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  1. Article ; Online: MiR-488-3p facilitates wound healing through CYP1B1-mediated Wnt/β-catenin signaling pathway by targeting MeCP2.

    Zuo, Chenchen / Fan, Pengju / Yang, Ying / Hu, Chengjun

    Journal of diabetes investigation

    2023  Volume 15, Issue 2, Page(s) 145–158

    Abstract: Introduction: Diabetic wounds are difficult to heal, but the pathogenesis is unknown. MicroRNAs (miRNAs) are thought to play important roles in wound healing. The effect of miR-488-3p in wound healing was studied in this article.: Materials and ... ...

    Abstract Introduction: Diabetic wounds are difficult to heal, but the pathogenesis is unknown. MicroRNAs (miRNAs) are thought to play important roles in wound healing. The effect of miR-488-3p in wound healing was studied in this article.
    Materials and methods: The gene methylation was measured by methylation specific PCR (MSP) assay. A dual-luciferase reporter assay was adopted to analyze the interaction between miR-488-3p and MeCP2.
    Results: Cytochrome P450 1B1 (CYP1B1) is a monooxygenase belonging to the cytochrome P450 family that aids in wound healing. Our findings showed that the miR-488-3p and CYP1B1 expression levels were much lower in wound tissues of diabetics with skin defects, but the methyl-CpG-binding protein 2 (MeCP2) level was significantly higher than that in control skin tissues. MiR-488-3p overexpression increased cell proliferation and migration, as well as HUVEC angiogenesis, while inhibiting apoptosis, according to function experiments. In vitro, MeCP2 inhibited wound healing by acting as a target of miR-488-3p. We later discovered that MeCP2 inhibited CYP1B1 expression by enhancing its methylation state. In addition, CYP1B1 knockdown inhibited wound healing. Furthermore, MeCP2 overexpression abolished the promoting effect of miR-488-3p on wound healing. It also turned out that CYP1B1 promoted wound healing by activating the Wnt4/β-catenin pathway. Animal experiments also showed that miR-488-3p overexpression could accelerate wound healing in diabetic male SD rats.
    Conclusions: MiR-488-3p is a potential therapeutic target for diabetic wound healing since it improved wound healing by activating the CYP1B1-mediated Wnt4/-catenin signaling cascade via MeCP2.
    MeSH term(s) Animals ; Male ; Rats ; Cell Line, Tumor ; Cell Proliferation/genetics ; Cytochrome P-450 Enzyme System/metabolism ; Diabetes Mellitus ; Methyl-CpG-Binding Protein 2/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Rats, Sprague-Dawley ; Wnt Signaling Pathway/genetics ; Wound Healing
    Chemical Substances Cytochrome P-450 Enzyme System (9035-51-2) ; Methyl-CpG-Binding Protein 2 ; MicroRNAs ; Mecp2 protein, rat ; MIRN488 microRNA, rat ; Cyp1b1 protein, rat (EC 1.14.14.1)
    Language English
    Publishing date 2023-11-13
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2625840-7
    ISSN 2040-1124 ; 2040-1116
    ISSN (online) 2040-1124
    ISSN 2040-1116
    DOI 10.1111/jdi.14099
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Maternal Supplementation with Ornithine Promotes Placental Angiogenesis and Improves Intestinal Development of Suckling Piglets.

    Yang, Yun / Hou, Guanyu / Ji, Fengjie / Zhou, Hanlin / Lv, Renlong / Hu, Chengjun

    Animals : an open access journal from MDPI

    2024  Volume 14, Issue 5

    Abstract: The blood vessels of the placenta are crucial for fetal growth. Here, lower vessel density and ornithine (Orn) content were observed in placentae for low-birth-weight fetuses versus normal-birth-weight fetuses at day 75 of gestation. Furthermore, the Orn ...

    Abstract The blood vessels of the placenta are crucial for fetal growth. Here, lower vessel density and ornithine (Orn) content were observed in placentae for low-birth-weight fetuses versus normal-birth-weight fetuses at day 75 of gestation. Furthermore, the Orn content in placentae decreased from day 75 to 110 of gestation. To investigate the role of Orn in placental angiogenesis, 48 gilts (Bama pig) were allocated into four groups. The gilts in the control group were fed a basal diet (CON group), while those in the experimental groups were fed a basal diet supplemented with 0.05% Orn (0.05% Orn group), 0.10% Orn (0.10% Orn group), and 0.15% Orn (0.15% Orn group), respectively. The results showed that 0.15% Orn and 0.10% Orn groups exhibited increased birth weight of piglets compared with the CON group. Moreover, the 0.15% Orn group was higher than the CON group in the blood vessel densities of placenta. Mechanistically, Orn facilitated placental angiogenesis by regulating vascular endothelial growth factor-A (VEGF-A). Furthermore, maternal supplementation with 0.15% Orn during gestation increased the jejunal and ileal villi height and the concentrations of colonic propionate and butyrate in suckling piglets. Collectively, these results showed that maternal supplementation with Orn promotes placental angiogenesis and improves intestinal development of suckling piglets.
    Language English
    Publishing date 2024-02-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani14050689
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Efficacy and Safety of Photodynamic Therapy for the Treatment of Actinic Keratoses: A Meta-Analysis Update of Randomized Controlled Trials.

    Hu, Chengjun / Luo, Xiangyue / Jiang, Chufeng / Lei, Shaorong / Sun, Yuming

    Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.

    2023  Volume 49, Issue 6, Page(s) 544–551

    Abstract: Background: Actinic keratoses (AKs) are common and some may evolve into squamous cell carcinoma. Photodynamic therapy (PDT), imiquimod, cryotherapy, and other methods have been reported to have good effects. However, which treatment is the most ... ...

    Abstract Background: Actinic keratoses (AKs) are common and some may evolve into squamous cell carcinoma. Photodynamic therapy (PDT), imiquimod, cryotherapy, and other methods have been reported to have good effects. However, which treatment is the most effective with the best cosmetic results and fewest complications is uncertain.
    Objective: To evaluate which method has the best efficacy and cosmetic results with less adverse events and recurrence rate.
    Materials and methods: All relevant articles up to July 31, 2022 were searched from Cochrane, Embase, and PubMed databases. Extract and analyze the data of efficacy, cosmetic results, local reactions, and adverse effects.
    Results: Twenty-nine articles with 3,,850 participants and 24,747 lesions were included. Quality of evidence was generally high. The efficacy of PDT was better in complete response (CR) (lesions CR; risk ratio (RR) 1.87; 95% confidence interval (CI) 1.55-1.87/patient CR; RR 3.07; 95% CI 2.07-4.56), overall preference, and cosmetic results. The time cumulative meta-analysis showed that the curative effect was gradually increasing before 2004, and then gradually stabilizing. Two groups showed no statistically significant differences in recurrence.
    Conclusion: Compared with other methods, PDT is significantly more effective for AK with excellent cosmetic results and reversible adverse effects.
    MeSH term(s) Humans ; Keratosis, Actinic/drug therapy ; Photosensitizing Agents/adverse effects ; Aminolevulinic Acid/therapeutic use ; Photochemotherapy/adverse effects ; Photochemotherapy/methods ; Randomized Controlled Trials as Topic ; Treatment Outcome
    Chemical Substances Photosensitizing Agents ; Aminolevulinic Acid (88755TAZ87)
    Language English
    Publishing date 2023-04-26
    Publishing country United States
    Document type Meta-Analysis ; Journal Article
    ZDB-ID 1227586-4
    ISSN 1524-4725 ; 1076-0512
    ISSN (online) 1524-4725
    ISSN 1076-0512
    DOI 10.1097/DSS.0000000000003784
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Hemangioma Endothelial Cells and Hemangioma Stem Cells in Infantile Hemangioma.

    Sun, Yuming / Qiu, Fengzhen / Hu, Chengjun / Guo, Yu / Lei, Shaorong

    Annals of plastic surgery

    2022  Volume 88, Issue 2, Page(s) 244–249

    Abstract: Background: Hemangioma is one of the most common benign tumors in infants and young children. The 2 most important cells in the course of infantile hemangioma (IH) are hemangioma stem cells (HemSCs) and hemangioma endothelial cells (HemECs). Infantile ... ...

    Abstract Background: Hemangioma is one of the most common benign tumors in infants and young children. The 2 most important cells in the course of infantile hemangioma (IH) are hemangioma stem cells (HemSCs) and hemangioma endothelial cells (HemECs). Infantile hemangioma is characterized by massive proliferation of HemECs, but current studies indicate that HemSCs play an important role in pathogenesis of IH.
    Objective: This review aimed to identify molecules that influence HemSC differentiation and HemEC proliferation and apoptosis to help clarify the pathogenesis of IH and provide novel drug targets for the treatment of IH.
    Methods: Relevant basic science studies related to IH were identified by searching Google Scholar, Embase, PubMed, MEDLINE, and peer-reviewed journal articles.
    Result: Hemangioma stem cells can differentiate into HemECs, pericytes, and adipocytes. In the proliferating phase of IH, HemSCs mainly differentiate into HemECs and pericytes to promote angiogenesis. In the regressive phase, they mainly differentiate into adipocytes. Therefore, increasing the proportion of HemSCs differentiating into adipocytes, inhibiting the proliferation of HemECs, and promoting the apoptosis of HemECs can facilitate the regression of IH.
    MeSH term(s) Cell Proliferation ; Child, Preschool ; Endothelial Cells ; Hemangioma ; Hemangioma, Capillary ; Humans ; Stem Cells
    Language English
    Publishing date 2022-01-10
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 423835-7
    ISSN 1536-3708 ; 0148-7043
    ISSN (online) 1536-3708
    ISSN 0148-7043
    DOI 10.1097/SAP.0000000000002835
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: DUOX2-Induced Oxidative Stress Inhibits Intestinal Angiogenesis through MMP3 in a Low-Birth-Weight Piglet Model.

    Zou, Dongbin / Yang, Yun / Ji, Fengjie / Lv, Renlong / Xu, Tieshan / Hu, Chengjun

    Antioxidants (Basel, Switzerland)

    2023  Volume 12, Issue 10

    Abstract: Intestinal vessels play a critical role in nutrient absorption, whereas the effect and mechanism of low birth weight (LBW) on its formation remain unclear. Here, twenty newborn piglets were assigned to the control (CON) group (1162 ± 98 g) and LBW group ( ...

    Abstract Intestinal vessels play a critical role in nutrient absorption, whereas the effect and mechanism of low birth weight (LBW) on its formation remain unclear. Here, twenty newborn piglets were assigned to the control (CON) group (1162 ± 98 g) and LBW group (724 ± 31 g) according to their birth weight. Results showed that the villus height and the activity of maltase in the jejunum were lower in the LBW group than in the CON group. LBW group exhibited a higher oxidative stress level and impaired mitochondrial function in the jejunum and was lower than the CON group in the intestinal vascular density. To investigate the role of oxidative stress in intestinal angiogenesis, H
    Language English
    Publishing date 2023-09-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox12101800
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Intrauterine Growth Restriction Affects Colonic Barrier Function

    Xiong, Liang / Azad, Md Abul Kalam / Liu, Yang / Zhang, Wanghong / Zhu, Qian / Hu, Chengjun / You, Jinming / Kong, Xiangfeng

    Antioxidants (Basel, Switzerland)

    2024  Volume 13, Issue 3

    Abstract: Intrauterine growth restriction (IUGR) pigs are characterized by long-term growth failure, metabolic disorders, and intestinal microbiota imbalance. The characteristics of the negative effects of IUGR at different growth stages of pigs are still unclear. ...

    Abstract Intrauterine growth restriction (IUGR) pigs are characterized by long-term growth failure, metabolic disorders, and intestinal microbiota imbalance. The characteristics of the negative effects of IUGR at different growth stages of pigs are still unclear. Therefore, this study explored through multi-omics analyses whether the IUGR damages the intestinal barrier function and alters the colonization and metabolic profiles of the colonic microbiota in growing-finishing pigs. Seventy-two piglets (36 IUGR and 36 NBW) were allocated for this trial to analyze physiological and plasma biochemical parameters, as well as oxidative damage and inflammatory response in the colon. Moreover, the colonic microbiota communities and metabolome were examined using 16s rRNA sequencing and metabolomics technologies to reveal the intestinal characteristics of IUGR pigs at different growth stages (25, 50, and 100 kg). IUGR altered the concentrations of plasma glucose, total protein, triglycerides, and cholesterol. Colonic tight junction proteins were markedly inhibited by IUGR. IUGR decreased plasma T-AOC, SOD, and GSH levels and colonic
    Language English
    Publishing date 2024-02-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox13030283
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Secretion of IL-6 and IL-8 in the senescence of bone marrow mesenchymal stem cells is regulated by autophagy via FoxO3a.

    Zheng, Yong / Wu, Shangrong / Ke, Haiqiang / Peng, Shanshan / Hu, Chengjun

    Experimental gerontology

    2022  Volume 172, Page(s) 112062

    Abstract: Bone marrow mesenchymal stem cells (BMSCs) are widely used for therapeutic applications in tissue engineering and regenerative medicine. Nevertheless, the function of BMSCs is adversely affected by senescence. Thus, understanding the molecular mechanisms ...

    Abstract Bone marrow mesenchymal stem cells (BMSCs) are widely used for therapeutic applications in tissue engineering and regenerative medicine. Nevertheless, the function of BMSCs is adversely affected by senescence. Thus, understanding the molecular mechanisms that contribute to BMSC senescence is critical for the development of BMSC-based tissue engineering and regenerative medicine. In this study, senescent BMSCs were characterized with >80 % of BMSCs stained positive for SA-β-gal, increased expressions of senescence-related genes (p16
    MeSH term(s) Autophagy/physiology ; Bone Marrow Cells/metabolism ; Cellular Senescence/physiology ; Interleukin-6/metabolism ; Interleukin-8 ; Mesenchymal Stem Cells/physiology ; Forkhead Transcription Factors
    Chemical Substances Interleukin-6 ; Interleukin-8 ; Forkhead Transcription Factors
    Language English
    Publishing date 2022-12-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 390992-x
    ISSN 1873-6815 ; 0531-5565
    ISSN (online) 1873-6815
    ISSN 0531-5565
    DOI 10.1016/j.exger.2022.112062
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Putrescine promotes MMP9-induced angiogenesis in skeletal muscle through hydrogen peroxide/METTL3 pathway.

    Hu, Chengjun / Ji, Fengjie / Lv, Renlong / Zhou, Hanlin / Hou, Guanyu / Xu, Tieshan

    Free radical biology & medicine

    2023  Volume 212, Page(s) 433–447

    Abstract: Blood vessels play a crucial role in the development of skeletal muscle, ensuring the supply of nutrients and oxygen. Putrescine, an essential polyamine for eukaryotic cells, has an unclear impact on skeletal muscle angiogenesis. In this study, we ... ...

    Abstract Blood vessels play a crucial role in the development of skeletal muscle, ensuring the supply of nutrients and oxygen. Putrescine, an essential polyamine for eukaryotic cells, has an unclear impact on skeletal muscle angiogenesis. In this study, we observed lower vessel density and reduced putrescine level in the muscle of low-birth-weight piglet models, and identified a positive correlation between putrescine content and muscle vessel density. Furthermore, putrescine was found to promote angiogenesis in skeletal muscle both in vitro and in vivo by targeting matrix metalloproteinase 9 (MMP9). On a mechanistic level, putrescine augmented the expression of methyltransferase like 3 (METTL3) by attenuating hydrogen peroxide production, thereby increasing the level of N6-methyladenosine (m6A)-modified MMP9 mRNA. This m6A-modified MMP9 mRNA was subsequently recognized and bound by the YTH N6-methyladenosine RNA binding protein 1 (YTHDF1), enhancing the stability of MMP9 mRNA and its protein expression, consequently accelerating angiogenesis in skeletal muscle. In summary, our findings suggest that putrescine enhances MMP9-mediated angiogenesis in skeletal muscle via the hydrogen peroxide/METTL3 pathway.
    MeSH term(s) Animals ; Swine ; Methyltransferases/genetics ; Methyltransferases/metabolism ; Putrescine/pharmacology ; Hydrogen Peroxide ; Matrix Metalloproteinase 9/genetics ; Angiogenesis ; Muscle, Skeletal/metabolism ; RNA, Messenger/genetics
    Chemical Substances Methyltransferases (EC 2.1.1.-) ; Putrescine (V10TVZ52E4) ; Hydrogen Peroxide (BBX060AN9V) ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; RNA, Messenger
    Language English
    Publishing date 2023-12-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 807032-5
    ISSN 1873-4596 ; 0891-5849
    ISSN (online) 1873-4596
    ISSN 0891-5849
    DOI 10.1016/j.freeradbiomed.2023.12.041
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota.

    Hu, Chengjun / Yan, Yingli / Ji, Fengjie / Zhou, Hanlin

    Frontiers in cellular and infection microbiology

    2021  Volume 11, Page(s) 671347

    Abstract: Maternal obesity induces placental dysfunction and intestinal microbial dysbiosis. However, the associations between intestinal microbiota and placental dysfunction are still unclear. In the present study, a gilt model was used to investigate the role of ...

    Abstract Maternal obesity induces placental dysfunction and intestinal microbial dysbiosis. However, the associations between intestinal microbiota and placental dysfunction are still unclear. In the present study, a gilt model was used to investigate the role of maternal obesity on placental oxidative stress, mitochondrial function, and fecal microbiota composition, meanwhile identifying microbiota markers associated with placental oxidative stress. Twenty gilts were divided into two groups based on their backfat thickness on parturition day: namely Con group (average backfat thickness = 33 mm), and Obese group (average backfat thickness = 39 mm). The results showed that Obese group was lower than Con group in the birth weight of piglets. Compared with the Con group, the Obesity group exhibited an increased oxidative damage and inflammatory response in placenta, as evidenced by the increased concentrations of placental reactive oxygen species (ROS), protein carboxyl, and interleukin-6 (IL-6). Obesity group was lower than Con group in the concentrations of placental adenosine triphosphate, citrate synthase, and complex I activity. In addition, lower propionate level and Bacteroidetes abundance in feces were seen in the Obese Group. Furthermore, the concentrations of placental ROS, protein carboxyl, and IL-6 were positively correlated with the abundance of
    MeSH term(s) Animals ; Female ; Gastrointestinal Microbiome ; Humans ; Obesity ; Obesity, Maternal ; Oxidative Stress ; Placenta ; Pregnancy ; Swine
    Language English
    Publishing date 2021-08-23
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2021.671347
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Dynamic alteration in the gut microbiota and metabolome of Huanjiang mini-pigs during pregnancy

    Xie, Peifeng / Hu, Chengjun / Azad, Md. Abul Kalam / He, Qinghua / Zhu, Qian / Kong, Xiangfeng

    BMC Vet Res. 2022 Dec., v. 18, no. 1 p.385-385

    2022  

    Abstract: BACKGROUND: Maternal gut microbiota and metabolites are associated with their offspring’s health. Our previous study showed that maternal body fat percentage increased from days 45 to 110 of gestation in a Huanjiang mini-pig model. Thus, this study aimed ...

    Abstract BACKGROUND: Maternal gut microbiota and metabolites are associated with their offspring’s health. Our previous study showed that maternal body fat percentage increased from days 45 to 110 of gestation in a Huanjiang mini-pig model. Thus, this study aimed to investigate the changes in gut microbiota composition and microbial metabolite profile of sows from days 45 to 110 of gestation. RESULTS: Twenty-four Huanjiang mini-pigs with average body weight were assigned for sample collection during early- (day 45 of pregnancy), mid- (day 75 of pregnancy), and late-pregnancy (day 110 of pregnancy). The results showed that the relative abundances of Clostridium_sensu_stricto_1, Romboutsia, Turicibacter, and Streptococcus in jejunal contents were higher at day 110 than those at day 45 or 75 of gestation. In the ileum, the relative abundance of Streptococcus was higher (P < 0.05) at day 110 of gestation, as well as the metabolism function of the jejunal and ileal microbiota. The ileal butyrate and acetate concentrations were higher at days 45 and 110 of gestation, respectively. In the colon, the concentrations of cadaverine and spermine were higher (P < 0.05) at days 45 and 110 of gestation, respectively. Metabolomic analyses demonstrated that the metabolic pathways, including D-glutamine and D-glutamate metabolism, phenylalanine/tyrosine/tryptophan biosynthesis, and alanine/aspartate/glutamate metabolism changed during gestation. CONCLUSION: Collectively, our results showed that gut microbiota composition and microbial metabolites changed dramatically from early to late pregnancy in a Huanjiang mini-pig model. These findings will provide new targets in formulating maternal nutritional interventions to alleviate the adverse effects during pregnancy on offspring health outcomes.
    Keywords Streptococcus ; acetates ; alanine ; aspartic acid ; biosynthesis ; body fat ; body weight ; butyrates ; cadaverine ; colon ; glutamic acid ; ileum ; intestinal microorganisms ; jejunum ; metabolites ; metabolome ; metabolomics ; miniature swine ; models ; phenylalanine ; pregnancy ; progeny ; spermine ; tryptophan ; tyrosine
    Language English
    Dates of publication 2022-12
    Size p. 385.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2191675-5
    ISSN 1746-6148
    ISSN 1746-6148
    DOI 10.1186/s12917-022-03477-0
    Database NAL-Catalogue (AGRICOLA)

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