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Article ; Online: Early events in the signalling pathway for the activation of MAPKs in rice roots exposed to nickel.

Chen, Po-Yu / Huang, Tsai-Lien / Huang, Hao-Jen

2020 Volume 34, Issue 11, Page(s) 995–1001

Abstract: It is well known that small quantities of nickel (Ni) are essential for plant species, and higher concentrations of Ni retard plant growth. However, the molecular mechanisms responsible for the regulation of plant growth by Ni are not well understood. ... ...

Abstract	It is well known that small quantities of nickel (Ni) are essential for plant species, and higher concentrations of Ni retard plant growth. However, the molecular mechanisms responsible for the regulation of plant growth by Ni are not well understood. The aim of this study is to investigate the early signalling pathways activated by Ni on rice (Oryza sativa L.) root. We showed that Ni elicited a remarkable increase in myelin basic protein (MBP) kinase activities. By immunoblot and immunoprecipitation analyses, it is suggested that Ni-activated 40- and 42-kDa MBP kinases are mitogen-activated protein kinases (MAPKs). Pretreatment of rice roots with the antioxidant, glutathione (GSH), the phospholipase D (PLD) inhibitor, n-butanol, and the calmodulin and CDPK antagonist and W7 inhibited Ni-induced MAPK activation. These results suggest that various signalling components are involved in transduction of the Ni signal in rice roots.
Language	English
Publishing date	2020-07-21
Publishing country	Australia
Document type	Journal Article
ZDB-ID	2071582-1
ISSN	1445-4416 ; 1445-4408
ISSN (online)	1445-4416
ISSN	1445-4408
DOI	10.1071/FP07163
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Article ; Online: Volatile cinnamaldehyde induces systemic salt tolerance in the roots of rice (Oryza sativa).

Huang, Chung-Chih / Lee, Yun-Ting / Ly, Thach-Thao / Wang, Chong-Yue / Chang, Yao-Tsung / Hou, Ping-Fu / Liu, Zin-Huang / Huang, Hao-Jen

Physiologia plantarum

2023 Volume 175, Issue 3, Page(s) e13938

Abstract: Cinnamaldehyde (CA) is a volatile plant secondary metabolite that exhibits strong anti-pathogenic activities. Nonetheless, less is known about the effect of CA on plant tolerance to abiotic stresses. In this study, we delineated the effects of CA ... ...

Abstract	Cinnamaldehyde (CA) is a volatile plant secondary metabolite that exhibits strong anti-pathogenic activities. Nonetheless, less is known about the effect of CA on plant tolerance to abiotic stresses. In this study, we delineated the effects of CA fumigation on rice roots (Oryza Sativa L cv. TNG67) under salinity stress (200 mM NaCl). Our result showed that CA vapor significantly alleviated salinity-induced ROS accumulation and cell death. This CA-induced alleviation appears to be mediated primarily by the upregulation of proline metabolism genes, the rapid proline accumulation, and the decrease of Na
MeSH term(s)	Oryza/genetics ; Salt Tolerance ; Sodium Chloride/pharmacology ; Sodium Chloride/metabolism ; Antioxidants/metabolism ; Proline/metabolism ; Salinity
Chemical Substances	cinnamaldehyde (SR60A3XG0F) ; Sodium Chloride (451W47IQ8X) ; Antioxidants ; Proline (9DLQ4CIU6V)
Language	English
Publishing date	2023-04-28
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	2020837-6
ISSN	1399-3054 ; 0031-9317
ISSN (online)	1399-3054
ISSN	0031-9317
DOI	10.1111/ppl.13938
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Article ; Online: Volatile cinnamaldehyde induces systemic salt tolerance in the roots of rice (Oryza sativa)

Huang, Chung‐Chih / Lee, Yun‐Ting / Ly, Thach‐Thao / Wang, Chong‐Yue / Chang, Yao‐Tsung / Hou, Ping‐Fu / Liu, Zin‐Huang / Huang, Hao‐Jen

Physiologia Plantarum. 2023 , v. 175, no. 3 p.e13938-

2023

Abstract: Cinnamaldehyde (CA) is a volatile plant secondary metabolite that exhibits strong anti‐pathogenic activities. Nonetheless, less is known about the effect of CA on plant tolerance to abiotic stresses. In this study, we delineated the effects of CA ... ...

Abstract	Cinnamaldehyde (CA) is a volatile plant secondary metabolite that exhibits strong anti‐pathogenic activities. Nonetheless, less is known about the effect of CA on plant tolerance to abiotic stresses. In this study, we delineated the effects of CA fumigation on rice roots (Oryza Sativa L cv. TNG67) under salinity stress (200 mM NaCl). Our result showed that CA vapor significantly alleviated salinity‐induced ROS accumulation and cell death. This CA‐induced alleviation appears to be mediated primarily by the upregulation of proline metabolism genes, the rapid proline accumulation, and the decrease of Na⁺/K⁺ ratio as early as 3 h after NaCl treatment. Of note, the activities of peroxidase (POD; EC 1.11.1.7) isozymes a and b were decreased by CA fumigation, and the activities of catalase (CAT; EC 1.11.1.6) and superoxide dismutase (SOD; EC 1.15.1.1) were not significantly affected. Our findings suggest that CA vapor might be useful for priming rice roots to withstand salinity stress, which is more prevalent due to the ongoing global climate change. To the best of our knowledge, this is the first study to show modulation of macro‐ and micro‐elements as well as antioxidative factors after CA fumigation of salinity‐stressed rice roots.
Keywords	Oryza sativa ; catalase ; cell death ; climate change ; fumigation ; isozymes ; metabolism ; peroxidase ; proline ; rice ; salt stress ; salt tolerance ; secondary metabolites ; superoxide dismutase ; vapors
Language	English
Dates of publication	2023-05
Publishing place	Blackwell Publishing Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2020837-6
ISSN	1399-3054 ; 0031-9317
ISSN (online)	1399-3054
ISSN	0031-9317
DOI	10.1111/ppl.13938
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Article ; Online: Comparative study of two indoor microbial volatile pollutants, 2-Methyl-1-butanol and 3-Methyl-1-butanol, on growth and antioxidant system of rice (Oryza sativa) seedlings.

Nguyen, Diem-Kieu / Nguyen, Tri-Phuong / Li, Yi-Rong / Ohme-Takagi, Masaru / Liu, Zin-Huang / Ly, Thach-Thao / Nguyen, Van-Anh / Trinh, Ngoc-Nam / Huang, Hao-Jen

Ecotoxicology and environmental safety

2024 Volume 272, Page(s) 116055

Abstract: 2-Methyl-1-butanol (2MB) and 3-Methyl-1-butanol (3MB) are microbial volatile organic compounds (VOCs) and found in indoor air. Here, we applied rice as a bioindicator to investigate the effects of these indoor microbial volatile pollutants. A remarkable ... ...

Abstract	2-Methyl-1-butanol (2MB) and 3-Methyl-1-butanol (3MB) are microbial volatile organic compounds (VOCs) and found in indoor air. Here, we applied rice as a bioindicator to investigate the effects of these indoor microbial volatile pollutants. A remarkable decrease in germination percentage, shoot and root elongation, as well as lateral root numbers were observed in 3MB. Furthermore, ROS production increased by 2MB and 3MB, suggesting that pentanol isomers could induce cytotoxicity in rice seedlings. The enhancement of peroxidase (POD) and catalase (CAT) activity provided evidence that pentanol isomers activated the enzymatic antioxidant scavenging systems, with a more significant effect observed in 3MB. Furthermore, 3MB induced higher activity levels of glutathione (GSH), oxidized glutathione (GSSG), and the GSH/GSSG ratio in rice compared to the levels induced by 2MB. Additionally, qRT-PCR analysis showed more up-regulation in the expression of glutaredoxins (GRXs), peroxiredoxins (PRXs), thioredoxins (TRXs), and glutathione S-transferases (GSTUs) genes in 3MB. Taking the impacts of pentanol isomers together, the present study suggests that 3MB exhibits more cytotoxic than 2MB, as such has critical effects on germination and the early seedling stage of rice. Our results provide molecular insights into how isomeric indoor microbial volatile pollutants affect plant growth through airborne signals.
MeSH term(s)	Antioxidants/metabolism ; Seedlings ; Oryza/metabolism ; Pentanols/metabolism ; Pentanols/pharmacology ; 1-Butanol/metabolism ; 1-Butanol/pharmacology ; Environmental Pollutants/metabolism ; Glutathione Disulfide/metabolism ; Oxidative Stress ; Glutathione/metabolism ; Plant Roots/metabolism
Chemical Substances	Antioxidants ; isopentyl alcohol (DEM9NIT1J4) ; tert-amyl alcohol (69C393R11Z) ; Pentanols ; 1-Butanol (8PJ61P6TS3) ; Environmental Pollutants ; Glutathione Disulfide (ULW86O013H) ; Glutathione (GAN16C9B8O)
Language	English
Publishing date	2024-02-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	436536-7
ISSN	1090-2414 ; 0147-6513
ISSN (online)	1090-2414
ISSN	0147-6513
DOI	10.1016/j.ecoenv.2024.116055
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Article ; Online: Signaling pathways involved in microbial indoor air pollutant 3-methyl-1-butanol in the induction of stomatal closure in Arabidopsis.

Truong, Tu-Trinh Thi / Chiu, Chi-Chou / Su, Pei-Yu / Chen, Jing-Yu / Nguyen, Tri-Phuong / Ohme-Takagi, Masaru / Lee, Ruey-Hua / Cheng, Wan-Hsing / Huang, Hao-Jen

Environmental science and pollution research international

2024 Volume 31, Issue 5, Page(s) 7556–7568

Abstract: Indoor air pollution is a global problem and one of the main stress factors that has negative effects on plant and human health. 3-methyl-1-butanol (3MB), an indoor air pollutant, is a microbial volatile organic compound (mVOC) commonly found in damp ... ...

Abstract	Indoor air pollution is a global problem and one of the main stress factors that has negative effects on plant and human health. 3-methyl-1-butanol (3MB), an indoor air pollutant, is a microbial volatile organic compound (mVOC) commonly found in damp indoor dwellings. In this study, we reported that 1 mg/L of 3MB can elicit a significant reduction in the stomatal aperture ratio in Arabidopsis and tobacco. Our results also showed that 3MB enhances the reactive oxygen species (ROS) production in guard cells of wild-type Arabidopsis after 24 h exposure. Further investigation of 24 h 3MB fumigation of rbohD, the1-1, mkk1, mkk3, and nced3 mutants revealed that ROS production, cell wall integrity, MAPK kinases cascade, and phytohormone abscisic acid are all involved in the process of 3MB-induced stomatal. Our findings proposed a mechanism by which 3MB regulates stomatal closure in Arabidopsis. Understanding the mechanisms by which microbial indoor air pollutant induces stomatal closure is critical for modulating the intake of harmful gases from indoor environments into leaves. Investigations into how stomata respond to the indoor mVOC 3MB will shed light on the plant's "self-defense" system responding to indoor air pollution.
MeSH term(s)	Humans ; Arabidopsis/metabolism ; Arabidopsis Proteins/metabolism ; Reactive Oxygen Species/metabolism ; Plant Stomata ; Signal Transduction ; Abscisic Acid/metabolism ; Pentanols
Chemical Substances	isopentyl alcohol (DEM9NIT1J4) ; Arabidopsis Proteins ; Reactive Oxygen Species ; tert-amyl alcohol (69C393R11Z) ; Abscisic Acid (72S9A8J5GW) ; Pentanols
Language	English
Publishing date	2024-01-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-023-31641-y
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Article ; Online: The involvement of AtMKK1 and AtMKK3 in plant-deleterious microbial volatile compounds-induced defense responses

Zhang, Jinghan / Wang, Wu-Guei / Su, Pei-Yu / Chen, Yu-Shuo / Nguyen-Tri, Phuong / Xu, Jian / Ohme-Takagi, Masaru / Mimura, Tetsuro / Hou, Ping-Fu / Huang, Hao-Jen

Plant Mol Biol. 2023 Jan., v. 111, no. 1-2 p.21-36

2023

Abstract: KEY MESSAGE: Plant-deleterious microbial volatiles activate the transactivation of hypoxia, MAMPs and wound responsive genes in Arabidopsis thaliana. AtMKK1 and AtMKK3 are involved in the plant-deleterious microbial volatiles-induced defense responses. ... ...

Abstract	KEY MESSAGE: Plant-deleterious microbial volatiles activate the transactivation of hypoxia, MAMPs and wound responsive genes in Arabidopsis thaliana. AtMKK1 and AtMKK3 are involved in the plant-deleterious microbial volatiles-induced defense responses. Microbial volatile compounds (mVCs) are a collection of volatile metabolites from microorganisms with biological effects on all living organisms. mVCs function as gaseous modulators of plant growth and plant health. In this study, the defense events induced by plant-deleterious mVCs were investigated. Enterobacter aerogenes VCs lead to growth inhibition and immune responses in Arabidopsis thaliana. E. aerogenes VCs negatively regulate auxin response and transport gene expression in the root tip, as evidenced by decreased expression of DR5::GFP, PIN3::PIN3-GFP and PIN4::PIN4-GFP. Data from transcriptional analysis suggests that E. aerogenes VCs trigger hypoxia response, innate immune responses and metabolic processes. In addition, the transcript levels of the genes involved in the synthetic pathways of antimicrobial metabolites camalexin and coumarin are increased after the E. aerogenes VCs exposure. Moreover, we demonstrate that MKK1 serves as a regulator of camalexin biosynthesis gene expression in response to E. aerogenes VCs, while MKK3 is the regulator of coumarin biosynthesis gene expression. Additionally, MKK1 and MKK3 mediate the E. aerogenes VCs-induced callose deposition. Collectively, these studies provide molecular insights into immune responses by plant-deleterious mVCs.
Keywords	Arabidopsis thaliana ; Enterobacter aerogenes ; auxins ; biosynthesis ; callose ; coumarin ; gene expression ; growth retardation ; hypoxia ; metabolites ; plant growth ; root tips ; transcription (genetics) ; transcriptional activation
Language	English
Dates of publication	2023-01
Size	p. 21-36.
Publishing place	Springer Netherlands
Document type	Article ; Online
ZDB-ID	778032-1
ISSN	1573-5028 ; 0167-4412
ISSN (online)	1573-5028
ISSN	0167-4412
DOI	10.1007/s11103-022-01308-2
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Article ; Online: Antifungal mechanism of volatile compounds emitted by

Nguyen, Tri-Phuong / Meng, De-Rui / Chang, Ching-Han / Su, Pei-Yu / Ou, Chieh-An / Hou, Ping-Fu / Sung, Huang-Mo / Chou, Chang-Hung / Ohme-Takagi, Masaru / Huang, Hao-Jen

mSphere

2023 Volume 8, Issue 5, Page(s) e0032423

Abstract: Increasing evidence suggests that in disease-suppressive soils, microbial volatile compounds (mVCs) released from bacteria may inhibit the growth of plant-pathogenic fungi. However, the antifungal activities and molecular responses of fungi to different ... ...

Abstract	Increasing evidence suggests that in disease-suppressive soils, microbial volatile compounds (mVCs) released from bacteria may inhibit the growth of plant-pathogenic fungi. However, the antifungal activities and molecular responses of fungi to different mVCs remain largely undescribed. In this study, we first evaluated the responses of pathogenic fungi to treatment with mVCs from
MeSH term(s)	Saccharomyces cerevisiae/genetics ; Antifungal Agents/pharmacology ; Soil ; Fungi ; Anti-Infective Agents/pharmacology ; Iron
Chemical Substances	Antifungal Agents ; Soil ; Anti-Infective Agents ; Iron (E1UOL152H7)
Language	English
Publishing date	2023-09-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/msphere.00324-23
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Article ; Online: Volatile Compounds Emitted by Plant Growth-Promoting Fungus Tolypocladium inflatum GT22 Alleviate Copper and Pathogen Stress.

Chiang, Chih-Yun / Chang, Ching-Han / Tseng, Tzu-Yun / Nguyen, Van-Anh Thi / Su, Pei-Yu / Truong, Tu-Trinh Thi / Chen, Jing-Yu / Huang, Chung-Chih / Huang, Hao-Jen

Plant & cell physiology

2023 Volume 65, Issue 2, Page(s) 199–215

Abstract: Previous studies on the intricate interactions between plants and microorganisms have revealed that fungal volatile compounds (VCs) can affect plant growth and development. However, the precise mechanisms underlying these actions remain to be delineated. ...

Abstract	Previous studies on the intricate interactions between plants and microorganisms have revealed that fungal volatile compounds (VCs) can affect plant growth and development. However, the precise mechanisms underlying these actions remain to be delineated. In this study, we discovered that VCs from the soilborne fungus Tolypocladium inflatum GT22 enhance the growth of Arabidopsis. Remarkably, priming Arabidopsis with GT22 VCs caused the plant to display an enhanced immune response and mitigated the detrimental effects of both pathogenic infections and copper stress. Transcriptomic analyses of Arabidopsis seedlings treated with GT22 VCs for 3, 24 and 48 h revealed that 90, 83 and 137 genes were differentially expressed, respectively. The responsive genes are known to be involved in growth, hormone regulation, defense mechanisms and signaling pathways. Furthermore, we observed the induction of genes related to innate immunity, hypoxia, salicylic acid biosynthesis and camalexin biosynthesis by GT22 VCs. Among the VCs emitted by GT22, exposure of Arabidopsis seedlings to limonene promoted plant growth and attenuated copper stress. Thus, limonene appears to be a key mediator of the interaction between GT22 and plants. Overall, our findings provide evidence that fungal VCs can promote plant growth and enhance both biotic and abiotic tolerance. As such, our study suggests that exposure of seedlings to T. inflatum GT22 VCs may be a means of improving crop productivity. This study describes a beneficial interaction between T. inflatun GT22 and Arabidopsis. Our investigation of microorganism function in terms of VC activities allowed us to overcome the limitations of traditional microbial application methods. The importance of this study lies in the discovery of T. inflatun GT22 as a beneficial microorganism. This soilborne fungus emits VCs with plant growth-promoting effects and the ability to alleviate both copper and pathogenic stress. Furthermore, our study offers a valuable approach to tracking the activities of fungal VC components via transcriptomic analysis and sheds light on the mechanisms through which VCs promote plant growth and induce resistance. This research significantly advances our knowledge of VC applications and provides an example for further investigations within this field.
MeSH term(s)	Arabidopsis/genetics ; Copper/pharmacology ; Copper/metabolism ; Limonene/metabolism ; Limonene/pharmacology ; Hypocreales/metabolism ; Plants/metabolism ; Seedlings/metabolism ; Gene Expression Regulation, Plant
Chemical Substances	Copper (789U1901C5) ; Limonene (9MC3I34447)
Language	English
Publishing date	2023-11-09
Publishing country	Japan
Document type	Journal Article
ZDB-ID	208907-5
ISSN	1471-9053 ; 0032-0781
ISSN (online)	1471-9053
ISSN	0032-0781
DOI	10.1093/pcp/pcad120
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Article ; Online: Uncovering molecular mechanisms involved in microbial volatile compounds-induced stomatal closure in Arabidopsis thaliana.

Truong, Tu-Trinh Thi / Chiu, Chi-Chou / Chen, Jing-Yu / Su, Pei-Yu / Nguyen, Tri-Phuong / Trinh, Ngoc-Nam / Mimura, Tetsuro / Lee, Ruey-Hua / Chang, Ching-Han / Huang, Hao-Jen

Plant molecular biology

2023 Volume 113, Issue 4-5, Page(s) 143–155

Abstract: Microbial volatile compounds (mVCs) may cause stomatal closure to limit pathogen invasion as part of plant innate immune response. However, the mechanisms of mVC-induced stomatal closure remain unclear. In this study, we co-cultured Enterobacter ... ...

Abstract	Microbial volatile compounds (mVCs) may cause stomatal closure to limit pathogen invasion as part of plant innate immune response. However, the mechanisms of mVC-induced stomatal closure remain unclear. In this study, we co-cultured Enterobacter aerogenes with Arabidopsis (Arabidopsis thaliana) seedlings without direct contact to initiate stomatal closure. Experiments using the reactive oxygen species (ROS)-sensitive fluorescent dye, H
MeSH term(s)	Arabidopsis/physiology ; Arabidopsis Proteins/genetics ; Abscisic Acid/pharmacology ; Reactive Oxygen Species ; Plant Stomata/physiology
Chemical Substances	Arabidopsis Proteins ; Abscisic Acid (72S9A8J5GW) ; Reactive Oxygen Species
Language	English
Publishing date	2023-11-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	778032-1
ISSN	1573-5028 ; 0167-4412
ISSN (online)	1573-5028
ISSN	0167-4412
DOI	10.1007/s11103-023-01379-9
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Article ; Online: Repairing TALEN-mediated double-strand break by microhomology-mediated recombination in tobacco plastids generates abundant subgenomic DNA.

Huang, Chih-Hao / Liu, Yu-Chang / Shen, Jia-Yi / Lu, Fu-I / Shaw, Shyh-Yu / Huang, Hao-Jen / Chang, Ching-Chun

Plant science : an international journal of experimental plant biology

2021 Volume 313, Page(s) 111028

Abstract: Transcription activator-like effector nuclease (TALEN) technology has been widely used to edit nuclear genomes in plants but rarely for editing organellar genomes. In addition, ciprofloxacin, commonly used to cause the double-strand break of organellar ... ...

Abstract	Transcription activator-like effector nuclease (TALEN) technology has been widely used to edit nuclear genomes in plants but rarely for editing organellar genomes. In addition, ciprofloxacin, commonly used to cause the double-strand break of organellar DNA for studying the repair mechanism in plants, confers no organellar selectivity and site-specificity. To demonstrate the feasibility of TALEN-mediated chloroplast DNA editing and to use it for studying the repair mechanism in plastids, we developed a TALEN-mediated editing technology fused with chloroplast transit peptide (cpTALEN) to site-specifically edit the rpoB gene via Agrobacteria-mediated transformation of tobacco leaf. Transgenic plants showed various degrees of chlorotic phenotype. Repairing damaged plastid DNA resulted in point mutation, large deletion and small inversion surrounding the rpoB gene by homologous recombination and/or microhomology-mediated recombination. In an albino line, microhomology-mediated recombination via a pair of 12-bp direct repeats between rpoC2 and ycf2 genes generated the chimeric ycf2-rpoC2 subgenome, with the level about 3- to 5-fold higher for subgenomic DNA than ycf2. Additionally, the expression of chimeric ycf2-rpoC2 transcripts versus ycf2 mRNA agreed well with the level of corresponding DNA. The ycf2-rpoC2 subgenomic DNA might independently and preferentially replicate in plastids.
MeSH term(s)	DNA Repair ; DNA, Chloroplast ; Gene Editing/methods ; Homologous Recombination ; Phenotype ; Plant Breeding/methods ; Plants, Genetically Modified/genetics ; Nicotiana/genetics ; Transcription Activator-Like Effector Nucleases/genetics
Chemical Substances	DNA, Chloroplast ; Transcription Activator-Like Effector Nucleases (EC 3.1.-)
Language	English
Publishing date	2021-08-24
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	742010-9
ISSN	1873-2259 ; 0168-9452
ISSN (online)	1873-2259
ISSN	0168-9452
DOI	10.1016/j.plantsci.2021.111028
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