of an orchard against presence or activity in the interior. Higher numbers detected around the edges of an orchard would indicate higher levels of flight activity at the edge, a pattern that could be generated by high levels of immigration. Recent work has shown that the spatial distribution of recaptured obliquebanded leafroller adults released from a single location can be directionally biased, which could obscure the ability to detect an edge effect. To test this theory, data from an orchard study conducted in 1991 that found no significant edge effect was reanalyzed. When we accounted for the directional bias in the distribution of first-generation mated female moths, we found an edge effect with significantly more mated females captured in the edge traps than in the center or mid-interior traps. No edge effect was found when the directional bias was ignored. In addition, second-generation males and mated females both showed a significant edge effect that had not been detected in the original analysis, which had combined both first- and second-generation data.
MeSH term(s)	Animals ; Behavior, Animal/drug effects ; Female ; Male ; Malus ; Moths/drug effects ; Moths/physiology ; Pheromones/pharmacology ; Population Density ; Sex Factors ; Sexual Behavior, Animal
Chemical Substances	Pheromones
Language	English
Publishing date	2009-04-22
Publishing country	England
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ISSN	0046-225X
ISSN	0046-225X
DOI	10.1603/022.038.0217
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