Article ; Online: Circulating tumor DNA analysis for tumor diagnosis.
2021 Volume 228, Page(s) 122220
Abstract: Tumor is a kind of abnormal organism generated by the proliferation and differentiation of cells in the body under the action of various initiating and promoting factors, which seriously threatens human life and health. Tumorigenesis is a gradual process ...
| Abstract | Tumor is a kind of abnormal organism generated by the proliferation and differentiation of cells in the body under the action of various initiating and promoting factors, which seriously threatens human life and health. Tumorigenesis is a gradual process that involves multistage reactions and the accumulation of mutations. Gene mutation usually occurs during tumorigenesis, and can be used for tumor diagnosis. Early diagnosis is the most effective way to improve the cure rate and reduce the mortality rate. Among the peripheral blood circulating tumor DNA (ctDNA), gene mutation in keeping with tumor cells can be detected, which can potentially replace tumor tissue section for early diagnosis. It has been considered as a liquid biopsy marker with good clinical application prospect. However, the high fragmentation and low concentration of ctDNA in blood result in the difficulty of tumor stage determination. Therefore, high sensitive and specific mutation detection methods have been developed to detect trace mutant ctDNA. At present, the approaches include digital PCR (dPCR), Bead, Emulsion, Amplification and Magnetic (BEAMing), Next Generation Sequencing (NGS), Amplification Refractory Mutation System (ARMS), etc. In this paper, the principle, characteristics, latest progress and application prospects of these methods are reviewed, which will facilitate researchers to choose appropriate ctDNA detection approaches. |
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| MeSH term(s) | Biomarkers, Tumor/genetics ; Circulating Tumor DNA/genetics ; DNA, Neoplasm/genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Liquid Biopsy ; Mutation ; Neoplasms/diagnosis ; Neoplasms/genetics |
| Chemical Substances | Biomarkers, Tumor ; Circulating Tumor DNA ; DNA, Neoplasm |
| Language | English |
| Publishing date | 2021-02-19 |
| Publishing country | Netherlands |
| Document type | Journal Article ; Review |
| ZDB-ID | 1500969-5 |
| ISSN | 1873-3573 ; 0039-9140 |
| ISSN (online) | 1873-3573 |
| ISSN | 0039-9140 |
| DOI | 10.1016/j.talanta.2021.122220 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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