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Article ; Online: Senescence-Mediated Redox Imbalance in Liver and Kidney: Antioxidant Rejuvenating Potential of Green Tea Extract.

Hsu, Yu-Wen / Chen, Wen-Kang / Tsai, Chia-Fang

International journal of environmental research and public health

2021 Volume 19, Issue 1

Abstract: This study investigates the catechin composition and protective effect of green tea extract on senescence-mediated redox imbalance in the livers and kidneys of aged mice. The results showed that the seven catechins in the green tea extract analyzed in ... ...

Abstract	This study investigates the catechin composition and protective effect of green tea extract on senescence-mediated redox imbalance in the livers and kidneys of aged mice. The results showed that the seven catechins in the green tea extract analyzed in this study could be completely separated within 30 min and the main components of catechins in green tea extract were EGCG, EGC and ECG. In terms of the anti-senescence effects of green tea extract, green tea extract supplementation at doses of 125, 625 and 1250 mg/kg for 4 weeks significantly alleviated the senescence-mediated redox imbalance, as exhibited from significantly (
MeSH term(s)	Animals ; Antioxidants/metabolism ; Kidney/metabolism ; Lipid Peroxidation ; Liver/metabolism ; Mice ; Oxidation-Reduction ; Oxidative Stress ; Plant Extracts/metabolism ; Plant Extracts/pharmacology ; Tea
Chemical Substances	Antioxidants ; Plant Extracts ; Tea
Language	English
Publishing date	2021-12-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph19010260
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Article ; Online: Protective Effects of Rosmarinic Acid against Selenite-Induced Cataract and Oxidative Damage in Rats.

Tsai, Chia-Fang / Wu, Jia-Ying / Hsu, Yu-Wen

International journal of medical sciences

2019 Volume 16, Issue 5, Page(s) 729–740

Abstract: Cataracts are the major cause of blindness and are associated with oxidative damage of the lens. In the present study, the aim was to evaluate the protective effects of rosmarinic acid on selenite-induced cataractogenesis in Sprague-Dawley rat pups. The ... ...

Abstract	Cataracts are the major cause of blindness and are associated with oxidative damage of the lens. In the present study, the aim was to evaluate the protective effects of rosmarinic acid on selenite-induced cataractogenesis in Sprague-Dawley rat pups. The animals were randomly divided into five groups, each of which consisted of 10 rat pups. Group I served as normal control (vehicle administration). For testing cataract induction, animals of Groups II, III, IV, and V were administered a single subcutaneous injection of sodium selenite (2.46 mg/kg body weight) on postpartum day 12. After sodium selenite intoxication, Group II served as control selenite. From the 11th day through the 17th day, Groups III-V received rosmarinic acid intraperitoneally at doses of 5, 10, and 50 mg/kg, respectively. On postpartum day 24, the rat pups were examined for cataract formation, and the lenses were isolated for further analysis of proteins and oxidative damage indicators. Selenite caused significant (
MeSH term(s)	Animals ; Antioxidants/chemistry ; Antioxidants/pharmacology ; Catalase/genetics ; Cataract/chemically induced ; Cataract/drug therapy ; Cataract/pathology ; Cinnamates/chemistry ; Cinnamates/pharmacology ; Depsides/chemistry ; Depsides/pharmacology ; Disease Models, Animal ; Gene Expression/drug effects ; Humans ; Lipid Peroxidation/drug effects ; Oxidative Stress/drug effects ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Rats ; Selenious Acid/toxicity ; Superoxide Dismutase/genetics ; Rosmarinic Acid
Chemical Substances	Antioxidants ; Cinnamates ; Depsides ; Plant Extracts ; Catalase (EC 1.11.1.6) ; Superoxide Dismutase (EC 1.15.1.1) ; Selenious Acid (F6A27P4Q4R)
Language	English
Publishing date	2019-05-10
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2151424-0
ISSN	1449-1907 ; 1449-1907
ISSN (online)	1449-1907
ISSN	1449-1907
DOI	10.7150/ijms.32222
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Article ; Online: Human rs75776403 polymorphism links differential phenotypic and clinical outcomes to a CLEC18A p.T151M-driven multiomics.

Hsu, Yu-Wen / Wong, Henry Sung-Ching / Huang, Wan-Chen / Yeh, Yi-Hung / Hsiao, Chwan-Deng / Chang, Wei-Chiao / Hsieh, Shie-Liang

Journal of biomedical science

2022 Volume 29, Issue 1, Page(s) 43

Abstract: Background: Human traits, diseases susceptibility, and clinical outcomes vary hugely among individuals. Despite a fundamental understanding of genetic (or environmental) contributions, the detailed mechanisms of how genetic variation impacts molecular ... ...

Abstract	Background: Human traits, diseases susceptibility, and clinical outcomes vary hugely among individuals. Despite a fundamental understanding of genetic (or environmental) contributions, the detailed mechanisms of how genetic variation impacts molecular or cellular behaviours of a gene, and subsequently leads to such variability remain poorly understood. Methods: Here, in addition to phenome-wide correlations, we leveraged multiomics to exploit mechanistic links, from genetic polymorphism to protein structural or functional changes and a cross-omics perturbation landscape of a germline variant. Results: We identified a missense cis-acting expression quantitative trait locus in CLEC18A (rs75776403) in which the altered residue (T Conclusions: Collectively, we uncovered genetic pleiotropy in human complex traits and diseases via CLEC18A rs75776403-regulated pathways.
MeSH term(s)	Alleles ; Genetic Pleiotropy ; Genome-Wide Association Study ; Humans ; Lectins, C-Type/genetics ; Phenotype ; Polymorphism, Genetic ; Polymorphism, Single Nucleotide
Chemical Substances	CLEC18A protein, human ; Lectins, C-Type
Language	English
Publishing date	2022-06-18
Publishing country	England
Document type	Journal Article
ZDB-ID	1193378-1
ISSN	1423-0127 ; 1021-7770
ISSN (online)	1423-0127
ISSN	1021-7770
DOI	10.1186/s12929-022-00822-1
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Zs.A 4037: Show issues

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Article ; Online: Functional correlations between CXCL10/IP10 gene polymorphisms and risk of Kawasaki disease.

Hsu, Yu-Wen / Lu, Hsing-Fang / Chou, Wan-Hsuan / Kuo, Ho-Chang / Chang, Wei-Chiao

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

2020 Volume 32, Issue 2, Page(s) 363–370

Abstract: Background: Kawasaki disease (KD) is an acute systemic vasculitis syndrome with unknown pathogen. The immune system has been suggested to involve in the pathogenesis in KD. IP10 is a chemoattractant for initiating T-cell activation. The aim of this ... ...

Abstract	Background: Kawasaki disease (KD) is an acute systemic vasculitis syndrome with unknown pathogen. The immune system has been suggested to involve in the pathogenesis in KD. IP10 is a chemoattractant for initiating T-cell activation. The aim of this study was to investigate the association between genetic polymorphisms of IP10 and KD. Methods: A total of 354 KD patients and 1,709 control subjects (709 subjects in cohort 1 and 1,000 subjects in cohort 2) were enrolled in this study. Four tagging single nucleotide polymorphisms (rs3921, rs4256246, rs4508917, and rs4386624) were chosen for genotyping. Results: Our results indicated that CC genotype of rs3921 and GG genotype of rs4386624 had higher frequency in KD patients compared to control. In addition, higher plasma IP10 level was observed in CC genotype of rs3921 than CG genotype and GG genotype. C/G haplotype carriers of rs3921/rs4386624 had 5.48-fold risk for KD compared to G/C haplotype carriers. Two-locus analysis further showed the combinatorial effects of rs3921 and rs4386624 in KD susceptibility. Conclusions: This study indicated the close correlation between IP10 and the risk of Kawasaki disease.
MeSH term(s)	Case-Control Studies ; Chemokine CXCL10/genetics ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Humans ; Mucocutaneous Lymph Node Syndrome/genetics ; Polymorphism, Single Nucleotide
Chemical Substances	CXCL10 protein, human ; Chemokine CXCL10
Language	English
Publishing date	2020-11-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1057059-7
ISSN	1399-3038 ; 0905-6157 ; 0906-5784
ISSN (online)	1399-3038
ISSN	0905-6157 ; 0906-5784
DOI	10.1111/pai.13381
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Zs.A 3096: Show issues

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Article: Germline Genetic Association between Stromal Interaction Molecule 1 (STIM1) and Clinical Outcomes in Breast Cancer Patients.

Huang, Chi-Cheng / Lin, Min-Rou / Yang, Yu-Chen / Hsu, Yu-Wen / Wong, Henry Sung-Ching / Chang, Wei-Chiao

Journal of personalized medicine

2020 Volume 10, Issue 4

Abstract: Among all cancers in women, breast cancer has the highest incidence. The mortality of breast cancer is highly associated with metastasis. Migration and malignant transformation of cancer cells have been reported to be modulated by store-operated calcium ( ...

Abstract	Among all cancers in women, breast cancer has the highest incidence. The mortality of breast cancer is highly associated with metastasis. Migration and malignant transformation of cancer cells have been reported to be modulated by store-operated calcium (SOC) channels, which control calcium signaling and cell proliferation pathways. Stromal interaction molecule 1 (
Language	English
Publishing date	2020-12-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662248-8
ISSN	2075-4426
ISSN	2075-4426
DOI	10.3390/jpm10040287
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Article ; Online: Sensitive Detection of 8-Nitroguanine in DNA by Chemical Derivatization Coupled with Online Solid-Phase Extraction LC-MS/MS.

Hu, Chiung-Wen / Chang, Yuan-Jhe / Chen, Jian-Lian / Hsu, Yu-Wen / Chao, Mu-Rong

Molecules (Basel, Switzerland)

2018 Volume 23, Issue 3

Abstract: 8-Nitroguanine (8-nitroG) is a major mutagenic nucleobase lesion generated by peroxynitrite during inflammation and has been used as a potential biomarker to evaluate inflammation-related carcinogenesis. Here, we present an online solid-phase extraction ( ...

Abstract	8-Nitroguanine (8-nitroG) is a major mutagenic nucleobase lesion generated by peroxynitrite during inflammation and has been used as a potential biomarker to evaluate inflammation-related carcinogenesis. Here, we present an online solid-phase extraction (SPE) LC-MS/MS method with 6-methoxy-2-naphthyl glyoxal hydrate (MTNG) derivatization for a sensitive and precise measurement of 8-nitroG in DNA. Derivatization optimization revealed that an excess of MTNG is required to achieve complete derivatization in DNA hydrolysates (MTNG: 8-nitroG molar ratio of 3740:1). The use of online SPE effectively avoided ion-source contamination from derivatization reagent by washing away all unreacted MTNG before column chromatography and the ionization process in mass spectrometry. With the use of isotope-labeled internal standard, the detection limit was as low as 0.015 nM. Inter- and intraday imprecision was <5.0%. This method was compared to a previous direct LC-MS/MS method without derivatization. The comparison showed an excellent fit and consistency, suggesting that the present method has satisfactory effectiveness and reliability for 8-nitroG analysis. This method was further applied to determine the 8-nitroG in human urine. 8-NitroG was not detectable using LC-MS/MS with derivatization, whereas a significant false-positive signal was detected without derivatization. It highlights the use of MTNG derivatization in 8-nitroG analysis for increasing the method specificity.
MeSH term(s)	Chromatography, Liquid ; DNA/analysis ; DNA/chemistry ; DNA/genetics ; DNA Damage ; Guanine/analogs & derivatives ; Guanine/analysis ; Guanine/chemistry ; Reproducibility of Results ; Sensitivity and Specificity ; Solid Phase Extraction ; Tandem Mass Spectrometry
Chemical Substances	8-nitroguanine ; Guanine (5Z93L87A1R) ; DNA (9007-49-2)
Language	English
Publishing date	2018-03-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules23030605
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Zs.MO 81: Show issues

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Article ; Online: Characterization of T-Cell Receptor Repertoire in Patients with Rheumatoid Arthritis Receiving Biologic Therapies.

Chang, Che-Mai / Hsu, Yu-Wen / Wong, Henry Sung-Ching / Wei, James Cheng-Chung / Liu, Xiao / Liao, Hsien-Tzung / Chang, Wei-Chiao

Disease markers

2019 Volume 2019, Page(s) 2364943

Abstract: Rheumatoid arthritis (RA) is a systematic autoimmune disease, predominantly causing chronic polyarticular inflammation and joint injury of patients. For the treatment of RA, biologic disease-modifying antirheumatic drugs (bDMARDs) have been used to ... ...

Abstract	Rheumatoid arthritis (RA) is a systematic autoimmune disease, predominantly causing chronic polyarticular inflammation and joint injury of patients. For the treatment of RA, biologic disease-modifying antirheumatic drugs (bDMARDs) have been used to reduce inflammation and to interfere with disease progression through targeting and mediating the immune system. Although the therapeutic effects of bDMARDs in RA patients have been widely reported, whether these drugs also play important roles in T-cell repertoire status is still unclear. We therefore designed the study to identify the role of T-cell repertoire profiles in RA patients with different types of bDMARD treatments. A high-throughput sequencing approach was applied to profile the T-cell receptor beta chain (TCRB) repertoire of circulating T lymphocytes in eight patients given adalimumab (anti-TNF-
MeSH term(s)	Adult ; Aged ; Antirheumatic Agents/therapeutic use ; Arthritis, Rheumatoid/drug therapy ; Arthritis, Rheumatoid/metabolism ; Arthritis, Rheumatoid/pathology ; Biological Therapy/methods ; Biomarkers/analysis ; Case-Control Studies ; Disease Progression ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Prognosis ; Receptors, Antigen, T-Cell/genetics ; Receptors, Antigen, T-Cell/metabolism ; Tumor Necrosis Factor-alpha/antagonists & inhibitors
Chemical Substances	Antirheumatic Agents ; Biomarkers ; Receptors, Antigen, T-Cell ; Tumor Necrosis Factor-alpha
Language	English
Publishing date	2019-07-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	604951-5
ISSN	1875-8630 ; 0278-0240
ISSN (online)	1875-8630
ISSN	0278-0240
DOI	10.1155/2019/2364943
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Zs.A 1856: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Einzelsignatur: Show issues

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Article ; Online: Endosomal TLR3 co-receptor CLEC18A enhances host immune response to viral infection.

Huang, Ya-Lang / Huang, Ming-Ting / Sung, Pei-Shan / Chou, Teh-Ying / Yang, Ruey-Bing / Yang, An-Suei / Yu, Chung-Ming / Hsu, Yu-Wen / Chang, Wei-Chiao / Hsieh, Shie-Liang

Communications biology

2021 Volume 4, Issue 1, Page(s) 229

Abstract: Human C-type lectin member 18A (CLEC18A) is ubiquitously expressed in human, and highest expression levels are found in human myeloid cells and liver. In contrast, mouse CLEC18A (mCLEC18A) is only expressed in brain, kidney and heart. However, the ... ...

Abstract	Human C-type lectin member 18A (CLEC18A) is ubiquitously expressed in human, and highest expression levels are found in human myeloid cells and liver. In contrast, mouse CLEC18A (mCLEC18A) is only expressed in brain, kidney and heart. However, the biological functions of CLEC18A are still unclear. We have shown that a single amino acid change (S
MeSH term(s)	Animals ; Animals, Genetically Modified ; Cytokines/metabolism ; Disease Models, Animal ; Dogs ; Endosomes/drug effects ; Endosomes/immunology ; Endosomes/metabolism ; Endosomes/virology ; HEK293 Cells ; Host-Pathogen Interactions ; Humans ; Inflammation Mediators/metabolism ; Influenza A Virus, H5N1 Subtype/immunology ; Influenza A Virus, H5N1 Subtype/pathogenicity ; Lectins, C-Type/agonists ; Lectins, C-Type/genetics ; Lectins, C-Type/metabolism ; Madin Darby Canine Kidney Cells ; Mice, Inbred C57BL ; Mutation ; Orthomyxoviridae Infections/immunology ; Orthomyxoviridae Infections/metabolism ; Orthomyxoviridae Infections/virology ; Poly I-C/pharmacology ; Signal Transduction ; Species Specificity ; Toll-Like Receptor 3/agonists ; Toll-Like Receptor 3/metabolism ; Mice
Chemical Substances	CLEC18A protein, human ; Clec18a protein, mouse ; Cytokines ; Inflammation Mediators ; Lectins, C-Type ; TLR3 protein, human ; TLR3 protein, mouse ; Toll-Like Receptor 3 ; Poly I-C (O84C90HH2L)
Language	English
Publishing date	2021-02-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-021-01745-7
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Article ; Online: HSPB1 rs2070804 polymorphism is associated with the depth of primary tumor.

Hung, Chin-Sheng / Huang, Chien-Yu / Hsu, Yu-Wen / Makondi, Precious Takondwa / Chang, Wei-Chiao / Chang, Yu-Jia / Wang, Jaw-Yuan / Wei, Po-Li

Journal of cellular biochemistry

2019 Volume 121, Issue 1, Page(s) 63–69

Abstract: Background: Colorectal cancer (CRC) is the third most common cancer in the world. Genome-wide association studies are a powerful method to analyze the status of single-nucleotide polymorphisms (SNPs) in specific genes. Heat shock proteins (HSPs) were ... ...

Abstract	Background: Colorectal cancer (CRC) is the third most common cancer in the world. Genome-wide association studies are a powerful method to analyze the status of single-nucleotide polymorphisms (SNPs) in specific genes. Heat shock proteins (HSPs) were found to be involved in the cancer progression and chemoresistance. However, there is still no further study about polymorphisms of HSP beta-1 (HSPB1) in colorectal cancer. We proposed the SNP of HSPB1 may be correlated with the progression and metastasis in colon cancer. Methods: We recruited 379 colorectal cancer patients and categorized as four stages following the UICC TNM system. Then, we selected tagging SNPs of HSPB1 by 10% minimum allelic frequency in Han Chinese population from the HapMap database and analyze with the Chi-square test. Results: We demonstrated the association of HSPB1 genetic polymorphisms rs2070804 with tumor depth with colorectal cancer. But, there is a lack of association between HSPB1 genetic polymorphisms and colorectal cancer invasion, recurrence or metastasis. Conclusions: The polymorphisms of HSPB1 seemed to change the tumor behavior of colorectal cancer. HSPB1 rs2070804 polymorphism is associated with the depth of the primary tumor. But, there is no further correlation with other to the clinical parameters such as cancer invasiveness, local recurrence, or distant metastasis.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Alleles ; China ; Colorectal Neoplasms/genetics ; Disease Progression ; Drug Resistance, Neoplasm ; Female ; Genetic Predisposition to Disease ; Genome-Wide Association Study ; Genotype ; Heat-Shock Proteins/genetics ; Humans ; Male ; Middle Aged ; Molecular Chaperones/genetics ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasm Recurrence, Local ; Phenotype ; Polymorphism, Single Nucleotide ; Risk ; Young Adult
Chemical Substances	HSPB1 protein, human ; Heat-Shock Proteins ; Molecular Chaperones
Language	English
Publishing date	2019-07-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	392402-6
ISSN	1097-4644 ; 0730-2312
ISSN (online)	1097-4644
ISSN	0730-2312
DOI	10.1002/jcb.28266
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Zs.A 1114: Show issues

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Article ; Online: Inhibitory effects of rosmarinic acid on pterygium epithelial cells through redox imbalance and induction of extrinsic and intrinsic apoptosis.

Chen, Ya-Yu / Tsai, Chia-Fang / Tsai, Ming-Chu / Hsu, Yu-Wen / Lu, Fung-Jou

Experimental eye research

2017 Volume 160, Page(s) 96–105

Abstract: Pterygium is a common tumor-like ocular disease, which may be related to exposure to chronic ultraviolet (UV) radiation. Although the standard treatment for pterygium is surgical intervention, the recurrence rate of pterygium is high when no effective ... ...

Abstract	Pterygium is a common tumor-like ocular disease, which may be related to exposure to chronic ultraviolet (UV) radiation. Although the standard treatment for pterygium is surgical intervention, the recurrence rate of pterygium is high when no effective inhibitory drug is used after surgery. Rosmarinic acid (RA) is a polyphenol antioxidant with many biological activities, including anti-UV and anti-tumor properties. This study aimed to examine the inhibitory effects of RA on pterygium epithelial cells (PECs). Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was used to examine the cell cytotoxicity of PECs after RA treatment. A fluorescent probe, DCFH-DA (2',7'-dichlorofluorescin diacetate), was stained with PECs to measure intracellular reactive oxygen species (ROS) levels. Antioxidant activity assays were used to measure the levels of superoxide dismutase (SOD) and catalase (CAT) in PECs. Western blot analysis was used to determine the protein expression of nuclear factor E2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), quinone acceptor oxidoreductase 1 (NQO1), and apoptosis-associated proteins. RA significantly reduced the cell viability of the PECs. Treatment with RA remarkably increased the Nrf2 protein expression levels in the nucleus, HO-1 and NQO1 protein expression levels, and the activities of SOD and CAT. As a result, intracellular ROS levels in PECs were decreased. Additionally, the induction of extrinsic apoptosis on PECs by RA was associated with increasing expressions levels of Fas, Fas-associated protein with death domain (FADD), tumor necrosis factor-alpha (TNF-α), and caspase 8 protein. Moreover, the induction of intrinsic apoptotic cell death in PECs was confirmed through upregulation of cytochrome c, Bax, caspase 9, and caspase 3 and downregulation of Bcl-2 and pro-caspase 3. Our study demonstrated that RA could inhibit the viability of PECs through regulation of extrinsic and intrinsic apoptosis pathways. Therefore, RA may have potential as a therapeutic medication for pterygium.
MeSH term(s)	Antioxidants/pharmacology ; Apoptosis/drug effects ; Blotting, Western ; Cell Survival ; Cells, Cultured ; Cinnamates/pharmacology ; Complement C3-C5 Convertases ; Depsides/pharmacology ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Epithelial Cells/pathology ; Humans ; Oxidation-Reduction ; Pterygium/drug therapy ; Pterygium/metabolism ; Pterygium/pathology ; Reactive Oxygen Species/metabolism ; Signal Transduction ; Superoxide Dismutase/metabolism ; Rosmarinic Acid
Chemical Substances	Antioxidants ; Cinnamates ; Depsides ; Reactive Oxygen Species ; Superoxide Dismutase (EC 1.15.1.1) ; Complement C3-C5 Convertases (EC 3.4.21.-)
Language	English
Publishing date	2017-05-27
Publishing country	England
Document type	Journal Article
ZDB-ID	80122-7
ISSN	1096-0007 ; 0014-4835
ISSN (online)	1096-0007
ISSN	0014-4835
DOI	10.1016/j.exer.2017.05.008
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