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Article ; Online: Flexible biopolymer-assisted 3D printed bioceramics scaffold with high shape adaptability.

Hu, Xiaoqing / Li, Shanshan / He, Zhichao / Li, Xiaoyun / Wang, Xiaoying

International journal of biological macromolecules

2024 Volume 265, Issue Pt 1, Page(s) 130919

Abstract: Bioceramics are widely used in bone tissue engineering, yet the inherent high brittleness and low ductility of the ceramics lead to poor machinability, which restricts their clinical applications. Here, a flexible and processable 3D printed bioceramic ... ...

Abstract	Bioceramics are widely used in bone tissue engineering, yet the inherent high brittleness and low ductility of the ceramics lead to poor machinability, which restricts their clinical applications. Here, a flexible and processable 3D printed bioceramic scaffold with high ceramic content (66.7 %) and shape fidelity (volume shrinkage rate < 5 %) was developed by freeze-thaw cycles, which was assisted by polyvinyl alcohol (PVA) and silk fibroin (SF). The hydrogen bonding between PVA imparted printability to the ceramic ink and enabled the subsequent formation of flexible scaffolds, which can be twisted, bend and cut to match bone defects. After adding SF, the printability of the inks and hydrophilicity of the scaffolds were enhanced, owing to the interactions between PVA and SF. Further, combined with the formation of β-sheet in SF, the scaffolds exhibited superior mechanical strength and excellent thermal stability, and can fully recover at 35 % compressive strain, which was breaking through the brittleness bottleneck of conventional ceramic scaffolds. Moreover, in vitro experiments showed excellent mineralization ability, osteogenic and angiogenic activities of the scaffolds, demonstrating its potential in bone regeneration. This initial study offers a promising personalized material for bone repair that can be used rapidly during surgery.
MeSH term(s)	Tissue Scaffolds ; Bone and Bones ; Osteogenesis ; Tissue Engineering ; Fibroins ; Polyvinyl Alcohol ; Printing, Three-Dimensional
Chemical Substances	Fibroins (9007-76-5) ; Polyvinyl Alcohol (9002-89-5)
Language	English
Publishing date	2024-03-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.130919
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Article ; Online: Self-regulated efficient production of L-threonine via an artificial quorum sensing system in engineered Escherichia coli.

Song, Jie / Zhuang, Miaomiao / Fang, Yu / Hu, Xiaoqing / Wang, Xiaoyuan

Microbiological research

2024 Volume 284, Page(s) 127720

Abstract: Imbalance in carbon flux distribution is one of the most important factors affecting the further increase in the yield of high value-added natural products in microbial metabolic engineering. Meanwhile, the most common inducible expression systems are ... ...

Abstract	Imbalance in carbon flux distribution is one of the most important factors affecting the further increase in the yield of high value-added natural products in microbial metabolic engineering. Meanwhile, the most common inducible expression systems are difficult to achieve industrial-scale production due to the addition of high-cost or toxic inducers during the fermentation process. Quorum sensing system, as a typical model for density-dependent induction of gene expression, has been widely applied in synthetic biology. However, there are currently few reports for efficient production of microbial natural products by using quorum sensing system to self-regulate carbon flux distribution. Here, we designed an artificial quorum sensing system to achieve efficient production of L-threonine in engineered Escherichia coli by altering the carbon flux distribution of the central metabolic pathways at specific periods. Under the combination of switch module and production module, the system was applied to divide the microbial fermentation process into two stages including growth and production, and improve the production of L-threonine by self-inducing the expression of pyruvate carboxylase and threonine extracellular transporter protease after a sufficient amount of cell growth. The final strain TWF106/pST1011, pST1042pr could produce 118.2 g/L L-threonine with a yield of 0.57 g/g glucose and a productivity of 2.46 g/(L· h). The establishment of this system has important guidance and application value for the production of other high value-added chemicals in microorganisms by self-regulation.
Language	English
Publishing date	2024-04-16
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1189614-0
ISSN	1618-0623 ; 0944-5013
ISSN (online)	1618-0623
ISSN	0944-5013
DOI	10.1016/j.micres.2024.127720
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Article ; Online: Multiple cardiac defects in a rare case of thoracoabdominal ectopia cordis.

Hu, Xiaoqing / Gao, Lang / Xie, Mingxing / Li, Yuman

Asian journal of surgery

2023 Volume 46, Issue 11, Page(s) 4789–4790

MeSH term(s)	Humans ; Ectopia Cordis ; Heart Defects, Congenital ; Abnormalities, Multiple
Language	English
Publishing date	2023-06-01
Publishing country	Netherlands
Document type	Case Reports ; Letter
ZDB-ID	1068461-x
ISSN	0219-3108 ; 1015-9584
ISSN (online)	0219-3108
ISSN	1015-9584
DOI	10.1016/j.asjsur.2023.05.098
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Article ; Online: A rare case of left atrial free-floating thrombus in rheumatic mitral valve stenosis.

Ji, Mengmeng / Hu, Xiaoqing / Xie, Mingxing / Li, Yuman

Asian journal of surgery

2023 Volume 46, Issue 10, Page(s) 4578–4579

MeSH term(s)	Humans ; Mitral Valve Stenosis/diagnostic imaging ; Mitral Valve Stenosis/etiology ; Mitral Valve Stenosis/surgery ; Thrombosis/diagnostic imaging ; Thrombosis/etiology ; Thrombosis/surgery ; Heart Atria/diagnostic imaging
Language	English
Publishing date	2023-05-12
Publishing country	Netherlands
Document type	Case Reports ; Letter
ZDB-ID	1068461-x
ISSN	0219-3108 ; 1015-9584
ISSN (online)	0219-3108
ISSN	1015-9584
DOI	10.1016/j.asjsur.2023.05.023
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Article ; Online: Double-orifice mitral valve and partial atrioventricular septal defect: A rare combination.

Gao, Lang / Hu, Xiaoqing / Xie, Mingxing / Li, Yuman

Asian journal of surgery

2023 Volume 47, Issue 2, Page(s) 1119–1120

MeSH term(s)	Humans ; Mitral Valve ; Heart Septal Defects, Ventricular ; Heart Septal Defects, Atrial
Language	English
Publishing date	2023-11-28
Publishing country	Netherlands
Document type	Letter
ZDB-ID	1068461-x
ISSN	0219-3108 ; 1015-9584
ISSN (online)	0219-3108
ISSN	1015-9584
DOI	10.1016/j.asjsur.2023.10.135
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Article ; Online: Prosocial behaviour reduces unwanted intrusions of experimental traumatic memories.

Varma, Mohith M / Hu, Xiaoqing

Behaviour research and therapy

2021 Volume 148, Page(s) 103998

Abstract: Following trauma exposure, people often experience involuntary intrusions of traumatic memories, i.e., flashbacks. How to reduce such unwanted intrusions attracts attention from basic and translational memory research, with a goal to safeguard mental ... ...

Abstract	Following trauma exposure, people often experience involuntary intrusions of traumatic memories, i.e., flashbacks. How to reduce such unwanted intrusions attracts attention from basic and translational memory research, with a goal to safeguard mental well-being and promote resilience. Here, based on prosocial behaviour's well-documented psychological benefits, we hypothesized that post-trauma prosocial behaviour would causally reduce trauma-related symptoms, including involuntary intrusions. To test this novel hypothesis, we conducted two pre-registered lab studies (N = 180) using trauma films to induce lab-analogue trauma exposure. Following trauma exposure, participants were randomly assigned to prosocial or non-prosocial conditions. Specifically, in the prosocial condition, participants donated money to their preferred charities. In the non-prosocial conditions, participants completed either a neutral, number judgement task (Experiment 1) or a proself task (Experiment 2). Participants completed a 1-week intrusion diary and Impact of Event Scale-Revised (IES-R), to assess frequencies of traumatic intrusions and post-trauma stress disorder symptoms, respectively. Results showed that compared to non-prosocial behaviour, prosocial engagement (i.e. performing charitable donations) reduced involuntary traumatic intrusions in both lab settings and in their daily life as evidenced by 1-week intrusion diaries. While exploratory mediation analyses suggested that intrusion reduction was partly driven by enhanced positive affect afforded by prosocial behaviour, future studies are required to illuminate the underlying mechanisms. To the extent that post-trauma prosociality alleviated trauma-related symptoms, future research is warranted to investigate how various forms of prosocial behaviour in naturalistic setting could promote resilience following trauma exposure.
MeSH term(s)	Altruism ; Humans ; Memory ; Mental Recall ; Motion Pictures ; Stress Disorders, Post-Traumatic/psychology
Language	English
Publishing date	2021-11-16
Publishing country	England
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ZDB-ID	211997-3
ISSN	1873-622X ; 0005-7967
ISSN (online)	1873-622X
ISSN	0005-7967
DOI	10.1016/j.brat.2021.103998
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Article ; Online: A systematic review of clinical efficacy of frozen-thawed embryos and fresh embryos in in-vitro fertilization cycles.

Wang, Cui-Hua / Hu, Xiao-Qing

Cryobiology

2021 Volume 100, Page(s) 19–25

Abstract: Background: Frozen-thawed embryo (FTE) and fresh embryo (FE) transfer are two common strategies in vitro fertilization (IVF), while the results and findings still vary among studies.: Methods: We searched multiple databases for relevant studies ... ...

Abstract	Background: Frozen-thawed embryo (FTE) and fresh embryo (FE) transfer are two common strategies in vitro fertilization (IVF), while the results and findings still vary among studies. Methods: We searched multiple databases for relevant studies comparing the clinical effects of FTE and FE. Meta-analyses were conducted with Review Manager 5.0 to assess the efficacy among included articles. We also analyzed the risk of bias for the reports. Results: Nine studies eventually met the inclusion criteria from 2010 to 2018, and 11396 patients were included. The meta-analyses indicated no significant difference in biochemical pregnancy, clinical pregnancy and ongoing pregnancy rates. Meanwhile, the implantation rate and live birth rate in frozen-thawed embryos were much higher than those of fresh embryo. The birth weight in the frozen-thawed ET group was greater than that of the FE group, and the low birth weight rate in FTE was lower than FE group. Conclusion: Our findings suggested a trend toward favouring frozen-thawed FTE might be a preferred transfer strategy for patients with IVF.
MeSH term(s)	Cryopreservation/methods ; Embryo Transfer ; Female ; Fertilization ; Fertilization in Vitro ; Humans ; Pregnancy ; Pregnancy Rate ; Retrospective Studies ; Treatment Outcome
Language	English
Publishing date	2021-04-16
Publishing country	Netherlands
Document type	Journal Article ; Systematic Review
ZDB-ID	80098-3
ISSN	1090-2392 ; 0011-2240
ISSN (online)	1090-2392
ISSN	0011-2240
DOI	10.1016/j.cryobiol.2021.04.004
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Article ; Online: Revealing How Polyvinyl Chloride Microplastic Physicochemically Affect the Anaerobic Digestion of Waste Activated Sludge.

Cheng, Yi / Hu, Xiaoqing / Gao, Yuxuan / Wang, Lei / Wang, Guoxiang

Bulletin of environmental contamination and toxicology

2023 Volume 112, Issue 1, Page(s) 5

Abstract: Recent studies have shown that the microplastics in waste activated sludge (WAS) can directly reduce the microbial activity and influence the performance of anaerobic digestion. Unfortunately, few studies paid attention on the interactions between WAS ... ...

Abstract	Recent studies have shown that the microplastics in waste activated sludge (WAS) can directly reduce the microbial activity and influence the performance of anaerobic digestion. Unfortunately, few studies paid attention on the interactions between WAS and MPs, since MPs could impact the contact between sludge flocs and microorganisms. We found that PVC-MP changed the interfacial energy properties of the WAS surface and affected methane production. Low concentration (40 mg/L) of PVC-MP changed the water affinity and greatly reduced the energy barrier of interfacial reaction. Simultaneously, WAS surface charge characteristics changed with increasing MPs concentration, which made the sludge difficult to contact with microorganisms. The change process of WAS surface functional groups also indicated that PVC-MP first cover the sludge surface to prevent from being utilized by microorganisms, and then affect the surface protein structure before toxic substances leaching. Our study provides new insights into how MPs affect anaerobic digestion.
MeSH term(s)	Sewage/chemistry ; Microplastics/metabolism ; Plastics ; Polyvinyl Chloride ; Anaerobiosis ; Waste Disposal, Fluid ; Bioreactors ; Methane
Chemical Substances	Sewage ; Microplastics ; Plastics ; Polyvinyl Chloride (9002-86-2) ; Methane (OP0UW79H66)
Language	English
Publishing date	2023-12-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	6895-0
ISSN	1432-0800 ; 0007-4861
ISSN (online)	1432-0800
ISSN	0007-4861
DOI	10.1007/s00128-023-03828-z
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Article: Adjustable extracellular matrix rigidity tumor model for studying stiffness dependent pancreatic ductal adenocarcinomas progression and tumor immunosuppression.

Zhang, Haoxiang / Chen, Jiaoshun / Hu, Xiaoqing / Bai, Jianwei / Yin, Tao

Bioengineering & translational medicine

2023 Volume 8, Issue 3, Page(s) e10518

Abstract: Pancreatic ductal adenocarcinomas (PDAC) is one of the stiffest malignancies with strong solid stresses. Increasing stiffness could alter cellular behavior and trigger internal signaling pathways and is strongly associated with a poor prognosis in PDAC. ... ...

Abstract	Pancreatic ductal adenocarcinomas (PDAC) is one of the stiffest malignancies with strong solid stresses. Increasing stiffness could alter cellular behavior and trigger internal signaling pathways and is strongly associated with a poor prognosis in PDAC. So far, there has been no report on of an experimental model that can rapidly construct and stably maintain a stiffness gradient dimension in both vitro and in vivo. In this study, a gelatin methacryloyl (GelMA)-based hydrogel was designed for in vitro and in vivo PDAC experiments. The GelMA-based hydrogel has porous, adjustable mechanical properties and excellent in vitro and in vivo biocompatibility. The GelMA-based in vitro 3D culture method can effectively form a gradient and stable extracellular matrix stiffness, affecting cell morphology, cytoskeleton remodeling, and malignant biological behaviors such as proliferation and metastasis. This model is suitable for in vivo studies with long-term maintenance of matrix stiffness and no significant toxicity. High matrix stiffness can significantly promote PDAC progression and tumor immunosuppression. This novel adaptive extracellular matrix rigidity tumor model is an excellent candidate for further development as an in vitro and in vivo biomechanical study model of PDAC or other tumors with strong solid stresses.
Language	English
Publishing date	2023-04-05
Publishing country	United States
Document type	Journal Article
ISSN	2380-6761
ISSN	2380-6761
DOI	10.1002/btm2.10518
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Article ; Online: Green supply chain game model and contract design: risk neutrality vs. risk aversion.

Cai, Jianhu / Lin, Huazhen / Hu, Xiaoqing / Ping, Minyan

Environmental science and pollution research international

2022 Volume 29, Issue 34, Page(s) 51871–51891

Abstract: This paper incorporates the players' risk attitudes into a green supply chain (GSC) consisting of a supplier and a retailer. The supplier conducts production and determines the green level and wholesale price as a game leader; the retailer sells green ... ...

Abstract	This paper incorporates the players' risk attitudes into a green supply chain (GSC) consisting of a supplier and a retailer. The supplier conducts production and determines the green level and wholesale price as a game leader; the retailer sells green products to consumers and determines the retail price as a follower. Equilibrium solutions are derived, and the influence of risk aversion on the GSC is examined. Our results show that, for the centralized GSC, risk aversion lowers the green level and the retail price, while for the decentralized GSC, risk aversion lowers the wholesale price and the retail price, but it may induce the supplier to increase the green level given a high-risk tolerance of the supplier. Meanwhile, the risk-averse decentralized GSC may obtain more expected profit than the risk-neutral decentralized GSC. Furthermore, this paper designs a revenue-and-cost-sharing joint contract to coordinate the risk-neutral GSC, and such a contract can improve the risk-averse GSC under specific conditions.
MeSH term(s)	Commerce ; Conservation of Natural Resources/economics ; Consumer Behavior ; Game Theory ; Risk
Language	English
Publishing date	2022-03-07
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-022-18804-z
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